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densitometry scan quantification  (Bio-Rad)


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    Structured Review

    Bio-Rad densitometry scan quantification
    A, B : Purified human HK (20, 40, 80, and 110ng) and 0.5μL of plasma from five male healthy donors, (H1 to H5) (A), and five females (H6 to H10) (B) were subjected to SDS-PAGE. The signal of HK native chain (N-HK), light chain (LC-HK), and cleaved light chain (cLC-HK) were quantified by <t>densitometry</t> and N-HK concentration was evaluated by the displayed linear regression. C, D : Box-plot displaying 5–95% range ( italic fonts), median ( bold fonts), and interquartile values of plasma concentration of N-HK (C) and HK cleavage (D), measured as in panel A. densito: densitometry in 10 7 arbitrary units; NS: non-significant; ****: p <10 −4 (Mann-Whitney-Wilcoxon test).
    Densitometry Scan Quantification, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 99/100, based on 36302 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/densitometry+quantifications/Image+Lab+Software/pmc05042432-50-12-25
    Average 99 stars, based on 36302 article reviews
    densitometry scan quantification - by Bioz Stars, 2026-09
    99/100 stars

    Images

    1) Product Images from "Kininogen Cleavage Assay: Diagnostic Assistance for Kinin-Mediated Angioedema Conditions"

    Article Title: Kininogen Cleavage Assay: Diagnostic Assistance for Kinin-Mediated Angioedema Conditions

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0163958

    A, B : Purified human HK (20, 40, 80, and 110ng) and 0.5μL of plasma from five male healthy donors, (H1 to H5) (A), and five females (H6 to H10) (B) were subjected to SDS-PAGE. The signal of HK native chain (N-HK), light chain (LC-HK), and cleaved light chain (cLC-HK) were quantified by densitometry and N-HK concentration was evaluated by the displayed linear regression. C, D : Box-plot displaying 5–95% range ( italic fonts), median ( bold fonts), and interquartile values of plasma concentration of N-HK (C) and HK cleavage (D), measured as in panel A. densito: densitometry in 10 7 arbitrary units; NS: non-significant; ****: p <10 −4 (Mann-Whitney-Wilcoxon test).
    Figure Legend Snippet: A, B : Purified human HK (20, 40, 80, and 110ng) and 0.5μL of plasma from five male healthy donors, (H1 to H5) (A), and five females (H6 to H10) (B) were subjected to SDS-PAGE. The signal of HK native chain (N-HK), light chain (LC-HK), and cleaved light chain (cLC-HK) were quantified by densitometry and N-HK concentration was evaluated by the displayed linear regression. C, D : Box-plot displaying 5–95% range ( italic fonts), median ( bold fonts), and interquartile values of plasma concentration of N-HK (C) and HK cleavage (D), measured as in panel A. densito: densitometry in 10 7 arbitrary units; NS: non-significant; ****: p <10 −4 (Mann-Whitney-Wilcoxon test).

    Techniques Used: Purification, Clinical Proteomics, SDS Page, Concentration Assay, MANN-WHITNEY

    A : Purified human HK (20, 40, 80, and 110ng) and 0.5μL of plasma from nC1Inh-AE male patients (M1 to M5) were subjected to SDS-PAGE. The signal of HK native chain (N-HK), light chain (LC-HK), and cleaved light chain (cLC-HK) were quantified by densitometry and N-HK concentration by the displayed linear regression. B, C : Box-plot displaying 5–95% range ( italic fonts), median ( bold fonts), and interquartile values of plasma concentration of N-HK (B) and HK cleavage (C), measured as in panel A, compared to the healthy cohort studied in . D, E, F : as in A, B, C, concerning female patients (W1 to W5). densito: densitometry in 10 7 arbitrary units; ****: p <10 −4 (Mann-Whitney-Wilcoxon test); nC1Inh-AE: angioedema with normal C1 inhibitor.
    Figure Legend Snippet: A : Purified human HK (20, 40, 80, and 110ng) and 0.5μL of plasma from nC1Inh-AE male patients (M1 to M5) were subjected to SDS-PAGE. The signal of HK native chain (N-HK), light chain (LC-HK), and cleaved light chain (cLC-HK) were quantified by densitometry and N-HK concentration by the displayed linear regression. B, C : Box-plot displaying 5–95% range ( italic fonts), median ( bold fonts), and interquartile values of plasma concentration of N-HK (B) and HK cleavage (C), measured as in panel A, compared to the healthy cohort studied in . D, E, F : as in A, B, C, concerning female patients (W1 to W5). densito: densitometry in 10 7 arbitrary units; ****: p <10 −4 (Mann-Whitney-Wilcoxon test); nC1Inh-AE: angioedema with normal C1 inhibitor.

