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ti e deconvolution inverted microscope  (Nikon)


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    Nikon ti e deconvolution inverted microscope
    Ti E Deconvolution Inverted Microscope, supplied by Nikon, used in various techniques. Bioz Stars score: 99/100, based on 14740 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/deconvolution/Inverted+Microscopes/pm31087362-87-20-19
    Average 99 stars, based on 14740 article reviews
    ti e deconvolution inverted microscope - by Bioz Stars, 2026-10
    99/100 stars

    Images

    Related Articles

    Construct:

    Article Title: A smart magneto-responsive ternary 3D platform for actively steering stereoscopic infiltration and chronological osteogenic differentiation of BMSCs.
    Article Snippet: The limited effective infiltration of cells into the interior of scaffolds remains a critical bottleneck limiting the therapeutic application of bone tissue engineering.. To address this challenge, this study developed a physically self-assembled ternary smart magneto-responsive composite platform (MGO/CS) based on chitosan, graphene oxide, and superparamagnetic iron oxide nanoparticles (SPIONs).. Although the incorporation of SPIONs reduced the mean pore size of the scaffold from 306.64 ± 52.25 μm to 185.39 ± 28.32 μm (p < 0.05), this smart platform, driven by a 3 mT weak static magnetic field (SMF), successfully mitigated the dependence of the threedimensional colonization of stem cells on pore size, thereby partially overcoming the constraints imposed by conventional geometric topography on deep cell infiltration.

    Staining:

    Article Title: A smart magneto-responsive ternary 3D platform for actively steering stereoscopic infiltration and chronological osteogenic differentiation of BMSCs.
    Article Snippet: The limited effective infiltration of cells into the interior of scaffolds remains a critical bottleneck limiting the therapeutic application of bone tissue engineering.. To address this challenge, this study developed a physically self-assembled ternary smart magneto-responsive composite platform (MGO/CS) based on chitosan, graphene oxide, and superparamagnetic iron oxide nanoparticles (SPIONs).. Although the incorporation of SPIONs reduced the mean pore size of the scaffold from 306.64 ± 52.25 μm to 185.39 ± 28.32 μm (p < 0.05), this smart platform, driven by a 3 mT weak static magnetic field (SMF), successfully mitigated the dependence of the threedimensional colonization of stem cells on pore size, thereby partially overcoming the constraints imposed by conventional geometric topography on deep cell infiltration.

    Article Title: Amentoflavone mitigates UVB-induced epidermal photoaging by antagonizing PAR2-driven PI3K/Akt/FoxO6 oxidative-stress signaling.
    Article Snippet: Escalating surface ultraviolet-B (UVB) radiation accelerates skin photoaging and underscores the need for safe, plant-derived photoprotectants.. Amentoflavone (AF), the principal biflavonoid of the resurrection plant Selaginella tamariscina, has recognized anti-photoaging activity, yet its epidermal mechanism remains undefined.. This study utilized animal and cellular models to investigate the therapeutic potential of AF (0.1–0.2 mg g 1) against photoaging.

    Microscopy:

    Article Title: A smart magneto-responsive ternary 3D platform for actively steering stereoscopic infiltration and chronological osteogenic differentiation of BMSCs.
    Article Snippet: The limited effective infiltration of cells into the interior of scaffolds remains a critical bottleneck limiting the therapeutic application of bone tissue engineering.. To address this challenge, this study developed a physically self-assembled ternary smart magneto-responsive composite platform (MGO/CS) based on chitosan, graphene oxide, and superparamagnetic iron oxide nanoparticles (SPIONs).. Although the incorporation of SPIONs reduced the mean pore size of the scaffold from 306.64 ± 52.25 μm to 185.39 ± 28.32 μm (p < 0.05), this smart platform, driven by a 3 mT weak static magnetic field (SMF), successfully mitigated the dependence of the threedimensional colonization of stem cells on pore size, thereby partially overcoming the constraints imposed by conventional geometric topography on deep cell infiltration.

