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d416050  (Toronto Research Chemicals)


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    Structured Review

    Toronto Research Chemicals d416050

    D416050, supplied by Toronto Research Chemicals, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/dapi/4%E2%80%99%2C6-Diamidino-2-phenylindole+Dihydrochloride/pmc08666359-28-8-4
    Average 92 stars, based on 1 article reviews
    d416050 - by Bioz Stars, 2026-11
    92/100 stars

    Images

    1) Product Images from "Generation of mixed murine organoids to model cellular interactions"

    Article Title: Generation of mixed murine organoids to model cellular interactions

    Journal: STAR Protocols

    doi: 10.1016/j.xpro.2021.100997


    Figure Legend Snippet:

    Techniques Used: Recombinant, Membrane, Software, Microscopy, Transferring, Polymer

    Related Articles

    other:

    Article Title: Generation of mixed murine organoids to model cellular interactions
    Article Snippet: 4′,6-Diamidino-2-phenylindole Dihydrochloride (DAPI) , Toronto Research Chemicals , D416050; CAS RN: 28718-90-3.

    Recombinant:

    Article Title: A Lamb1Dendra2 mouse model identifies basement-membrane-producing origins and dynamics in PyMT breast tumors.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Beta-Dystroglycan Novocastra Cat# NCL-bDG; RRID: AB_442043 CD31 BD BioSciences Cat# 550247; RRID: AB_393571 Collagen IV Abcam Cat# ab19808; RRID: AB_445160 Dendra2 Antibodies-online Cat# ABIN361314; RRID: AB_10789591 Keratin 8 DSHB Cat# TROMA I; RRID: AB_531826 Keratin 14 BioLegend Cat# 905301; RRID: AB_2565048 Alpha smooth muscle actin (aSMA) Sigma Cat# A5228; RRID: AB_262054 Nidogen-1 Nevus Biologicals Cat# NBP1-97701; RRID: AB_94438 Integrin alpha6 A. Sonnenberg, NKI, Amsterdam, the Netherlands #GoH3 Integrin alpha7 A. Sonnenberg, NKI, Amsterdam, the Netherlands #CA Laminin alpha 2 A. Sonnenberg, NKI, Amsterdam, the Netherlands #4H8-2 Laminin alpha 5 L. Sorokin, University of Muenster, Germany #504 Laminin 332 M. Aumailley, University of Cologne, Germany #R14 Goat anti-mouse IgG2a Alexa Fluor 647 ThermoFisher Cat# A21241; RRID: AB_2535810 Donkey anti-rabbit Alexa Fluor 568 ThermoFisher Cat# A10042; RRID: AB_2534017 Goat anti-rabbit Alexa Fluor 647 ThermoFisher Cat# A21245; RRID: AB_253581 Goat anti-rat Alexa Fluor 568 ThermoFisher Cat# A11077; RRID: AB_2534121 Chicken anti-rat Alexa Fluor 647 ThermoFisher Cat# A21472; RRID: AB_2535875 Chemicals, peptides, and recombinant proteins DMEM/F12 Glutamax ThermoFisher Scientific Cat# 31331 Penicillin/streptomycin ThermoFisher Scientific Cat# 15140-122 HEPES ThermoFisher Scientific Cat# 15630-056 B27 ThermoFisher Scientific Cat# 17504-044 FGF Gibco Cat# PHG0261 Y-27632 Selleckchem Cat# S6390 Cultrex PathClear Reduced Growth Factor Basement Membrane Extract Type 2 R&D Systems Cat# 3533-005-02 TrypLE ThermoFisher Scientific Cat# 12605-010 Collagenase A Roche Cat# 10103586001 DNAseI Roche Cat# 10104159001 Tetramethylrhodamine isothiocyanate–Dextran Sigma Cat# 52194-1G 4,6-Diamidino-2-phenylindole Dihydrochloride (Dapi) Toronto Research Chemicals Cat# D416050 Normal goat serum Gibco Cat# 16210072 Paraformaldehyde 16% (w/v) in aqueous solutionmethanol-free Alfa Aesar Cat# 43368.9M Critical commercial assays DynaExpress Long ssDNA Preparation Kit BioDynamics Laboratory Cat# DS635 MycoAlert PLUS Kit Lonza Cat# LT07-118 (Continued on next page) e1 Developmental Cell 58, 535–549.e1–e5, April 10, 2023 .. REAGENT or RESOURCE SOURCE IDENTIFIER Experimental models: Cell lines PyMT ECad-CFP organoids (pure FVB) This Paper N/A PyMT ECad-CFP Lamb1Dendra2 organoids (pure FVB) This Paper N/A Experimental models: Organisms/strains Mouse: FVB/N-Tg(MMTV-PyVT)634Mul/J Jackson Laboratory JAX: 002374 Mouse: Ecad-mCFP Snippert et al.32 J:164644 Mouse: Lamb1Dendra2 This paper JAX: 038019 Mouse: FVB/NRj Javier labs N/A Oligonucleotides sgRNA msLamb1 This Paper TTACAGCACCTGCTTATAACAGG Lamb1Dendra2 wt fw This Paper AGCTCAAGAATTGGTGCGAC Lamb1Dendra2 mut rev This Paper GGTCAGGATGTCGTAGCTGAA Lamb1Dendra2 wt rev This Paper TTGTAGATGACTGCCTCGGT Software and algorithms Leica LASX Leica RRID:SCR_013673 Fiji https://imagej.net/ RRID:SCR_002285 Imaris Version 9.6.0 Bitplane RRID:SCR_007370 Huygens Software SVI RRID:SCR_014237 GraphPad Prism Version 8 and 9 GraphPad RRID:SCR_002798 Other Betadine Mylan Cat# 01331 Duratears Alcon Cat# 0037820