    Techniques Used: Purification, Clinical Proteomics, SDS Page, Concentration Assay, MANN-WHITNEY

    High-molecular-weight kininogen (HK) molecular pattern of plasma samples from healthy women taking or not estrogen/progestin combination (OP; Healthy+OP, n = 32; Healthy-OP, n = 30) and from nC1Inh-AE patients taking OP and ≥2 months after OP withdrawal (nC1INh-AE+/-OP, n = 13). A : Analysis of plasma samples (0.5μL/lane) from six patients (O1–O6) and the corresponding figures. N-HK concentration of P1 and P2 was evaluated by the displayed linear regression, kinin-forming activity was determined as in , pathological values were labelled in bold fonts. B, C : Box-plot displaying 5–95% range ( italic fonts), median ( bold fonts), and interquartile values of the plasma concentration of N-HK (B) and cleaved HK species (C), measured as in panel A. D, E : Details of the paired samples of panel B and C respectively analysed using the Wilcoxon matched-pairs signed rank test. densito: densitometry in 10 7 arbitrary units; NS : non-significant; *: p <0.05; **: p <0.01; ***: p <0.001; ****: p <10 −4 (non-parametric tests); N-HK, LC-HK, cLC-HK: native chain, light chain, cleaved light chain of HK respectively; nC1Inh-AE: angioedema with normal C1 inhibitor.
    Figure Legend Snippet: High-molecular-weight kininogen (HK) molecular pattern of plasma samples from healthy women taking or not estrogen/progestin combination (OP; Healthy+OP, n = 32; Healthy-OP, n = 30) and from nC1Inh-AE patients taking OP and ≥2 months after OP withdrawal (nC1INh-AE+/-OP, n = 13). A : Analysis of plasma samples (0.5μL/lane) from six patients (O1–O6) and the corresponding figures. N-HK concentration of P1 and P2 was evaluated by the displayed linear regression, kinin-forming activity was determined as in , pathological values were labelled in bold fonts. B, C : Box-plot displaying 5–95% range ( italic fonts), median ( bold fonts), and interquartile values of the plasma concentration of N-HK (B) and cleaved HK species (C), measured as in panel A. D, E : Details of the paired samples of panel B and C respectively analysed using the Wilcoxon matched-pairs signed rank test. densito: densitometry in 10 7 arbitrary units; NS : non-significant; *: p <0.05; **: p <0.01; ***: p <0.001; ****: p <10 −4 (non-parametric tests); N-HK, LC-HK, cLC-HK: native chain, light chain, cleaved light chain of HK respectively; nC1Inh-AE: angioedema with normal C1 inhibitor.

    Techniques Used: High Molecular Weight, Clinical Proteomics, Concentration Assay, Activity Assay

    A : High-molecular-weight kininogen (HK) molecular pattern of plasma samples (0.5μL/lane) from two nC1Inh-AE patients (W6, M6) less than 24h after the onset of an AE attack (Att) and at least two weeks after remission (Rem), analysed as in . B : Same as for A, with plasma samples from three patients (K1–K3) with ACEi-AE and low kinin catabolism (L-Cat) and three patients (M7–M9) with nC1Inh-AE and normal kinin catabolism (N-Cat). N-HK concentrations were evaluated by the displayed linear regressions, kinin-forming activity was determined as in , pathological values were labelled in bold font. densito: densitometry in 10 7 arbitrary units; N-HK, LC-HK, cLC-HK: native chain, light chain, cleaved light chain of HK respectively.
    Figure Legend Snippet: A : High-molecular-weight kininogen (HK) molecular pattern of plasma samples (0.5μL/lane) from two nC1Inh-AE patients (W6, M6) less than 24h after the onset of an AE attack (Att) and at least two weeks after remission (Rem), analysed as in . B : Same as for A, with plasma samples from three patients (K1–K3) with ACEi-AE and low kinin catabolism (L-Cat) and three patients (M7–M9) with nC1Inh-AE and normal kinin catabolism (N-Cat). N-HK concentrations were evaluated by the displayed linear regressions, kinin-forming activity was determined as in , pathological values were labelled in bold font. densito: densitometry in 10 7 arbitrary units; N-HK, LC-HK, cLC-HK: native chain, light chain, cleaved light chain of HK respectively.