    Article Title: Amentoflavone mitigates UVB-induced epidermal photoaging by antagonizing PAR2-driven PI3K/Akt/FoxO6 oxidative-stress signaling.
    Article Snippet: Escalating surface ultraviolet-B (UVB) radiation accelerates skin photoaging and underscores the need for safe, plant-derived photoprotectants.. Amentoflavone (AF), the principal biflavonoid of the resurrection plant Selaginella tamariscina, has recognized anti-photoaging activity, yet its epidermal mechanism remains undefined.. This study utilized animal and cellular models to investigate the therapeutic potential of AF (0.1–0.2 mg g 1) against photoaging.

    Article Title: An All-in-One Nanozyme for Synergistic Diabetic Wound Therapy: NIR-Augmented NO Release and Microenvironment Modulation.
    Article Snippet: Diabetic wound infection remains a devastating threat to human health, largely due to bacterial colonization and increased antibiotic resistance during conventional treatments, and alternative therapeutic strategies are thus urgent to improve diabetic wound healing.. Herein, we developed a multifaceted nanoplatform (CuO@SiO2@NO@Au, CSNA NPs) consisting of a cupric oxide (CuO) core, a mesoporous silicon nanoshell loaded with nitric oxide (NO), and in situ grown ultrasmall Au nanoparticles (NPs) for improved diabetic wound treatment.. The results showed that the preparedCSNANPs exhibited remarkable dual-enzyme mimic activity of glucose oxidase (GOx) and peroxidase (POD), effectively oxidizing glucose to generate gluconic acid, thereby reducing the glucose levels and reversing the acidic woundmicroenvironment.

    Article Title: Integrated fabrication of a shape-adaptable, antioxidative composite stent for effective closure and biological repair of enteroatmospheric fistula
    Article Snippet: .. An inverted fluorescence microscope (Nikon, TiS, Japan) was applied to capture images. ..

    Article Title: Synergistic mitochondrial homeostasis regulation and cholinergic circuits reconstruction via a one-step synthesized multifunctional hydrogel facilitates spinal cord injury repair
    Article Snippet: All images were quantitatively analyzed using NIS-Elements AR software (Version 5.11, Nikon) to ensure the accuracy and reproducibility of experimental results. .. For ROS detection, 10 μM DCFH-DA or DHE working solution was added, followed by incubation at 37 °C in the dark for 30 min. For mtROS detection, PC12 cells was incubated with 5 μM MitoSox Red working solution under the same conditions in the dark for 15 min. Fluorescence images were acquired using a Nikon inverted fluorescence microscope (Nikon, Japan). .. All images were quantitatively analyzed using NIS-Elements AR software (Version 5.11, Nikon) to ensure the accuracy and reproducibility of experimental results.

    other:

    Article Title: Spatiotemporal Synergistic Effects of Injectable Biomaterials With Two Typical 3D Geometries on Macrophage Modulation for Enhanced Tissue Regeneration.
    Article Snippet: Injectable biomaterials with immunomodulatory functions have become a promising strategy to facilitate tissue regeneration.. Material geometry is known to influence macrophage attachment and phenotype, providing opportunities for the rational design of “immune-instructive” topographies to modulate macrophage function.. Here, we developed a biomaterial systems consisting of two different “immune-instructives” 3D geometries: sphere-shaped microspheres (MSs) and rod-shaped short microfibers (MFs) to modulate macrophage function for tissue regeneration.

    Article Title: Metal-Phenolic Nanoplatform Tailored to the Metabolic Dependence of Prostate Cancer to Amplify Cuproptosis and Apoptosis
    Article Snippet: After 24 h of adhesion, cells were treated with FITC-labeled Cu-Phl MPNs (10 μg mL− 1) for 0, 1, 3, and 6 h. Following incubation, nuclei were counterstained with Hoechst 33342, and Cu-Phl MPNs internalization was visualized by inverted fluorescence microscopy (Nikon ECLIPSE Ts2).

