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diff-quik stain lt-sys (Technik GmbH)

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Technik GmbH diff-quik stain lt-sys
Diff Quik Stain Lt Sys, supplied by Technik GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/diff-quick+kit/diff+quick+kit/pmc08893648-96-23-30
Average 90 stars, based on 1 article reviews

diff-quik stain lt-sys - by Bioz Stars, 2026-09

90/100 stars

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Article Title: Flowcytometric data of intermediate-large cell gastrointestinal lymphoma presenting a gross mass in 32 cats – “let them glow in the flow”
Article Snippet: The total nucleated cell count (TNCC) was determined by an ADVIA 2120TM (Siemens, Austria) hematology analyzer with the veterinary software setting for cats. .. Cytocentrifuge preparations with approximately 5 × 10 5 cells from the single cell suspensions were stained by a Romanowsky dip stain (LT-Sys Haema-SchnellfaerbungTM, Labor+ Technik, Berlin, Germany). ..

Article Title: Composition of lymphocyte subpopulations in normal and mildly reactive peripheral lymph nodes in cats.
Article Snippet: Objectives Flow cytometric (FCM) immunophenotyping of lymphoid tissue aspirates is an available adjunct for feline lymphoma diagnostics.. Reference data have only been established for feline peripheral blood.. Studies investigating the composition of normal and mildly reactive feline lymph nodes (LNs) are lacking.

other:

Article Title: Immunization with an adeno-associated viral vectored allergy vaccine containing Der p1-Der p2 effectively alleviates an asthmatic phenotype in mice
Article Snippet: Differential cell types were analyzed using the Diff-Quick kit (Labor und Technik Eberhardt Lehmann, Berlin, Germany) for staining.

Article Title: Retrobulbar Adenocarcinoma with Subconjunctival Extension in a Common Buzzard ( Buteo buteo ).
Article Snippet: A free ranging, fledged common buzzard (Buteo buteo) was found with severe feather damage and left periorbital swelling.. Clinical examination revealed a 3.032.531.5 cm left medial subconjunctival mass.. The abnormal tissue extended over most of the left cornea, severely impairing the bird’s vision in that eye.

Article Title: Generating human artery and vein cells from pluripotent stem cells highlights the arterial tropism of Nipah and Hendra viruses.
Article Snippet: Article Generating human artery and vein cells from pluripotent stem cells highlights the arterial tropism of Nipah and Hendra viruses

Article Title: Evaluation of Theileria equi vertical transmission rate and routes in a cohort of asymptomatic mares and their foals.
Article Snippet: The smears were Diff-Quick stained (Labor+ Technik, Berlin, Germany) and read a posteriori to control for the presence of viable parasites in the blood of PCR-positive foals.

Article Title: Chronic cholecystitis: Diagnostic and therapeutic insights from formerly bile-farmed Asiatic black bears ( Ursus thibetanus )
Article Snippet: Direct smears and cytocentrifuge preparations (1000g for 20minutes; [ ]) of the bile were prepared within two hours of sampling and stained using Diff-Quik stain (LT-SYS ® , Labor + Technik, Germany).

Article Title: Clinical Chemistry and Haematology Values of a Captive Population of Humboldt Penguins ( Spheniscus humboldti ).
Article Snippet: Haematology was done using blood smears stained using a Diff-Quick® fast stain kit (Labor+Technik Eberhard Lehmann GmbH, 14167 Berlin, Germany) and evaluated microscopically.

