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Becton Dickinson diff-quick kit
Diff Quick Kit, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/diff-quick+kit/diff+quick+kit/pmc11338773-88-13-15
Average 90 stars, based on 1 article reviews
diff-quick kit - by Bioz Stars, 2026-09
90/100 stars

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Related Articles

Staining:

Article Title: Glycolysis-derived acidic microenvironment as a driver of endothelial dysfunction in systemic sclerosis.
Article Snippet: Objectives.. SSc is an autoimmune disease characterized by peripheral vasculopathy and skin and internal organ fibrosis.. Accumulating evidence underlines a close association between a metabolic reprogramming of activated fibroblasts and fibrosis.

Article Title: The critical role of glutamine and fatty acids in the metabolic reprogramming of anoikis -resistant melanoma cells
Article Snippet: Cells on the lower side of the membranes were then stained using the Diff-Quick kit (BD Biosciences) and photographs of randomly chosen fields were taken.

Article Title: Anoikis Resistance as a Further Trait of Acidic-Adapted Melanoma Cells
Article Snippet: Cells on the lower side of the membranes were then stained using the Diff-Quick kit (BD Biosciences) and photographs of randomly chosen fields are taken.

Article Title: Nintedanib-αVβ6 Integrin Ligand Conjugates Reduce TGF β -Induced EMT in Human Non-Small Cell Lung Cancer
Article Snippet: After incubation, the non-invading cells on the upper surface of the insert were wiped off mechanically with a cotton swab, while migrated cells on the lower side of the inserts were fixed in ice-cold methanol for 20 min, and then stained using the Diff-Quick kit (BD Biosciences, Eysins Switzerland).

Article Title: Bioactive Ent-Kaurane Diterpenes Oridonin and Irudonin Prevent Cancer Cells Migration by Interacting with the Actin Cytoskeleton Controller Ezrin.
Article Snippet: After 24 h, cells in the upper chamber were removed with the cotton swab, and the cells at the bottom of the filters were fixed and stained with a Diff-Quick kit (cat. n. B4132-1A, Becton-Dickinson).

Article Title: Mesenchymal to amoeboid transition is associated with stem-like features of melanoma cells
Article Snippet: The filters were then stained using the Diff-Quick kit (BD Biosciences) and photographs of randomly chosen fields are taken.

Article Title: (-)-Epigallocatechin-3-Gallate Prevents IL-1β-Induced uPAR Expression and Invasiveness via the Suppression of NF-κB and AP-1 in Human Bladder Cancer Cells.
Article Snippet: Twenty-four hours after incubation, non-invading cells on the upper surface of the membrane were scraped off using a cotton swab, and invading cells on the lower surface of the membrane were stained with a Diff-Quick kit (Becton-Dickinson, Franklin Lakes, NJ, USA).

Diff-Quik:

Article Title: Glycolysis-derived acidic microenvironment as a driver of endothelial dysfunction in systemic sclerosis.
Article Snippet: Objectives.. SSc is an autoimmune disease characterized by peripheral vasculopathy and skin and internal organ fibrosis.. Accumulating evidence underlines a close association between a metabolic reprogramming of activated fibroblasts and fibrosis.

Article Title: The critical role of glutamine and fatty acids in the metabolic reprogramming of anoikis -resistant melanoma cells
Article Snippet: Cells on the lower side of the membranes were then stained using the Diff-Quick kit (BD Biosciences) and photographs of randomly chosen fields were taken.

Article Title: Anoikis Resistance as a Further Trait of Acidic-Adapted Melanoma Cells
Article Snippet: Cells on the lower side of the membranes were then stained using the Diff-Quick kit (BD Biosciences) and photographs of randomly chosen fields are taken.

Article Title: Nintedanib-αVβ6 Integrin Ligand Conjugates Reduce TGF β -Induced EMT in Human Non-Small Cell Lung Cancer
Article Snippet: After incubation, the non-invading cells on the upper surface of the insert were wiped off mechanically with a cotton swab, while migrated cells on the lower side of the inserts were fixed in ice-cold methanol for 20 min, and then stained using the Diff-Quick kit (BD Biosciences, Eysins Switzerland).

Article Title: Bioactive Ent-Kaurane Diterpenes Oridonin and Irudonin Prevent Cancer Cells Migration by Interacting with the Actin Cytoskeleton Controller Ezrin.
Article Snippet: After 24 h, cells in the upper chamber were removed with the cotton swab, and the cells at the bottom of the filters were fixed and stained with a Diff-Quick kit (cat. n. B4132-1A, Becton-Dickinson).