    Membrane:

    Article Title: A Lamb1Dendra2 mouse model identifies basement-membrane-producing origins and dynamics in PyMT breast tumors.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Beta-Dystroglycan Novocastra Cat# NCL-bDG; RRID: AB_442043 CD31 BD BioSciences Cat# 550247; RRID: AB_393571 Collagen IV Abcam Cat# ab19808; RRID: AB_445160 Dendra2 Antibodies-online Cat# ABIN361314; RRID: AB_10789591 Keratin 8 DSHB Cat# TROMA I; RRID: AB_531826 Keratin 14 BioLegend Cat# 905301; RRID: AB_2565048 Alpha smooth muscle actin (aSMA) Sigma Cat# A5228; RRID: AB_262054 Nidogen-1 Nevus Biologicals Cat# NBP1-97701; RRID: AB_94438 Integrin alpha6 A. Sonnenberg, NKI, Amsterdam, the Netherlands #GoH3 Integrin alpha7 A. Sonnenberg, NKI, Amsterdam, the Netherlands #CA Laminin alpha 2 A. Sonnenberg, NKI, Amsterdam, the Netherlands #4H8-2 Laminin alpha 5 L. Sorokin, University of Muenster, Germany #504 Laminin 332 M. Aumailley, University of Cologne, Germany #R14 Goat anti-mouse IgG2a Alexa Fluor 647 ThermoFisher Cat# A21241; RRID: AB_2535810 Donkey anti-rabbit Alexa Fluor 568 ThermoFisher Cat# A10042; RRID: AB_2534017 Goat anti-rabbit Alexa Fluor 647 ThermoFisher Cat# A21245; RRID: AB_253581 Goat anti-rat Alexa Fluor 568 ThermoFisher Cat# A11077; RRID: AB_2534121 Chicken anti-rat Alexa Fluor 647 ThermoFisher Cat# A21472; RRID: AB_2535875 Chemicals, peptides, and recombinant proteins DMEM/F12 Glutamax ThermoFisher Scientific Cat# 31331 Penicillin/streptomycin ThermoFisher Scientific Cat# 15140-122 HEPES ThermoFisher Scientific Cat# 15630-056 B27 ThermoFisher Scientific Cat# 17504-044 FGF Gibco Cat# PHG0261 Y-27632 Selleckchem Cat# S6390 Cultrex PathClear Reduced Growth Factor Basement Membrane Extract Type 2 R&D Systems Cat# 3533-005-02 TrypLE ThermoFisher Scientific Cat# 12605-010 Collagenase A Roche Cat# 10103586001 DNAseI Roche Cat# 10104159001 Tetramethylrhodamine isothiocyanate–Dextran Sigma Cat# 52194-1G 4,6-Diamidino-2-phenylindole Dihydrochloride (Dapi) Toronto Research Chemicals Cat# D416050 Normal goat serum Gibco Cat# 16210072 Paraformaldehyde 16% (w/v) in aqueous solutionmethanol-free Alfa Aesar Cat# 43368.9M Critical commercial assays DynaExpress Long ssDNA Preparation Kit BioDynamics Laboratory Cat# DS635 MycoAlert PLUS Kit Lonza Cat# LT07-118 (Continued on next page) e1 Developmental Cell 58, 535–549.e1–e5, April 10, 2023 .. REAGENT or RESOURCE SOURCE IDENTIFIER Experimental models: Cell lines PyMT ECad-CFP organoids (pure FVB) This Paper N/A PyMT ECad-CFP Lamb1Dendra2 organoids (pure FVB) This Paper N/A Experimental models: Organisms/strains Mouse: FVB/N-Tg(MMTV-PyVT)634Mul/J Jackson Laboratory JAX: 002374 Mouse: Ecad-mCFP Snippert et al.32 J:164644 Mouse: Lamb1Dendra2 This paper JAX: 038019 Mouse: FVB/NRj Javier labs N/A Oligonucleotides sgRNA msLamb1 This Paper TTACAGCACCTGCTTATAACAGG Lamb1Dendra2 wt fw This Paper AGCTCAAGAATTGGTGCGAC Lamb1Dendra2 mut rev This Paper GGTCAGGATGTCGTAGCTGAA Lamb1Dendra2 wt rev This Paper TTGTAGATGACTGCCTCGGT Software and algorithms Leica LASX Leica RRID:SCR_013673 Fiji https://imagej.net/ RRID:SCR_002285 Imaris Version 9.6.0 Bitplane RRID:SCR_007370 Huygens Software SVI RRID:SCR_014237 GraphPad Prism Version 8 and 9 GraphPad RRID:SCR_002798 Other Betadine Mylan Cat# 01331 Duratears Alcon Cat# 0037820



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