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GENTAUR Inc reastain quick-diff kit
Reastain Quick Diff Kit, supplied by GENTAUR Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/diff-quick+kit/reastain+quick+diff+kit/us11993649-303-30-33
Average 90 stars, based on 1 article reviews
reastain quick-diff kit - by Bioz Stars, 2026-09
90/100 stars

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Article Title: Differential roles and regulation of the protein kinases PAK4, PAK5 and PAK6 in melanoma cells
Article Snippet: The chambers were placed at 37°C in 5% CO 2 for 20 h. Non-invading cells in the upper chamber were scraped off and the migrated cells on the bottom of the chamber were fixed and stained using REASTAIN Quick-Diff kit (Gentaur Molecular Products) according to manufacturer's instructions.

Article Title: Inhibition of ErbB kinase signalling promotes resolution of neutrophilic inflammation
Article Snippet: Briefly, for the assessment of apoptosis by light microscopy based on well-characterised morphological changes, neutrophils were cytocentrifuged, fixed with methanol, stained with Reastain Quick-Diff (Gentaur), and then apoptotic and non-apoptotic neutrophils were counted with an inverted, oil immersion microscope (Nikon Eclipse TE300, Japan) at 100X magnification (Savill et al., 1989).

Article Title: Differential roles and regulation of the protein kinases PAK4, PAK5 and PAK6 in melanoma cells
Article Snippet: The chambers were placed at 37°C in 5% CO2 for 20 h. Non-invading cells in the upper chamber were scraped off and the migrated cells on the bottom of the chamber were fixed and stained using REASTAIN Quick-Diff kit (Gentaur Molecular Products) according to manufacturer’s instructions.

Article Title: Neutrophils do not impact viral load or the peak of disease severity during RSV infection
Article Snippet: Slides were H&E stained using Reastain Quick-Diff kit (Gentaur), according to the manufacturer’s instructions.

Article Title: Ageing results in an exacerbated inflammatory response to LPS by resident lung cells.
Article Snippet: For cytospin preparations, BAL cells were centrifuged onto glass slides at 450 rpm for 5 min using a Cytospin 4 cytocentrifuge, then fixed and stained using the Reastain Quick-Diff kit (GENTAUR Ltd), according to manufacturer’s instructions.

Article Title: Neutrophilic inflammation in the respiratory mucosa predisposes to RSV infection.
Article Snippet: INTRODUCTION: Even with intimate exposure to a virus, some people fail to become infected.. Variable transmission partly depends on the dose and duration of exposure but is also governed by the immune status of the host, such as the presence of specific protective antibodies or T cells.. However, for some infections, the reasons for erratic transmission are largely unknown.

Article Title: Antibodies against MFAP4
Article Snippet: After 3.5 hours, the non-migrated cells were removed by swiping a cotton bud gently on the upper surface followed by wash in PBS and then the filter was stained with Reastain Quick-Diff kit (Gentaur Molecular Products).

Staining:

Article Title: Differential roles and regulation of the protein kinases PAK4, PAK5 and PAK6 in melanoma cells
Article Snippet: .. After 20 h, unmigrated cells on top of the chamber were scraped off and the cells at the bottom of the transwell were fixed and stained using diff quick stain kit (Gentaur). ..

Diff-Quik:

Article Title: Differential roles and regulation of the protein kinases PAK4, PAK5 and PAK6 in melanoma cells
Article Snippet: .. After 20 h, unmigrated cells on top of the chamber were scraped off and the cells at the bottom of the transwell were fixed and stained using diff quick stain kit (Gentaur). ..