Article Title: Mesenchymal to amoeboid transition is associated with stem-like features of melanoma cells
Article Snippet: The filters were then stained using the Diff-Quick kit (BD Biosciences) and photographs of randomly chosen fields are taken.

Article Title: (-)-Epigallocatechin-3-Gallate Prevents IL-1β-Induced uPAR Expression and Invasiveness via the Suppression of NF-κB and AP-1 in Human Bladder Cancer Cells.
Article Snippet: Twenty-four hours after incubation, non-invading cells on the upper surface of the membrane were scraped off using a cotton swab, and invading cells on the lower surface of the membrane were stained with a Diff-Quick kit (Becton-Dickinson, Franklin Lakes, NJ, USA).

Incubation:

Article Title: Glycolysis-derived acidic microenvironment as a driver of endothelial dysfunction in systemic sclerosis.
Article Snippet: Objectives.. SSc is an autoimmune disease characterized by peripheral vasculopathy and skin and internal organ fibrosis.. Accumulating evidence underlines a close association between a metabolic reprogramming of activated fibroblasts and fibrosis.

Article Title: The critical role of glutamine and fatty acids in the metabolic reprogramming of anoikis -resistant melanoma cells
Article Snippet: Cells on the lower side of the membranes were then stained using the Diff-Quick kit (BD Biosciences) and photographs of randomly chosen fields were taken.

Article Title: Anoikis Resistance as a Further Trait of Acidic-Adapted Melanoma Cells
Article Snippet: Cells on the lower side of the membranes were then stained using the Diff-Quick kit (BD Biosciences) and photographs of randomly chosen fields are taken.

Article Title: Nintedanib-αVβ6 Integrin Ligand Conjugates Reduce TGF β -Induced EMT in Human Non-Small Cell Lung Cancer
Article Snippet: After incubation, the non-invading cells on the upper surface of the insert were wiped off mechanically with a cotton swab, while migrated cells on the lower side of the inserts were fixed in ice-cold methanol for 20 min, and then stained using the Diff-Quick kit (BD Biosciences, Eysins Switzerland).

Article Title: Bioactive Ent-Kaurane Diterpenes Oridonin and Irudonin Prevent Cancer Cells Migration by Interacting with the Actin Cytoskeleton Controller Ezrin.
Article Snippet: After 24 h, cells in the upper chamber were removed with the cotton swab, and the cells at the bottom of the filters were fixed and stained with a Diff-Quick kit (cat. n. B4132-1A, Becton-Dickinson).

Article Title: Mesenchymal to amoeboid transition is associated with stem-like features of melanoma cells
Article Snippet: The filters were then stained using the Diff-Quick kit (BD Biosciences) and photographs of randomly chosen fields are taken.

Article Title: (-)-Epigallocatechin-3-Gallate Prevents IL-1β-Induced uPAR Expression and Invasiveness via the Suppression of NF-κB and AP-1 in Human Bladder Cancer Cells.
Article Snippet: Twenty-four hours after incubation, non-invading cells on the upper surface of the membrane were scraped off using a cotton swab, and invading cells on the lower surface of the membrane were stained with a Diff-Quick kit (Becton-Dickinson, Franklin Lakes, NJ, USA).

Membrane:

Article Title: Glycolysis-derived acidic microenvironment as a driver of endothelial dysfunction in systemic sclerosis.
Article Snippet: Objectives.. SSc is an autoimmune disease characterized by peripheral vasculopathy and skin and internal organ fibrosis.. Accumulating evidence underlines a close association between a metabolic reprogramming of activated fibroblasts and fibrosis.

Article Title: The critical role of glutamine and fatty acids in the metabolic reprogramming of anoikis -resistant melanoma cells
Article Snippet: Cells on the lower side of the membranes were then stained using the Diff-Quick kit (BD Biosciences) and photographs of randomly chosen fields were taken.

Article Title: Anoikis Resistance as a Further Trait of Acidic-Adapted Melanoma Cells
Article Snippet: Cells on the lower side of the membranes were then stained using the Diff-Quick kit (BD Biosciences) and photographs of randomly chosen fields are taken.

Article Title: Nintedanib-αVβ6 Integrin Ligand Conjugates Reduce TGF β -Induced EMT in Human Non-Small Cell Lung Cancer
Article Snippet: After incubation, the non-invading cells on the upper surface of the insert were wiped off mechanically with a cotton swab, while migrated cells on the lower side of the inserts were fixed in ice-cold methanol for 20 min, and then stained using the Diff-Quick kit (BD Biosciences, Eysins Switzerland).

