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diff-quick stain kit  (Imeb Inc)

 
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    Imeb Inc diff-quick stain kit
    Diff Quick Stain Kit, supplied by Imeb Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/diff+quick+kit/diff+quick+staining+kit/pm15257105-37-1-6
    Average 90 stars, based on 1 article reviews
    diff-quick stain kit - by Bioz Stars, 2026-11
    90/100 stars

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    Related Articles

    Staining:

    Article Title: Dynamic WNT signaling controls differentiation of hematopoietic progenitor cells from human pluripotent stem cells.
    Article Snippet: 1Bioscience Program, Biological and Environmental Science and Engineering Division, King Abdullah University of Science and Technology (KAUST), Thuwal 23955-6900, Kingdom of Saudi Arabia 2KAUST Center of Excellence for Smart Health (KCSH), Thuwal 23955-6900, Kingdom of Saudi Arabia 3Gene Expression Laboratory, Salk Institute for Biological Studies, La Jolla 92037, USA 4Institute for Advanced Co-Creation Studies, Osaka University, Osaka 560-8531, Japan 5Integrative Genomics and Bioinformatics Core, Salk Institute for Biological Studies, La Jolla 92037, USA 6Next Generation Sequencing Core, Salk Institute for Biological Studies, La Jolla 92037, USA 7UPMC Univ Paris 06, UMR_S938 CDR Saint-Antoine, Prolifération et Différentiation des Cellules Souches, Paris F-75012, France 8INSERM, UMR_S938, Prolifération et Différentiation des Cellules Souches, Paris F-75012, France 9Center for Regenerative Medicine in Barcelona, Barcelona 08003, Spain 10Key Laboratory of Organ Regeneration and Reconstruction, State Key Laboratory of Membrane Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, China 11Institute for Stem Cell and Regeneration, Chinese Academy of Sciences, Beijing 100101, China 12EFS Ile de France, Unité d’Ingénierie et de Thérapie Cellulaire, Créteil F-94017, France 13AP-HP Hôpital Saint Antoine/Armand Trousseau, Service d’Hématologie et immunologie biologique, Paris F-75012, France 14Altos Labs, San Diego 92122, USA

    Article Title: Protein kinase D1 in myeloid lineage cells contributes to the accumulation of CXCR3 + CCR6 + nonconventional Th1 cells in the lungs and potentiates hypersensitivity pneumonitis caused by S. rectivirgula
    Article Snippet: .. Purity of isolated neutrophils were analyzed by Diff-Quick staining (Diff Quick Stain kit, IMEB Inc., San Marcos, CA) according to the manufacturer’s instructions and approximately 85% to 90% of the isolated cells were morphologically neutrophils/granulocytes. .. RPMI 1640 was purchased from Corning, Pen Strep was purchased from Gibco, and FBS was purchased from R&D Systems (Flowery Branch, GA).

    Article Title: KDM6A Regulates Cell Plasticity and Pancreatic Cancer Progression by Noncanonical Activin Pathway.
    Article Snippet: .. After incubation for 24–48 hours at 37 C, the cells that invaded or migrated through the membrane were fixed and stained with the DIFF staining kit (IMEB Inc., San Marcos, CA) and counted in more than 5 random fields. .. Cells were trypsinized and cultured in a 6-well UltraLow Attachment Plate (ThermoFisher, Waltham, MA) overnight to form spheroids.

    Article Title: Prostate cancer cell-platelet bidirectional signaling promotes calcium mobilization, invasion and apoptotic resistance via distinct receptor-ligand pairs.
    Article Snippet: .. The PCa cell-platelet coculture was incubated for a total of 48 h. PCa cells invading through Matrigel and residing on the bottom side of the PET membrane were fixed, stained (nuclear and cytoplasm) with Diff Stain kit (IMEB Inc., San Marcos, CA), and counted under light microscope. .. Apoptotic activity was measured using the Caspase-Glo 3/7 Assay (Promega) according to manufacturer’s protocol.

