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Gamidor Technical Services Ltd may-grunwald giemsa stain diff-quick
May Grunwald Giemsa Stain Diff Quick, supplied by Gamidor Technical Services Ltd, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/diff+quick/may+grunwald+giemsa+stain+diff+quick/pm12847277-60-8-12
Average 90 stars, based on 1 article reviews
may-grunwald giemsa stain diff-quick - by Bioz Stars, 2026-09
90/100 stars

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Article Title: Reactive oxygen species limit neutrophil life span by activating death receptor signaling.
Article Snippet: Neutrophils are abundant, short-lived leukocytes, and their death by apoptosis is central to hemostasis and the resolution of inflammation, yet the trigger for their entry into apoptosis is unknown.. We show here that death receptor signaling, including CD95 death-inducing signaling complex (DISC) formation and caspase 8 activation, occurred early in neutrophil apoptosis.. However, death receptor ligation was not required for apoptosis, suggesting a novel mechanism for caspase 8 activation.

Article Title: Interaction between integrin ? 9 ? 1 and vascular cell adhesion molecule-1 (VCAM-1) inhibits neutrophil apoptosis
Article Snippet: Cytospins were then stained using a commercial May-Grunwald Giemsa stain (Diff-Quick; Gamidor, Abingdon, United Kingdom) and assessed for apoptotic morphology using a Zeiss Axiostar Plus microscope equipped with a 100×/1.25 objective lens (Carl Zeiss, Welwyn Garden City, United Kingdom).

Article Title: Characterization of canine monocyte-derived dendritic cells with phenotypic and functional differentiation
Article Snippet: For cytologic study, iDCs were smeared on a glass slide, allowed to air dry, and stained with May-Grünwald Giemsa stain (Diff-Quick; Gamidor, Abingdon, England).

Article Title: Release of Active Peptidyl Arginine Deiminases by Neutrophils Can Explain Production of Extracellular Citrullinated Autoantigens in Rheumatoid Arthritis Synovial Fluid
Article Snippet: SF samples were stained with Giemsa (Diff‐Quick; Gamidor Technical Services).

Article Title: CD5-low expression lymphocytes in canine peripheral blood show characteristics of natural killer cells.
Article Snippet: For cytologic studies, cells from each population were smeared on a glass slide, allowed to air dry, and then stained with May-Grunwald Giemsa stain (Diff-Quick, Gamidor, Abingdon, Oxfordshire, UK).

Article Title: PEP005, a selective small-molecule activator of protein kinase C, has potent antileukemic activity mediated via the delta isoform of PKC.
Article Snippet: Ingenol 3-angelate (PEP005) is a selective small molecule activator of protein kinase C (PKC) extracted from the plant Euphorbia peplus, whose sap has been used as a traditional medicine for the treatment of skin conditions including warts and cancer.. We report here that PEP005 also has potent antileukemic effects, inducing apoptosis in myeloid leukemia cell lines and primary acute myeloid leukemia (AML) cells at nanomolar concentrations.. Of importance, PEP005 did not induce apoptosis in normal CD34 cord blood myeloblasts at up to 2-log concentrations higher than those required to induce cell death in primary AML cells.

Isolation:

Article Title: Lactoferrin is a survival factor for neutrophils in rheumatoid synovial fluid
Article Snippet: After hypotonic lysis to eliminate any remaining erythrocytes, neutrophils were resuspended in a round-bottom polypropylene tube in RPMI 1640 medium (Sigma-Aldrich) supplemented with 10% heat-inactivated fetal bovine serum (Sera Laboratories International, Bolney, UK) and containing 2 mM glutamine, 100 U/ml penicillin and 100 ug/ml streptomycin (Sigma-Aldrich). .. The purity of isolated neutrophils was determined by Giemsa staining (Diff-Quick, Gamidor Technical Services, Didcot, UK), and light microscopy and was routinely >97%, with eosinophils as the main contaminating cell. ..

Staining:

Article Title: Lactoferrin is a survival factor for neutrophils in rheumatoid synovial fluid
Article Snippet: After hypotonic lysis to eliminate any remaining erythrocytes, neutrophils were resuspended in a round-bottom polypropylene tube in RPMI 1640 medium (Sigma-Aldrich) supplemented with 10% heat-inactivated fetal bovine serum (Sera Laboratories International, Bolney, UK) and containing 2 mM glutamine, 100 U/ml penicillin and 100 ug/ml streptomycin (Sigma-Aldrich). .. The purity of isolated neutrophils was determined by Giemsa staining (Diff-Quick, Gamidor Technical Services, Didcot, UK), and light microscopy and was routinely >97%, with eosinophils as the main contaminating cell. ..

