fluorescent blocking agent containing 4’,6-diamidino-2-phenylindole (dapi) (Beyotime)
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fluorescent blocking agent containing 4’,6-diamidino-2-phenylindole (dapi)
Fluorescent Blocking Agent Containing 4’,6 Diamidino 2 Phenylindole (Dapi), supplied by Beyotime, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/dapi-containing+blocking+agent/fluorescent+blocking+agent+containing+4%E2%80%99+6+diamidino+2+phenylindole++dapi+/pmc11228890-74-71-69
Average 90 stars, based on 1 article reviews
Fluorescent Blocking Agent Containing 4’,6 Diamidino 2 Phenylindole (Dapi), supplied by Beyotime, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/dapi-containing+blocking+agent/fluorescent+blocking+agent+containing+4%E2%80%99+6+diamidino+2+phenylindole++dapi+/pmc11228890-74-71-69
Average 90 stars, based on 1 article reviews
fluorescent blocking agent containing 4’,6-diamidino-2-phenylindole (dapi) - by Bioz Stars,
2026-10
90/100 stars
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Incubation:Article Title: Chitosan/PLGA-based tissue engineered nerve grafts with SKP-SC-EVs enhance sciatic nerve regeneration in dogs through miR-30b-5p-mediated regulation of axon growth Article Snippet: The acquired SKP-SCs were seeded on dishes coated with poly- d -lysine (Sigma-Aldrich, St. Louis, MO, USA) and laminin (Corning, Brooklyn, NY, USA) for culture in DMEM/F12 (3:1) medium supplemented with 3 % fetal bovine serum (Gibco, Carlsbad, CA, USA), 2 % N2 supplement (StemCell Technologies, Vancouver, BC, Canada), 5 μM forskolin (Sigma-Aldrich), 50 ng/ml heregulin-1β (R&D Systems, MN, USA), and 1 % penicillin/streptomycin (Beyotime, Shanghai, China). .. After being fixed with 4 % paraformaldehyde and blocked for 1.5 h at room temperature, SKP-SCs were incubated with anti-GFAP (1:500, Abcam, Cambridge, MA, USA) and anti-S100 (1:500, Abcam) at 4 °C overnight, followed by reaction with Alexa Fluor 594-conjugated anti-mouse IgG and Alexa Fluor 488-conjugated anti-rabbit IgG secondary antibody (1:400, Abcam) at 37 °C for 2 h, and the cell nuclei were counterstained with a Blocking Assay:Article Title: Chitosan/PLGA-based tissue engineered nerve grafts with SKP-SC-EVs enhance sciatic nerve regeneration in dogs through miR-30b-5p-mediated regulation of axon growth Article Snippet: The acquired SKP-SCs were seeded on dishes coated with poly- d -lysine (Sigma-Aldrich, St. Louis, MO, USA) and laminin (Corning, Brooklyn, NY, USA) for culture in DMEM/F12 (3:1) medium supplemented with 3 % fetal bovine serum (Gibco, Carlsbad, CA, USA), 2 % N2 supplement (StemCell Technologies, Vancouver, BC, Canada), 5 μM forskolin (Sigma-Aldrich), 50 ng/ml heregulin-1β (R&D Systems, MN, USA), and 1 % penicillin/streptomycin (Beyotime, Shanghai, China). .. After being fixed with 4 % paraformaldehyde and blocked for 1.5 h at room temperature, SKP-SCs were incubated with anti-GFAP (1:500, Abcam, Cambridge, MA, USA) and anti-S100 (1:500, Abcam) at 4 °C overnight, followed by reaction with Alexa Fluor 594-conjugated anti-mouse IgG and Alexa Fluor 488-conjugated anti-rabbit IgG secondary antibody (1:400, Abcam) at 37 °C for 2 h, and the cell nuclei were counterstained with a |