    Article Title: Transcriptomic and functional characterization of megakaryocytic-derived platelet-like particles: impaired aggregation and prominent anti-tumor effects.
    Article Snippet: .. Invading PCa cells on the bottom side of the PET membrane were fixed, stained with Diff Stain kit (IMEB Inc.), and counted. .. Apoptotic activity was measured using the Caspase-Glo 3/7 Assay (Promega) according to the manufacturer’s protocol.9 For dose-response studies 20 000 PCa cells were seeded alone or with PLPs at cell ratios of 1:10, 1:100, 1:500, or 1:1000 into 96 well plates for 24 hours.

    Article Title: The Role of Secreted Frizzled-related Protein-1 in Allergic Asthma
    Article Snippet: .. Cytospin preparations were stained with Diff Stain kit (IMEB Inc), and differential cell counts were performed using morphological criteria. ..

    Isolation:

    Article Title: Protein kinase D1 in myeloid lineage cells contributes to the accumulation of CXCR3 + CCR6 + nonconventional Th1 cells in the lungs and potentiates hypersensitivity pneumonitis caused by S. rectivirgula
    Article Snippet: .. Purity of isolated neutrophils were analyzed by Diff-Quick staining (Diff Quick Stain kit, IMEB Inc., San Marcos, CA) according to the manufacturer’s instructions and approximately 85% to 90% of the isolated cells were morphologically neutrophils/granulocytes. .. RPMI 1640 was purchased from Corning, Pen Strep was purchased from Gibco, and FBS was purchased from R&D Systems (Flowery Branch, GA).

    Diff-Quik:

    Article Title: Protein kinase D1 in myeloid lineage cells contributes to the accumulation of CXCR3 + CCR6 + nonconventional Th1 cells in the lungs and potentiates hypersensitivity pneumonitis caused by S. rectivirgula
    Article Snippet: .. Purity of isolated neutrophils were analyzed by Diff-Quick staining (Diff Quick Stain kit, IMEB Inc., San Marcos, CA) according to the manufacturer’s instructions and approximately 85% to 90% of the isolated cells were morphologically neutrophils/granulocytes. .. RPMI 1640 was purchased from Corning, Pen Strep was purchased from Gibco, and FBS was purchased from R&D Systems (Flowery Branch, GA).

    Incubation:

    Article Title: KDM6A Regulates Cell Plasticity and Pancreatic Cancer Progression by Noncanonical Activin Pathway.
    Article Snippet: .. After incubation for 24–48 hours at 37 C, the cells that invaded or migrated through the membrane were fixed and stained with the DIFF staining kit (IMEB Inc., San Marcos, CA) and counted in more than 5 random fields. .. Cells were trypsinized and cultured in a 6-well UltraLow Attachment Plate (ThermoFisher, Waltham, MA) overnight to form spheroids.

    Article Title: Prostate cancer cell-platelet bidirectional signaling promotes calcium mobilization, invasion and apoptotic resistance via distinct receptor-ligand pairs.
    Article Snippet: .. The PCa cell-platelet coculture was incubated for a total of 48 h. PCa cells invading through Matrigel and residing on the bottom side of the PET membrane were fixed, stained (nuclear and cytoplasm) with Diff Stain kit (IMEB Inc., San Marcos, CA), and counted under light microscope. .. Apoptotic activity was measured using the Caspase-Glo 3/7 Assay (Promega) according to manufacturer’s protocol.

    Membrane:

    Article Title: KDM6A Regulates Cell Plasticity and Pancreatic Cancer Progression by Noncanonical Activin Pathway.
    Article Snippet: .. After incubation for 24–48 hours at 37 C, the cells that invaded or migrated through the membrane were fixed and stained with the DIFF staining kit (IMEB Inc., San Marcos, CA) and counted in more than 5 random fields. .. Cells were trypsinized and cultured in a 6-well UltraLow Attachment Plate (ThermoFisher, Waltham, MA) overnight to form spheroids.