Article Title: Platelet-specific P2Y 1 receptor deficient mice have suppressed leukocyte recruitment in response to lipopolysaccharide
Article Snippet: .. Following incubation for 60 min at 37°C, the number of neutrophils that migrated into the bottom chamber was determined by a total cell count combined with a differential cell stain (Diff Quick, Gamidor) to identify neutrophils. ..

Diff-Quik:

Article Title: Lactoferrin is a survival factor for neutrophils in rheumatoid synovial fluid
Article Snippet: After hypotonic lysis to eliminate any remaining erythrocytes, neutrophils were resuspended in a round-bottom polypropylene tube in RPMI 1640 medium (Sigma-Aldrich) supplemented with 10% heat-inactivated fetal bovine serum (Sera Laboratories International, Bolney, UK) and containing 2 mM glutamine, 100 U/ml penicillin and 100 ug/ml streptomycin (Sigma-Aldrich). .. The purity of isolated neutrophils was determined by Giemsa staining (Diff-Quick, Gamidor Technical Services, Didcot, UK), and light microscopy and was routinely >97%, with eosinophils as the main contaminating cell. ..

Article Title: Platelet-specific P2Y 1 receptor deficient mice have suppressed leukocyte recruitment in response to lipopolysaccharide
Article Snippet: .. Following incubation for 60 min at 37°C, the number of neutrophils that migrated into the bottom chamber was determined by a total cell count combined with a differential cell stain (Diff Quick, Gamidor) to identify neutrophils. ..

Light Microscopy:

Article Title: Lactoferrin is a survival factor for neutrophils in rheumatoid synovial fluid
Article Snippet: After hypotonic lysis to eliminate any remaining erythrocytes, neutrophils were resuspended in a round-bottom polypropylene tube in RPMI 1640 medium (Sigma-Aldrich) supplemented with 10% heat-inactivated fetal bovine serum (Sera Laboratories International, Bolney, UK) and containing 2 mM glutamine, 100 U/ml penicillin and 100 ug/ml streptomycin (Sigma-Aldrich). .. The purity of isolated neutrophils was determined by Giemsa staining (Diff-Quick, Gamidor Technical Services, Didcot, UK), and light microscopy and was routinely >97%, with eosinophils as the main contaminating cell. ..

Incubation:

Article Title: Platelet-specific P2Y 1 receptor deficient mice have suppressed leukocyte recruitment in response to lipopolysaccharide
Article Snippet: .. Following incubation for 60 min at 37°C, the number of neutrophils that migrated into the bottom chamber was determined by a total cell count combined with a differential cell stain (Diff Quick, Gamidor) to identify neutrophils. ..

Cell Characterization:

Article Title: Platelet-specific P2Y 1 receptor deficient mice have suppressed leukocyte recruitment in response to lipopolysaccharide
Article Snippet: .. Following incubation for 60 min at 37°C, the number of neutrophils that migrated into the bottom chamber was determined by a total cell count combined with a differential cell stain (Diff Quick, Gamidor) to identify neutrophils. ..



