dy417 deposited data microarray dataset peritoneal macrophages (R&D Systems)
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dy417 deposited data microarray dataset peritoneal macrophages
Dy417 Deposited Data Microarray Dataset Peritoneal Macrophages, supplied by R&D Systems, used in various techniques. Bioz Stars score: 96/100, based on 372 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/deposited+data+microarray+data/Mouse+IL-10+DuoSet+ELISA/pm32049012-206-216-213
Average 96 stars, based on 372 article reviews
Dy417 Deposited Data Microarray Dataset Peritoneal Macrophages, supplied by R&D Systems, used in various techniques. Bioz Stars score: 96/100, based on 372 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/deposited+data+microarray+data/Mouse+IL-10+DuoSet+ELISA/pm32049012-206-216-213
Average 96 stars, based on 372 article reviews
dy417 deposited data microarray dataset peritoneal macrophages - by Bioz Stars,
2026-11
96/100 stars
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Incubation:Article Title: Myeloid-derived interleukin-10 induced by thrombospondin-1 mediates host defense and regulates inflammation during acute bacterial lung infection Article Snippet: To assess the role of TSP-1 in IL-10 production, bone marrow CD11b + Ly6G + Ly6C + cells were incubated with recombinant TSP-1 (0, 5, or 15 μg/mL) at 37°C with 5% CO 2 for 24 h. Then, cells were washed with sterile RPMI media and stimulated with bacterial LPS (100 ng/mL) at 37°C with 5% CO 2 for 4 h. In parallel, CD11b + Ly6G + Ly6C + cells were differentiated from WT or Thbs1 −/− mice and stimulated with bacterial LPS (100 ng/mL) for 4 h. For each set of experiments, the supernatant was collected, and IL-10 production was measured by ELISA (DuoSet ELISA Mouse IL-10, R&D Systems, DY417). .. To assess the role of PPARγ in IL-10 production, bone marrow CD11b + Ly6G + Ly6C + cells were incubated with recombinant TSP-1 (0 or 15 μg/mL) at 37°C with 5% CO 2 for 24 h. Then cells were then washed with sterile RPMI media and treated with vehicle (DMSO), the PPARγ agonist GW1929 (100 μM), or the PPARγ antagonist GW9662 (10 μM) for 1 h. All groups were stimulated with bacterial LPS (100 ng/mL) at 37°C with 5% CO 2 for 4 h. The supernatant was collected for Article Title: Myeloid-derived interleukin-10 induced by thrombospondin-1 mediates host defense and regulates inflammation during acute bacterial lung infection. Article Snippet: To assess the role of TSP-1 in IL-10 production, bone marrow CD11b+Ly6G+Ly6C+ cells were incubated with recombinant TSP-1 (0, 5, or 15 μg/mL) at 37°C with 5% CO2 for 24 h. Then, cells were washed with sterile RPMI media and stimulated with bacterial LPS (100 ng/mL) at 37°C with 5% CO2 for 4 h. In parallel, CD11b+Ly6G+Ly6C+ cells were differentiated from WT or Thbs1−/− mice and stimulated with bacterial LPS (100 ng/mL) for 4 h. For each set of experiments, the supernatant was collected, and IL-10 production was measured by ELISA (DuoSet ELISA Mouse IL-10, R&D Systems, DY417). .. To assess the role of PPARγ in IL-10 production, bone marrow CD11b+Ly6G+Ly6C+ cells were incubated with recombinant TSP-1 (0 or 15 μg/mL) at 37°C with 5% CO2 for 24 h. Then cells were then washed with sterile RPMI media and treated with vehicle (DMSO), the PPARγ agonist GW1929 (100 μM), or the PPARγ antagonist GW9662 (10 μM) for 1 h. All groups were stimulated with bacterial LPS (100 ng/mL) at 37°C with 5% CO2 