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r1050 deposited data microarray  (Zymo Research)


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    Structured Review

    Zymo Research r1050 deposited data microarray
    KEY RESOURCES TABLE
    R1050 Deposited Data Microarray, supplied by Zymo Research, used in various techniques. Bioz Stars score: 99/100, based on 1888 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/deposited+data+microarray+data/Quick-RNA+Microprep+Kit/pmc05847273-658-221-218
    Average 99 stars, based on 1888 article reviews
    r1050 deposited data microarray - by Bioz Stars, 2026-11
    99/100 stars

    Images

    1) Product Images from "The DYT6 Dystonia Protein THAP1 Regulates Myelination Within The Oligodendrocyte Lineage"

    Article Title: The DYT6 Dystonia Protein THAP1 Regulates Myelination Within The Oligodendrocyte Lineage

    Journal: Developmental cell

    doi: 10.1016/j.devcel.2017.06.009

    KEY RESOURCES TABLE
    Figure Legend Snippet: KEY RESOURCES TABLE

    Techniques Used: Recombinant, SYBR Green Assay, Plasmid Preparation, Microarray, Quantitative RT-PCR, Software

    Related Articles

    Isolation:

    Article Title: Evaluation of Interleukin-8 (IL-8) Expression in Human Aortic Endothelial Cells (HAECs) and Antioxidant Activity of Isolated Compounds from the Endemic Hawaiian Plant Pipturus albidus (Māmaki).
    Article Snippet: .. RNA Isolation Total RNA was extracted using the ZYMO Research Quick-RNA MicroPrep kit (Zymo Research Corporation, Tustin, CA, USA) following the manufacturer’s protocol. ..

    Article Title: Circulating miR-21-5p and miR-223-3p as diagnostic biomarkers for rheumatoid arthritis in a sub-Saharan African cohort: a hospital-based case-control study.
    Article Snippet: .. Total RNA including small RNAs was isolated from 200 μL plasma using the Quick-RNA MicroPrep Kit (Zymo Research, Cat. No. R1050) per the manufacturer's protocol: lysis in 400 μL RNA Lysis Buffer, addition of 200 μL 100% ethanol, column purification on Zymo-Spin IC Columns, washing, on-column DNase I digestion for 15 min at room temperature, further washing, and elution in 20 μL RNase-free water with the eluate passed twice through the column. .. RNA quantity and purity were assessed on a NanoDrop One spectrophotometer, with acceptable A260/280 ratios of 1.8–2.1.

    Article Title: Differential requirements for cytokine signaling and local antigen in upper versus lower respiratory tract CD8 + T RM development
    Article Snippet: For bulk RNA sequencing, 72–5,000 cells were sorted directly into RLT lysis buffer (Qiagen) containing 1% β-mercaptoethanol for RNA release and were subsequently flash frozen. .. Total RNA was isolated using the Quick-RNA Microprep kit (Zymo Research). .. Synthesis of cDNA was performed using SMART-seq v4 Ultra Low Input RNA kit (Takara).

    Article Title: Recordanelles for transcriptome-wide single-cell RNA recording in human cells.
    Article Snippet: .. Extraction of RNA from the isolated mitochondria or full cell analysis was extracted using the Quick-RNA Microprep Kit (Zymo) following the manufacturer’s instructions. .. Optionally, for storage in between cells or mitochondrial, pellets were stored in DNA/RNA shield (Zymo).

    Article Title: Differential requirements for cytokine signaling and local antigen in upper versus lower respiratory tract CD8+ TRM development.
    Article Snippet: For bulk RNA sequencing, 72–5,000 cells were sorted directly into RLT lysis buffer (Qiagen) containing 1% β-mercaptoethanol for RNA release and were subsequently flash frozen. .. Total RNA was isolated using the Quick-RNA Microprep kit (Zymo Research). .. Synthesis of cDNA was performed using SMART-seq v4 Ultra Low Input RNA kit (Takara).

    Organoids:

    Article Title: Early Eye Organoids Demonstrate Cadherin and Semaphorin Disruption in PAX6 -Related Aniridia.
    Article Snippet: .. Day 35 organoids were collected and RNA was extracted according to the QuickRNA Microprep Kit (Zymo Research, Irvine, CA, USA). .. A total of 500 ng RNA was used for cDNA synthesis using the iScript cDNA synthesis kit (BioRad, Hercules, CA, USA).

    Clinical Proteomics:

    Article Title: Circulating miR-21-5p and miR-223-3p as diagnostic biomarkers for rheumatoid arthritis in a sub-Saharan African cohort: a hospital-based case-control study.
    Article Snippet: .. Total RNA including small RNAs was isolated from 200 μL plasma using the Quick-RNA MicroPrep Kit (Zymo Research, Cat. No. R1050) per the manufacturer's protocol: lysis in 400 μL RNA Lysis Buffer, addition of 200 μL 100% ethanol, column purification on Zymo-Spin IC Columns, washing, on-column DNase I digestion for 15 min at room temperature, further washing, and elution in 20 μL RNase-free water with the eluate passed twice through the column. .. RNA quantity and purity were assessed on a NanoDrop One spectrophotometer, with acceptable A260/280 ratios of 1.8–2.1.

    Lysis:

    Article Title: Circulating miR-21-5p and miR-223-3p as diagnostic biomarkers for rheumatoid arthritis in a sub-Saharan African cohort: a hospital-based case-control study.
    Article Snippet: .. Total RNA including small RNAs was isolated from 200 μL plasma using the Quick-RNA MicroPrep Kit (Zymo Research, Cat. No. R1050) per the manufacturer's protocol: lysis in 400 μL RNA Lysis Buffer, addition of 200 μL 100% ethanol, column purification on Zymo-Spin IC Columns, washing, on-column DNase I digestion for 15 min at room temperature, further washing, and elution in 20 μL RNase-free water with the eluate passed twice through the column. .. RNA quantity and purity were assessed on a NanoDrop One spectrophotometer, with acceptable A260/280 ratios of 1.8–2.1.

    Purification:

    Article Title: Circulating miR-21-5p and miR-223-3p as diagnostic biomarkers for rheumatoid arthritis in a sub-Saharan African cohort: a hospital-based case-control study.
    Article Snippet: .. Total RNA including small RNAs was isolated from 200 μL plasma using the Quick-RNA MicroPrep Kit (Zymo Research, Cat. No. R1050) per the manufacturer's protocol: lysis in 400 μL RNA Lysis Buffer, addition of 200 μL 100% ethanol, column purification on Zymo-Spin IC Columns, washing, on-column DNase I digestion for 15 min at room temperature, further washing, and elution in 20 μL RNase-free water with the eluate passed twice through the column. .. RNA quantity and purity were assessed on a NanoDrop One spectrophotometer, with acceptable A260/280 ratios of 1.8–2.1.

    Extraction:

    Article Title: Recordanelles for transcriptome-wide single-cell RNA recording in human cells.
    Article Snippet: .. Extraction of RNA from the isolated mitochondria or full cell analysis was extracted using the Quick-RNA Microprep Kit (Zymo) following the manufacturer’s instructions. .. Optionally, for storage in between cells or mitochondrial, pellets were stored in DNA/RNA shield (Zymo).

    Cell Analysis:

    Article Title: Recordanelles for transcriptome-wide single-cell RNA recording in human cells.
    Article Snippet: .. Extraction of RNA from the isolated mitochondria or full cell analysis was extracted using the Quick-RNA Microprep Kit (Zymo) following the manufacturer’s instructions. .. Optionally, for storage in between cells or mitochondrial, pellets were stored in DNA/RNA shield (Zymo).



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