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deconvolution module  (Indica Labs)

 
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    Structured Review

    Indica Labs deconvolution module
    Deconvolution Module, supplied by Indica Labs, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/deconvolution+module/algorithm+deconvolution+halo/pm41912809-342-12-19
    Average 86 stars, based on 1 article reviews
    deconvolution module - by Bioz Stars, 2026-10
    86/100 stars

    Images

    Related Articles

    Activation Assay:

    Article Title: Accelerated amyloid neurodegeneration in HIV-1-infected APP-KI Alzheimer’s disease mice
    Article Snippet: For IF image analysis, the Indica-Labs Area Quantification FL v2.3.4 module was used. .. The Indica Labs-Microglial Activation FL v1.0.6 module was used to quantify activated microglia. .. The Indica Labs-Microglial Activation FL v1.0.6 module was used to quantify activated microglia.

    other:

    Article Title: Myeloid reprogramming by poly(I:C) recruits progenitor-exhausted CD8+ T cells and sensitizes rhabdoid tumors to PD-1 blockade
    Article Snippet: Chromogenic revelation was achieved with 3-amino-9-ethylcarbazole (AEC; Abcam, ab64252).

    Article Title: Human lung explants as a predictive platform for evaluating the on-target, off-tumor toxicity of T cell bispecifics
    Article Snippet: Within these areas, the area occupied by DAPI signal was calculated using a standard HALO Algorithm (Indica Labs - Area Quantification FL.v2.3.4), based on manually adjusted DAPI signal thresholds.

    Software:

    Article Title: Non-coding variants in intron 2 of HK1 associated with hyperinsulinism with variable clinical phenotype.
    Article Snippet: Slides were incubated with 21 AC CE PT ED M AN US CR IP T D ow nloaded from https://academ ic.oup.com /jcem /advance-article/doi/10.1210/clinem /dgag108/8513866 by SBD -FFLC H -U SP user on 15 M arch 2026 primary antibodies [rabbit anti-HK1 1:250 (RRID:AB_2117001), and goat anti-insulin 1:500 1 (RRID: AB_2296108), Santa Cruz Biotechnology, Dallas, Texas] overnight at 4 degrees Celsius 2 followed by incubation with the secondary antibodies [488 donkey anti-goat 1:500 3 (RRID:AB_2762833), Cy3 donkey anti-goat 1:500 (RRID:AB_2762840) and DAPI 1:1000] for 4 1 hour in a hydration chamber at room temperature. .. Slides were scanned into ImageScope 5 Software (Leica Biosystems, Deer Park, Illinois) and analyzed using the Indica Cyto-Nuclear FL 6 v1.3 algorithm (Indica Labs, Albuquerque, New Mexico). ..

    Article Title: Quemliclustat and chemotherapy with or without zimberelimab in metastatic pancreatic adenocarcinoma: a randomized phase 1 trial.
    Article Snippet: .. Chromogenic RNAscope images were deconvoluted and converted into pseudofluorescent images using the Deconvolution module (v.2.0.1) in HALO software (v.4.0, Indica Labs). ..

    RNAscope:

    Article Title: Quemliclustat and chemotherapy with or without zimberelimab in metastatic pancreatic adenocarcinoma: a randomized phase 1 trial.
    Article Snippet: .. Chromogenic RNAscope images were deconvoluted and converted into pseudofluorescent images using the Deconvolution module (v.2.0.1) in HALO software (v.4.0, Indica Labs). ..



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    ( A , B ) HUVEC were treated with histamine (100 µM, Sigma-Aldrich) or thrombin (1 U/ml, CalBiochem) for 10 min, or left untreated with equivalent volumes of vehicle (media) as controls. Cells were subsequently fixed, permeabilized and stained for plakoglobin, β-catenin, and VE-cadherin, all visualized in grayscale to ensure unbiased comparative analysis. Higher magnification images (63X) were acquired using a Zeiss LSM 980 confocal microscope equipped with an <t>Airyscan</t> detector ( A ), while lower magnification images (40X) were captured on a Zeiss LSM 880 confocal microscope ( B ). Scale bars, 25 µm.
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    Image Search Results


    ( A , B ) HUVEC were treated with histamine (100 µM, Sigma-Aldrich) or thrombin (1 U/ml, CalBiochem) for 10 min, or left untreated with equivalent volumes of vehicle (media) as controls. Cells were subsequently fixed, permeabilized and stained for plakoglobin, β-catenin, and VE-cadherin, all visualized in grayscale to ensure unbiased comparative analysis. Higher magnification images (63X) were acquired using a Zeiss LSM 980 confocal microscope equipped with an Airyscan detector ( A ), while lower magnification images (40X) were captured on a Zeiss LSM 880 confocal microscope ( B ). Scale bars, 25 µm.

    Journal: The EMBO Journal

    Article Title: Plakoglobin transmits tension across VE-cadherin for vascular leak formation and leukocyte diapedesis

    doi: 10.1038/s44318-026-00732-0

    Figure Lengend Snippet: ( A , B ) HUVEC were treated with histamine (100 µM, Sigma-Aldrich) or thrombin (1 U/ml, CalBiochem) for 10 min, or left untreated with equivalent volumes of vehicle (media) as controls. Cells were subsequently fixed, permeabilized and stained for plakoglobin, β-catenin, and VE-cadherin, all visualized in grayscale to ensure unbiased comparative analysis. Higher magnification images (63X) were acquired using a Zeiss LSM 980 confocal microscope equipped with an Airyscan detector ( A ), while lower magnification images (40X) were captured on a Zeiss LSM 880 confocal microscope ( B ). Scale bars, 25 µm.

    Article Snippet: Zeiss LSM 980 Airyscan acquisitions were processed with Airyscan Joint Deconvolution processing module in ZEN blue software (Zeiss) to improve signal-to-noise and spatial resolution; LSM 880 images were used without further deconvolution.

    Techniques: Staining, Microscopy