for 4 Month XXXX Volume 0 Issue 0 10.1128/iai.00614-2513 D ow nl oa de d fr om h ttp s: //j ou rn al s. as m .o rg /jo ur na l/i ai o n 26 F eb ru ar y 20 26 b y 24 00 :1 a0 0: 4b ab :4 25 3: 45 cd :7 c9 8: fa 0a :f 53 9. h. The supernatant was collected for Recombinant:Article Title: Myeloid-derived interleukin-10 induced by thrombospondin-1 mediates host defense and regulates inflammation during acute bacterial lung infection Article Snippet: To assess the role of TSP-1 in IL-10 production, bone marrow CD11b + Ly6G + Ly6C + cells were incubated with recombinant TSP-1 (0, 5, or 15 μg/mL) at 37°C with 5% CO 2 for 24 h. Then, cells were washed with sterile RPMI media and stimulated with bacterial LPS (100 ng/mL) at 37°C with 5% CO 2 for 4 h. In parallel, CD11b + Ly6G + Ly6C + cells were differentiated from WT or Thbs1 −/− mice and stimulated with bacterial LPS (100 ng/mL) for 4 h. For each set of experiments, the supernatant was collected, and IL-10 production was measured by ELISA (DuoSet ELISA Mouse IL-10, R&D Systems, DY417). .. To assess the role of PPARγ in IL-10 production, bone marrow CD11b + Ly6G + Ly6C + cells were incubated with recombinant TSP-1 (0 or 15 μg/mL) at 37°C with 5% CO 2 for 24 h. Then cells were then washed with sterile RPMI media and treated with vehicle (DMSO), the PPARγ agonist GW1929 (100 μM), or the PPARγ antagonist GW9662 (10 μM) for 1 h. All groups were stimulated with bacterial LPS (100 ng/mL) at 37°C with 5% CO 2 for 4 h. The supernatant was collected for Article Title: Myeloid-derived interleukin-10 induced by thrombospondin-1 mediates host defense and regulates inflammation during acute bacterial lung infection. Article Snippet: To assess the role of TSP-1 in IL-10 production, bone marrow CD11b+Ly6G+Ly6C+ cells were incubated with recombinant TSP-1 (0, 5, or 15 μg/mL) at 37°C with 5% CO2 for 24 h. Then, cells were washed with sterile RPMI media and stimulated with bacterial LPS (100 ng/mL) at 37°C with 5% CO2 for 4 h. In parallel, CD11b+Ly6G+Ly6C+ cells were differentiated from WT or Thbs1−/− mice and stimulated with bacterial LPS (100 ng/mL) for 4 h. For each set of experiments, the supernatant was collected, and IL-10 production was measured by ELISA (DuoSet ELISA Mouse IL-10, R&D Systems, DY417). .. To assess the role of PPARγ in IL-10 production, bone marrow CD11b+Ly6G+Ly6C+ cells were incubated with recombinant TSP-1 (0 or 15 μg/mL) at 37°C with 5% CO2 for 24 h. Then cells were then washed with sterile RPMI media and treated with vehicle (DMSO), the PPARγ agonist GW1929 (100 μM), or the PPARγ antagonist GW9662 (10 μM) for 1 h. All groups were stimulated with bacterial LPS (100 ng/mL) at 37°C with 5% CO2 for 4 Month XXXX Volume 0 Issue 0 10.1128/iai.00614-2513 D ow nl oa de d fr om h ttp s: //j ou rn al s. as m .o rg /jo ur na l/i ai o n 26 F eb ru ar y 20 26 b y 24 00 :1 a0 0: 4b ab :4 25 3: 45 cd :7 c9 8: fa 0a :f 53 9. h. The supernatant was collected for Article Title: ADSC-derived exosomes deliver cerium oxide nanoparticles to promote diabetic wound healing via anti-inflammatory and pro-angiogenic effects. Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-CD63 antibody Abcam Cat# ab134045; RRID: AB_2800495 Anti-CD81 antibody Abcam Cat# ab79559; RRID: AB_1603682 Anti-CD31 antibody Invitrogen Cat# MA5-37858; RRID: AB_2897778 Chemicals, peptides, and recombinant proteins Cerium(III) nitrate hexahydrate Sigma-Aldrich 238538-5G Ammonium hydroxide Sigma-Aldrich 221228-500 ML Ethanol Sigma-Aldrich 459836-1L Uranyl acetate (2%) Electron Microscopy Sciences 22400–2 Rhodamine B (Rho) Thermo Fisher Scientific R6626 DCFH-DA probe Sigma-Aldrich R6626 DAPI (4 ′ ,6-diamidino-2-phenylindole) Thermo Fisher Scientific D1306 Streptozotocin Sigma-Aldrich S0130-50 MG Paraformaldehyde (4%) Sigma-Aldrich 158127-500G CCK-8 assay kit Beyotime C0037 ELISA kits(IFN-γ) R&D Systems Sterility:Article Title: Myeloid-derived interleukin-10 induced by thrombospondin-1 mediates host defense and regulates inflammation during acute bacterial lung infection Article Snippet: To assess the role of TSP-1 in IL-10 production, bone marrow CD11b + Ly6G + Ly6C + cells were incubated with recombinant TSP-1 (0, 5, or 15 μg/mL) at 37°C with 5% CO 2 for 24 h. Then, cells were washed with sterile RPMI media and stimulated with bacterial LPS (100 ng/mL) at 37°C with 5% CO 2 for 4 h. In parallel, CD11b + Ly6G + Ly6C + cells were differentiated from WT or Thbs1 −/− mice and stimulated with bacterial LPS (100 ng/mL) for 4 h. For each set of experiments, the supernatant was collected, and IL-10 production was measured by ELISA (DuoSet ELISA Mouse IL-10, R&D Systems, DY417). .. To assess the role of PPARγ in IL-10 production, bone marrow CD11b + Ly6G + Ly6C + cells were incubated with recombinant TSP-1 (0 or 15 μg/mL) at 37°C with 5% CO 2 for 24 h. Then cells were then washed with sterile RPMI media and treated with vehicle (DMSO), the PPARγ agonist GW1929 (100 μM), or the PPARγ antagonist GW9662 (10 μM) for 1 h. All groups were stimulated with bacterial LPS (100 ng/mL) at 37°C with 5% CO 2 for 4 h. The supernatant was collected for Article Title: Myeloid-derived interleukin-10 induced by thrombospondin-1 mediates host defense and regulates inflammation during acute bacterial lung infection. Article Snippet: To assess the role of TSP-1 in IL-10 production, bone marrow CD11b+Ly6G+Ly6C+ cells were incubated with recombinant TSP-1 (0, 5, or 15 μg/mL) at 37°C with 5% CO2 for 24 h. Then, cells were washed with sterile RPMI media and stimulated with bacterial LPS (100 ng/mL) at 37°C with 5% CO2 for 4 h. In parallel, CD11b+Ly6G+Ly6C+ cells were differentiated from WT or Thbs1−/− mice and stimulated with bacterial LPS (100 ng/mL) for 4 h. For each set of experiments, the supernatant was collected, and IL-10 production was measured by ELISA (DuoSet ELISA Mouse IL-10, R&D Systems, DY417). .. To assess the role of PPARγ in IL-10 production, bone marrow CD11b+Ly6G+Ly6C+ cells were incubated with recombinant TSP-1 (0 or 15 μg/mL) at 37°C with 5% CO2 for 24 h. Then cells were then washed with sterile RPMI media and treated with vehicle (DMSO), the PPARγ agonist GW1929 (100 μM), or the PPARγ antagonist GW9662 (10 μM) for 1 h. All groups were stimulated with bacterial LPS (100 ng/mL) at 37°C with 5% CO2 for 4 Month XXXX Volume 0 Issue 0 10.1128/iai.00614-2513 D ow nl oa de d fr om h ttp s: //j ou rn al s. as m .o rg /jo ur na l/i ai o n 26 F eb ru ar y 20 26 b y 24 00 :1 a0 0: 4b ab :4 25 3: 45 cd :7 c9 8: fa 0a :f 53 9. h. The supernatant was collected for Enzyme-linked Immunosorbent Assay:Article Title: Myeloid-derived interleukin-10 induced by thrombospondin-1 mediates host defense and regulates inflammation during acute bacterial lung infection Article Snippet: To assess the role of TSP-1 in IL-10 production, bone marrow CD11b + Ly6G + Ly6C + cells were incubated with recombinant TSP-1 (0, 