Review



10004d deposited data raw  (Thermo Fisher)


Bioz Verified Symbol Thermo Fisher is a verified supplier
Bioz Manufacturer Symbol Thermo Fisher manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 99

    Structured Review

    Thermo Fisher 10004d deposited data raw
    10004d Deposited Data Raw, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 7125 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/deposited+data+microarray+data/Dynabeads+Protein+G+for+Immunoprecipitation/pm31509748-187-63-61
    Average 99 stars, based on 7125 article reviews
    10004d deposited data raw - by Bioz Stars, 2026-11
    99/100 stars

    Images

    Related Articles

    other:

    Article Title: The endothelial-enriched lncRNA LINC00607 mediates angiogenic function
    Article Snippet: The Proliferation Module of the Incucyte S3 Live Cell Analysis System (Essen Bioscience) was used to monitor and analyze the cells.

    Article Title: CPEB1-dependent disruption of the mRNA translation program in oocytes during maternal aging
    Article Snippet: To prepare samples for the IP, the appropriate volume (80 μl per sample) of DynabeadsTM Protein G (Invitrogen, 10004D) was washed two times in 250 μl homogenization buffer (HB: 50 mM Tris–HCl pH 7.4, 100 mM KCl, 12 mM MgCl 2 and 1% v/v NP-40) on a rotor at 4 °C for 5 min per wash. Two additional washes were performed with 250 μl supplemented HB (sHB: HB supplemented with 1 mM DTT (sigma 646563), 1× HALT protease inhibitor (Fisher P178441), 200 units/ml RNaseOUT, 200 units/ml RNasin (Promega N2115), 100 μg/ml CHX) on a rotor at 4 °C for 5 min per wash.

    Article Title: Drug-induced eRF1 degradation promotes readthrough and reveals a new branch of ribosome quality control
    Article Snippet: The rest of the lysate was combined with 6 μg eRF1 antibody (Santa Cruz Biotechnology, Cat# sc-365686) coupled to 0.75 mg DynabeadsTM Protein G (Invitrogen, Cat# 10004D) for 1 h at 4°C under constant rotation.

    Immunoprecipitation:

    Article Title: Integrated multi-omics reveals anaplerotic rewiring in methylmalonyl-CoA mutase deficiency
    Article Snippet: At 48 h after transfection, cells were crosslinked using 0.5% paraformaldehyde (PFA, Sigma-Aldrich) in PBS (Gibco) for 10 min at RT, the reaction was quenched with 1.25 M glycine/PBS (Sigma-Aldrich) for 10 min at 4 °C, cells were centrifuged for 5 min at 2,000 g at 4 °C and the pellet resuspended in lysis buffer (1% Nonidet P-40, 0.5% deoxycholine, 150 mM NaCl and 50 mM Tris-HCl, pH 7.5; all Sigma-Aldrich). .. Pre-cleared cell extracts were immunoprecipitated with anti-FLAG M2 (F3165, Sigma-Aldrich), anti-MMUT (ab67869, Abcam) or anti-MMAB (HPA039017, Sigma-Aldrich) using Dynabeads Protein G (Thermo Fisher Scientific) according to the manufacturer’s instructions. ..

    Chromatin Immunoprecipitation:

    Article Title: An atlas of lamina-associated chromatin across twelve human cell types reveals an intermediate chromatin subtype
    Article Snippet: .. For ChIP, 30μL protein G magnetic beads (per ChIP sample; Dynal) were washed 3 times in blocking buffer (0.5% BSA in PBS); beads were resuspended in 250μL blocking buffer and 2μg antibody (Lamin B1: ab16048 [Abcam]; H3K9me2: ab1220 [Abcam]) and rotated at 4°C for at least 6 h. Crude nuclei were isolated from frozen crosslinked cells as follows: cell pellet (from 10cm plate) was resuspended in 10mL cold Lysis Buffer 1 (50mM HEPES-KOH pH7.5, 140mM NaCl, 1mM EDTA, 10% Glycerol, 0.5% NP-40, 0.25% Triton X-100, and protease inhibitors) and rotated at 4°C for 10 min, followed by centrifugation (250× g , 5 min at room temperature). ..

    Article Title: T-REX17 is a transiently expressed non-coding RNA essential for human endoderm formation
    Article Snippet: .. The next day, 50 μl of Dynabeads Protein G (Thermo Fisher Scientific, 10004D) /IP were washed twice with ice cold ChIP Dilution Buffer and then added to each IPs. ..