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$RBM23 knockdown inhibits colon cancer cell growth and survival (A) Protein was extracted from the normal colon cell lines CCD-18Co and colon cancer cell lines (HCT116, HT29, LOVO, LS513, DLD-1, COLO205, HCT15) and quantified in equal amounts, and then the protein expression of RBM23 was detected by western blotting. (B) The mRNA expression level of RBM23 was measured by qRT-PCR in the normal colon cell line CCD-18Co and colon cancer cell lines (DLD-1, HCT15, HT29, HCT116). GAPDH was used as an internal control for normalization. (C) Western blot analysis was performed to measure the expression levels of c-Myc in HCT15 cells treated with control siRNA or RBM23 siRNA for 72 h. (D) HCT116 cells were treated with control siRNA or RBM23 siRNA #1, #2, #3 for 72 h. Protein expression levels of various factors were then analyzed by western blotting. (E) The effect of RBM23 deficiency on HCT116, a colon cancer cell line. HCT116 was transfected with 80 nM RBM23 siRNA and control siRNA, and cell viability was examined at 0 and 72 h of treatment. Cell viability was measured by CCK8 assay and optical density was measured by microplate reader at 450 nm. Data were normalized to the control siRNA group at 0 h (set as 100%). (F) The effect of RBM23 knockdown in colon cancer cells, HCT116, on cell proliferation. HCT116 cells were transfected with RBM23 siRNA (100 nM) and incubated for one week, and colonies were stained with Diff Quick solution. The colonies were counted and quantified. (G) Western blotting was performed to detect proteins extracted from HCT116 cells fractionated into nucleus and cytoplasm. Proteins from HCT116 cells transfected with RBM23 siRNA (100 nM) for 72 h were extracted and separated by NE-PER nuclear and cytoplasmic extraction kit into proteins extracted from the nucleus and cytoplasm. The proteins were then detected using western blotting. Student’s t -test: ***P < 0.001$

Journal: Cancer Cell International

Article Title: Inhibition of RBM23 induces ferroptosis in colon cancer cells via c-Myc regulation

doi: 10.1186/s12935-026-04262-x

Figure Lengend Snippet: RBM23 knockdown inhibits colon cancer cell growth and survival (A) Protein was extracted from the normal colon cell lines CCD-18Co and colon cancer cell lines (HCT116, HT29, LOVO, LS513, DLD-1, COLO205, HCT15) and quantified in equal amounts, and then the protein expression of RBM23 was detected by western blotting. (B) The mRNA expression level of RBM23 was measured by qRT-PCR in the normal colon cell line CCD-18Co and colon cancer cell lines (DLD-1, HCT15, HT29, HCT116). GAPDH was used as an internal control for normalization. (C) Western blot analysis was performed to measure the expression levels of c-Myc in HCT15 cells treated with control siRNA or RBM23 siRNA for 72 h. (D) HCT116 cells were treated with control siRNA or RBM23 siRNA #1, #2, #3 for 72 h. Protein expression levels of various factors were then analyzed by western blotting. (E) The effect of RBM23 deficiency on HCT116, a colon cancer cell line. HCT116 was transfected with 80 nM RBM23 siRNA and control siRNA, and cell viability was examined at 0 and 72 h of treatment. Cell viability was measured by CCK8 assay and optical density was measured by microplate reader at 450 nm. Data were normalized to the control siRNA group at 0 h (set as 100%). (F) The effect of RBM23 knockdown in colon cancer cells, HCT116, on cell proliferation. HCT116 cells were transfected with RBM23 siRNA (100 nM) and incubated for one week, and colonies were stained with Diff Quick solution. The colonies were counted and quantified. (G) Western blotting was performed to detect proteins extracted from HCT116 cells fractionated into nucleus and cytoplasm. Proteins from HCT116 cells transfected with RBM23 siRNA (100 nM) for 72 h were extracted and separated by NE-PER nuclear and cytoplasmic extraction kit into proteins extracted from the nucleus and cytoplasm. The proteins were then detected using western blotting. Student’s t -test: ***P < 0.001

Article Snippet: The plates were then incubated at 37 °C, 5% CO 2 for 1 week and the colonies were fixed and stained using Diff-Quick Kit (Sysmex Corporation, Kobe, Hyogo, Japan) and the number of colonies was counted.

Techniques: Knockdown, Expressing, Western Blot, Quantitative RT-PCR, Control, Transfection, CCK-8 Assay, Incubation, Staining, Diff-Quik, Extraction