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RBM23 knockdown inhibits colon cancer cell growth and survival (A) Protein was extracted from the normal colon cell lines CCD-18Co and colon cancer cell lines (HCT116, HT29, LOVO, LS513, DLD-1, COLO205, HCT15) and quantified in equal amounts, and then the protein expression of RBM23 was detected by western blotting. (B) The mRNA expression level of RBM23 was measured by qRT-PCR in the normal colon cell line CCD-18Co and colon cancer cell lines (DLD-1, HCT15, HT29, HCT116). GAPDH was used as an internal control for normalization. (C) Western blot analysis was performed to measure the expression levels of c-Myc in HCT15 cells treated with control siRNA or RBM23 siRNA for 72 h. (D) HCT116 cells were treated with control siRNA or RBM23 siRNA #1, #2, #3 for 72 h. Protein expression levels of various factors were then analyzed by western blotting. (E) The effect of RBM23 deficiency on HCT116, a colon cancer cell line. HCT116 was transfected with 80 nM RBM23 siRNA and control siRNA, and cell viability was examined at 0 and 72 h of treatment. Cell viability was measured by CCK8 assay and optical density was measured by microplate reader at 450 nm. Data were normalized to the control siRNA group at 0 h (set as 100%). (F) The effect of RBM23 knockdown in colon cancer cells, HCT116, on cell proliferation. HCT116 cells were transfected with RBM23 siRNA (100 nM) and incubated for one week, and colonies were stained with <t>Diff</t> <t>Quick</t> solution. The colonies were counted and quantified. (G) Western blotting was performed to detect proteins extracted from HCT116 cells fractionated into nucleus and cytoplasm. Proteins from HCT116 cells transfected with RBM23 siRNA (100 nM) for 72 h were extracted and separated by NE-PER nuclear and cytoplasmic extraction kit into proteins extracted from the nucleus and cytoplasm. The proteins were then detected using western blotting. Student’s t -test: ***P < 0.001
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RBM23 knockdown inhibits colon cancer cell growth and survival (A) Protein was extracted from the normal colon cell lines CCD-18Co and colon cancer cell lines (HCT116, HT29, LOVO, LS513, DLD-1, COLO205, HCT15) and quantified in equal amounts, and then the protein expression of RBM23 was detected by western blotting. (B) The mRNA expression level of RBM23 was measured by qRT-PCR in the normal colon cell line CCD-18Co and colon cancer cell lines (DLD-1, HCT15, HT29, HCT116). GAPDH was used as an internal control for normalization. (C) Western blot analysis was performed to measure the expression levels of c-Myc in HCT15 cells treated with control siRNA or RBM23 siRNA for 72 h. (D) HCT116 cells were treated with control siRNA or RBM23 siRNA #1, #2, #3 for 72 h. Protein expression levels of various factors were then analyzed by western blotting. (E) The effect of RBM23 deficiency on HCT116, a colon cancer cell line. HCT116 was transfected with 80 nM RBM23 siRNA and control siRNA, and cell viability was examined at 0 and 72 h of treatment. Cell viability was measured by CCK8 assay and optical density was measured by microplate reader at 450 nm. Data were normalized to the control siRNA group at 0 h (set as 100%). (F) The effect of RBM23 knockdown in colon cancer cells, HCT116, on cell proliferation. HCT116 cells were transfected with RBM23 siRNA (100 nM) and incubated for one week, and colonies were stained with Diff Quick solution. The colonies were counted and quantified. (G) Western blotting was performed to detect proteins extracted from HCT116 cells fractionated into nucleus and cytoplasm. Proteins from HCT116 cells transfected with RBM23 siRNA (100 nM) for 72 h were extracted and separated by NE-PER nuclear and cytoplasmic extraction kit into proteins extracted from the nucleus and cytoplasm. The proteins were then detected using western blotting. Student’s t -test: ***P < 0.001

Journal: Cancer Cell International

Article Title: Inhibition of RBM23 induces ferroptosis in colon cancer cells via c-Myc regulation

doi: 10.1186/s12935-026-04262-x

Figure Lengend Snippet: RBM23 knockdown inhibits colon cancer cell growth and survival (A) Protein was extracted from the normal colon cell lines CCD-18Co and colon cancer cell lines (HCT116, HT29, LOVO, LS513, DLD-1, COLO205, HCT15) and quantified in equal amounts, and then the protein expression of RBM23 was detected by western blotting. (B) The mRNA expression level of RBM23 was measured by qRT-PCR in the normal colon cell line CCD-18Co and colon cancer cell lines (DLD-1, HCT15, HT29, HCT116). GAPDH was used as an internal control for normalization. (C) Western blot analysis was performed to measure the expression levels of c-Myc in HCT15 cells treated with control siRNA or RBM23 siRNA for 72 h. (D) HCT116 cells were treated with control siRNA or RBM23 siRNA #1, #2, #3 for 72 h. Protein expression levels of various factors were then analyzed by western blotting. (E) The effect of RBM23 deficiency on HCT116, a colon cancer cell line. HCT116 was transfected with 80 nM RBM23 siRNA and control siRNA, and cell viability was examined at 0 and 72 h of treatment. Cell viability was measured by CCK8 assay and optical density was measured by microplate reader at 450 nm. Data were normalized to the control siRNA group at 0 h (set as 100%). (F) The effect of RBM23 knockdown in colon cancer cells, HCT116, on cell proliferation. HCT116 cells were transfected with RBM23 siRNA (100 nM) and incubated for one week, and colonies were stained with Diff Quick solution. The colonies were counted and quantified. (G) Western blotting was performed to detect proteins extracted from HCT116 cells fractionated into nucleus and cytoplasm. Proteins from HCT116 cells transfected with RBM23 siRNA (100 nM) for 72 h were extracted and separated by NE-PER nuclear and cytoplasmic extraction kit into proteins extracted from the nucleus and cytoplasm. The proteins were then detected using western blotting. Student’s t -test: ***P < 0.001

Article Snippet: The plates were then incubated at 37 °C, 5% CO 2 for 1 week and the colonies were fixed and stained using Diff-Quick Kit (Sysmex Corporation, Kobe, Hyogo, Japan) and the number of colonies was counted.

Techniques: Knockdown, Expressing, Western Blot, Quantitative RT-PCR, Control, Transfection, CCK-8 Assay, Incubation, Staining, Diff-Quik, Extraction