    Incubation:

    Article Title: MS4322 is a selective protein arginine methyltransferase 5 degrader with antitumor effects in cervical cancer cells
    Article Snippet: The cell monolayer was scraped with a 10 μl pipette tip to produce a wound, washed with PBS to remove detached cells and the cells were incubated in serum-free DMEM (Gibco; Thermo Fisher Scientific, Inc.). .. Cells were incubated at 37°C for 24 h. Photographs were taken at 0 and 24 h using a Nikon inverted light microscope (Nikon Instruments). .. Wound width analysis was performed with Image-Pro Plus 6.0 software (Media Cybernetics, Inc.).

    Article Title: An All-in-One Nanozyme for Synergistic Diabetic Wound Therapy: NIR-Augmented NO Release and Microenvironment Modulation.
    Article Snippet: Diabetic wound infection remains a devastating threat to human health, largely due to bacterial colonization and increased antibiotic resistance during conventional treatments, and alternative therapeutic strategies are thus urgent to improve diabetic wound healing.. Herein, we developed a multifaceted nanoplatform (CuO@SiO2@NO@Au, CSNA NPs) consisting of a cupric oxide (CuO) core, a mesoporous silicon nanoshell loaded with nitric oxide (NO), and in situ grown ultrasmall Au nanoparticles (NPs) for improved diabetic wound treatment.. The results showed that the preparedCSNANPs exhibited remarkable dual-enzyme mimic activity of glucose oxidase (GOx) and peroxidase (POD), effectively oxidizing glucose to generate gluconic acid, thereby reducing the glucose levels and reversing the acidic woundmicroenvironment.

    Article Title: Synergistic mitochondrial homeostasis regulation and cholinergic circuits reconstruction via a one-step synthesized multifunctional hydrogel facilitates spinal cord injury repair
    Article Snippet: All images were quantitatively analyzed using NIS-Elements AR software (Version 5.11, Nikon) to ensure the accuracy and reproducibility of experimental results. .. For ROS detection, 10 μM DCFH-DA or DHE working solution was added, followed by incubation at 37 °C in the dark for 30 min. For mtROS detection, PC12 cells was incubated with 5 μM MitoSox Red working solution under the same conditions in the dark for 15 min. Fluorescence images were acquired using a Nikon inverted fluorescence microscope (Nikon, Japan). .. All images were quantitatively analyzed using NIS-Elements AR software (Version 5.11, Nikon) to ensure the accuracy and reproducibility of experimental results.

    Light Microscopy:

    Article Title: MS4322 is a selective protein arginine methyltransferase 5 degrader with antitumor effects in cervical cancer cells
    Article Snippet: The cell monolayer was scraped with a 10 μl pipette tip to produce a wound, washed with PBS to remove detached cells and the cells were incubated in serum-free DMEM (Gibco; Thermo Fisher Scientific, Inc.). .. Cells were incubated at 37°C for 24 h. Photographs were taken at 0 and 24 h using a Nikon inverted light microscope (Nikon Instruments). .. Wound width analysis was performed with Image-Pro Plus 6.0 software (Media Cybernetics, Inc.).

    Fluorescence:

    Article Title: Amentoflavone mitigates UVB-induced epidermal photoaging by antagonizing PAR2-driven PI3K/Akt/FoxO6 oxidative-stress signaling.
    Article Snippet: Escalating surface ultraviolet-B (UVB) radiation accelerates skin photoaging and underscores the need for safe, plant-derived photoprotectants.. Amentoflavone (AF), the principal biflavonoid of the resurrection plant Selaginella tamariscina, has recognized anti-photoaging activity, yet its epidermal mechanism remains undefined.. This study utilized animal and cellular models to investigate the therapeutic potential of AF (0.1–0.2 mg g 1) against photoaging.