    Techniques Used: High Molecular Weight, Clinical Proteomics, Activity Assay

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    Article Snippet: After washing and incubating with an appropriate horse radish peroxidase (HRP) secondary antibody (HRP goat anti-mouse 51782504 and HRP goat anti-rabbit 17065156; Bio-Rad Laboratories) and following TBST washes, the membranes were coated with chemiluminescent substrate (West Femto; Thermo Fisher Scientific). .. Images were acquired using Image Lab software (Bio-Rad Laboratories). ..

    Article Title: Modified tumor uptake and biodistribution of nanoparticles coated with small extracellular vesicle membranes derived from distinct tumor cell lines
    Article Snippet: After washing, protein bands were visualized using SuperSignal West Femto (34096, ThermoFisher) and imaged on ChemiDoc MP imaging system (Bio-Rad). .. The signal intensity of bands were quantified utilizing ImageLab software (Bio-Rad). ..

    Article Title: Ribosomal RNA processing impairments in a B cell immunodeficient patient with WDR75 variants
    Article Snippet: Chemiluminescence detection was performed using SuperSignal West Femto Maximum Sensitivity Substrate (Thermo Fisher Scientific) on a ChemiDoc XRS system (Bio-Rad). .. The resulting images were analyzed using Image Lab 4.0 software (Bio-Rad). ..

    Article Title: NASP functions in the cytoplasm to prevent histone H3 aggregation during early embryogenesis
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    Article Title: AAV-mediated gene transfer of a novel microdystrophin ameliorates pathology and enhances muscle function in a mouse model of DMD
    Article Snippet: ECL detection reagents (GE Healthcare, Catalog # RPN 2232) were added, and images of the bands were taken using the ChemiDoc XRS+system (Bio-Rad, USA). .. ImageLab software (Bio-Rad) was used to quantitate the levels of dystrophin/microdystrophin on membranes and myosin heavy chain on the gel following Coomassie Blue staining. ..

    Article Title: Influence of diet-induced obesity and voluntary exercise training on cardiac lipids and mitochondrial function in mice
    Article Snippet: Blots were covered in Clarity Max Chemiluminescent reagent (Bio-Rad) and visualized using the ChemiDoc XRS+ Imaging System (Bio-Rad). .. Densitometry was analyzed using ImageLab software (V6.0.1; Bio-Rad). .. Immunoblotting was performed with antibodies against lysocardiolipin acyltransferase 1(LCLAT1, PA5-25627; Thermo Fisher Scientific), PGC-1α (ab191838; Abcam, Cambridge, UK), MFN2 (PA5-118059; Thermo Fisher Scientific), ANP (sc-18811; Santa Cruz, Dallas, TX, USA), tumor necrosis factor alpha (TNFα, 3707; Cell Signaling, Danvers, MA, USA), phosphorylated adenosine monophosphate-activated protein kinase (AMPK, 2531; Cell Signaling), AMPKα (2532; Cell Signaling), Perilipin 5 (PA1-46215; Thermo Fisher Scientific), translocase of outer mitochondria membrane 70 (TOM70, 65675; Cell Signaling), cytochrome c oxidase subunit 4 (COXIV, 4844; Cell Signaling), and calnexin (208880; Merck, Rahway, NJ, USA).

