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Yeasen Biotechnology diff-quik stain
Diff Quik Stain, supplied by Yeasen Biotechnology, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/diff+quick+kit/diff+quick+stain+kit/pm35486965-74-6-8
Average 90 stars, based on 1 article reviews
diff-quik stain - by Bioz Stars, 2026-11
90/100 stars

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Related Articles

Staining:

Article Title: BRD4-PRC2 represses transcription of T-helper 2-specific negative regulators during T-cell differentiation.
Article Snippet: Cells in the BAL were counted and stained with Diff-Quick staining kit (Yeasen) and OVA-specific IgE was measured using ELISA kits (eBiosciecne).

Article Title: Fermented lily bulbs by "Jiangshui" probiotics improves lung health in mice.
Article Snippet: Limited application in protecting lung health is attributed to the low levels of active compounds in lily plant bulbs.. This study focused on enhancing the active compounds by fermenting Lilium davidii (Lanzhou Lily) bulbs with Limosilactobacillus fermentum GR-3, isolated from Jiangshui.. Lily fermented bulbs with strain GR-3 (LFB+GR-3) increased the bioavailability of hexadecanoic acid methyl ester, 22-tetrahydroxy-5alpha-cholestan-6-one-3-O-beta-D-allopyranoside, 22-O-(6-deoxy-Alpha-L-mannopyranosyl)-3-O-beta-D-glucopyranosylpregn-5-en-20-one, 1-O-trans-feruloylglycerol, and 3,4 dihydroxybenzoic acid.

Article Title: Down-Regulation of <i>INSR</i> Restores Th17/Treg Immune Balance and Alleviates Airway Hyperviscosity in Asthmatic Mice via Inactivation of STAT3 Pathway
Article Snippet: Background: Allergic asthma (AA) is a prevalent chronic airway inflammation disease.. In this study, this study aims to investigate the biological functions and potential regulatory mechanisms of the insulin receptor (INSR) in the progression of AA.. Methods: BALB/c mice (n = 48) were randomly divided into the following groups: control group, AA group, AA+Lentivirus (Lv)-vector short hairpin RNA (shRNA) group, AA+Lv-vector group, AA+Lv-INSR shRNA group, and AA+Lv-INSR group.

Article Title: Pulmonary Surfactant Homeostasis Dysfunction Mediates Multiwalled Carbon Nanotubes Induced Lung Fibrosis via Elevating Surface Tension.
Article Snippet: Cellular precipitations were stained with Diff-Quik stain (Yeasen, Shanghai, China) according to the manufacturer's recommendations to assess immune cell count.

Article Title: Pulmonary Toxicity Assessment after a Single Intratracheal Inhalation of Chlorhexidine Aerosol in Mice.
Article Snippet: After static drying, the inflammatory cells were stained using the Diff-Quick Stain Kit (Yeasen, Shanghai, China).

Article Title: N-Acetylglucosamine mitigates lung injury and pulmonary fibrosis induced by bleomycin.
Article Snippet: Bronchoalveolar lavage fluid (BALF) was performed using the Diff-Quick Stain Kit (YEASEN, Shanghai, China) according to the manufacturer’s protocol.

Article Title: Carbon nitride nanosheets induce pulmonary surfactant deposition via dysfunction of alveolar secretion and clearance
Article Snippet: Graphitic carbon nitride is a new type of carbon-based nanomaterial with superior properties and great potential for application, which raised great concerns about their environmental and occupational exposure.. Graphitic carbon nitride has been reported to accumulate in the lung potentially, however, the respiratory hazard effect of graphitic carbon nitride is still unknown.. In the present study, we reported that graphitic carbon nitride (g-CN) and its doped variant sulfur doped graphitic carbon nitride (S-g-CN) nanosheets inhalation induced pulmonary inflammation, increased the production of pulmonary surfactant in alveolar type II epithelial cells, accompanied by the upregulation of lipids transporter expression levels in alveolar macrophages to clear excessive pulmonary surfactant in the alveolar.

