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idh1 protein  (Proteintech)


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    Structured Review

    Proteintech idh1 protein
    Idh1 Protein, supplied by Proteintech, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/data+processing+software+idps/IDH1+Fusion+Protein/ppr0915689-104-12-25
    Average 92 stars, based on 1 article reviews
    idh1 protein - by Bioz Stars, 2026-09
    92/100 stars

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    Related Articles

    In Vitro:

    Article Title: Scutellarin activates IDH1 to exert antitumor effects in hepatocellular carcinoma progression
    Article Snippet: Finally, the relative IDH1 activity was assessed using a Cytoplasmic Isocitrate Dehydrogenase (ICDHc) Activity Detection Kit (Solarbio) according to the manufacturer’s instructions. .. To detect the direct effect of Scu on the IDH1 protein in vitro, antibody plates were generated by coating the IDH1 antibody (Proteintech) onto 96-well plates (100 ng/well) overnight at 4 °C. ..

    Generated:

    Article Title: Scutellarin activates IDH1 to exert antitumor effects in hepatocellular carcinoma progression
    Article Snippet: Finally, the relative IDH1 activity was assessed using a Cytoplasmic Isocitrate Dehydrogenase (ICDHc) Activity Detection Kit (Solarbio) according to the manufacturer’s instructions. .. To detect the direct effect of Scu on the IDH1 protein in vitro, antibody plates were generated by coating the IDH1 antibody (Proteintech) onto 96-well plates (100 ng/well) overnight at 4 °C. ..

    Article Title: The novel target, mechanism and agonist of α-Ketoglutaric acid in delaying mesenchymal stem cell senescence
    Article Snippet: .. IDH1 activity assay To detect the direct effect of Scu on the IDH1 protein in MSCs, IDH1 antibody plates were generated by coating IDH1 antibody (Proteintech, Wuhan, China) onto 96-well plates (100 ng/well) overnight at 4°C. ..

    Activity Assay:

    Article Title: The novel target, mechanism and agonist of α-Ketoglutaric acid in delaying mesenchymal stem cell senescence
    Article Snippet: .. IDH1 activity assay To detect the direct effect of Scu on the IDH1 protein in MSCs, IDH1 antibody plates were generated by coating IDH1 antibody (Proteintech, Wuhan, China) onto 96-well plates (100 ng/well) overnight at 4°C. ..



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    Image Search Results


    a , Simultaneous in vivo calcium imaging and optogenetic stimulation under free-moving conditions. AAV-syn-GCaMP6f and AAV-syn-ChrimsonR were co-injected into the DG, followed by GRIN-lens implantation. An integrated miniature microscope (nVoke; Inscopix) enabled large-scale cellular-resolution Ca 2+ imaging and optogenetic stimulation. b, Left: Expression of GCaMP6f and ChrimsonR in the DG. Right: Max projection of relative fluorescence change (Δ F / F ) of Ca 2+ transients from all imaging frames during the first 30 min, showing representative active neurons. c, Top: Experimental design of Ca 2+ imaging combined with optogenetic stimulation during the open field test. Each 50-min session consisted of 30-min Ca 2+ imaging (used for the following analysis), 5-min optogenetic stimulation, and 15-min post-stimulation recording. This was repeated for 10 days. No light was introduced to mice in the No Stim group. Bottom: Δ F / F traces from 15 representative neurons (scale bar, 10% Δ F / F ). Data from 25 to 38 min of a 50-min session are shown. d, Distance traveled during the first 30 min e, Average Ca 2+ transient rate during the first 30 min. f, Distribution of spatial information for the No Stim (grey curve) and Stim×10 groups (red curve). The vertical axis represents the frequency of distribution, with the dotted line indicating the criterion for place cells (top 95% percentile of the shuffled distribution; see Methods section). Bar graph indicates proportion of place cells. g, Representative results of position decoding using Ca 2+ imaging data. The first 30 min was split into two 15-min halves for training and test data for decoding. Black/red lines: observed position; grey/pink lines: decoded position. h, Decoding accuracy (mae; mean absolute error, cm). Dotted lines: shuffled control. Two-way repeated measures ANOVA: Stim type, F (1, 8) = 6.49, P = 0.034; Day, F (2, 16) = 2.89, P = 0.085; Stim type × Day, F (2, 16) = 1.11, P = 0.353. Bonferroni correction for multiple comparisons was performed, * P < 0.05. i, Same as f , but for speed information and speed cells. The dotted line indicates the criterion for speed cells (top 99% percentile of the shuffled distribution). j, Same as g , but for speed decoding. k, Same as h , but for speed decoding accuracy. Two-way repeated measures ANOVA: Stim type, F (1, 8) =4.02, P = 0.080; Day, F (2, 16) = 4.87, P = 0.022; Day×Stim type, F (2, 16) = 3.98, P = 0.039. Bonferroni correction for multiple comparisons was performed, * P < 0.05.