Article Title: Bioactive Ent-Kaurane Diterpenes Oridonin and Irudonin Prevent Cancer Cells Migration by Interacting with the Actin Cytoskeleton Controller Ezrin.
Article Snippet: After 24 h, cells in the upper chamber were removed with the cotton swab, and the cells at the bottom of the filters were fixed and stained with a Diff-Quick kit (cat. n. B4132-1A, Becton-Dickinson).

Article Title: Mesenchymal to amoeboid transition is associated with stem-like features of melanoma cells
Article Snippet: The filters were then stained using the Diff-Quick kit (BD Biosciences) and photographs of randomly chosen fields are taken.

Article Title: (-)-Epigallocatechin-3-Gallate Prevents IL-1β-Induced uPAR Expression and Invasiveness via the Suppression of NF-κB and AP-1 in Human Bladder Cancer Cells.
Article Snippet: Twenty-four hours after incubation, non-invading cells on the upper surface of the membrane were scraped off using a cotton swab, and invading cells on the lower surface of the membrane were stained with a Diff-Quick kit (Becton-Dickinson, Franklin Lakes, NJ, USA).

Microscopy:

Article Title: Glycolysis-derived acidic microenvironment as a driver of endothelial dysfunction in systemic sclerosis.
Article Snippet: Objectives.. SSc is an autoimmune disease characterized by peripheral vasculopathy and skin and internal organ fibrosis.. Accumulating evidence underlines a close association between a metabolic reprogramming of activated fibroblasts and fibrosis.

Article Title: The critical role of glutamine and fatty acids in the metabolic reprogramming of anoikis -resistant melanoma cells
Article Snippet: Cells on the lower side of the membranes were then stained using the Diff-Quick kit (BD Biosciences) and photographs of randomly chosen fields were taken.

Article Title: Anoikis Resistance as a Further Trait of Acidic-Adapted Melanoma Cells
Article Snippet: Cells on the lower side of the membranes were then stained using the Diff-Quick kit (BD Biosciences) and photographs of randomly chosen fields are taken.

Article Title: Nintedanib-αVβ6 Integrin Ligand Conjugates Reduce TGF β -Induced EMT in Human Non-Small Cell Lung Cancer
Article Snippet: After incubation, the non-invading cells on the upper surface of the insert were wiped off mechanically with a cotton swab, while migrated cells on the lower side of the inserts were fixed in ice-cold methanol for 20 min, and then stained using the Diff-Quick kit (BD Biosciences, Eysins Switzerland).

Article Title: Bioactive Ent-Kaurane Diterpenes Oridonin and Irudonin Prevent Cancer Cells Migration by Interacting with the Actin Cytoskeleton Controller Ezrin.
Article Snippet: After 24 h, cells in the upper chamber were removed with the cotton swab, and the cells at the bottom of the filters were fixed and stained with a Diff-Quick kit (cat. n. B4132-1A, Becton-Dickinson).

Article Title: Mesenchymal to amoeboid transition is associated with stem-like features of melanoma cells
Article Snippet: The filters were then stained using the Diff-Quick kit (BD Biosciences) and photographs of randomly chosen fields are taken.

Article Title: (-)-Epigallocatechin-3-Gallate Prevents IL-1β-Induced uPAR Expression and Invasiveness via the Suppression of NF-κB and AP-1 in Human Bladder Cancer Cells.
Article Snippet: Twenty-four hours after incubation, non-invading cells on the upper surface of the membrane were scraped off using a cotton swab, and invading cells on the lower surface of the membrane were stained with a Diff-Quick kit (Becton-Dickinson, Franklin Lakes, NJ, USA).



