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mtt-dye reduction assay data
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Article Title: 3-methoxy aroylhydrazones – free radicals scavenging, anticancer and cytoprotective potency
Journal: Redox Report : Communications in Free Radical Research
doi: 10.1080/13510002.2016.1276256
Figure Legend Snippet: Cytoprotective effects of the tested compounds’ formulation against NMDA-induced excitotoxicity in SH-SY5Y human neuronal cells, as assessed by the MTT-dye reduction assay after 72 h incubation.
Techniques Used: Formulation, MTT Reduction Assay, Incubation, Control
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Activity Assay:Article Title: 3-methoxy aroylhydrazones – free radicals scavenging, anticancer and cytoprotective potency
Article Snippet: Their activity decreases in the following order – 3mSIH>3mSBH>3mShBH. table ft1 table-wrap mode="anchored" t5 Table 2. caption a7 Compound IC 50 (μmol/L) Hep-G2 SH-SY-5Y HEK-293 3mSBH 5.59 ± 0.026 22.2 ± 1.7 39.9 ± 3.1 3mShBH 13.21 ± 0.037 >50.0 69.69 ± 2.9 3mSIH 3.678 ± 0.037 21.42 ± 2.6 44.01 ± 3.2 Open in a separate window The concentrations (I C 50 ) causing 50% suppression of cellular viability were calculated from the experimental MTT-dye reduction assay data transformed to sigmoidal dose–response curvesusing non-linear regression analysis (GraphPad Prizm software). caption a8 Cytotoxic activity of the tested hydrazones on HEP-G2 (hepatocellular carcinoma cell line), HEK-293 (human embryotic kidney cell line) and SH-SY-5Y (human neuroblastoma cells).
MTT Assay:Article Title: 3-methoxy aroylhydrazones – free radicals scavenging, anticancer and cytoprotective potency
Article Snippet: Their activity decreases in the following order – 3mSIH>3mSBH>3mShBH. table ft1 table-wrap mode="anchored" t5 Table 2. caption a7 Compound IC 50 (μmol/L) Hep-G2 SH-SY-5Y HEK-293 3mSBH 5.59 ± 0.026 22.2 ± 1.7 39.9 ± 3.1 3mShBH 13.21 ± 0.037 >50.0 69.69 ± 2.9 3mSIH 3.678 ± 0.037 21.42 ± 2.6 44.01 ± 3.2 Open in a separate window The concentrations (I C 50 ) causing 50% suppression of cellular viability were calculated from the experimental MTT-dye reduction assay data transformed to sigmoidal dose–response curvesusing non-linear regression analysis (GraphPad Prizm software). caption a8 Cytotoxic activity of the tested hydrazones on HEP-G2 (hepatocellular carcinoma cell line), HEK-293 (human embryotic kidney cell line) and SH-SY-5Y (human neuroblastoma cells).
Transformation Assay:Article Title: 3-methoxy aroylhydrazones – free radicals scavenging, anticancer and cytoprotective potency
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Software:Article Title: 3-methoxy aroylhydrazones – free radicals scavenging, anticancer and cytoprotective potency
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Formulation:Article Title: 3-methoxy aroylhydrazones – free radicals scavenging, anticancer and cytoprotective potency
Article Snippet: Their activity decreases in the following order – 3mSIH>3mSBH>3mShBH. table ft1 table-wrap mode="anchored" t5 Table 2. caption a7 Compound IC 50 (μmol/L) Hep-G2 SH-SY-5Y HEK-293 3mSBH 5.59 ± 0.026 22.2 ± 1.7 39.9 ± 3.1 3mShBH 13.21 ± 0.037 >50.0 69.69 ± 2.9 3mSIH 3.678 ± 0.037 21.42 ± 2.6 44.01 ± 3.2 Open in a separate window The concentrations (I C 50 ) causing 50% suppression of cellular viability were calculated from the experimental MTT-dye reduction assay data transformed to sigmoidal dose–response curvesusing non-linear regression analysis (GraphPad Prizm software). caption a8 Cytotoxic activity of the tested hydrazones on HEP-G2 (hepatocellular carcinoma cell line), HEK-293 (human embryotic kidney cell line) and SH-SY-5Y (human neuroblastoma cells).
MTT Reduction Assay:Article Title: 3-methoxy aroylhydrazones – free radicals scavenging, anticancer and cytoprotective potency
Article Snippet: Their activity decreases in the following order – 3mSIH>3mSBH>3mShBH. table ft1 table-wrap mode="anchored" t5 Table 2. caption a7 Compound IC 50 (μmol/L) Hep-G2 SH-SY-5Y HEK-293 3mSBH 5.59 ± 0.026 22.2 ± 1.7 39.9 ± 3.1 3mShBH 13.21 ± 0.037 >50.0 69.69 ± 2.9 3mSIH 3.678 ± 0.037 21.42 ± 2.6 44.01 ± 3.2 Open in a separate window The concentrations (I C 50 ) causing 50% suppression of cellular viability were calculated from the experimental MTT-dye reduction assay data transformed to sigmoidal dose–response curvesusing non-linear regression analysis (GraphPad Prizm software). caption a8 Cytotoxic activity of the tested hydrazones on HEP-G2 (hepatocellular carcinoma cell line), HEK-293 (human embryotic kidney cell line) and SH-SY-5Y (human neuroblastoma cells).
Incubation:Article Title: 3-methoxy aroylhydrazones – free radicals scavenging, anticancer and cytoprotective potency
Article Snippet: Their activity decreases in the following order – 3mSIH>3mSBH>3mShBH. table ft1 table-wrap mode="anchored" t5 Table 2. caption a7 Compound IC 50 (μmol/L) Hep-G2 SH-SY-5Y HEK-293 3mSBH 5.59 ± 0.026 22.2 ± 1.7 39.9 ± 3.1 3mShBH 13.21 ± 0.037 >50.0 69.69 ± 2.9 3mSIH 3.678 ± 0.037 21.42 ± 2.6 44.01 ± 3.2 Open in a separate window The concentrations (I C 50 ) causing 50% suppression of cellular viability were calculated from the experimental MTT-dye reduction assay data transformed to sigmoidal dose–response curvesusing non-linear regression analysis (GraphPad Prizm software). caption a8 Cytotoxic activity of the tested hydrazones on HEP-G2 (hepatocellular carcinoma cell line), HEK-293 (human embryotic kidney cell line) and SH-SY-5Y (human neuroblastoma cells).
Control:Article Title: 3-methoxy aroylhydrazones – free radicals scavenging, anticancer and cytoprotective potency
Article Snippet: Their activity decreases in the following order – 3mSIH>3mSBH>3mShBH. table ft1 table-wrap mode="anchored" t5 Table 2. caption a7 Compound IC 50 (μmol/L) Hep-G2 SH-SY-5Y HEK-293 3mSBH 5.59 ± 0.026 22.2 ± 1.7 39.9 ± 3.1 3mShBH 13.21 ± 0.037 >50.0 69.69 ± 2.9 3mSIH 3.678 ± 0.037 21.42 ± 2.6 44.01 ± 3.2 Open in a separate window The concentrations (I C 50 ) causing 50% suppression of cellular viability were calculated from the experimental MTT-dye reduction assay data transformed to sigmoidal dose–response curvesusing non-linear regression analysis (GraphPad Prizm software). caption a8 Cytotoxic activity of the tested hydrazones on HEP-G2 (hepatocellular carcinoma cell line), HEK-293 (human embryotic kidney cell line) and SH-SY-5Y (human neuroblastoma cells).
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