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reastain quick-diff kit (Reagena Ltd)

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Reagena Ltd reastain quick-diff kit
Reastain Quick Diff Kit, supplied by Reagena Ltd, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/diff-quick+kit/reastain+quick+diff+kit/pmc02822953-27-40-43
Average 90 stars, based on 1 article reviews

reastain quick-diff kit - by Bioz Stars, 2026-09

90/100 stars

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Article Title: Integrative Omic Profiling Reveals Unique Hypoxia Induced Signatures in Gastric Cancer Associated Myofibroblasts
Article Snippet: Cells migrating through the membrane were fixed and detected on the lower surface using Reastain Quick-Diff Kit (Reagena; cat. no. 102164, Toivala, Finland) and then examined under a bright-field microscope.

Article Title: Integrated omics profiling reveals novel patterns of epigenetic programming in cancer-associated myofibroblasts
Article Snippet: Cells migrating through the membrane were fixed and detected on the lower surface using Reastain Quick-Diff Kit (Reagena; cat. no. 102164).

Article Title: Neutrophil GM‐CSF receptor dynamics in acute lung injury
Article Snippet: Cytospins (Shandon Cytospin 3) from BALF were methanol fixed and stained with REASTAIN Quick‐Diff Kit (Reagena, Toivala, Finland).

Article Title: Intratracheal Administration of Nanoplastics With Varying Surface Hydrophobicity Results in Coarsely Vacuolated Alveolar Macrophages, Transient Respiratory Inflammation, and Mild Collagen Deposition
Article Snippet: An aliquot of 100 μLundiluted BAL fluid was used for cytospin and differential cell staining (Reastain Quick-Diff Kit, Reagena Ltd, Finland).

Article Title: MicroRNA Monitoring in Human Alveolar Macrophages from Patients with Smoking-Related Lung Diseases: A Preliminary Study
Article Snippet: Cell viability was determined by Trypan blue and a differential cell count was performed by microscopy on the cytospin slide after staining with QUICK-DIFF KIT (Reastain ® , Reagena Toivala, Finland); at least 100 cells were counted.

Article Title: Induction of cross-reactive cellular response against rhinovirus antigens
Article Snippet: BAL Cell Cytospin Assay BAL cells were spun onto slides using the cytospin 3 system (Shandon, USA) and stained with the Reastain Quick-diff kit (Reagena, Finland).

Article Title: Lentiviral and AAV-mediated expression of palivizumab offer protection against Respiratory Syncytial Virus infection
Article Snippet: The slides were fixed and stained using Reastain Quick-Diff Kit (Reagena, Toivala, Finland) and visualised using a light microscope.

Staining:

Article Title: Major Traumatic Injury and Exposure to Mitochondrial-Derived Damage-Associated Molecular Patterns Promotes Neutrophil Survival Accompanied by Stabilisation of the Anti-Apoptotic Protein Mcl-1.
Article Snippet: Briefly, freshly isolated, CHX, or CHX and mtDAMP treated neutrophils (2 × 105 in CM) were loaded into Cytospin chambers and centrifuged at RT for 5 min at 300 revolutions per minute in a Cytospin 4 bench top centrifuge (ThermoFisher Scientific). .. Post-spin, the samples were air dried for 5 min prior to staining with a Reastain Quick Diff Kit, according to the manufacturer’s guidelines (Reagena, Toivala, Finland). ..