Article Title: Platycoside E alleviates allergic airway inflammation in obesity-related asthma mouse model.
Article Snippet: Background: Overweight and obesity are related to an increased risk of asthma.. The effect of platycoside E (PE) on obesity-related asthma remains unknown.. Methods: To mimic obesity-related asthma conditions in vivo, C57BL/6 mice were exposed to a high-fat diet (HFD) and challenged with ovalbumin (OVA).

Diff-Quik:

Article Title: BRD4-PRC2 represses transcription of T-helper 2-specific negative regulators during T-cell differentiation.
Article Snippet: Cells in the BAL were counted and stained with Diff-Quick staining kit (Yeasen) and OVA-specific IgE was measured using ELISA kits (eBiosciecne).

Article Title: Fermented lily bulbs by "Jiangshui" probiotics improves lung health in mice.
Article Snippet: Limited application in protecting lung health is attributed to the low levels of active compounds in lily plant bulbs.. This study focused on enhancing the active compounds by fermenting Lilium davidii (Lanzhou Lily) bulbs with Limosilactobacillus fermentum GR-3, isolated from Jiangshui.. Lily fermented bulbs with strain GR-3 (LFB+GR-3) increased the bioavailability of hexadecanoic acid methyl ester, 22-tetrahydroxy-5alpha-cholestan-6-one-3-O-beta-D-allopyranoside, 22-O-(6-deoxy-Alpha-L-mannopyranosyl)-3-O-beta-D-glucopyranosylpregn-5-en-20-one, 1-O-trans-feruloylglycerol, and 3,4 dihydroxybenzoic acid.

Article Title: Down-Regulation of <i>INSR</i> Restores Th17/Treg Immune Balance and Alleviates Airway Hyperviscosity in Asthmatic Mice via Inactivation of STAT3 Pathway
Article Snippet: Background: Allergic asthma (AA) is a prevalent chronic airway inflammation disease.. In this study, this study aims to investigate the biological functions and potential regulatory mechanisms of the insulin receptor (INSR) in the progression of AA.. Methods: BALB/c mice (n = 48) were randomly divided into the following groups: control group, AA group, AA+Lentivirus (Lv)-vector short hairpin RNA (shRNA) group, AA+Lv-vector group, AA+Lv-INSR shRNA group, and AA+Lv-INSR group.

Article Title: Pulmonary Surfactant Homeostasis Dysfunction Mediates Multiwalled Carbon Nanotubes Induced Lung Fibrosis via Elevating Surface Tension.
Article Snippet: Cellular precipitations were stained with Diff-Quik stain (Yeasen, Shanghai, China) according to the manufacturer's recommendations to assess immune cell count.

Article Title: Pulmonary Toxicity Assessment after a Single Intratracheal Inhalation of Chlorhexidine Aerosol in Mice.
Article Snippet: After static drying, the inflammatory cells were stained using the Diff-Quick Stain Kit (Yeasen, Shanghai, China).

Article Title: N-Acetylglucosamine mitigates lung injury and pulmonary fibrosis induced by bleomycin.
Article Snippet: Bronchoalveolar lavage fluid (BALF) was performed using the Diff-Quick Stain Kit (YEASEN, Shanghai, China) according to the manufacturer’s protocol.

Article Title: Carbon nitride nanosheets induce pulmonary surfactant deposition via dysfunction of alveolar secretion and clearance
Article Snippet: Graphitic carbon nitride is a new type of carbon-based nanomaterial with superior properties and great potential for application, which raised great concerns about their environmental and occupational exposure.. Graphitic carbon nitride has been reported to accumulate in the lung potentially, however, the respiratory hazard effect of graphitic carbon nitride is still unknown.. In the present study, we reported that graphitic carbon nitride (g-CN) and its doped variant sulfur doped graphitic carbon nitride (S-g-CN) nanosheets inhalation induced pulmonary inflammation, increased the production of pulmonary surfactant in alveolar type II epithelial cells, accompanied by the upregulation of lipids transporter expression levels in alveolar macrophages to clear excessive pulmonary surfactant in the alveolar.