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RBM23 knockdown inhibits colon cancer cell growth and survival (A) Protein was extracted from the normal colon cell lines CCD-18Co and colon cancer cell lines (HCT116, HT29, LOVO, LS513, DLD-1, COLO205, HCT15) and quantified in equal amounts, and then the protein expression of RBM23 was detected by western blotting. (B) The mRNA expression level of RBM23 was measured by qRT-PCR in the normal colon cell line CCD-18Co and colon cancer cell lines (DLD-1, HCT15, HT29, HCT116). GAPDH was used as an internal control for normalization. (C) Western blot analysis was performed to measure the expression levels of c-Myc in HCT15 cells treated with control siRNA or RBM23 siRNA for 72 h. (D) HCT116 cells were treated with control siRNA or RBM23 siRNA #1, #2, #3 for 72 h. Protein expression levels of various factors were then analyzed by western blotting. (E) The effect of RBM23 deficiency on HCT116, a colon cancer cell line. HCT116 was transfected with 80 nM RBM23 siRNA and control siRNA, and cell viability was examined at 0 and 72 h of treatment. Cell viability was measured by CCK8 assay and optical density was measured by microplate reader at 450 nm. Data were normalized to the control siRNA group at 0 h (set as 100%). (F) The effect of RBM23 knockdown in colon cancer cells, HCT116, on cell proliferation. HCT116 cells were transfected with RBM23 siRNA (100 nM) and incubated for one week, and colonies were stained with <t>Diff</t> <t>Quick</t> solution. The colonies were counted and quantified. (G) Western blotting was performed to detect proteins extracted from HCT116 cells fractionated into nucleus and cytoplasm. Proteins from HCT116 cells transfected with RBM23 siRNA (100 nM) for 72 h were extracted and separated by NE-PER nuclear and cytoplasmic extraction kit into proteins extracted from the nucleus and cytoplasm. The proteins were then detected using western blotting. Student’s t -test: ***P < 0.001
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RBM23 knockdown inhibits colon cancer cell growth and survival (A) Protein was extracted from the normal colon cell lines CCD-18Co and colon cancer cell lines (HCT116, HT29, LOVO, LS513, DLD-1, COLO205, HCT15) and quantified in equal amounts, and then the protein expression of RBM23 was detected by western blotting. (B) The mRNA expression level of RBM23 was measured by qRT-PCR in the normal colon cell line CCD-18Co and colon cancer cell lines (DLD-1, HCT15, HT29, HCT116). GAPDH was used as an internal control for normalization. (C) Western blot analysis was performed to measure the expression levels of c-Myc in HCT15 cells treated with control siRNA or RBM23 siRNA for 72 h. (D) HCT116 cells were treated with control siRNA or RBM23 siRNA #1, #2, #3 for 72 h. Protein expression levels of various factors were then analyzed by western blotting. (E) The effect of RBM23 deficiency on HCT116, a colon cancer cell line. HCT116 was transfected with 80 nM RBM23 siRNA and control siRNA, and cell viability was examined at 0 and 72 h of treatment. Cell viability was measured by CCK8 assay and optical density was measured by microplate reader at 450 nm. Data were normalized to the control siRNA group at 0 h (set as 100%). (F) The effect of RBM23 knockdown in colon cancer cells, HCT116, on cell proliferation. HCT116 cells were transfected with RBM23 siRNA (100 nM) and incubated for one week, and colonies were stained with <t>Diff</t> <t>Quick</t> solution. The colonies were counted and quantified. (G) Western blotting was performed to detect proteins extracted from HCT116 cells fractionated into nucleus and cytoplasm. Proteins from HCT116 cells transfected with RBM23 siRNA (100 nM) for 72 h were extracted and separated by NE-PER nuclear and cytoplasmic extraction kit into proteins extracted from the nucleus and cytoplasm. The proteins were then detected using western blotting. Student’s t -test: ***P < 0.001
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RBM23 knockdown inhibits colon cancer cell growth and survival (A) Protein was extracted from the normal colon cell lines CCD-18Co and colon cancer cell lines (HCT116, HT29, LOVO, LS513, DLD-1, COLO205, HCT15) and quantified in equal amounts, and then the protein expression of RBM23 was detected by western blotting. (B) The mRNA expression level of RBM23 was measured by qRT-PCR in the normal colon cell line CCD-18Co and colon cancer cell lines (DLD-1, HCT15, HT29, HCT116). GAPDH was used as an internal control for normalization. (C) Western blot analysis was performed to measure the expression levels of c-Myc in HCT15 cells treated with control siRNA or RBM23 siRNA for 72 h. (D) HCT116 cells were treated with control siRNA or RBM23 siRNA #1, #2, #3 for 72 h. Protein expression levels of various factors were then analyzed by western blotting. (E) The effect of RBM23 deficiency on HCT116, a colon cancer cell line. HCT116 was transfected with 80 nM RBM23 siRNA and control siRNA, and cell viability was examined at 0 and 72 h of treatment. Cell viability was measured by CCK8 assay and optical density was measured by microplate reader at 450 nm. Data were normalized to the control siRNA group at 0 h (set as 100%). (F) The effect of RBM23 knockdown in colon cancer cells, HCT116, on cell proliferation. HCT116 cells were transfected with RBM23 siRNA (100 nM) and incubated for one week, and colonies were stained with <t>Diff</t> <t>Quick</t> solution. The colonies were counted and quantified. (G) Western blotting was performed to detect proteins extracted from HCT116 cells fractionated into nucleus and cytoplasm. Proteins from HCT116 cells transfected with RBM23 siRNA (100 nM) for 72 h were extracted and separated by NE-PER nuclear and cytoplasmic extraction kit into proteins extracted from the nucleus and cytoplasm. The proteins were then detected using western blotting. Student’s t -test: ***P < 0.001
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Image Search Results


