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diff-quick staining kit (Polysciences inc)

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Polysciences inc diff-quick staining kit
Diff Quick Staining Kit, supplied by Polysciences inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/diff-quick+kit/diff+quick+staining+kit/pmc07602396-290-17-20
Average 90 stars, based on 1 article reviews

diff-quick staining kit - by Bioz Stars, 2026-11

90/100 stars

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Article Title: FGFR4 Inhibitor BLU9931 Attenuates Pancreatic Cancer Cell Proliferation and Invasion While Inducing Senescence: Evidence for Senolytic Therapy Potential in Pancreatic Cancer
Article Snippet: Six hour later, cells that had migrated through the membrane pores were fixed and stained with a Diff-Quick staining kit (Polysciences, Inc., Warrington, PA, USA), and counted under a light microscopy.

Article Title: MiR-7-5p inhibits thyroid cell proliferation by targeting the EGFR/MAPK and IRS2/PI3K signaling pathways
Article Snippet: 22-hours after seeding into the upper chamber, passing cells were fixed, stained by using Diff-Quick stain kit (Polysciences) and quantified by counting and averaging 5 separate spots of 4 mm diameter.

Article Title: Prevention of lung injury by Muc1 mucin in a mouse model of repetitive Pseudomonas aeruginosa infection
Article Snippet: Differential cell counting of BALF BALF was subjected to cytocentrifugation and cells were stained with a modified Giemsa stain protocol using the Diff-Quick staining kit (Polysciences, Inc., Warrington, PA).

Article Title: Prevention of lung injury by Muc1 mucin in a mouse model of repetitive Pseudomonas aeruginosa infection
Article Snippet: BALF was subjected to cytocentrifugation and cells were stained with a modified Giemsa stain protocol using the Diff-Quick staining kit (Polysciences, Inc., Warrington, PA).

Article Title: Serum Amyloid P Contained in Alveolar Fluid From Patients With Acute Respiratory Distress Syndrome Mediates the Inhibition of Monocyte Differentiation into Fibrocyte
Article Snippet: Critical Care Medicine www.ccmjournal.org e563 Objective: Alveolar fibrocytes are monocyte-derived mesenchymal cells associated with poor prognosis in patients with acute respiratory distress syndrome.. Our aims were to determine the following: 1) the ability of monocytes from acute respiratory distress syndrome patients to differentiate into fibrocytes; 2) the influence of the acute respiratory distress syndrome alveolar environment on fibrocyte differentiation; and 3) mediators involved in this modulation, focusing on serum amyloid P. Design: Experimental in vitro investigation.. Setting: Two ICUs of a teaching hospital.

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Article Title: Potential impacts of historical disturbance on green turtle health in the unique & protected marine ecosystem of Palmyra Atoll (Central Pacific).
Article Snippet: Palmyra Atoll, in the Central Pacific, is a unique marine ecosystem because of its remarkably intact food web and limited anthropogenic stressors.. However during World War II the atoll was structurally reconfigured into a military installation and questions remain whether this may have impacted the health of the atoll’s ecosystems and species.. To address the issue we assessed green sea turtle (n = 157) health and exposure to contaminants at this foraging ground from 2008 to 2012.

Article Title: New Host Range for Hematodinium in Southern Australia and Novel Tools for Sensitive Detection of Parasitic Dinoflagellates
Article Snippet: Slides were immediately fixed and stained using a Diff-quick staining kit (Polysciences) according to the manufacturers instructions and mounted under a coverslip for microscopic examination with a 100X oil immersion lens. .. Slides were immediately fixed and stained using a Diff-quick ® staining kit (Polysciences) according to the manufacturers instructions and mounted under a coverslip for microscopic examination with a 100X oil immersion lens. .. 50 μL of haemolymph was combined with 150 μl of DNAzol (Invitrogen) and 150 μl of chloroform, vortexed, incubated for 5 minutes at room temperature and centrifuged for 10 minutes at 12,000 g to remove debris.

Article Title: Partial Complement Factor H Deficiency Associates with C3 Glomerulopathy and Thrombotic Microangiopathy
Article Snippet: Hematocrit was calculated after centrifuging EDTA-treated blood in ammonium-heparin capillary tubes (VWR International Ltd., Lutterworth, UK) at 13,000 rpm for 5 minutes and measuring the packed cell height to total height ratio. .. Peripheral blood smears were prepared on glass slides and stained using the Diff Quick Staining Kit (Polysciences, Inc., Warrington, PA). .. Hematuria was detected using Hema-Combistix Urine Reagent Strips (Siemens, Frimley, UK) using the following scale: negative, trace, 1+, 2+, and 3+.