Article Title: Platycoside E alleviates allergic airway inflammation in obesity-related asthma mouse model.
Article Snippet: Background: Overweight and obesity are related to an increased risk of asthma.. The effect of platycoside E (PE) on obesity-related asthma remains unknown.. Methods: To mimic obesity-related asthma conditions in vivo, C57BL/6 mice were exposed to a high-fat diet (HFD) and challenged with ovalbumin (OVA).

Cell Counting:

Article Title: BRD4-PRC2 represses transcription of T-helper 2-specific negative regulators during T-cell differentiation.
Article Snippet: Cells in the BAL were counted and stained with Diff-Quick staining kit (Yeasen) and OVA-specific IgE was measured using ELISA kits (eBiosciecne).

Article Title: Fermented lily bulbs by "Jiangshui" probiotics improves lung health in mice.
Article Snippet: Limited application in protecting lung health is attributed to the low levels of active compounds in lily plant bulbs.. This study focused on enhancing the active compounds by fermenting Lilium davidii (Lanzhou Lily) bulbs with Limosilactobacillus fermentum GR-3, isolated from Jiangshui.. Lily fermented bulbs with strain GR-3 (LFB+GR-3) increased the bioavailability of hexadecanoic acid methyl ester, 22-tetrahydroxy-5alpha-cholestan-6-one-3-O-beta-D-allopyranoside, 22-O-(6-deoxy-Alpha-L-mannopyranosyl)-3-O-beta-D-glucopyranosylpregn-5-en-20-one, 1-O-trans-feruloylglycerol, and 3,4 dihydroxybenzoic acid.

Article Title: Down-Regulation of <i>INSR</i> Restores Th17/Treg Immune Balance and Alleviates Airway Hyperviscosity in Asthmatic Mice via Inactivation of STAT3 Pathway
Article Snippet: Background: Allergic asthma (AA) is a prevalent chronic airway inflammation disease.. In this study, this study aims to investigate the biological functions and potential regulatory mechanisms of the insulin receptor (INSR) in the progression of AA.. Methods: BALB/c mice (n = 48) were randomly divided into the following groups: control group, AA group, AA+Lentivirus (Lv)-vector short hairpin RNA (shRNA) group, AA+Lv-vector group, AA+Lv-INSR shRNA group, and AA+Lv-INSR group.

Article Title: Pulmonary Surfactant Homeostasis Dysfunction Mediates Multiwalled Carbon Nanotubes Induced Lung Fibrosis via Elevating Surface Tension.
Article Snippet: Cellular precipitations were stained with Diff-Quik stain (Yeasen, Shanghai, China) according to the manufacturer's recommendations to assess immune cell count.

Article Title: Pulmonary Toxicity Assessment after a Single Intratracheal Inhalation of Chlorhexidine Aerosol in Mice.
Article Snippet: After static drying, the inflammatory cells were stained using the Diff-Quick Stain Kit (Yeasen, Shanghai, China).

Article Title: N-Acetylglucosamine mitigates lung injury and pulmonary fibrosis induced by bleomycin.
Article Snippet: Bronchoalveolar lavage fluid (BALF) was performed using the Diff-Quick Stain Kit (YEASEN, Shanghai, China) according to the manufacturer’s protocol.

