dapi staining solution (Beyotime)

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Beyotime dapi staining solution
Dapi Staining Solution, supplied by Beyotime, used in various techniques. Bioz Stars score: 99/100, based on 2029 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/dapi+staining+solution/DAPI+Staining+Solution/pmc13097096-64-0-21
Average 99 stars, based on 2029 article reviews

dapi staining solution - by Bioz Stars, 2026-09

99/100 stars

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Bicinchoninic Acid Protein Assay:

Article Title: The host protein SSR4 mediates PRRSV-induced endoplasmic reticulum stress via interaction with Nsp2.
Article Snippet: The following primary antibodies were used: anti-β-actin (Proteintech, 66009-1-Ig), anti-Calnexin (Proteintech, 10427-2-AP), anti-SSR1 (Proteintech, 10583-1-AP), anti-SSR2 (Proteintech, 10278-1-AP), anti-SSR3 (Proteintech, 30851-1-AP), anti-SSR4 (Proteintech, 11655-2-AP), anti-ATF4 (Proteintech, 10835-1-AP), anti-EDEM1 (Proteintech, 26226-1-AP), anti-GRP78 (Beyotime, AF0171), anti-ATF6 (Proteintech, 24169-1-AP), anti-Phospho-eIF2 alpha (Cell Signaling Technology, #3398), anti-eIF2 alpha (Cell Signaling Technol ogy, #5324), anti-Phospho-IRE1 (HUABIO, HA721980), anti-IRE1 (HUABIO, HA723225), anti-Puromycin (ABclonal, A21205), monoclonal anti-PRRSV N protein (generated in-house), and monoclonal anti-PRRSV Nsp2 (generated in-house). .. Key commercial reagents included tunicamycin (Beyotime, SC0393), DTT (Solarbio, 3483-12-3), 4-phenyl butyric acid (MCE, HY-A0281), MK-28 (AbMole, M56016), puromycin (MCE, HY-B1743), cycloheximide (MCE, HY-12320), BCA Protein Assay Kit (Thermo Fisher, 23225), Lipo fectamine 3000 (Thermo Fisher, L3000015), Lipofectamine RNAiMAX (Thermo Fisher, 13778075), ReverTra Ace qPCR RT Kit (TOYOBO, NLQ-101), KOD qPCR SYBR Mix (TOYOBO, QKD-201T), tunicamycin (MedChemExpress, HY-A0098), RIPA lysis buffer (Beyotime, P0013C), DAPI staining solution (Beyotime, C1006), and polyethylenimine (PEI; Yeasen). ..

Article Title: Development of perilla essential oil-based nanoemulsions as anti-psoriatic and dermal delivery platforms for topical therapy
Article Snippet: PEG-40 stearyl ether (S40) was obtained from Croda GmbH (Nett et al., Germany). .. DAPI staining solution, RIPA buffer, hematoxylin and eosin (H&E) staining kit, and bicinchoninic acid (BCA) protein assay kits were obtained from Beyotime Biotechnology (Shanghai, China). .. Antibodies against Ki67, TNF-α, IL-6, IL-1β, IL-17 and a one-step TUNEL assay kit were from Servicebio (Wuhan, China).

Real-time Polymerase Chain Reaction:

Lysis:

Staining:

Article Title: A photodynamically activated nanoplatform relieves glucose-driven immunosuppression to potentiate STING immunotherapy in triple-negative breast cancer
Article Snippet: Pre-screened foetal bovine serum (FBP-C520) was procured from Haixing Biosciences. .. DAPI Staining Solution, Cell Viability/Cytotoxicity assay kit, Cell Cycle and Apoptosis analysis kit, Reactive Oxygen Species (ROS) assay kit, and Mitochondrial Membrane Potential assay kit with JC-1 were all purchased from Beyotime, Inc. .. Cell Counting Kit-8 was obtained from TargetMol, USA.

Article Title: Gastrodin alleviates neuronal damage in epileptic cell models by targeting P2RY12 to inhibit microglial hyperactivation
Article Snippet: .. Then, DAPI staining solution (Beyotime, China, C1005, 1 μg/mL) was added, and the cells were incubated for 5 minutes to stain the nuclei. .. After washing with PBS, the images were acquired using a confocal microscope (Zeiss LSM880).

Article Title: Targeted delivery of lupeol via hyaluronic acid-modified ZIF-8 for anti-hepatic fibrosis therapy
Article Snippet: Quantitative analysis was performed using flow cytometry (Beckman Coulter, USA). .. A DAPI Staining Solution (Beyotime Biotechnology, Shanghai, China) was used to perform the cell apoptosis shape assay on LX-2 cells according to the manufacturer's instructions. .. Cells were seeded into six-well plates at a density of 1.0 × 10 6 cells per well and cultured for 24 h. The next day, cells were treated with different media containing Lupeol, Lupeol@ZIF-8 and HA/Lupeol@ZIF-8 (60 μM lupeol), with blank and model groups included as controls, and incubated for an additional 24 h. For qualitative analysis, DAPI solution was added 24 h after treatment, and fluorescence signals were recorded using a fluorescence microscope (Olympus, Tokyo, Japan).