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$RBM23 knockdown inhibits colon cancer cell growth and survival (A) Protein was extracted from the normal colon cell lines CCD-18Co and colon cancer cell lines (HCT116, HT29, LOVO, LS513, DLD-1, COLO205, HCT15) and quantified in equal amounts, and then the protein expression of RBM23 was detected by western blotting. (B) The mRNA expression level of RBM23 was measured by qRT-PCR in the normal colon cell line CCD-18Co and colon cancer cell lines (DLD-1, HCT15, HT29, HCT116). GAPDH was used as an internal control for normalization. (C) Western blot analysis was performed to measure the expression levels of c-Myc in HCT15 cells treated with control siRNA or RBM23 siRNA for 72 h. (D) HCT116 cells were treated with control siRNA or RBM23 siRNA #1, #2, #3 for 72 h. Protein expression levels of various factors were then analyzed by western blotting. (E) The effect of RBM23 deficiency on HCT116, a colon cancer cell line. HCT116 was transfected with 80 nM RBM23 siRNA and control siRNA, and cell viability was examined at 0 and 72 h of treatment. Cell viability was measured by CCK8 assay and optical density was measured by microplate reader at 450 nm. Data were normalized to the control siRNA group at 0 h (set as 100%). (F) The effect of RBM23 knockdown in colon cancer cells, HCT116, on cell proliferation. HCT116 cells were transfected with RBM23 siRNA (100 nM) and incubated for one week, and colonies were stained with Diff Quick solution. The colonies were counted and quantified. (G) Western blotting was performed to detect proteins extracted from HCT116 cells fractionated into nucleus and cytoplasm. Proteins from HCT116 cells transfected with RBM23 siRNA (100 nM) for 72 h were extracted and separated by NE-PER nuclear and cytoplasmic extraction kit into proteins extracted from the nucleus and cytoplasm. The proteins were then detected using western blotting. Student’s t -test: ***P < 0.001$

Journal: Cancer Cell International

Article Title: Inhibition of RBM23 induces ferroptosis in colon cancer cells via c-Myc regulation

doi: 10.1186/s12935-026-04262-x

Figure Lengend Snippet: RBM23 knockdown inhibits colon cancer cell growth and survival (A) Protein was extracted from the normal colon cell lines CCD-18Co and colon cancer cell lines (HCT116, HT29, LOVO, LS513, DLD-1, COLO205, HCT15) and quantified in equal amounts, and then the protein expression of RBM23 was detected by western blotting. (B) The mRNA expression level of RBM23 was measured by qRT-PCR in the normal colon cell line CCD-18Co and colon cancer cell lines (DLD-1, HCT15, HT29, HCT116). GAPDH was used as an internal control for normalization. (C) Western blot analysis was performed to measure the expression levels of c-Myc in HCT15 cells treated with control siRNA or RBM23 siRNA for 72 h. (D) HCT116 cells were treated with control siRNA or RBM23 siRNA #1, #2, #3 for 72 h. Protein expression levels of various factors were then analyzed by western blotting. (E) The effect of RBM23 deficiency on HCT116, a colon cancer cell line. HCT116 was transfected with 80 nM RBM23 siRNA and control siRNA, and cell viability was examined at 0 and 72 h of treatment. Cell viability was measured by CCK8 assay and optical density was measured by microplate reader at 450 nm. Data were normalized to the control siRNA group at 0 h (set as 100%). (F) The effect of RBM23 knockdown in colon cancer cells, HCT116, on cell proliferation. HCT116 cells were transfected with RBM23 siRNA (100 nM) and incubated for one week, and colonies were stained with Diff Quick solution. The colonies were counted and quantified. (G) Western blotting was performed to detect proteins extracted from HCT116 cells fractionated into nucleus and cytoplasm. Proteins from HCT116 cells transfected with RBM23 siRNA (100 nM) for 72 h were extracted and separated by NE-PER nuclear and cytoplasmic extraction kit into proteins extracted from the nucleus and cytoplasm. The proteins were then detected using western blotting. Student’s t -test: ***P < 0.001

Article Snippet: The plates were then incubated at 37 °C, 5% CO 2 for 1 week and the colonies were fixed and stained using Diff-Quick Kit (Sysmex Corporation, Kobe, Hyogo, Japan) and the number of colonies was counted.

Techniques: Knockdown, Expressing, Western Blot, Quantitative RT-PCR, Control, Transfection, CCK-8 Assay, Incubation, Staining, Diff-Quik, Extraction