Article Title: Carbon nitride nanosheets induce pulmonary surfactant deposition via dysfunction of alveolar secretion and clearance
Article Snippet: Graphitic carbon nitride is a new type of carbon-based nanomaterial with superior properties and great potential for application, which raised great concerns about their environmental and occupational exposure.. Graphitic carbon nitride has been reported to accumulate in the lung potentially, however, the respiratory hazard effect of graphitic carbon nitride is still unknown.. In the present study, we reported that graphitic carbon nitride (g-CN) and its doped variant sulfur doped graphitic carbon nitride (S-g-CN) nanosheets inhalation induced pulmonary inflammation, increased the production of pulmonary surfactant in alveolar type II epithelial cells, accompanied by the upregulation of lipids transporter expression levels in alveolar macrophages to clear excessive pulmonary surfactant in the alveolar.

Article Title: Platycoside E alleviates allergic airway inflammation in obesity-related asthma mouse model.
Article Snippet: Background: Overweight and obesity are related to an increased risk of asthma.. The effect of platycoside E (PE) on obesity-related asthma remains unknown.. Methods: To mimic obesity-related asthma conditions in vivo, C57BL/6 mice were exposed to a high-fat diet (HFD) and challenged with ovalbumin (OVA).

Saline:

Article Title: BRD4-PRC2 represses transcription of T-helper 2-specific negative regulators during T-cell differentiation.
Article Snippet: Cells in the BAL were counted and stained with Diff-Quick staining kit (Yeasen) and OVA-specific IgE was measured using ELISA kits (eBiosciecne).

Article Title: Fermented lily bulbs by "Jiangshui" probiotics improves lung health in mice.
Article Snippet: Limited application in protecting lung health is attributed to the low levels of active compounds in lily plant bulbs.. This study focused on enhancing the active compounds by fermenting Lilium davidii (Lanzhou Lily) bulbs with Limosilactobacillus fermentum GR-3, isolated from Jiangshui.. Lily fermented bulbs with strain GR-3 (LFB+GR-3) increased the bioavailability of hexadecanoic acid methyl ester, 22-tetrahydroxy-5alpha-cholestan-6-one-3-O-beta-D-allopyranoside, 22-O-(6-deoxy-Alpha-L-mannopyranosyl)-3-O-beta-D-glucopyranosylpregn-5-en-20-one, 1-O-trans-feruloylglycerol, and 3,4 dihydroxybenzoic acid.

Article Title: Down-Regulation of <i>INSR</i> Restores Th17/Treg Immune Balance and Alleviates Airway Hyperviscosity in Asthmatic Mice via Inactivation of STAT3 Pathway
Article Snippet: Background: Allergic asthma (AA) is a prevalent chronic airway inflammation disease.. In this study, this study aims to investigate the biological functions and potential regulatory mechanisms of the insulin receptor (INSR) in the progression of AA.. Methods: BALB/c mice (n = 48) were randomly divided into the following groups: control group, AA group, AA+Lentivirus (Lv)-vector short hairpin RNA (shRNA) group, AA+Lv-vector group, AA+Lv-INSR shRNA group, and AA+Lv-INSR group.

Article Title: Pulmonary Surfactant Homeostasis Dysfunction Mediates Multiwalled Carbon Nanotubes Induced Lung Fibrosis via Elevating Surface Tension.
Article Snippet: Cellular precipitations were stained with Diff-Quik stain (Yeasen, Shanghai, China) according to the manufacturer's recommendations to assess immune cell count.

Article Title: Pulmonary Toxicity Assessment after a Single Intratracheal Inhalation of Chlorhexidine Aerosol in Mice.
Article Snippet: After static drying, the inflammatory cells were stained using the Diff-Quick Stain Kit (Yeasen, Shanghai, China).

Article Title: N-Acetylglucosamine mitigates lung injury and pulmonary fibrosis induced by bleomycin.
Article Snippet: Bronchoalveolar lavage fluid (BALF) was performed using the Diff-Quick Stain Kit (YEASEN, Shanghai, China) according to the manufacturer’s protocol.