    Article Title: An All-in-One Nanozyme for Synergistic Diabetic Wound Therapy: NIR-Augmented NO Release and Microenvironment Modulation.
    Article Snippet: Diabetic wound infection remains a devastating threat to human health, largely due to bacterial colonization and increased antibiotic resistance during conventional treatments, and alternative therapeutic strategies are thus urgent to improve diabetic wound healing.. Herein, we developed a multifaceted nanoplatform (CuO@SiO2@NO@Au, CSNA NPs) consisting of a cupric oxide (CuO) core, a mesoporous silicon nanoshell loaded with nitric oxide (NO), and in situ grown ultrasmall Au nanoparticles (NPs) for improved diabetic wound treatment.. The results showed that the preparedCSNANPs exhibited remarkable dual-enzyme mimic activity of glucose oxidase (GOx) and peroxidase (POD), effectively oxidizing glucose to generate gluconic acid, thereby reducing the glucose levels and reversing the acidic woundmicroenvironment.

    Article Title: Integrated fabrication of a shape-adaptable, antioxidative composite stent for effective closure and biological repair of enteroatmospheric fistula
    Article Snippet: .. An inverted fluorescence microscope (Nikon, TiS, Japan) was applied to capture images. ..

    Article Title: Synergistic mitochondrial homeostasis regulation and cholinergic circuits reconstruction via a one-step synthesized multifunctional hydrogel facilitates spinal cord injury repair
    Article Snippet: All images were quantitatively analyzed using NIS-Elements AR software (Version 5.11, Nikon) to ensure the accuracy and reproducibility of experimental results. .. For ROS detection, 10 μM DCFH-DA or DHE working solution was added, followed by incubation at 37 °C in the dark for 30 min. For mtROS detection, PC12 cells was incubated with 5 μM MitoSox Red working solution under the same conditions in the dark for 15 min. Fluorescence images were acquired using a Nikon inverted fluorescence microscope (Nikon, Japan). .. All images were quantitatively analyzed using NIS-Elements AR software (Version 5.11, Nikon) to ensure the accuracy and reproducibility of experimental results.



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    Immune infiltration analysis. (A) Relative proportions of immune cell subpopulations in the control and sepsis groups. (B) Correlation matrix illustrating the associations between different immune cell types. Red indicates a positive correlation, and blue indicates a negative correlation; the intensity of the color and the size of the circles correspond to the strength of the correlation coefficient. (C) Comparison of immune cell abundance between the control and sepsis groups. Blue represents the control group, while yellow represents the sepsis group. (Note: These results are based on <t>CIBERSORT</t> <t>deconvolution</t> of bulk transcriptomic data). (D–G) Correlation analysis between the identified candidate genes and the infiltration levels of various immune cell types. *: p<0.05, **: P<0.01, ***: P<0.001, ns: not significant.
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    Immune infiltration analysis. (A) Relative proportions of immune cell subpopulations in the control and sepsis groups. (B) Correlation matrix illustrating the associations between different immune cell types. Red indicates a positive correlation, and blue indicates a negative correlation; the intensity of the color and the size of the circles correspond to the strength of the correlation coefficient. (C) Comparison of immune cell abundance between the control and sepsis groups. Blue represents the control group, while yellow represents the sepsis group. (Note: These results are based on <t>CIBERSORT</t> <t>deconvolution</t> of bulk transcriptomic data). (D–G) Correlation analysis between the identified candidate genes and the infiltration levels of various immune cell types. *: p<0.05, **: P<0.01, ***: P<0.001, ns: not significant.
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    Image Search Results


    Immune infiltration analysis. (A) Relative proportions of immune cell subpopulations in the control and sepsis groups. (B) Correlation matrix illustrating the associations between different immune cell types. Red indicates a positive correlation, and blue indicates a negative correlation; the intensity of the color and the size of the circles correspond to the strength of the correlation coefficient. (C) Comparison of immune cell abundance between the control and sepsis groups. Blue represents the control group, while yellow represents the sepsis group. (Note: These results are based on CIBERSORT deconvolution of bulk transcriptomic data). (D–G) Correlation analysis between the identified candidate genes and the infiltration levels of various immune cell types. *: p<0.05, **: P<0.01, ***: P<0.001, ns: not significant.