    Article Title: Avian herpesvirus-specific LORF5 is a late gene, interacts with 19 viral and 111 host proteins, critical for virulence of Duck plague virus
    Article Snippet: .. Average plaque sizes were measured using Image-Pro Plus software (Bio-Rad, CA, USA). ..

    Western Blot:

    Article Title: NASP functions in the cytoplasm to prevent histone H3 aggregation during early embryogenesis
    Article Snippet: Blots were imaged using the Bio-Rad ChemiDoc MP Imaging System. .. The western blots were quantified using Bio-Rad Image Lab software. ..

    Staining:

    Article Title: AAV-mediated gene transfer of a novel microdystrophin ameliorates pathology and enhances muscle function in a mouse model of DMD
    Article Snippet: ECL detection reagents (GE Healthcare, Catalog # RPN 2232) were added, and images of the bands were taken using the ChemiDoc XRS+system (Bio-Rad, USA). .. ImageLab software (Bio-Rad) was used to quantitate the levels of dystrophin/microdystrophin on membranes and myosin heavy chain on the gel following Coomassie Blue staining. ..

    Imaging:

    Article Title: Exercise attenuates stress-related signaling as sensed by higher phosphorylation of small heat shock proteins in skeletal muscle from older individuals
    Article Snippet: .. Chemiluminescent substrate (ECL, Clarity; Bio-Rad Laboratories, Sydney, Australia) enabled imaging of dots using a Chemidoc MP and Image Lab software (Bio-Rad Laboratories). ..



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    Bio-Rad densitometry scan quantification
    A, B : Purified human HK (20, 40, 80, and 110ng) and 0.5μL of plasma from five male healthy donors, (H1 to H5) (A), and five females (H6 to H10) (B) were subjected to SDS-PAGE. The signal of HK native chain (N-HK), light chain (LC-HK), and cleaved light chain (cLC-HK) were quantified by <t>densitometry</t> and N-HK concentration was evaluated by the displayed linear regression. C, D : Box-plot displaying 5–95% range ( italic fonts), median ( bold fonts), and interquartile values of plasma concentration of N-HK (C) and HK cleavage (D), measured as in panel A. densito: densitometry in 10 7 arbitrary units; NS: non-significant; ****: p <10 −4 (Mann-Whitney-Wilcoxon test).
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    Image Search Results


    A, B : Purified human HK (20, 40, 80, and 110ng) and 0.5μL of plasma from five male healthy donors, (H1 to H5) (A), and five females (H6 to H10) (B) were subjected to SDS-PAGE. The signal of HK native chain (N-HK), light chain (LC-HK), and cleaved light chain (cLC-HK) were quantified by densitometry and N-HK concentration was evaluated by the displayed linear regression. C, D : Box-plot displaying 5–95% range ( italic fonts), median ( bold fonts), and interquartile values of plasma concentration of N-HK (C) and HK cleavage (D), measured as in panel A. densito: densitometry in 10 7 arbitrary units; NS: non-significant; ****: p <10 −4 (Mann-Whitney-Wilcoxon test).

    Journal: PLoS ONE

    Article Title: Kininogen Cleavage Assay: Diagnostic Assistance for Kinin-Mediated Angioedema Conditions

    doi: 10.1371/journal.pone.0163958

    Figure Lengend Snippet: A, B : Purified human HK (20, 40, 80, and 110ng) and 0.5μL of plasma from five male healthy donors, (H1 to H5) (A), and five females (H6 to H10) (B) were subjected to SDS-PAGE. The signal of HK native chain (N-HK), light chain (LC-HK), and cleaved light chain (cLC-HK) were quantified by densitometry and N-HK concentration was evaluated by the displayed linear regression. C, D : Box-plot displaying 5–95% range ( italic fonts), median ( bold fonts), and interquartile values of plasma concentration of N-HK (C) and HK cleavage (D), measured as in panel A. densito: densitometry in 10 7 arbitrary units; NS: non-significant; ****: p <10 −4 (Mann-Whitney-Wilcoxon test).

    Article Snippet: Using enhanced chemiluminescence (Clarity Western ECL TM substrate, Biorad, Hercules, USA) and densitometry scan quantification (ChemiDoc TM XRS+ System camera and Image Lab TM software, Bio-Rad, Hercules, USA), three molecular species were retained for data processing: native protein (N-HK, 120kDa), light chain (LC-HK, 56kDa), and cleaved light chain (cLC-HK, 46kDa).

