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4 6 diamidino 2 phenylindole dapi staining solution  (Miltenyi Biotec)


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    Structured Review

    Miltenyi Biotec 4 6 diamidino 2 phenylindole dapi staining solution
    4 6 Diamidino 2 Phenylindole Dapi Staining Solution, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 97/100, based on 94 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/dapi/DAPI+Staining+Solution/pmc12346281-237-10-14
    Average 97 stars, based on 94 article reviews
    4 6 diamidino 2 phenylindole dapi staining solution - by Bioz Stars, 2026-10
    97/100 stars

    Images

    Related Articles

    Staining:

    Article Title: Receptor-guided AAV Tropism Engineering via MATCH
    Article Snippet: After 10 minutes, cells were stained with APC anti-human CD3 Antibody (BioLegend, 300412) and FITC anti-human CD25 Antibody (BioLegend, 302603) for 30 minutes on ice. .. Cells were washed once with 1✕ PBS with 2% FBS, pH 7.4, and finally resuspended in 1✕ PBS with 2% FBS and 0.1 μg/mL DAPI Staining Solution (Miltenyi Biotec, 130-111-570), pH 7.4 and analyzed on the BD LSRFortessaTM Cell Analyzer. ..

    Article Title: Cysteine availability tunes ubiquitin signaling via inverse stability of LRRC58 E3 ligase and its substrate CDO1.
    Article Snippet: Analytical flow cytometry was performed on an Attune NxT flow cytometer (Thermo Fisher Scientific) equipped with an automated plate reader. .. Cells were prepared by single-cell dissociation using TrypLE, then resuspended in 1 × DPBS supplemented with 2mM EDTA and DAPI staining solution (Miltenyi Biotec) for live/dead discrimination. ..

    Article Title: An Integrin β6-targeted antibody-drug conjugate optimized for intravesical delivery to treat non-muscle invasive bladder cancer
    Article Snippet: PDOs and cells were dissociated with TrypLE (ThermoFisher, 12605010) for 5-10 min at 37°C, then distributed into 96-well V-bottom plates (2 x 105 cells/well). .. Cells were stained with h2A2 primary antibody (1 hour, 4°C), anti-IgG-FITC (45 minutes, 4°C) and DAPI (Miltenyi Biotec, 130-111-570) (5 minutes). .. Samples were acquired within 4 hours on a MACSQuant Analyzer 10 (Miltenyi Biotec, RRID:SCR_020268) or Attune NxT flow cytometer and analyzed using FlowJo v10.8.

    Article Title: Co-expression of non-signaling CARs enhances EGFRvIII-specific CAR T-cell cytotoxicity against head and neck squamous cell carcinoma.
    Article Snippet: For the detection of CARs or nsCARs on T-cells in the three fractions (pre-MACS, flow-through, and post-MACS), staining was performed using FITC- or PE-conjugated CD34 antibodies (clone QBEND/10, Thermo Fisher Scientific; FITC: MA1-10204, PE: MA1-10205) and APC- or PE-Vio770-conjugated CD271 antibodies (clone ME20.4, Miltenyi Biotec; APC: 130-113-418, PE-Vio770: 130-133-422). .. After staining and washing, cells were resuspended in MACS buffer containing either PI (Thermo Scientific; P1304MP) or DAPI (Miltenyi Biotec; 130-111-570) for live/dead cell discrimination. ..

    Single Cell:

    Article Title: Cysteine availability tunes ubiquitin signaling via inverse stability of LRRC58 E3 ligase and its substrate CDO1.
    Article Snippet: Analytical flow cytometry was performed on an Attune NxT flow cytometer (Thermo Fisher Scientific) equipped with an automated plate reader. .. Cells were prepared by single-cell dissociation using TrypLE, then resuspended in 1 × DPBS supplemented with 2mM EDTA and DAPI staining solution (Miltenyi Biotec) for live/dead discrimination. ..

    Incubation:

    Article Title: Hyaluronic acid and tissue mechanics orchestrate mammalian digit tip regeneration.
    Article Snippet: .. Then, cells were incubated with ready- to- use DAPI (Miltenyi Biotec, 130- 111- 570) for 5 min in 2% FBS and 2.5 mM EDTA in PBS. ..

    Magnetic Cell Separation:

    Article Title: Co-expression of non-signaling CARs enhances EGFRvIII-specific CAR T-cell cytotoxicity against head and neck squamous cell carcinoma.
    Article Snippet: For the detection of CARs or nsCARs on T-cells in the three fractions (pre-MACS, flow-through, and post-MACS), staining was performed using FITC- or PE-conjugated CD34 antibodies (clone QBEND/10, Thermo Fisher Scientific; FITC: MA1-10204, PE: MA1-10205) and APC- or PE-Vio770-conjugated CD271 antibodies (clone ME20.4, Miltenyi Biotec; APC: 130-113-418, PE-Vio770: 130-133-422). .. After staining and washing, cells were resuspended in MACS buffer containing either PI (Thermo Scientific; P1304MP) or DAPI (Miltenyi Biotec; 130-111-570) for live/dead cell discrimination. ..