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RBM23 knockdown inhibits colon cancer cell growth and survival (A) Protein was extracted from the normal colon cell lines CCD-18Co and colon cancer cell lines (HCT116, HT29, LOVO, LS513, DLD-1, COLO205, HCT15) and quantified in equal amounts, and then the protein expression of RBM23 was detected by western blotting. (B) The mRNA expression level of RBM23 was measured by qRT-PCR in the normal colon cell line CCD-18Co and colon cancer cell lines (DLD-1, HCT15, HT29, HCT116). GAPDH was used as an internal control for normalization. (C) Western blot analysis was performed to measure the expression levels of c-Myc in HCT15 cells treated with control siRNA or RBM23 siRNA for 72 h. (D) HCT116 cells were treated with control siRNA or RBM23 siRNA #1, #2, #3 for 72 h. Protein expression levels of various factors were then analyzed by western blotting. (E) The effect of RBM23 deficiency on HCT116, a colon cancer cell line. HCT116 was transfected with 80 nM RBM23 siRNA and control siRNA, and cell viability was examined at 0 and 72 h of treatment. Cell viability was measured by CCK8 assay and optical density was measured by microplate reader at 450 nm. Data were normalized to the control siRNA group at 0 h (set as 100%). (F) The effect of RBM23 knockdown in colon cancer cells, HCT116, on cell proliferation. HCT116 cells were transfected with RBM23 siRNA (100 nM) and incubated for one week, and colonies were stained with Diff Quick solution. The colonies were counted and quantified. (G) Western blotting was performed to detect proteins extracted from HCT116 cells fractionated into nucleus and cytoplasm. Proteins from HCT116 cells transfected with RBM23 siRNA (100 nM) for 72 h were extracted and separated by NE-PER nuclear and cytoplasmic extraction kit into proteins extracted from the nucleus and cytoplasm. The proteins were then detected using western blotting. Student’s t -test: ***P < 0.001

Journal: Cancer Cell International

Article Title: Inhibition of RBM23 induces ferroptosis in colon cancer cells via c-Myc regulation

doi: 10.1186/s12935-026-04262-x

Figure Lengend Snippet: RBM23 knockdown inhibits colon cancer cell growth and survival (A) Protein was extracted from the normal colon cell lines CCD-18Co and colon cancer cell lines (HCT116, HT29, LOVO, LS513, DLD-1, COLO205, HCT15) and quantified in equal amounts, and then the protein expression of RBM23 was detected by western blotting. (B) The mRNA expression level of RBM23 was measured by qRT-PCR in the normal colon cell line CCD-18Co and colon cancer cell lines (DLD-1, HCT15, HT29, HCT116). GAPDH was used as an internal control for normalization. (C) Western blot analysis was performed to measure the expression levels of c-Myc in HCT15 cells treated with control siRNA or RBM23 siRNA for 72 h. (D) HCT116 cells were treated with control siRNA or RBM23 siRNA #1, #2, #3 for 72 h. Protein expression levels of various factors were then analyzed by western blotting. (E) The effect of RBM23 deficiency on HCT116, a colon cancer cell line. HCT116 was transfected with 80 nM RBM23 siRNA and control siRNA, and cell viability was examined at 0 and 72 h of treatment. Cell viability was measured by CCK8 assay and optical density was measured by microplate reader at 450 nm. Data were normalized to the control siRNA group at 0 h (set as 100%). (F) The effect of RBM23 knockdown in colon cancer cells, HCT116, on cell proliferation. HCT116 cells were transfected with RBM23 siRNA (100 nM) and incubated for one week, and colonies were stained with Diff Quick solution. The colonies were counted and quantified. (G) Western blotting was performed to detect proteins extracted from HCT116 cells fractionated into nucleus and cytoplasm. Proteins from HCT116 cells transfected with RBM23 siRNA (100 nM) for 72 h were extracted and separated by NE-PER nuclear and cytoplasmic extraction kit into proteins extracted from the nucleus and cytoplasm. The proteins were then detected using western blotting. Student’s t -test: ***P < 0.001

Article Snippet: The plates were then incubated at 37 °C, 5% CO 2 for 1 week and the colonies were fixed and stained using Diff-Quick Kit (Sysmex Corporation, Kobe, Hyogo, Japan) and the number of colonies was counted.

Techniques: Knockdown, Expressing, Western Blot, Quantitative RT-PCR, Control, Transfection, CCK-8 Assay, Incubation, Staining, Diff-Quik, Extraction