5, or 15 μg/mL) at 37°C with 5% CO 2 for 24 h. Then, cells were washed with sterile RPMI media and stimulated with bacterial LPS (100 ng/mL) at 37°C with 5% CO 2 for 4 h. In parallel, CD11b + Ly6G + Ly6C + cells were differentiated from WT or Thbs1 −/− mice and stimulated with bacterial LPS (100 ng/mL) for 4 h. For each set of experiments, the supernatant was collected, and IL-10 production was measured by ELISA (DuoSet ELISA Mouse IL-10, R&D Systems, DY417). .. To assess the role of PPARγ in IL-10 production, bone marrow CD11b + Ly6G + Ly6C + cells were incubated with recombinant TSP-1 (0 or 15 μg/mL) at 37°C with 5% CO 2 for 24 h. Then cells were then washed with sterile RPMI media and treated with vehicle (DMSO), the PPARγ agonist GW1929 (100 μM), or the PPARγ antagonist GW9662 (10 μM) for 1 h. All groups were stimulated with bacterial LPS (100 ng/mL) at 37°C with 5% CO 2 for 4 h. The supernatant was collected for Article Title: cGAS-STING dependent type I IFN reduces Leptospira interrogans renal colonization in mice. Article Snippet: Leptospira interrogans serovar Copenhageni strain Fiocruz L1-130 and Leptospira biflexa serovar Patoc strain Patoc 1 were grown in Hornsby-Alt-Nally (HAN) media [67]. .. Cytokines and chemokines were quantified in the cell culture supernatants using mouse ELISA kit for TNF (Invitrogen #88-7324-88), IFNβ (R&D Systems #DY8234), Article Title: Myeloid-derived interleukin-10 induced by thrombospondin-1 mediates host defense and regulates inflammation during acute bacterial lung infection. Article Snippet: To assess the role of TSP-1 in IL-10 production, bone marrow CD11b+Ly6G+Ly6C+ cells were incubated with recombinant TSP-1 (0, 5, or 15 μg/mL) at 37°C with 5% CO2 for 24 h. Then, cells were washed with sterile RPMI media and stimulated with bacterial LPS (100 ng/mL) at 37°C with 5% CO2 for 4 h. In parallel, CD11b+Ly6G+Ly6C+ cells were differentiated from WT or Thbs1−/− mice and stimulated with bacterial LPS (100 ng/mL) for 4 h. For each set of experiments, the supernatant was collected, and IL-10 production was measured by ELISA (DuoSet ELISA Mouse IL-10, R&D Systems, DY417). .. To assess the role of PPARγ in IL-10 production, bone marrow CD11b+Ly6G+Ly6C+ cells were incubated with recombinant TSP-1 (0 or 15 μg/mL) at 37°C with 5% CO2 for 24 h. Then cells were then washed with sterile RPMI media and treated with vehicle (DMSO), the PPARγ agonist GW1929 (100 μM), or the PPARγ antagonist GW9662 (10 μM) for 1 h. All groups were stimulated with bacterial LPS (100 ng/mL) at 37°C with 5% CO2 for 4 Month XXXX Volume 0 Issue 0 10.1128/iai.00614-2513 D ow nl oa de d fr om h ttp s: //j ou rn al s. as m .o rg /jo ur na l/i ai o n 26 F eb ru ar y 20 26 b y 24 00 :1 a0 0: 4b ab :4 25 3: 45 cd :7 c9 8: fa 0a :f 53 9. h. The supernatant was collected for Article Title: cGAS-STING dependent type I IFN reduces Leptospira interrogans renal colonization in mice Article Snippet: For transfection of leptospiral DNA, 500 ng of DNA isolated from L. interrogans Fiocruz L1-130 cultures was incubated with Xfect transfection reagent (Takara Bio #631317) for 10 min and BMDMs were treated with the mixture for 6 h. After respective stimulations, supernatants were collected for quantification of cytokines and cells were preserved in RNAlater Stabilization Solution (Invitrogen #AM7020) for RNA isolation using RNeasy Mini kit (Qiagen #74106) as per the manufacturers’ instruction. .. Cytokines