dapi solution (Applichem inc)

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Applichem inc dapi solution

Confocal scanning microscope (20 μm scale bar, 20× magnification) image of the immunocytochemistry analysis of HDF cells to evaluate collagenase Type I and elastin expressions. Untreated control cells stain relatively weakly when stained with antibodies against collagen Type I and elastin than cells that have been treated with the hybrosome. <t>DAPI-stained</t> nuclei appear blue. Three independent experiments were repeated for immunocytochemistry studies and only 1 result image added for each group. <t>DAPI,</t> <t>diamidino-2-phenylindole;</t> HDF, human dermal fibroblast cell line.

Dapi Solution, supplied by Applichem inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/dapi+staining+solution+4/dapi+solution/pmc10195566-61-8-11
Average 90 stars, based on 1 article reviews

dapi solution - by Bioz Stars, 2026-11

90/100 stars

Images

1) Product Images from "The Effect of Hybrosome (Umbilical Cord Blood Exosome–Liposome Hybrid Vesicles) on Human Dermal Cells In Vitro"

Article Title: The Effect of Hybrosome (Umbilical Cord Blood Exosome–Liposome Hybrid Vesicles) on Human Dermal Cells In Vitro

Journal: Aesthetic Surgery Journal. Open Forum

doi: 10.1093/asjof/ojad039

Figure Legend Snippet: Confocal scanning microscope (20 μm scale bar, 20× magnification) image of the immunocytochemistry analysis of HDF cells to evaluate collagenase Type I and elastin expressions. Untreated control cells stain relatively weakly when stained with antibodies against collagen Type I and elastin than cells that have been treated with the hybrosome. DAPI-stained nuclei appear blue. Three independent experiments were repeated for immunocytochemistry studies and only 1 result image added for each group. DAPI, diamidino-2-phenylindole; HDF, human dermal fibroblast cell line.

Techniques Used: Microscopy, Immunocytochemistry, Control, Staining

other:

Article Title: Pentameric assembly of glycine receptor intracellular domains provides insights into gephyrin clustering
Article Snippet: Nuclei were stained with DAPI (1:1000 in PBS, AppliChem, Darmstadt, Germany) and coverslips were mounted on Mowiol 4-88 (Calbiochem, Merck Millipore, Billerica, Massachussetts) with the addition of 2,5% 1,4-Diazobicyclo[2.2.2]octan (Dabco, Merck Millipore), adjusted to pH 9.0.

Article Title: Crocetin Prevents RPE Cells from Oxidative Stress through Protection of Cellular Metabolic Function and Activation of ERK1/2
Article Snippet: After treatment, ARPE19 cells were washed 3× 5 min with PBS, fixed with 4% Paraformaldehyde for 20 min at room temperature, washed 3× 5 min with PBS and stained with DAPI (1/15000, AppliChem, Darmstadt, Germany) at 4 °C for 12 h. After washing three times with PBS (5 min), the nuclei were visualized and the number of healthy and pyknotic nuclei were quantified using the Operetta high-content imaging system (PerKinElmer, Waltham, MA, USA).

Article Title: Monocyte recruitment to the inflamed central nervous system: migration pathways and distinct functional polarization
Article Snippet: Slices were incubated with DAPI (1:5000, 1mg/ml stock, AppliChem, Darmstadt, Germany), diluted in 1x TBS for 20 min at RT.

Suspension:

Article Title: Control of Expression of Key Cell Cycle Enzymes Drives Cell Line-Specific Functions of CDK7 in Human PDAC Cells
Article Snippet: .. For cell cycle analyses, 100 μL of cell suspension were diluted with 900μL DAPI solution (3 μM; Applichem, Darmstadt, Germany) and cells stained for 15 min at room temperature. ..

Staining:

Article Title: The Effect of Hybrosome (Umbilical Cord Blood Exosome–Liposome Hybrid Vesicles) on Human Dermal Cells In Vitro
Article Snippet: Cells were then incubated with 0.4% goat anti-mouse AlexaFluor-647 antibody (Abcam) for 1 h at 4°C and washed gently. .. For nuclei examination, cells were stained with 0.1% DAPI solution (4′,6-di-di-amidino-2-phenylindole; AppliChem, Darmstadt, Germany) for 20 min at 4°C and washed with PBS-T. .. Cells were observed using a confocal laser scanning microscope (LSM800; Carl Zeiss, Oberkochen, Germany).

Article Title: The Chromatin Extrusion Phenomenon in Amoeba proteus Cell Cycle.
Article Snippet: Amoeba proteus is possibly the best known of all unicellular eukaryotes.. At the same time, several quintessential issues of its biology, including some aspects of the cell cycle, remain unsolved.. Here, we show that this obligate agamic amoebae and related species have a special type of cyclic polyploidy.

Incubation:

Article Title: Supporting Information for A Genome-Wide CRISPR/Cas9 Screen Identifies a Role for Rab5A and Early Endosomes in Hepatitis E Virus Replication
Article Snippet: .. Following three washing steps with PBS, cells were incubated 5 min with DAPI solution (AppliChem, Darmstadt, Germany) for nuclei counterstaining. .. Finally, coverslips were mounted on glass slides using the ProLong Antifade Mountant (Thermo Fisher Scientific) and examined using a Zeiss LSM 900 Airyscan 2 confocal laser scanning microscope.

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