    Magnetic Beads:

    Article Title: An atlas of lamina-associated chromatin across twelve human cell types reveals an intermediate chromatin subtype
    Article Snippet: .. For ChIP, 30μL protein G magnetic beads (per ChIP sample; Dynal) were washed 3 times in blocking buffer (0.5% BSA in PBS); beads were resuspended in 250μL blocking buffer and 2μg antibody (Lamin B1: ab16048 [Abcam]; H3K9me2: ab1220 [Abcam]) and rotated at 4°C for at least 6 h. Crude nuclei were isolated from frozen crosslinked cells as follows: cell pellet (from 10cm plate) was resuspended in 10mL cold Lysis Buffer 1 (50mM HEPES-KOH pH7.5, 140mM NaCl, 1mM EDTA, 10% Glycerol, 0.5% NP-40, 0.25% Triton X-100, and protease inhibitors) and rotated at 4°C for 10 min, followed by centrifugation (250× g , 5 min at room temperature). ..

    Blocking Assay:

    Article Title: An atlas of lamina-associated chromatin across twelve human cell types reveals an intermediate chromatin subtype
    Article Snippet: .. For ChIP, 30μL protein G magnetic beads (per ChIP sample; Dynal) were washed 3 times in blocking buffer (0.5% BSA in PBS); beads were resuspended in 250μL blocking buffer and 2μg antibody (Lamin B1: ab16048 [Abcam]; H3K9me2: ab1220 [Abcam]) and rotated at 4°C for at least 6 h. Crude nuclei were isolated from frozen crosslinked cells as follows: cell pellet (from 10cm plate) was resuspended in 10mL cold Lysis Buffer 1 (50mM HEPES-KOH pH7.5, 140mM NaCl, 1mM EDTA, 10% Glycerol, 0.5% NP-40, 0.25% Triton X-100, and protease inhibitors) and rotated at 4°C for 10 min, followed by centrifugation (250× g , 5 min at room temperature). ..

    Isolation:

    Article Title: An atlas of lamina-associated chromatin across twelve human cell types reveals an intermediate chromatin subtype
    Article Snippet: .. For ChIP, 30μL protein G magnetic beads (per ChIP sample; Dynal) were washed 3 times in blocking buffer (0.5% BSA in PBS); beads were resuspended in 250μL blocking buffer and 2μg antibody (Lamin B1: ab16048 [Abcam]; H3K9me2: ab1220 [Abcam]) and rotated at 4°C for at least 6 h. Crude nuclei were isolated from frozen crosslinked cells as follows: cell pellet (from 10cm plate) was resuspended in 10mL cold Lysis Buffer 1 (50mM HEPES-KOH pH7.5, 140mM NaCl, 1mM EDTA, 10% Glycerol, 0.5% NP-40, 0.25% Triton X-100, and protease inhibitors) and rotated at 4°C for 10 min, followed by centrifugation (250× g , 5 min at room temperature). ..

    Lysis:

    Article Title: An atlas of lamina-associated chromatin across twelve human cell types reveals an intermediate chromatin subtype
    Article Snippet: .. For ChIP, 30μL protein G magnetic beads (per ChIP sample; Dynal) were washed 3 times in blocking buffer (0.5% BSA in PBS); beads were resuspended in 250μL blocking buffer and 2μg antibody (Lamin B1: ab16048 [Abcam]; H3K9me2: ab1220 [Abcam]) and rotated at 4°C for at least 6 h. Crude nuclei were isolated from frozen crosslinked cells as follows: cell pellet (from 10cm plate) was resuspended in 10mL cold Lysis Buffer 1 (50mM HEPES-KOH pH7.5, 140mM NaCl, 1mM EDTA, 10% Glycerol, 0.5% NP-40, 0.25% Triton X-100, and protease inhibitors) and rotated at 4°C for 10 min, followed by centrifugation (250× g , 5 min at room temperature). ..

    Centrifugation:

    Article Title: An atlas of lamina-associated chromatin across twelve human cell types reveals an intermediate chromatin subtype
    Article Snippet: .. For ChIP, 30μL protein G magnetic beads (per ChIP sample; Dynal) were washed 3 times in blocking buffer (0.5% BSA in PBS); beads were resuspended in 250μL blocking buffer and 2μg antibody (Lamin B1: ab16048 [Abcam]; H3K9me2: ab1220 [Abcam]) and rotated at 4°C for at least 6 h. Crude nuclei were isolated from frozen crosslinked cells as follows: cell pellet (from 10cm plate) was resuspended in 10mL cold Lysis Buffer 1 (50mM HEPES-KOH pH7.5, 140mM NaCl, 1mM EDTA, 10% Glycerol, 0.5% NP-40, 0.25% Triton X-100, and protease inhibitors) and rotated at 4°C for 10 min, followed by centrifugation (250× g , 5 min at room temperature). ..