    Journal: bioRxiv

    Article Title: Repetitive Neuronal Activation Regulates Cellular Maturation State via Nuclear Reprogramming

    doi: 10.1101/2025.05.02.651848

    Figure Lengend Snippet: a , Simultaneous in vivo calcium imaging and optogenetic stimulation under free-moving conditions. AAV-syn-GCaMP6f and AAV-syn-ChrimsonR were co-injected into the DG, followed by GRIN-lens implantation. An integrated miniature microscope (nVoke; Inscopix) enabled large-scale cellular-resolution Ca 2+ imaging and optogenetic stimulation. b, Left: Expression of GCaMP6f and ChrimsonR in the DG. Right: Max projection of relative fluorescence change (Δ F / F ) of Ca 2+ transients from all imaging frames during the first 30 min, showing representative active neurons. c, Top: Experimental design of Ca 2+ imaging combined with optogenetic stimulation during the open field test. Each 50-min session consisted of 30-min Ca 2+ imaging (used for the following analysis), 5-min optogenetic stimulation, and 15-min post-stimulation recording. This was repeated for 10 days. No light was introduced to mice in the No Stim group. Bottom: Δ F / F traces from 15 representative neurons (scale bar, 10% Δ F / F ). Data from 25 to 38 min of a 50-min session are shown. d, Distance traveled during the first 30 min e, Average Ca 2+ transient rate during the first 30 min. f, Distribution of spatial information for the No Stim (grey curve) and Stim×10 groups (red curve). The vertical axis represents the frequency of distribution, with the dotted line indicating the criterion for place cells (top 95% percentile of the shuffled distribution; see Methods section). Bar graph indicates proportion of place cells. g, Representative results of position decoding using Ca 2+ imaging data. The first 30 min was split into two 15-min halves for training and test data for decoding. Black/red lines: observed position; grey/pink lines: decoded position. h, Decoding accuracy (mae; mean absolute error, cm). Dotted lines: shuffled control. Two-way repeated measures ANOVA: Stim type, F (1, 8) = 6.49, P = 0.034; Day, F (2, 16) = 2.89, P = 0.085; Stim type × Day, F (2, 16) = 1.11, P = 0.353. Bonferroni correction for multiple comparisons was performed, * P < 0.05. i, Same as f , but for speed information and speed cells. The dotted line indicates the criterion for speed cells (top 99% percentile of the shuffled distribution). j, Same as g , but for speed decoding. k, Same as h , but for speed decoding accuracy. Two-way repeated measures ANOVA: Stim type, F (1, 8) =4.02, P = 0.080; Day, F (2, 16) = 4.87, P = 0.022; Day×Stim type, F (2, 16) = 3.98, P = 0.039. Bonferroni correction for multiple comparisons was performed, * P < 0.05.

    Article Snippet: To extract the activity patterns of individual DG neurons from the obtained fluorescent images, we used Inscopix Data Processing Software (IDPS 1.8.0).

    Techniques: In Vivo, Imaging, Injection, Microscopy, Expressing, Fluorescence, Control