    Techniques: Purification, Clinical Proteomics, SDS Page, Concentration Assay, MANN-WHITNEY

    A : Purified human HK (20, 40, 80, and 110ng) and 0.5μL of plasma from nC1Inh-AE male patients (M1 to M5) were subjected to SDS-PAGE. The signal of HK native chain (N-HK), light chain (LC-HK), and cleaved light chain (cLC-HK) were quantified by densitometry and N-HK concentration by the displayed linear regression. B, C : Box-plot displaying 5–95% range ( italic fonts), median ( bold fonts), and interquartile values of plasma concentration of N-HK (B) and HK cleavage (C), measured as in panel A, compared to the healthy cohort studied in . D, E, F : as in A, B, C, concerning female patients (W1 to W5). densito: densitometry in 10 7 arbitrary units; ****: p <10 −4 (Mann-Whitney-Wilcoxon test); nC1Inh-AE: angioedema with normal C1 inhibitor.

    Journal: PLoS ONE

    Article Title: Kininogen Cleavage Assay: Diagnostic Assistance for Kinin-Mediated Angioedema Conditions

    doi: 10.1371/journal.pone.0163958

    Figure Lengend Snippet: A : Purified human HK (20, 40, 80, and 110ng) and 0.5μL of plasma from nC1Inh-AE male patients (M1 to M5) were subjected to SDS-PAGE. The signal of HK native chain (N-HK), light chain (LC-HK), and cleaved light chain (cLC-HK) were quantified by densitometry and N-HK concentration by the displayed linear regression. B, C : Box-plot displaying 5–95% range ( italic fonts), median ( bold fonts), and interquartile values of plasma concentration of N-HK (B) and HK cleavage (C), measured as in panel A, compared to the healthy cohort studied in . D, E, F : as in A, B, C, concerning female patients (W1 to W5). densito: densitometry in 10 7 arbitrary units; ****: p <10 −4 (Mann-Whitney-Wilcoxon test); nC1Inh-AE: angioedema with normal C1 inhibitor.

    Article Snippet: Using enhanced chemiluminescence (Clarity Western ECL TM substrate, Biorad, Hercules, USA) and densitometry scan quantification (ChemiDoc TM XRS+ System camera and Image Lab TM software, Bio-Rad, Hercules, USA), three molecular species were retained for data processing: native protein (N-HK, 120kDa), light chain (LC-HK, 56kDa), and cleaved light chain (cLC-HK, 46kDa).

    Techniques: Purification, Clinical Proteomics, SDS Page, Concentration Assay, MANN-WHITNEY

    High-molecular-weight kininogen (HK) molecular pattern of plasma samples from healthy women taking or not estrogen/progestin combination (OP; Healthy+OP, n = 32; Healthy-OP, n = 30) and from nC1Inh-AE patients taking OP and ≥2 months after OP withdrawal (nC1INh-AE+/-OP, n = 13). A : Analysis of plasma samples (0.5μL/lane) from six patients (O1–O6) and the corresponding figures. N-HK concentration of P1 and P2 was evaluated by the displayed linear regression, kinin-forming activity was determined as in , pathological values were labelled in bold fonts. B, C : Box-plot displaying 5–95% range ( italic fonts), median ( bold fonts), and interquartile values of the plasma concentration of N-HK (B) and cleaved HK species (C), measured as in panel A. D, E : Details of the paired samples of panel B and C respectively analysed using the Wilcoxon matched-pairs signed rank test. densito: densitometry in 10 7 arbitrary units; NS : non-significant; *: p <0.05; **: p <0.01; ***: p <0.001; ****: p <10 −4 (non-parametric tests); N-HK, LC-HK, cLC-HK: native chain, light chain, cleaved light chain of HK respectively; nC1Inh-AE: angioedema with normal C1 inhibitor.