    Article Title: Prostate cancer cell-platelet bidirectional signaling promotes calcium mobilization, invasion and apoptotic resistance via distinct receptor-ligand pairs.
    Article Snippet: .. The PCa cell-platelet coculture was incubated for a total of 48 h. PCa cells invading through Matrigel and residing on the bottom side of the PET membrane were fixed, stained (nuclear and cytoplasm) with Diff Stain kit (IMEB Inc., San Marcos, CA), and counted under light microscope. .. Apoptotic activity was measured using the Caspase-Glo 3/7 Assay (Promega) according to manufacturer’s protocol.

    Article Title: Transcriptomic and functional characterization of megakaryocytic-derived platelet-like particles: impaired aggregation and prominent anti-tumor effects.
    Article Snippet: .. Invading PCa cells on the bottom side of the PET membrane were fixed, stained with Diff Stain kit (IMEB Inc.), and counted. .. Apoptotic activity was measured using the Caspase-Glo 3/7 Assay (Promega) according to the manufacturer’s protocol.9 For dose-response studies 20 000 PCa cells were seeded alone or with PLPs at cell ratios of 1:10, 1:100, 1:500, or 1:1000 into 96 well plates for 24 hours.

    Positron Emission Tomography:

    Article Title: Prostate cancer cell-platelet bidirectional signaling promotes calcium mobilization, invasion and apoptotic resistance via distinct receptor-ligand pairs.
    Article Snippet: .. The PCa cell-platelet coculture was incubated for a total of 48 h. PCa cells invading through Matrigel and residing on the bottom side of the PET membrane were fixed, stained (nuclear and cytoplasm) with Diff Stain kit (IMEB Inc., San Marcos, CA), and counted under light microscope. .. Apoptotic activity was measured using the Caspase-Glo 3/7 Assay (Promega) according to manufacturer’s protocol.

    Article Title: Transcriptomic and functional characterization of megakaryocytic-derived platelet-like particles: impaired aggregation and prominent anti-tumor effects.
    Article Snippet: .. Invading PCa cells on the bottom side of the PET membrane were fixed, stained with Diff Stain kit (IMEB Inc.), and counted. .. Apoptotic activity was measured using the Caspase-Glo 3/7 Assay (Promega) according to the manufacturer’s protocol.9 For dose-response studies 20 000 PCa cells were seeded alone or with PLPs at cell ratios of 1:10, 1:100, 1:500, or 1:1000 into 96 well plates for 24 hours.

    Light Microscopy:

    Article Title: Prostate cancer cell-platelet bidirectional signaling promotes calcium mobilization, invasion and apoptotic resistance via distinct receptor-ligand pairs.
    Article Snippet: .. The PCa cell-platelet coculture was incubated for a total of 48 h. PCa cells invading through Matrigel and residing on the bottom side of the PET membrane were fixed, stained (nuclear and cytoplasm) with Diff Stain kit (IMEB Inc., San Marcos, CA), and counted under light microscope. .. Apoptotic activity was measured using the Caspase-Glo 3/7 Assay (Promega) according to manufacturer’s protocol.

    other:

    Article Title: Impact of oral simvastatin therapy on acute lung injury in mice during pneumococcal pneumonia
    Article Snippet: Differential cell counts were done following cytospin and staining with a Diff-Quick Staining Kit (IMEB Inc.); >300 cells were counted in three separate fields for each mouse.

    Article Title: Combination treatment of chemoresistant cancers
    Article Snippet: Invasive cells were fixed with 4% paraformaldehyde and stained by Diff-Quick staining kits (IMEB Inc, San Marcos, CA) and visualized with an inverted microscope (Nikon).