Article Title: Geometry-driven immunomodulation in 3D-printed bioceramics: Negative curvature promotes macrophage M2 polarization via Ras-MAPK/HIF-1α signaling for vascularized osteogenesis
Article Snippet: The cells types utilized in this study included: hBMSCs (No. 7500, ScienCell, USA.), Raw264.7 (CL-0190, Procell system, China), HUVECs (No. 8000, ScienCell, USA.), rBMSCs (MUBMX-01001, OriCell, USA.) and MS1(CL-0162, Procell system, China). .. The reagents used in the experiment included: H-DMEM(11965118, Gibco, USA.), α-DMEM medium(12571063, Gibco, USA.), TritonX-100(ST1723, Beyotime, China), 4 % paraformaldehyde (BL539A, Biosharp, China),FBS(A5256701, Gibco, USA.),ECM medium (Science Cell, USA.),and DAPI staining solution (C1006, Beyotime, China),BCIP/NBT(C3206, Beyotime, China), reactive oxygen species kit (S0033S, Beyotime, China), BSA (B2064, ≥98 %, Sigma-Aldrich, USA.),CD31 antibody (ab28364, Abcam, USA.), secondary anti-IGg (ab175773, Alexa Fluor® 680, Abcam, USA.), Phalloidin-iFluor 488(ab176753, Abcam, USA.), CCR7(AF5293, Bioss, China), CD206 (bsm-60761R, Bioss, China), iNOS (bs-22924R, Bioss, China), RIPA (P0013, Beyotime, China), p-ERK1/2 (AF3687, Affinity, USA.) and ERK1/2 (#AF0155, Affinity, USA), luminol detection reagent (sc-2048, Santa Cruz, USA.), GAPDH (Cat#KC-5G5, Kangchen Biotechnology, China), Trizol(15596026CN, Invitrogen, USA.), DEPC(R0601, Thermo Scientific, USA.), TBST(R017R.0000, Thermo Scientific, USA.), HIF-1a(GTX127309, GeneTex, USA.), β-Tubulin(10094-1-AP, Proteintech, UK) Adezmapimod (SB 203580, MCE, USA.) medium and Paclitaxel (99.88 %, HY-B0015R, MCE), ELISA Arg-1(E-EL-M3092, ELabSci@, China), TNF-α (E-EL-M3063, ELabSci@, China), OPN(22952-1-AP, Proteintech, UK.), F4/80 (GB11027-100, Servicebio, China) Alkaline phosphatase activity kit (P0321S, Beyotime, China), Matrigel (CLS356234, Corning, USA), Microfill MV120 (Flow tech, USA), EDTA(17892, Thermo Scientific, USA.) xylene(X112051, AR,99 %, Aladdin, China), ethanol (107-21-1, AR,99 %, Aladdin, China). .. All primers were designed and synthesized by Genema (Shanghai) Co., Ltd. 5.2.Fabrication of high-throughput array cell culture chip (HCGC) with Gaussian curvature gradient distribution. (1) Constructing models with different Gaussian curvatures: the multipipe of Grasshopper in Rhinocero software is used to regulate Gaussian curvature of the truss in the diamond framework in .

Article Title: Activation of the TGR5/cAMP/PKA/CREB axis in cholangiocytes mediates epithelial-mesenchymal transition and fibrosis in hepatolithiasis.
Article Snippet: On the following day, cells were washed three times with PBS (5 minutes each), then 313 incubated at 37°C for 30 minutes with fluorescent secondary antibodies (e.g., Alexa Fluor 488/594 314 conjugates; Invitrogen, 1:500). .. After additional PBS washes, DAPI staining solution (Beyotime, 315 Cat# C1002, 1 μg/mL) was added and incubated in the dark for 3 minutes. ..

Article Title: Gastrodin alleviates neuronal damage in epileptic cell models by targeting P2RY12 to inhibit microglial hyperactivation.
Article Snippet: .. Then, DAPI staining solution (Beyotime, China, C1005, 1 μg/mL) was added, and the cells were incubated for 5 minutes to stain the nuclei. .. After washing with PBS, the images were acquired using a confocal microscope (Zeiss LSM880).

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dapi staining solution (Beyotime)

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Bicinchoninic Acid Protein Assay:

Real-time Polymerase Chain Reaction:

Lysis:

Staining:

ROS Assay:

Membrane:

Incubation:

Enzyme-linked Immunosorbent Assay:

Activity Assay:

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