    Similar Products

    94
    MedChemExpress hy k1079 deposited data mrna microarray data
    Hy K1079 Deposited Data Mrna Microarray Data, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/deposited+data+microarray+data/One+Step+TUNEL+Apoptosis+Detection+Kit/pm38758648-183-178-176
    Average 94 stars, based on 1 article reviews
    hy k1079 deposited data mrna microarray data - by Bioz Stars, 2026-11
    94/100 stars
      Buy from Supplier

    94
    Cytoskeleton Inc assays signal seeker acetyl lysine detection kit cytoskeleton bk163 deposited data liver microarray analysis gene expression omnibus geo
    Assays Signal Seeker Acetyl Lysine Detection Kit Cytoskeleton Bk163 Deposited Data Liver Microarray Analysis Gene Expression Omnibus Geo, supplied by Cytoskeleton Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/deposited+data+microarray+data/Signal-Seeker+Acetyl-Lysine+Detection+Kit/pm32413334-278-72-77
    Average 94 stars, based on 1 article reviews
    assays signal seeker acetyl lysine detection kit cytoskeleton bk163 deposited data liver microarray analysis gene expression omnibus geo - by Bioz Stars, 2026-11
    94/100 stars
      Buy from Supplier

    96
    R&D Systems dy417 deposited data microarray dataset peritoneal macrophages
    Dy417 Deposited Data Microarray Dataset Peritoneal Macrophages, supplied by R&D Systems, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/deposited+data+microarray+data/Mouse+IL-10+DuoSet+ELISA/pm32049012-206-216-213
    Average 96 stars, based on 1 article reviews
    dy417 deposited data microarray dataset peritoneal macrophages - by Bioz Stars, 2026-11
    96/100 stars
      Buy from Supplier

    99
    Illumina Inc deposited data microarray data
    Deposited Data Microarray Data, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/deposited+data+microarray+data/pm31775048-185-125-123
    Average 99 stars, based on 1 article reviews
    deposited data microarray data - by Bioz Stars, 2026-11
    99/100 stars
      Buy from Supplier

    96
    Genecopoeia lf032 deposited data rna microarray ncbi geo geo
    Lf032 Deposited Data Rna Microarray Ncbi Geo Geo, supplied by Genecopoeia, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/deposited+data+microarray+data/Secrete-Pair+Dual+Luminescence+Assay+Kit/pm31543404-181-32-30
    Average 96 stars, based on 1 article reviews
    lf032 deposited data rna microarray ncbi geo geo - by Bioz Stars, 2026-11
    96/100 stars
      Buy from Supplier

    97
    Danaher Inc rpn2232 deposited data microarray
    Rpn2232 Deposited Data Microarray, supplied by Danaher Inc, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/deposited+data+microarray+data/Amersham+ECL+Prime+Western+Blotting+Detection+Reagent/pm31618630-185-139-136
    Average 97 stars, based on 1 article reviews
    rpn2232 deposited data microarray - by Bioz Stars, 2026-11
    97/100 stars
      Buy from Supplier

    99
    TaKaRa deposited data raw gene microarray data
    Deposited Data Raw Gene Microarray Data, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/deposited+data+microarray+data/TB+Green+Premix+Ex+Taq/pm30067981-234-203-201
    Average 99 stars, based on 1 article reviews
    deposited data raw gene microarray data - by Bioz Stars, 2026-11
    99/100 stars
      Buy from Supplier

    99
    TaKaRa race clonetech 634858 myone streptavine beads thermo 65601 deposited data microarray data geo gse89825 experimental models
    Race Clonetech 634858 Myone Streptavine Beads Thermo 65601 Deposited Data Microarray Data Geo Gse89825 Experimental Models, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/deposited+data+microarray+data/SMARTer+RACE+5%E2%80%99%2F3%E2%80%99+Kit/pm29395921-393-80-81
    Average 99 stars, based on 1 article reviews
    race clonetech 634858 myone streptavine beads thermo 65601 deposited data microarray data geo gse89825 experimental models - by Bioz Stars, 2026-11
    99/100 stars
      Buy from Supplier

    96
    Illumina Inc deposited data gene expression microarray
    Deposited Data Gene Expression Microarray, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/deposited+data+microarray+data/pm30096312-291-271-269
    Average 96 stars, based on 1 article reviews
    deposited data gene expression microarray - by Bioz Stars, 2026-11
    96/100 stars
      Buy from Supplier

    Image Search Results