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    Image Search Results


    RBM23 knockdown inhibits colon cancer cell growth and survival (A) Protein was extracted from the normal colon cell lines CCD-18Co and colon cancer cell lines (HCT116, HT29, LOVO, LS513, DLD-1, COLO205, HCT15) and quantified in equal amounts, and then the protein expression of RBM23 was detected by western blotting. (B) The mRNA expression level of RBM23 was measured by qRT-PCR in the normal colon cell line CCD-18Co and colon cancer cell lines (DLD-1, HCT15, HT29, HCT116). GAPDH was used as an internal control for normalization. (C) Western blot analysis was performed to measure the expression levels of c-Myc in HCT15 cells treated with control siRNA or RBM23 siRNA for 72 h. (D) HCT116 cells were treated with control siRNA or RBM23 siRNA #1, #2, #3 for 72 h. Protein expression levels of various factors were then analyzed by western blotting. (E) The effect of RBM23 deficiency on HCT116, a colon cancer cell line. HCT116 was transfected with 80 nM RBM23 siRNA and control siRNA, and cell viability was examined at 0 and 72 h of treatment. Cell viability was measured by CCK8 assay and optical density was measured by microplate reader at 450 nm. Data were normalized to the control siRNA group at 0 h (set as 100%). (F) The effect of RBM23 knockdown in colon cancer cells, HCT116, on cell proliferation. HCT116 cells were transfected with RBM23 siRNA (100 nM) and incubated for one week, and colonies were stained with Diff Quick solution. The colonies were counted and quantified. (G) Western blotting was performed to detect proteins extracted from HCT116 cells fractionated into nucleus and cytoplasm. Proteins from HCT116 cells transfected with RBM23 siRNA (100 nM) for 72 h were extracted and separated by NE-PER nuclear and cytoplasmic extraction kit into proteins extracted from the nucleus and cytoplasm. The proteins were then detected using western blotting. Student’s t -test: ***P < 0.001

    Journal: Cancer Cell International

    Article Title: Inhibition of RBM23 induces ferroptosis in colon cancer cells via c-Myc regulation

    doi: 10.1186/s12935-026-04262-x

    Figure Lengend Snippet: RBM23 knockdown inhibits colon cancer cell growth and survival (A) Protein was extracted from the normal colon cell lines CCD-18Co and colon cancer cell lines (HCT116, HT29, LOVO, LS513, DLD-1, COLO205, HCT15) and quantified in equal amounts, and then the protein expression of RBM23 was detected by western blotting. (B) The mRNA expression level of RBM23 was measured by qRT-PCR in the normal colon cell line CCD-18Co and colon cancer cell lines (DLD-1, HCT15, HT29, HCT116). GAPDH was used as an internal control for normalization. (C) Western blot analysis was performed to measure the expression levels of c-Myc in HCT15 cells treated with control siRNA or RBM23 siRNA for 72 h. (D) HCT116 cells were treated with control siRNA or RBM23 siRNA #1, #2, #3 for 72 h. Protein expression levels of various factors were then analyzed by western blotting. (E) The effect of RBM23 deficiency on HCT116, a colon cancer cell line. HCT116 was transfected with 80 nM RBM23 siRNA and control siRNA, and cell viability was examined at 0 and 72 h of treatment. Cell viability was measured by CCK8 assay and optical density was measured by microplate reader at 450 nm. Data were normalized to the control siRNA group at 0 h (set as 100%). (F) The effect of RBM23 knockdown in colon cancer cells, HCT116, on cell proliferation. HCT116 cells were transfected with RBM23 siRNA (100 nM) and incubated for one week, and colonies were stained with Diff Quick solution. The colonies were counted and quantified. (G) Western blotting was performed to detect proteins extracted from HCT116 cells fractionated into nucleus and cytoplasm. Proteins from HCT116 cells transfected with RBM23 siRNA (100 nM) for 72 h were extracted and separated by NE-PER nuclear and cytoplasmic extraction kit into proteins extracted from the nucleus and cytoplasm. The proteins were then detected using western blotting. Student’s t -test: ***P < 0.001

    Article Snippet: The plates were then incubated at 37 °C, 5% CO 2 for 1 week and the colonies were fixed and stained using Diff-Quick Kit (Sysmex Corporation, Kobe, Hyogo, Japan) and the number of colonies was counted.

    Techniques: Knockdown, Expressing, Western Blot, Quantitative RT-PCR, Control, Transfection, CCK-8 Assay, Incubation, Staining, Diff-Quik, Extraction