Article Title: Carbon nitride nanosheets induce pulmonary surfactant deposition via dysfunction of alveolar secretion and clearance
Article Snippet: Graphitic carbon nitride is a new type of carbon-based nanomaterial with superior properties and great potential for application, which raised great concerns about their environmental and occupational exposure.. Graphitic carbon nitride has been reported to accumulate in the lung potentially, however, the respiratory hazard effect of graphitic carbon nitride is still unknown.. In the present study, we reported that graphitic carbon nitride (g-CN) and its doped variant sulfur doped graphitic carbon nitride (S-g-CN) nanosheets inhalation induced pulmonary inflammation, increased the production of pulmonary surfactant in alveolar type II epithelial cells, accompanied by the upregulation of lipids transporter expression levels in alveolar macrophages to clear excessive pulmonary surfactant in the alveolar.

Article Title: Platycoside E alleviates allergic airway inflammation in obesity-related asthma mouse model.
Article Snippet: Background: Overweight and obesity are related to an increased risk of asthma.. The effect of platycoside E (PE) on obesity-related asthma remains unknown.. Methods: To mimic obesity-related asthma conditions in vivo, C57BL/6 mice were exposed to a high-fat diet (HFD) and challenged with ovalbumin (OVA).

Microscopy:

Article Title: BRD4-PRC2 represses transcription of T-helper 2-specific negative regulators during T-cell differentiation.
Article Snippet: Cells in the BAL were counted and stained with Diff-Quick staining kit (Yeasen) and OVA-specific IgE was measured using ELISA kits (eBiosciecne).

Article Title: Fermented lily bulbs by "Jiangshui" probiotics improves lung health in mice.
Article Snippet: Limited application in protecting lung health is attributed to the low levels of active compounds in lily plant bulbs.. This study focused on enhancing the active compounds by fermenting Lilium davidii (Lanzhou Lily) bulbs with Limosilactobacillus fermentum GR-3, isolated from Jiangshui.. Lily fermented bulbs with strain GR-3 (LFB+GR-3) increased the bioavailability of hexadecanoic acid methyl ester, 22-tetrahydroxy-5alpha-cholestan-6-one-3-O-beta-D-allopyranoside, 22-O-(6-deoxy-Alpha-L-mannopyranosyl)-3-O-beta-D-glucopyranosylpregn-5-en-20-one, 1-O-trans-feruloylglycerol, and 3,4 dihydroxybenzoic acid.

Article Title: Down-Regulation of <i>INSR</i> Restores Th17/Treg Immune Balance and Alleviates Airway Hyperviscosity in Asthmatic Mice via Inactivation of STAT3 Pathway
Article Snippet: Background: Allergic asthma (AA) is a prevalent chronic airway inflammation disease.. In this study, this study aims to investigate the biological functions and potential regulatory mechanisms of the insulin receptor (INSR) in the progression of AA.. Methods: BALB/c mice (n = 48) were randomly divided into the following groups: control group, AA group, AA+Lentivirus (Lv)-vector short hairpin RNA (shRNA) group, AA+Lv-vector group, AA+Lv-INSR shRNA group, and AA+Lv-INSR group.

Article Title: Pulmonary Surfactant Homeostasis Dysfunction Mediates Multiwalled Carbon Nanotubes Induced Lung Fibrosis via Elevating Surface Tension.
Article Snippet: Cellular precipitations were stained with Diff-Quik stain (Yeasen, Shanghai, China) according to the manufacturer's recommendations to assess immune cell count.

Article Title: Pulmonary Toxicity Assessment after a Single Intratracheal Inhalation of Chlorhexidine Aerosol in Mice.
Article Snippet: After static drying, the inflammatory cells were stained using the Diff-Quick Stain Kit (Yeasen, Shanghai, China).

Article Title: N-Acetylglucosamine mitigates lung injury and pulmonary fibrosis induced by bleomycin.
Article Snippet: Bronchoalveolar lavage fluid (BALF) was performed using the Diff-Quick Stain Kit (YEASEN, Shanghai, China) according to the manufacturer’s protocol.

