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mtt-dye reduction assay data  (GraphPad Software Inc)


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    Structured Review

    GraphPad Software Inc mtt-dye reduction assay data
    Cytoprotective effects of the tested compounds’ formulation against NMDA-induced excitotoxicity in SH-SY5Y human neuronal cells, as assessed by the <t> MTT-dye reduction assay </t> after 72 h incubation.
    Mtt Dye Reduction Assay Data, supplied by GraphPad Software Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/data+reduction+software/mtt+dye+reduction+assay+data/pmc06837653-335-75-87
    Average 90 stars, based on 1 article reviews
    mtt-dye reduction assay data - by Bioz Stars, 2026-11
    90/100 stars

    Images

    1) Product Images from "3-methoxy aroylhydrazones – free radicals scavenging, anticancer and cytoprotective potency"

    Article Title: 3-methoxy aroylhydrazones – free radicals scavenging, anticancer and cytoprotective potency

    Journal: Redox Report : Communications in Free Radical Research

    doi: 10.1080/13510002.2016.1276256

    Cytoprotective effects of the tested compounds’ formulation against NMDA-induced excitotoxicity in SH-SY5Y human neuronal cells, as assessed by the  MTT-dye reduction assay  after 72 h incubation.
    Figure Legend Snippet: Cytoprotective effects of the tested compounds’ formulation against NMDA-induced excitotoxicity in SH-SY5Y human neuronal cells, as assessed by the MTT-dye reduction assay after 72 h incubation.

    Techniques Used: Formulation, MTT Reduction Assay, Incubation, Control

    Related Articles

    Activity Assay:

    Article Title: 3-methoxy aroylhydrazones – free radicals scavenging, anticancer and cytoprotective potency
    Article Snippet: Their activity decreases in the following order – 3mSIH>3mSBH>3mShBH. table ft1 table-wrap mode="anchored" t5 Table 2. caption a7 Compound IC 50 (μmol/L) Hep-G2 SH-SY-5Y HEK-293 3mSBH 5.59 ± 0.026 22.2 ± 1.7 39.9 ± 3.1 3mShBH 13.21 ± 0.037 >50.0 69.69 ± 2.9 3mSIH 3.678 ± 0.037 21.42 ± 2.6 44.01 ± 3.2 Open in a separate window The concentrations (I C 50 ) causing 50% suppression of cellular viability were calculated from the experimental MTT-dye reduction assay data transformed to sigmoidal dose–response curvesusing non-linear regression analysis (GraphPad Prizm software). caption a8 Cytotoxic activity of the tested hydrazones on HEP-G2 (hepatocellular carcinoma cell line), HEK-293 (human embryotic kidney cell line) and SH-SY-5Y (human neuroblastoma cells).

    MTT Assay:

    Article Title: 3-methoxy aroylhydrazones – free radicals scavenging, anticancer and cytoprotective potency
    Article Snippet: Their activity decreases in the following order – 3mSIH>3mSBH>3mShBH. table ft1 table-wrap mode="anchored" t5 Table 2. caption a7 Compound IC 50 (μmol/L) Hep-G2 SH-SY-5Y HEK-293 3mSBH 5.59 ± 0.026 22.2 ± 1.7 39.9 ± 3.1 3mShBH 13.21 ± 0.037 >50.0 69.69 ± 2.9 3mSIH 3.678 ± 0.037 21.42 ± 2.6 44.01 ± 3.2 Open in a separate window The concentrations (I C 50 ) causing 50% suppression of cellular viability were calculated from the experimental MTT-dye reduction assay data transformed to sigmoidal dose–response curvesusing non-linear regression analysis (GraphPad Prizm software). caption a8 Cytotoxic activity of the tested hydrazones on HEP-G2 (hepatocellular carcinoma cell line), HEK-293 (human embryotic kidney cell line) and SH-SY-5Y (human neuroblastoma cells).

    Transformation Assay:

    Article Title: 3-methoxy aroylhydrazones – free radicals scavenging, anticancer and cytoprotective potency
    Article Snippet: Their activity decreases in the following order – 3mSIH>3mSBH>3mShBH. table ft1 table-wrap mode="anchored" t5 Table 2. caption a7 Compound IC 50 (μmol/L) Hep-G2 SH-SY-5Y HEK-293 3mSBH 5.59 ± 0.026 22.2 ± 1.7 39.9 ± 3.1 3mShBH 13.21 ± 0.037 >50.0 69.69 ± 2.9 3mSIH 3.678 ± 0.037 21.42 ± 2.6 44.01 ± 3.2 Open in a separate window The concentrations (I C 50 ) causing 50% suppression of cellular viability were calculated from the experimental MTT-dye reduction assay data transformed to sigmoidal dose–response curvesusing non-linear regression analysis (GraphPad Prizm software). caption a8 Cytotoxic activity of the tested hydrazones on HEP-G2 (hepatocellular carcinoma cell line), HEK-293 (human embryotic kidney cell line) and SH-SY-5Y (human neuroblastoma cells).

    Software:

    Article Title: 3-methoxy aroylhydrazones – free radicals scavenging, anticancer and cytoprotective potency
    Article Snippet: Their activity decreases in the following order – 3mSIH>3mSBH>3mShBH. table ft1 table-wrap mode="anchored" t5 Table 2. caption a7 Compound IC 50 (μmol/L) Hep-G2 SH-SY-5Y HEK-293 3mSBH 5.59 ± 0.026 22.2 ± 1.7 39.9 ± 3.1 3mShBH 13.21 ± 0.037 >50.0 69.69 ± 2.9 3mSIH 3.678 ± 0.037 21.42 ± 2.6 44.01 ± 3.2 Open in a separate window The concentrations (I C 50 ) causing 50% suppression of cellular viability were calculated from the experimental MTT-dye reduction assay data transformed to sigmoidal dose–response curvesusing non-linear regression analysis (GraphPad Prizm software). caption a8 Cytotoxic activity of the tested hydrazones on HEP-G2 (hepatocellular carcinoma cell line), HEK-293 (human embryotic kidney cell line) and SH-SY-5Y (human neuroblastoma cells).