and chemokines were quantified in the cell culture supernatants using mouse ELISA kit for TNF (Invitrogen #88-7324-88), IFNβ (R&D Systems #DY8234), Article Title: Structure matters: commensal Phocaeicola vulgatus lipopolysaccharide induces attenuated microglial activation and preserves neuronal integrity Article Snippet: Cytokine levels (pg/mL) in BV2 and HMC3 supernatants were measured following E. coli or P. vulgatus LPS stimulation using the ultrasensitive ELLA-Simple Plex platform (Protein Simple, Bio-Techne, San José, CA, United States), with a commercially available microfluidic cartridge for mouse (ST01C-MP-004435) and for human (ST01A-PS-003229) IL-1β, IL-6, and TNF-α detection. .. IL-10 was quantified using Article Title: Mincle-dependent Th17 adjuvanticity requires TNFR1 signaling in myeloid cells Article Snippet: .. For this purpose, the following ELISA kits were used according to the manufacturer’s instructions: IFNγ (R&D Systems, DY485), IL-17 (R&D Systems, DY421), Article Title: ADSC-derived exosomes deliver cerium oxide nanoparticles to promote diabetic wound healing via anti-inflammatory and pro-angiogenic effects. Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-CD63 antibody Abcam Cat# ab134045; RRID: AB_2800495 Anti-CD81 antibody Abcam Cat# ab79559; RRID: AB_1603682 Anti-CD31 antibody Invitrogen Cat# MA5-37858; RRID: AB_2897778 Chemicals, peptides, and recombinant proteins Cerium(III) nitrate hexahydrate Sigma-Aldrich 238538-5G Ammonium hydroxide Sigma-Aldrich 221228-500 ML Ethanol Sigma-Aldrich 459836-1L Uranyl acetate (2%) Electron Microscopy Sciences 22400–2 Rhodamine B (Rho) Thermo Fisher Scientific R6626 DCFH-DA probe Sigma-Aldrich R6626 DAPI (4 ′ ,6-diamidino-2-phenylindole) Thermo Fisher Scientific D1306 Streptozotocin Sigma-Aldrich S0130-50 MG Paraformaldehyde (4%) Sigma-Aldrich 158127-500G CCK-8 assay kit Beyotime C0037 ELISA kits(IFN-γ) R&D Systems Cell Culture:Article Title: cGAS-STING dependent type I IFN reduces Leptospira interrogans renal colonization in mice. Article Snippet: Leptospira interrogans serovar Copenhageni strain Fiocruz L1-130 and Leptospira biflexa serovar Patoc strain Patoc 1 were grown in Hornsby-Alt-Nally (HAN) media [67]. .. Cytokines and chemokines were quantified in the cell culture supernatants using mouse ELISA kit for TNF (Invitrogen #88-7324-88), IFNβ (R&D Systems #DY8234), Article Title: cGAS-STING dependent type I IFN reduces Leptospira interrogans renal colonization in mice Article Snippet: For transfection of leptospiral DNA, 500 ng of DNA isolated from L. interrogans Fiocruz L1-130 cultures was incubated with Xfect transfection reagent (Takara Bio #631317) for 10 min and BMDMs were treated with the mixture for 6 h. After respective stimulations, supernatants were collected for quantification of cytokines and cells were preserved in RNAlater Stabilization Solution (Invitrogen #AM7020) for RNA isolation using RNeasy Mini kit (Qiagen #74106) as per the manufacturers’ instruction. .. Cytokines and chemokines were quantified in the cell culture supernatants using mouse ELISA kit for TNF (Invitrogen #88-7324-88), IFNβ (R&D Systems #DY8234), Electron Microscopy:Article Title: ADSC-derived exosomes deliver cerium oxide nanoparticles to promote diabetic wound healing via anti-inflammatory and pro-angiogenic effects. Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-CD63 antibody Abcam Cat# ab134045; RRID: AB_2800495 Anti-CD81 antibody Abcam Cat# ab79559; RRID: AB_1603682 Anti-CD31 antibody Invitrogen Cat# MA5-37858; RRID: AB_2897778 Chemicals, peptides, and recombinant proteins Cerium(III) nitrate hexahydrate Sigma-Aldrich 238538-5G Ammonium hydroxide Sigma-Aldrich 221228-500 ML Ethanol Sigma-Aldrich 459836-1L Uranyl acetate (2%) Electron Microscopy Sciences 22400–2 Rhodamine B (Rho) Thermo Fisher Scientific R6626 DCFH-DA probe Sigma-Aldrich R6626 DAPI (4 ′ ,6-diamidino-2-phenylindole) Thermo Fisher Scientific D1306 Streptozotocin Sigma-Aldrich S0130-50 MG Paraformaldehyde (4%) Sigma-Aldrich 158127-500G CCK-8 assay kit Beyotime C0037 ELISA kits(IFN-γ) R&D Systems CCK-8 Assay:Article Title: ADSC-derived exosomes deliver cerium oxide nanoparticles to promote diabetic wound healing via anti-inflammatory and pro-angiogenic effects. Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-CD63 antibody Abcam Cat# ab134045; RRID: AB_2800495 Anti-CD81 antibody Abcam Cat# ab79559; RRID: AB_1603682 Anti-CD31 antibody Invitrogen Cat# MA5-37858; RRID: AB_2897778 Chemicals, peptides, and recombinant proteins Cerium(III) nitrate hexahydrate Sigma-Aldrich 238538-5G Ammonium hydroxide Sigma-Aldrich 221228-500 ML Ethanol Sigma-Aldrich 459836-1L Uranyl acetate (2%) Electron Microscopy Sciences 22400–2 Rhodamine B (Rho) Thermo Fisher Scientific R6626 DCFH-DA probe Sigma-Aldrich R6626 DAPI (4 ′ ,6-diamidino-2-phenylindole) Thermo Fisher Scientific D1306 Streptozotocin Sigma-Aldrich S0130-50 MG Paraformaldehyde (4%) Sigma-Aldrich 158127-500G CCK-8 assay kit Beyotime C0037 ELISA kits(IFN-γ) R&D Systems Modification:Article Title: ADSC-derived exosomes deliver cerium oxide nanoparticles to promote diabetic wound healing via anti-inflammatory and pro-angiogenic effects. Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-CD63 antibody Abcam Cat# ab134045; RRID: AB_2800495 Anti-CD81 antibody Abcam Cat# ab79559; RRID: AB_1603682 Anti-CD31 antibody Invitrogen Cat# MA5-37858; RRID: AB_2897778 Chemicals, peptides, and recombinant proteins Cerium(III) nitrate hexahydrate Sigma-Aldrich 238538-5G Ammonium hydroxide Sigma-Aldrich 221228-500 ML Ethanol Sigma-Aldrich 459836-1L Uranyl acetate (2%) Electron Microscopy Sciences 22400–2 Rhodamine B (Rho) Thermo Fisher Scientific R6626 DCFH-DA probe Sigma-Aldrich R6626 DAPI (4 ′ ,6-diamidino-2-phenylindole) Thermo Fisher Scientific D1306 Streptozotocin Sigma-Aldrich S0130-50 MG Paraformaldehyde (4%) Sigma-Aldrich 158127-500G CCK-8 assay kit Beyotime C0037 ELISA kits(IFN-γ) R&D Systems Software:Article Title: ADSC-derived exosomes deliver cerium oxide nanoparticles to promote diabetic wound healing via anti-inflammatory and pro-angiogenic effects. Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-CD63 antibody Abcam Cat# ab134045; RRID: AB_2800495 Anti-CD81 antibody Abcam Cat# ab79559; RRID: AB_1603682 Anti-CD31 antibody Invitrogen Cat# MA5-37858; RRID: AB_2897778 Chemicals, peptides, and recombinant proteins Cerium(III) nitrate hexahydrate Sigma-Aldrich 238538-5G Ammonium hydroxide Sigma-Aldrich 221228-500 ML Ethanol Sigma-Aldrich 459836-1L Uranyl acetate (2%) Electron Microscopy Sciences 22400–2 Rhodamine B (Rho) Thermo Fisher Scientific R6626 DCFH-DA probe Sigma-Aldrich R6626 DAPI (4 ′ ,6-diamidino-2-phenylindole) Thermo Fisher Scientific D1306 Streptozotocin Sigma-Aldrich S0130-50 MG Paraformaldehyde (4%) Sigma-Aldrich 158127-500G CCK-8 assay kit Beyotime C0037 ELISA kits(IFN-γ) R&D Systems |