    Journal: Frontiers in Cellular and Infection Microbiology

    Article Title: A synergistic multi-omics approach: causal sepsis drivers identified in activated CD4 + T cells by single-cell RNA sequencing and Mendelian randomization

    doi: 10.3389/fcimb.2026.1749207

    Figure Lengend Snippet: Immune infiltration analysis. (A) Relative proportions of immune cell subpopulations in the control and sepsis groups. (B) Correlation matrix illustrating the associations between different immune cell types. Red indicates a positive correlation, and blue indicates a negative correlation; the intensity of the color and the size of the circles correspond to the strength of the correlation coefficient. (C) Comparison of immune cell abundance between the control and sepsis groups. Blue represents the control group, while yellow represents the sepsis group. (Note: These results are based on CIBERSORT deconvolution of bulk transcriptomic data). (D–G) Correlation analysis between the identified candidate genes and the infiltration levels of various immune cell types. *: p<0.05, **: P<0.01, ***: P<0.001, ns: not significant.

    Article Snippet: While we partially addressed this via CIBERSORT deconvolution to explore neutrophil-related correlations, their specific contribution to the ‘Metabolism–Proteostasis–Immunity’ axis may not be fully captured at the single-cell level.

    Techniques: Control, Comparison

    a Example of deconvolution prediction with FM2FM. Top, input raw fluorescent membrane images (Raw FM 4-64); middle row, true deconvolved fluorescent membrane images (Deconvolved FM 4-64); bottom, FM2FM-predicted image. White squares mark regions zoomed in at right (Block 1 and Block 2). Diagonal dotted lines indicate profile traces in ( b ). Scale bars: full images, 10 µm; zoomed-in blocks, 5 µm. b Fluorescence profiles across the dotted lines in Block 1 (top) and Block 2 (bottom). Light blue, raw FM 4-64; orange, deconvolved FM 4-64; burgundy, FM2FM prediction. c , Structural similarity index measure (SSIM) between FM2FM predicted images and deconvolved images. The distribution of SSIM values across 74 image crops is shown. d Violin plots of cell width, length, surface area, volume, cross-sectional area, convex hull area, eccentricity and solidity calculated from deconvolved FM 4-64 images (orange) and FM2FM-predicted images (burgundy). The white dots and vertical lines within the violin plots represent the medians and standard deviations. P values from statistical comparisons between distributions (ANOVA or Kruskal test; see methods for details) are indicated in each panel. Twenty images (over 1000 cells) were processed and segmented with FMSeg. See the Methods for size calculation details.

    Journal: Communications Biology

    Article Title: Deep-learning deconvolution and segmentation of fluorescent membranes for high-precision bacterial cell-size profiling

    doi: 10.1038/s42003-026-10303-y

    Figure Lengend Snippet: a Example of deconvolution prediction with FM2FM. Top, input raw fluorescent membrane images (Raw FM 4-64); middle row, true deconvolved fluorescent membrane images (Deconvolved FM 4-64); bottom, FM2FM-predicted image. White squares mark regions zoomed in at right (Block 1 and Block 2). Diagonal dotted lines indicate profile traces in ( b ). Scale bars: full images, 10 µm; zoomed-in blocks, 5 µm. b Fluorescence profiles across the dotted lines in Block 1 (top) and Block 2 (bottom). Light blue, raw FM 4-64; orange, deconvolved FM 4-64; burgundy, FM2FM prediction. c , Structural similarity index measure (SSIM) between FM2FM predicted images and deconvolved images. The distribution of SSIM values across 74 image crops is shown. d Violin plots of cell width, length, surface area, volume, cross-sectional area, convex hull area, eccentricity and solidity calculated from deconvolved FM 4-64 images (orange) and FM2FM-predicted images (burgundy). The white dots and vertical lines within the violin plots represent the medians and standard deviations. P values from statistical comparisons between distributions (ANOVA or Kruskal test; see methods for details) are indicated in each panel. Twenty images (over 1000 cells) were processed and segmented with FMSeg. See the Methods for size calculation details.

    Article Snippet: To compare model performance against the membrane deconvolution target (SoftWorx), we conducted qualitative assessments of output images' intensity profiles.

    Techniques: Membrane, Blocking Assay, Fluorescence