Article Title: Carbon nitride nanosheets induce pulmonary surfactant deposition via dysfunction of alveolar secretion and clearance
Article Snippet: Graphitic carbon nitride is a new type of carbon-based nanomaterial with superior properties and great potential for application, which raised great concerns about their environmental and occupational exposure.. Graphitic carbon nitride has been reported to accumulate in the lung potentially, however, the respiratory hazard effect of graphitic carbon nitride is still unknown.. In the present study, we reported that graphitic carbon nitride (g-CN) and its doped variant sulfur doped graphitic carbon nitride (S-g-CN) nanosheets inhalation induced pulmonary inflammation, increased the production of pulmonary surfactant in alveolar type II epithelial cells, accompanied by the upregulation of lipids transporter expression levels in alveolar macrophages to clear excessive pulmonary surfactant in the alveolar.

Article Title: Platycoside E alleviates allergic airway inflammation in obesity-related asthma mouse model.
Article Snippet: Background: Overweight and obesity are related to an increased risk of asthma.. The effect of platycoside E (PE) on obesity-related asthma remains unknown.. Methods: To mimic obesity-related asthma conditions in vivo, C57BL/6 mice were exposed to a high-fat diet (HFD) and challenged with ovalbumin (OVA).

Enzyme-linked Immunosorbent Assay:

Article Title: BRD4-PRC2 represses transcription of T-helper 2-specific negative regulators during T-cell differentiation.
Article Snippet: Cells in the BAL were counted and stained with Diff-Quick staining kit (Yeasen) and OVA-specific IgE was measured using ELISA kits (eBiosciecne).

Article Title: Fermented lily bulbs by "Jiangshui" probiotics improves lung health in mice.
Article Snippet: Limited application in protecting lung health is attributed to the low levels of active compounds in lily plant bulbs.. This study focused on enhancing the active compounds by fermenting Lilium davidii (Lanzhou Lily) bulbs with Limosilactobacillus fermentum GR-3, isolated from Jiangshui.. Lily fermented bulbs with strain GR-3 (LFB+GR-3) increased the bioavailability of hexadecanoic acid methyl ester, 22-tetrahydroxy-5alpha-cholestan-6-one-3-O-beta-D-allopyranoside, 22-O-(6-deoxy-Alpha-L-mannopyranosyl)-3-O-beta-D-glucopyranosylpregn-5-en-20-one, 1-O-trans-feruloylglycerol, and 3,4 dihydroxybenzoic acid.

Article Title: Down-Regulation of <i>INSR</i> Restores Th17/Treg Immune Balance and Alleviates Airway Hyperviscosity in Asthmatic Mice via Inactivation of STAT3 Pathway
Article Snippet: Background: Allergic asthma (AA) is a prevalent chronic airway inflammation disease.. In this study, this study aims to investigate the biological functions and potential regulatory mechanisms of the insulin receptor (INSR) in the progression of AA.. Methods: BALB/c mice (n = 48) were randomly divided into the following groups: control group, AA group, AA+Lentivirus (Lv)-vector short hairpin RNA (shRNA) group, AA+Lv-vector group, AA+Lv-INSR shRNA group, and AA+Lv-INSR group.

Article Title: Pulmonary Surfactant Homeostasis Dysfunction Mediates Multiwalled Carbon Nanotubes Induced Lung Fibrosis via Elevating Surface Tension.
Article Snippet: Cellular precipitations were stained with Diff-Quik stain (Yeasen, Shanghai, China) according to the manufacturer's recommendations to assess immune cell count.

Article Title: Pulmonary Toxicity Assessment after a Single Intratracheal Inhalation of Chlorhexidine Aerosol in Mice.
Article Snippet: After static drying, the inflammatory cells were stained using the Diff-Quick Stain Kit (Yeasen, Shanghai, China).

Article Title: N-Acetylglucosamine mitigates lung injury and pulmonary fibrosis induced by bleomycin.
Article Snippet: Bronchoalveolar lavage fluid (BALF) was performed using the Diff-Quick Stain Kit (YEASEN, Shanghai, China) according to the manufacturer’s protocol.

