ampliconez targeted amplicon deep sequencing (Azenta)
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Ampliconez Targeted Amplicon Deep Sequencing, supplied by Azenta, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/deep+sequencing+%26+microarray+core+facility/amplicon+sequencing/pmc12948676-228-4-9
Average 86 stars, based on 1 article reviews
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Gel Extraction:Article Title: Inverted Alu repeats in loop-out exon skipping across hominoid evolution Article Snippet: .. Exon skipping bands with the expected size were excised, extracted (PureLink Quick Gel Extraction, Invitrogen), and confirmed by either Sanger sequencing or Article Title: Sequence-directed covalent protein-RNA linkages in a single step using engineered HUH-tags. Article Snippet: .. The amplicons were then purified via gel extraction and sent for Sequencing:Article Title: Inverted Alu repeats in loop-out exon skipping across hominoid evolution Article Snippet: .. Exon skipping bands with the expected size were excised, extracted (PureLink Quick Gel Extraction, Invitrogen), and confirmed by either Sanger sequencing or Article Title: Sequence-directed covalent protein-RNA linkages in a single step using engineered HUH-tags. Article Snippet: .. The amplicons were then purified via gel extraction and sent for Article Title: Direct pharmacological targeting of asparagine synthetase to overcome resistance to L-asparaginase in ALL therapy. Article Snippet: .. 620 The PCR products were gel-purified and sent for Article Title: Rapid screening of genome edited strawberry (Fragaria ×ananassa) regenerants using high-resolution melting analysis followed by Amplicon sequencing. Article Snippet: Amplicon length was confirmed using gel electrophoresis and remaining PCR products were purified using the Zymo DNA Clean & ACCEPTED MANUSCRIPT AR TIC LE IN PR ES S ARTICLE IN PRESS Concentrator-25 kit (Zymo Research; D4034). .. Samples were then submitted to Genewiz for Amplification:Article Title: Inverted Alu repeats in loop-out exon skipping across hominoid evolution Article Snippet: .. Exon skipping bands with the expected size were excised, extracted (PureLink Quick Gel Extraction, Invitrogen), and confirmed by either Sanger sequencing or Article Title: Sequence-directed covalent protein-RNA linkages in a single step using engineered HUH-tags. Article Snippet: .. The amplicons were then purified via gel extraction and sent for Article Title: Direct pharmacological targeting of asparagine synthetase to overcome resistance to L-asparaginase in ALL therapy. Article Snippet: .. 620 The PCR products were gel-purified and sent for Article Title: Rapid screening of genome edited strawberry (Fragaria ×ananassa) regenerants using high-resolution melting analysis followed by Amplicon sequencing. Article Snippet: Amplicon length was confirmed using gel electrophoresis and remaining PCR products were purified using the Zymo DNA Clean & ACCEPTED MANUSCRIPT AR TIC LE IN PR ES S ARTICLE IN PRESS Concentrator-25 kit (Zymo Research; D4034). .. Samples were then submitted to Genewiz for Article Title: Proteolysis activity mapping and substrate discovery platform for identifying tumor-activated biosensors. Article Snippet: The plasmid backbone was digested with XhoI and BamHI restriction enzymes (Thermo Fisher Scientific, FD0694 and FD0054) and gel purified. .. Samples for Purification:Article Title: Sequence-directed covalent protein-RNA linkages in a single step using engineered HUH-tags. Article Snippet: .. The amplicons were then purified via gel extraction and sent for Next-Generation Sequencing:Article Title: Sequence-directed covalent protein-RNA linkages in a single step using engineered HUH-tags. Article Snippet: .. The amplicons were then purified via gel extraction and sent for Article Title: Proteolysis activity mapping and substrate discovery platform for identifying tumor-activated biosensors. Article Snippet: The plasmid backbone was digested with XhoI and BamHI restriction enzymes (Thermo Fisher Scientific, FD0694 and FD0054) and gel purified. .. Samples for other:Article Title: Rational design of mechanically active RNAs: de novo engineering of functional exoribonuclease-resistant RNAs. Article Snippet: PCR products were purified on a 10% agarose gel, eluted with GeneJET gel extraction kit (Thermo Scientific) and analyzed by Article Title: Exploiting conformational changes in a caffeine aptamer to engineer synthetic RNA devices. Article Snippet: One hundred twenty-five picomoles of RNA in 150 μl ultrapure water were heated to 95◦C for 5 min and subsequently cooled on ice for 2 min before adding 75 μl folding buffer (333 mM NaCl, 333 mM HEPES pH 8, 33 mM MgCl2 ) and incubating at 37◦C for 20 min. Ninety microliters of refolded RNA was added to 10 μl of 100 mM 1-methyl- 7-nitroisatoicanhydride (1M7, Sigma–Aldrich) in water-free dimethyl sulfoxide (Sigma–Aldrich) and incubated at 37◦C for 2 min. Article Title: Rational design of mechanically active RNAs: de novo engineering of functional exoribonuclease-resistant RNAs. Article Snippet: The transcripts were PCR-amplified to introduce adapter sequences for Polymerase Chain Reaction:Article Title: Direct pharmacological targeting of asparagine synthetase to overcome resistance to L-asparaginase in ALL therapy. Article Snippet: .. 620 The PCR products were gel-purified and sent for Article Title: Proteolysis activity mapping and substrate discovery platform for identifying tumor-activated biosensors. Article Snippet: The plasmid backbone was digested with XhoI and BamHI restriction enzymes (Thermo Fisher Scientific, FD0694 and FD0054) and gel purified. .. Samples for |