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    Image Search Results


    ABCA1 and APOE expression is altered in hippocampal astrocytes in KA‐ and pilocarpine‐induced epilepsy models. (A,B) Western blot analysis (A) and quantification of APOE/β‐Tubulin (B) in the mouse hippocampus at 1, 2, 4, and 8 weeks after KA‐induced status epilepticus (SE) ( n = 5 per group). (C,D) Quantification of the percentage of APOE‐expressing astrocytes (APOE + /GFAP + double‐positive, C) and ABCA1/APOE‐co‐expressing astrocytes (ABCA1 + /APOE + /GFAP + triple‐positive, D) relative to total GFAP + cells in the hippocampal hilus at 1 week after KA‐induced SE ( n = 5 per group). (E) Representative immunofluorescence images showing the expression of APOE (red), ABCA1 (green), and GFAP (astrocyte marker, cyan) in the hippocampus at 1 week after KA‐induced SE. Nuclei were counterstained with DAPI (blue). White dashed boxes indicate the regions shown in the magnified insets, and white arrowheads indicate APOE and ABCA1 immunoreactivity associated with GFAP + astrocytic profiles. Scale bars: 20 µm (main panels) and 8 µm (magnified insets). (F–H) Western blot analysis of ABCA1 and APOE expression in the pilocarpine‐induced epilepsy model (F), with quantification of relative ABCA1/GAPDH (G) and APOE/β‐Tubulin (H) protein levels at different time points ( n = 3 per group). (I) Representative multiplex immunofluorescence images showing expression of APOE (red), ABCA1 (green), GFAP (cyan) and DAPI (blue) at 1 week after pilocarpine‐induced SE. White dashed boxes indicate the regions shown in the magnified insets, and white arrowheads indicate APOE and ABCA1 immunoreactivity associated with GFAP + astrocytic profiles. Scale bars: 20 µm (main panels) and 8 µm (magnified insets). Data are presented as mean ± SD. Western blot data were analyzed using two‐way ANOVA followed by Sidak's multiple‐comparisons test. Immunofluorescence data were analyzed using two‐sided unpaired Welch's t‐tests. * p < 0.05, ** p < 0.01, **** p < 0.0001. Numbers above bars indicate exact non‐significant P values.

    Journal: Advanced Science

    Article Title: Astrocyte‐Associated ABCA1/APOE Responses Accompany Hippocampal Lipid Remodeling and Ectopic Neuronal Accumulation During Epileptogenesis

    doi: 10.1002/advs.78329

    Figure Lengend Snippet: ABCA1 and APOE expression is altered in hippocampal astrocytes in KA‐ and pilocarpine‐induced epilepsy models. (A,B) Western blot analysis (A) and quantification of APOE/β‐Tubulin (B) in the mouse hippocampus at 1, 2, 4, and 8 weeks after KA‐induced status epilepticus (SE) ( n = 5 per group). (C,D) Quantification of the percentage of APOE‐expressing astrocytes (APOE + /GFAP + double‐positive, C) and ABCA1/APOE‐co‐expressing astrocytes (ABCA1 + /APOE + /GFAP + triple‐positive, D) relative to total GFAP + cells in the hippocampal hilus at 1 week after KA‐induced SE ( n = 5 per group). (E) Representative immunofluorescence images showing the expression of APOE (red), ABCA1 (green), and GFAP (astrocyte marker, cyan) in the hippocampus at 1 week after KA‐induced SE. Nuclei were counterstained with DAPI (blue). White dashed boxes indicate the regions shown in the magnified insets, and white arrowheads indicate APOE and ABCA1 immunoreactivity associated with GFAP + astrocytic profiles. Scale bars: 20 µm (main panels) and 8 µm (magnified insets). (F–H) Western blot analysis of ABCA1 and APOE expression in the pilocarpine‐induced epilepsy model (F), with quantification of relative ABCA1/GAPDH (G) and APOE/β‐Tubulin (H) protein levels at different time points ( n = 3 per group). (I) Representative multiplex immunofluorescence images showing expression of APOE (red), ABCA1 (green), GFAP (cyan) and DAPI (blue) at 1 week after pilocarpine‐induced SE. White dashed boxes indicate the regions shown in the magnified insets, and white arrowheads indicate APOE and ABCA1 immunoreactivity associated with GFAP + astrocytic profiles. Scale bars: 20 µm (main panels) and 8 µm (magnified insets). Data are presented as mean ± SD. Western blot data were analyzed using two‐way ANOVA followed by Sidak's multiple‐comparisons test. Immunofluorescence data were analyzed using two‐sided unpaired Welch's t‐tests. * p < 0.05, ** p < 0.01, **** p < 0.0001. Numbers above bars indicate exact non‐significant P values.

    Article Snippet: Cell nuclei were subsequently counterstained with DAPI (SBA‐0100‐20, 1:1000, SouthernBiotech).

    Techniques: Expressing, Western Blot, Analysis, Immunofluorescence, Marker, Multiplex Assay