Article Title: Carbon nitride nanosheets induce pulmonary surfactant deposition via dysfunction of alveolar secretion and clearance
Article Snippet: Graphitic carbon nitride is a new type of carbon-based nanomaterial with superior properties and great potential for application, which raised great concerns about their environmental and occupational exposure.. Graphitic carbon nitride has been reported to accumulate in the lung potentially, however, the respiratory hazard effect of graphitic carbon nitride is still unknown.. In the present study, we reported that graphitic carbon nitride (g-CN) and its doped variant sulfur doped graphitic carbon nitride (S-g-CN) nanosheets inhalation induced pulmonary inflammation, increased the production of pulmonary surfactant in alveolar type II epithelial cells, accompanied by the upregulation of lipids transporter expression levels in alveolar macrophages to clear excessive pulmonary surfactant in the alveolar.

Article Title: Platycoside E alleviates allergic airway inflammation in obesity-related asthma mouse model.
Article Snippet: Background: Overweight and obesity are related to an increased risk of asthma.. The effect of platycoside E (PE) on obesity-related asthma remains unknown.. Methods: To mimic obesity-related asthma conditions in vivo, C57BL/6 mice were exposed to a high-fat diet (HFD) and challenged with ovalbumin (OVA).



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RBM23 knockdown inhibits colon cancer cell growth and survival (A) Protein was extracted from the normal colon cell lines CCD-18Co and colon cancer cell lines (HCT116, HT29, LOVO, LS513, DLD-1, COLO205, HCT15) and quantified in equal amounts, and then the protein expression of RBM23 was detected by western blotting. (B) The mRNA expression level of RBM23 was measured by qRT-PCR in the normal colon cell line CCD-18Co and colon cancer cell lines (DLD-1, HCT15, HT29, HCT116). GAPDH was used as an internal control for normalization. (C) Western blot analysis was performed to measure the expression levels of c-Myc in HCT15 cells treated with control siRNA or RBM23 siRNA for 72 h. (D) HCT116 cells were treated with control siRNA or RBM23 siRNA #1, #2, #3 for 72 h. Protein expression levels of various factors were then analyzed by western blotting. (E) The effect of RBM23 deficiency on HCT116, a colon cancer cell line. HCT116 was transfected with 80 nM RBM23 siRNA and control siRNA, and cell viability was examined at 0 and 72 h of treatment. Cell viability was measured by CCK8 assay and optical density was measured by microplate reader at 450 nm. Data were normalized to the control siRNA group at 0 h (set as 100%). (F) The effect of RBM23 knockdown in colon cancer cells, HCT116, on cell proliferation. HCT116 cells were transfected with RBM23 siRNA (100 nM) and incubated for one week, and colonies were stained with <t>Diff</t> <t>Quick</t> solution. The colonies were counted and quantified. (G) Western blotting was performed to detect proteins extracted from HCT116 cells fractionated into nucleus and cytoplasm. Proteins from HCT116 cells transfected with RBM23 siRNA (100 nM) for 72 h were extracted and separated by NE-PER nuclear and cytoplasmic extraction kit into proteins extracted from the nucleus and cytoplasm. The proteins were then detected using western blotting. Student’s t -test: ***P < 0.001
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RBM23 knockdown inhibits colon cancer cell growth and survival (A) Protein was extracted from the normal colon cell lines CCD-18Co and colon cancer cell lines (HCT116, HT29, LOVO, LS513, DLD-1, COLO205, HCT15) and quantified in equal amounts, and then the protein expression of RBM23 was detected by western blotting. (B) The mRNA expression level of RBM23 was measured by qRT-PCR in the normal colon cell line CCD-18Co and colon cancer cell lines (DLD-1, HCT15, HT29, HCT116). GAPDH was used as an internal control for normalization. (C) Western blot analysis was performed to measure the expression levels of c-Myc in HCT15 cells treated with control siRNA or RBM23 siRNA for 72 h. (D) HCT116 cells were treated with control siRNA or RBM23 siRNA #1, #2, #3 for 72 h. Protein expression levels of various factors were then analyzed by western blotting. (E) The effect of RBM23 deficiency on HCT116, a colon cancer cell line. HCT116 was transfected with 80 nM RBM23 siRNA and control siRNA, and cell viability was examined at 0 and 72 h of treatment. Cell viability was measured by CCK8 assay and optical density was measured by microplate reader at 450 nm. Data were normalized to the control siRNA group at 0 h (set as 100%). (F) The effect of RBM23 knockdown in colon cancer cells, HCT116, on cell proliferation. HCT116 cells were transfected with RBM23 siRNA (100 nM) and incubated for one week, and colonies were stained with Diff Quick solution. The colonies were counted and quantified. (G) Western blotting was performed to detect proteins extracted from HCT116 cells fractionated into nucleus and cytoplasm. Proteins from HCT116 cells transfected with RBM23 siRNA (100 nM) for 72 h were extracted and separated by NE-PER nuclear and cytoplasmic extraction kit into proteins extracted from the nucleus and cytoplasm. The proteins were then detected using western blotting. Student’s t -test: ***P < 0.001