    Journal: PLoS ONE

    Article Title: Kininogen Cleavage Assay: Diagnostic Assistance for Kinin-Mediated Angioedema Conditions

    doi: 10.1371/journal.pone.0163958

    Figure Lengend Snippet: High-molecular-weight kininogen (HK) molecular pattern of plasma samples from healthy women taking or not estrogen/progestin combination (OP; Healthy+OP, n = 32; Healthy-OP, n = 30) and from nC1Inh-AE patients taking OP and ≥2 months after OP withdrawal (nC1INh-AE+/-OP, n = 13). A : Analysis of plasma samples (0.5μL/lane) from six patients (O1–O6) and the corresponding figures. N-HK concentration of P1 and P2 was evaluated by the displayed linear regression, kinin-forming activity was determined as in , pathological values were labelled in bold fonts. B, C : Box-plot displaying 5–95% range ( italic fonts), median ( bold fonts), and interquartile values of the plasma concentration of N-HK (B) and cleaved HK species (C), measured as in panel A. D, E : Details of the paired samples of panel B and C respectively analysed using the Wilcoxon matched-pairs signed rank test. densito: densitometry in 10 7 arbitrary units; NS : non-significant; *: p <0.05; **: p <0.01; ***: p <0.001; ****: p <10 −4 (non-parametric tests); N-HK, LC-HK, cLC-HK: native chain, light chain, cleaved light chain of HK respectively; nC1Inh-AE: angioedema with normal C1 inhibitor.

    Article Snippet: Using enhanced chemiluminescence (Clarity Western ECL TM substrate, Biorad, Hercules, USA) and densitometry scan quantification (ChemiDoc TM XRS+ System camera and Image Lab TM software, Bio-Rad, Hercules, USA), three molecular species were retained for data processing: native protein (N-HK, 120kDa), light chain (LC-HK, 56kDa), and cleaved light chain (cLC-HK, 46kDa).

    Techniques: High Molecular Weight, Clinical Proteomics, Concentration Assay, Activity Assay

    A : High-molecular-weight kininogen (HK) molecular pattern of plasma samples (0.5μL/lane) from two nC1Inh-AE patients (W6, M6) less than 24h after the onset of an AE attack (Att) and at least two weeks after remission (Rem), analysed as in . B : Same as for A, with plasma samples from three patients (K1–K3) with ACEi-AE and low kinin catabolism (L-Cat) and three patients (M7–M9) with nC1Inh-AE and normal kinin catabolism (N-Cat). N-HK concentrations were evaluated by the displayed linear regressions, kinin-forming activity was determined as in , pathological values were labelled in bold font. densito: densitometry in 10 7 arbitrary units; N-HK, LC-HK, cLC-HK: native chain, light chain, cleaved light chain of HK respectively.

    Journal: PLoS ONE

    Article Title: Kininogen Cleavage Assay: Diagnostic Assistance for Kinin-Mediated Angioedema Conditions

    doi: 10.1371/journal.pone.0163958

    Figure Lengend Snippet: A : High-molecular-weight kininogen (HK) molecular pattern of plasma samples (0.5μL/lane) from two nC1Inh-AE patients (W6, M6) less than 24h after the onset of an AE attack (Att) and at least two weeks after remission (Rem), analysed as in . B : Same as for A, with plasma samples from three patients (K1–K3) with ACEi-AE and low kinin catabolism (L-Cat) and three patients (M7–M9) with nC1Inh-AE and normal kinin catabolism (N-Cat). N-HK concentrations were evaluated by the displayed linear regressions, kinin-forming activity was determined as in , pathological values were labelled in bold font. densito: densitometry in 10 7 arbitrary units; N-HK, LC-HK, cLC-HK: native chain, light chain, cleaved light chain of HK respectively.

    Article Snippet: Using enhanced chemiluminescence (Clarity Western ECL TM substrate, Biorad, Hercules, USA) and densitometry scan quantification (ChemiDoc TM XRS+ System camera and Image Lab TM software, Bio-Rad, Hercules, USA), three molecular species were retained for data processing: native protein (N-HK, 120kDa), light chain (LC-HK, 56kDa), and cleaved light chain (cLC-HK, 46kDa).

    Techniques: High Molecular Weight, Clinical Proteomics, Activity Assay