    Formulation:

    Article Title: 3-methoxy aroylhydrazones – free radicals scavenging, anticancer and cytoprotective potency
    Article Snippet: Their activity decreases in the following order – 3mSIH>3mSBH>3mShBH. table ft1 table-wrap mode="anchored" t5 Table 2. caption a7 Compound IC 50 (μmol/L) Hep-G2 SH-SY-5Y HEK-293 3mSBH 5.59 ± 0.026 22.2 ± 1.7 39.9 ± 3.1 3mShBH 13.21 ± 0.037 >50.0 69.69 ± 2.9 3mSIH 3.678 ± 0.037 21.42 ± 2.6 44.01 ± 3.2 Open in a separate window The concentrations (I C 50 ) causing 50% suppression of cellular viability were calculated from the experimental MTT-dye reduction assay data transformed to sigmoidal dose–response curvesusing non-linear regression analysis (GraphPad Prizm software). caption a8 Cytotoxic activity of the tested hydrazones on HEP-G2 (hepatocellular carcinoma cell line), HEK-293 (human embryotic kidney cell line) and SH-SY-5Y (human neuroblastoma cells).

    MTT Reduction Assay:

    Article Title: 3-methoxy aroylhydrazones – free radicals scavenging, anticancer and cytoprotective potency
    Article Snippet: Their activity decreases in the following order – 3mSIH>3mSBH>3mShBH. table ft1 table-wrap mode="anchored" t5 Table 2. caption a7 Compound IC 50 (μmol/L) Hep-G2 SH-SY-5Y HEK-293 3mSBH 5.59 ± 0.026 22.2 ± 1.7 39.9 ± 3.1 3mShBH 13.21 ± 0.037 >50.0 69.69 ± 2.9 3mSIH 3.678 ± 0.037 21.42 ± 2.6 44.01 ± 3.2 Open in a separate window The concentrations (I C 50 ) causing 50% suppression of cellular viability were calculated from the experimental MTT-dye reduction assay data transformed to sigmoidal dose–response curvesusing non-linear regression analysis (GraphPad Prizm software). caption a8 Cytotoxic activity of the tested hydrazones on HEP-G2 (hepatocellular carcinoma cell line), HEK-293 (human embryotic kidney cell line) and SH-SY-5Y (human neuroblastoma cells).

    Incubation:

    Article Title: 3-methoxy aroylhydrazones – free radicals scavenging, anticancer and cytoprotective potency
    Article Snippet: Their activity decreases in the following order – 3mSIH>3mSBH>3mShBH. table ft1 table-wrap mode="anchored" t5 Table 2. caption a7 Compound IC 50 (μmol/L) Hep-G2 SH-SY-5Y HEK-293 3mSBH 5.59 ± 0.026 22.2 ± 1.7 39.9 ± 3.1 3mShBH 13.21 ± 0.037 >50.0 69.69 ± 2.9 3mSIH 3.678 ± 0.037 21.42 ± 2.6 44.01 ± 3.2 Open in a separate window The concentrations (I C 50 ) causing 50% suppression of cellular viability were calculated from the experimental MTT-dye reduction assay data transformed to sigmoidal dose–response curvesusing non-linear regression analysis (GraphPad Prizm software). caption a8 Cytotoxic activity of the tested hydrazones on HEP-G2 (hepatocellular carcinoma cell line), HEK-293 (human embryotic kidney cell line) and SH-SY-5Y (human neuroblastoma cells).

    Control:

    Article Title: 3-methoxy aroylhydrazones – free radicals scavenging, anticancer and cytoprotective potency
    Article Snippet: Their activity decreases in the following order – 3mSIH>3mSBH>3mShBH. table ft1 table-wrap mode="anchored" t5 Table 2. caption a7 Compound IC 50 (μmol/L) Hep-G2 SH-SY-5Y HEK-293 3mSBH 5.59 ± 0.026 22.2 ± 1.7 39.9 ± 3.1 3mShBH 13.21 ± 0.037 >50.0 69.69 ± 2.9 3mSIH 3.678 ± 0.037 21.42 ± 2.6 44.01 ± 3.2 Open in a separate window The concentrations (I C 50 ) causing 50% suppression of cellular viability were calculated from the experimental MTT-dye reduction assay data transformed to sigmoidal dose–response curvesusing non-linear regression analysis (GraphPad Prizm software). caption a8 Cytotoxic activity of the tested hydrazones on HEP-G2 (hepatocellular carcinoma cell line), HEK-293 (human embryotic kidney cell line) and SH-SY-5Y (human neuroblastoma cells).



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    Image Search Results


    SAXS  data  collection and refinement statistics

    Journal: Communications Biology

    Article Title: Peripheral membrane protein endophilin B1 probes, perturbs and permeabilizes lipid bilayers

    doi: 10.1038/s42003-025-07610-1

    Figure Lengend Snippet: SAXS data collection and refinement statistics

    Article Snippet: (c) Software employed for SAS data reduction, analysis and interpretation , , , , .

    Techniques: Sequencing, Solvent, Concentration Assay, Comparison, Software