Journal: Cancer Cell International

Article Title: Inhibition of RBM23 induces ferroptosis in colon cancer cells via c-Myc regulation

doi: 10.1186/s12935-026-04262-x

Figure Lengend Snippet: RBM23 knockdown inhibits colon cancer cell growth and survival (A) Protein was extracted from the normal colon cell lines CCD-18Co and colon cancer cell lines (HCT116, HT29, LOVO, LS513, DLD-1, COLO205, HCT15) and quantified in equal amounts, and then the protein expression of RBM23 was detected by western blotting. (B) The mRNA expression level of RBM23 was measured by qRT-PCR in the normal colon cell line CCD-18Co and colon cancer cell lines (DLD-1, HCT15, HT29, HCT116). GAPDH was used as an internal control for normalization. (C) Western blot analysis was performed to measure the expression levels of c-Myc in HCT15 cells treated with control siRNA or RBM23 siRNA for 72 h. (D) HCT116 cells were treated with control siRNA or RBM23 siRNA #1, #2, #3 for 72 h. Protein expression levels of various factors were then analyzed by western blotting. (E) The effect of RBM23 deficiency on HCT116, a colon cancer cell line. HCT116 was transfected with 80 nM RBM23 siRNA and control siRNA, and cell viability was examined at 0 and 72 h of treatment. Cell viability was measured by CCK8 assay and optical density was measured by microplate reader at 450 nm. Data were normalized to the control siRNA group at 0 h (set as 100%). (F) The effect of RBM23 knockdown in colon cancer cells, HCT116, on cell proliferation. HCT116 cells were transfected with RBM23 siRNA (100 nM) and incubated for one week, and colonies were stained with Diff Quick solution. The colonies were counted and quantified. (G) Western blotting was performed to detect proteins extracted from HCT116 cells fractionated into nucleus and cytoplasm. Proteins from HCT116 cells transfected with RBM23 siRNA (100 nM) for 72 h were extracted and separated by NE-PER nuclear and cytoplasmic extraction kit into proteins extracted from the nucleus and cytoplasm. The proteins were then detected using western blotting. Student’s t -test: ***P < 0.001

Article Snippet: The plates were then incubated at 37 °C, 5% CO 2 for 1 week and the colonies were fixed and stained using Diff-Quick Kit (Sysmex Corporation, Kobe, Hyogo, Japan) and the number of colonies was counted.

Techniques: Knockdown, Expressing, Western Blot, Quantitative RT-PCR, Control, Transfection, CCK-8 Assay, Incubation, Staining, Diff-Quik, Extraction