RBM23 knockdown inhibits colon cancer cell growth and survival (A) Protein was extracted from the normal colon cell lines CCD-18Co and colon cancer cell lines (HCT116, HT29, LOVO, LS513, DLD-1, COLO205, HCT15) and quantified in equal amounts, and then the protein expression of RBM23 was detected by western blotting. (B) The mRNA expression level of RBM23 was measured by qRT-PCR in the normal colon cell line CCD-18Co and colon cancer cell lines (DLD-1, HCT15, HT29, HCT116). GAPDH was used as an internal control for normalization. (C) Western blot analysis was performed to measure the expression levels of c-Myc in HCT15 cells treated with control siRNA or RBM23 siRNA for 72 h. (D) HCT116 cells were treated with control siRNA or RBM23 siRNA #1, #2, #3 for 72 h. Protein expression levels of various factors were then analyzed by western blotting. (E) The effect of RBM23 deficiency on HCT116, a colon cancer cell line. HCT116 was transfected with 80 nM RBM23 siRNA and control siRNA, and cell viability was examined at 0 and 72 h of treatment. Cell viability was measured by CCK8 assay and optical density was measured by microplate reader at 450 nm. Data were normalized to the control siRNA group at 0 h (set as 100%). (F) The effect of RBM23 knockdown in colon cancer cells, HCT116, on cell proliferation. HCT116 cells were transfected with RBM23 siRNA (100 nM) and incubated for one week, and colonies were stained with Diff Quick solution. The colonies were counted and quantified. (G) Western blotting was performed to detect proteins extracted from HCT116 cells fractionated into nucleus and cytoplasm. Proteins from HCT116 cells transfected with RBM23 siRNA (100 nM) for 72 h were extracted and separated by NE-PER nuclear and cytoplasmic extraction kit into proteins extracted from the nucleus and cytoplasm. The proteins were then detected using western blotting. Student’s t -test: ***P < 0.001

Journal: Cancer Cell International

Article Title: Inhibition of RBM23 induces ferroptosis in colon cancer cells via c-Myc regulation

doi: 10.1186/s12935-026-04262-x

Figure Lengend Snippet: RBM23 knockdown inhibits colon cancer cell growth and survival (A) Protein was extracted from the normal colon cell lines CCD-18Co and colon cancer cell lines (HCT116, HT29, LOVO, LS513, DLD-1, COLO205, HCT15) and quantified in equal amounts, and then the protein expression of RBM23 was detected by western blotting. (B) The mRNA expression level of RBM23 was measured by qRT-PCR in the normal colon cell line CCD-18Co and colon cancer cell lines (DLD-1, HCT15, HT29, HCT116). GAPDH was used as an internal control for normalization. (C) Western blot analysis was performed to measure the expression levels of c-Myc in HCT15 cells treated with control siRNA or RBM23 siRNA for 72 h. (D) HCT116 cells were treated with control siRNA or RBM23 siRNA #1, #2, #3 for 72 h. Protein expression levels of various factors were then analyzed by western blotting. (E) The effect of RBM23 deficiency on HCT116, a colon cancer cell line. HCT116 was transfected with 80 nM RBM23 siRNA and control siRNA, and cell viability was examined at 0 and 72 h of treatment. Cell viability was measured by CCK8 assay and optical density was measured by microplate reader at 450 nm. Data were normalized to the control siRNA group at 0 h (set as 100%). (F) The effect of RBM23 knockdown in colon cancer cells, HCT116, on cell proliferation. HCT116 cells were transfected with RBM23 siRNA (100 nM) and incubated for one week, and colonies were stained with Diff Quick solution. The colonies were counted and quantified. (G) Western blotting was performed to detect proteins extracted from HCT116 cells fractionated into nucleus and cytoplasm. Proteins from HCT116 cells transfected with RBM23 siRNA (100 nM) for 72 h were extracted and separated by NE-PER nuclear and cytoplasmic extraction kit into proteins extracted from the nucleus and cytoplasm. The proteins were then detected using western blotting. Student’s t -test: ***P < 0.001

Article Snippet: The plates were then incubated at 37 °C, 5% CO 2 for 1 week and the colonies were fixed and stained using Diff-Quick Kit (Sysmex Corporation, Kobe, Hyogo, Japan) and the number of colonies was counted.

Techniques: Knockdown, Expressing, Western Blot, Quantitative RT-PCR, Control, Transfection, CCK-8 Assay, Incubation, Staining, Diff-Quik, Extraction