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$RBM23 knockdown inhibits colon cancer cell growth and survival (A) Protein was extracted from the normal colon cell lines CCD-18Co and colon cancer cell lines (HCT116, HT29, LOVO, LS513, DLD-1, COLO205, HCT15) and quantified in equal amounts, and then the protein expression of RBM23 was detected by western blotting. (B) The mRNA expression level of RBM23 was measured by qRT-PCR in the normal colon cell line CCD-18Co and colon cancer cell lines (DLD-1, HCT15, HT29, HCT116). GAPDH was used as an internal control for normalization. (C) Western blot analysis was performed to measure the expression levels of c-Myc in HCT15 cells treated with control siRNA or RBM23 siRNA for 72 h. (D) HCT116 cells were treated with control siRNA or RBM23 siRNA #1, #2, #3 for 72 h. Protein expression levels of various factors were then analyzed by western blotting. (E) The effect of RBM23 deficiency on HCT116, a colon cancer cell line. HCT116 was transfected with 80 nM RBM23 siRNA and control siRNA, and cell viability was examined at 0 and 72 h of treatment. Cell viability was measured by CCK8 assay and optical density was measured by microplate reader at 450 nm. Data were normalized to the control siRNA group at 0 h (set as 100%). (F) The effect of RBM23 knockdown in colon cancer cells, HCT116, on cell proliferation. HCT116 cells were transfected with RBM23 siRNA (100 nM) and incubated for one week, and colonies were stained with Diff Quick solution. The colonies were counted and quantified. (G) Western blotting was performed to detect proteins extracted from HCT116 cells fractionated into nucleus and cytoplasm. Proteins from HCT116 cells transfected with RBM23 siRNA (100 nM) for 72 h were extracted and separated by NE-PER nuclear and cytoplasmic extraction kit into proteins extracted from the nucleus and cytoplasm. The proteins were then detected using western blotting. Student’s t -test: ***P < 0.001$

Journal: Cancer Cell International

Article Title: Inhibition of RBM23 induces ferroptosis in colon cancer cells via c-Myc regulation

doi: 10.1186/s12935-026-04262-x

Figure Lengend Snippet: RBM23 knockdown inhibits colon cancer cell growth and survival (A) Protein was extracted from the normal colon cell lines CCD-18Co and colon cancer cell lines (HCT116, HT29, LOVO, LS513, DLD-1, COLO205, HCT15) and quantified in equal amounts, and then the protein expression of RBM23 was detected by western blotting. (B) The mRNA expression level of RBM23 was measured by qRT-PCR in the normal colon cell line CCD-18Co and colon cancer cell lines (DLD-1, HCT15, HT29, HCT116). GAPDH was used as an internal control for normalization. (C) Western blot analysis was performed to measure the expression levels of c-Myc in HCT15 cells treated with control siRNA or RBM23 siRNA for 72 h. (D) HCT116 cells were treated with control siRNA or RBM23 siRNA #1, #2, #3 for 72 h. Protein expression levels of various factors were then analyzed by western blotting. (E) The effect of RBM23 deficiency on HCT116, a colon cancer cell line. HCT116 was transfected with 80 nM RBM23 siRNA and control siRNA, and cell viability was examined at 0 and 72 h of treatment. Cell viability was measured by CCK8 assay and optical density was measured by microplate reader at 450 nm. Data were normalized to the control siRNA group at 0 h (set as 100%). (F) The effect of RBM23 knockdown in colon cancer cells, HCT116, on cell proliferation. HCT116 cells were transfected with RBM23 siRNA (100 nM) and incubated for one week, and colonies were stained with Diff Quick solution. The colonies were counted and quantified. (G) Western blotting was performed to detect proteins extracted from HCT116 cells fractionated into nucleus and cytoplasm. Proteins from HCT116 cells transfected with RBM23 siRNA (100 nM) for 72 h were extracted and separated by NE-PER nuclear and cytoplasmic extraction kit into proteins extracted from the nucleus and cytoplasm. The proteins were then detected using western blotting. Student’s t -test: ***P < 0.001

Article Snippet: The plates were then incubated at 37 °C, 5% CO 2 for 1 week and the colonies were fixed and stained using Diff-Quick Kit (Sysmex Corporation, Kobe, Hyogo, Japan) and the number of colonies was counted.

Techniques: Knockdown, Expressing, Western Blot, Quantitative RT-PCR, Control, Transfection, CCK-8 Assay, Incubation, Staining, Diff-Quik, Extraction