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dapi solution  (FUJIFILM)

 
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    FUJIFILM dapi solution
    Dapi Solution, supplied by FUJIFILM, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/dapi+staining+solution+4/dapi+solution/pm37584382-142-4-9
    Average 90 stars, based on 1 article reviews
    dapi solution - by Bioz Stars, 2026-10
    90/100 stars

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    other:

    Article Title: Complete suspension culture of human induced pluripotent stem cells supplemented with suppressors of spontaneous differentiation
    Article Snippet: Chemical compound, drug , DAPI solution , FUJIFILM Wako Pure Chemical Corporation , Cat#340-07971 , .

    Staining:

    Article Title: Macrophage-like Blood Cells Are Involved in Inter-Tissue Communication to Activate JAK/STAT Signaling, Inducing Antitumor Turandot Proteins in Drosophila Fat Body via the TNF-JNK Pathway
    Article Snippet: .. After staining with DAPI solution (#5748, FUJIFILM Wako Pure Chemical, Osaka, Japan), the fixed LG samples embedded in Vector Shield (#H-1000, Vector Laboratories, Newark, CA, USA) were gently flattened under a covered glass to prepare specimens of a constant thickness, as described in [ ]. .. Specimens were observed under a fluorescence microscope (I×81; Olympus Co., Tokyo, Japan) equipped with a CCD camera (ORCA-R2; Hamamatsu Photonics, Hamamatsu, Japan).

    Article Title: The minimal endosymbiosis between Spurilla braziliana (Aeolidiidae, Nudibranchia) and Symbiodiniaceae
    Article Snippet: After washing with PBS, the samples were cleared using the CUBIC Trial Kit (FUJIFILM Wako Pure Chemical Corporation), according to the protocol provided by the manufacturer. .. During the clearing process, nuclear staining was performed using DAPI solution (FUJIFILM Wako Pure Chemical Corporation). .. Chlorophyll autofluorescence from Symbiodiniaceae and DAPI fluorescence were observed under a fluorescence microscope (KEYENCE BZX-810).

    Article Title: Macrophage-like Blood Cells Are Involved in Inter-Tissue Communication to Activate JAK/STAT Signaling, Inducing Antitumor Turandot Proteins in Drosophila Fat Body via the TNF-JNK Pathway.
    Article Snippet: .. After staining with DAPI solution (#5748, FUJIFILM Wako Pure Chemical, Osaka, Japan), the fixed LG samples embedded in Vector Shield (#H-1000, Vector Laboratories, Newark, CA, USA) were gently flattened under a covered glass to prepare specimens of a constant thickness, as described in [7]. .. Specimens were observed under a fluorescence microscope (I×81; Olympus Co., Tokyo, Japan) equipped with a CCD Int.

    Binding Assay:

    Article Title: Complete suspension culture of human induced pluripotent stem cells supplemented with suppressors of spontaneous differentiation
    Article Snippet: .. The dissociated cells were aliquoted into 1 × 105 cells/100 μL with a binding buffer consisting of 10 mM HEPES, 140 mM NaCl, 2.5 mM CaCl2, then added with 5 μL Annexin V (Alexa Fluor 680) conjugates (Cat#A35109, Thermo Fisher Scientific) and 1 μL DAPI solution (Cat#340- 07971, FUJIFILM Wako Pure Chemical Corporation). ..

    Real-time Polymerase Chain Reaction:

    Article Title: Complete suspension culture of human induced pluripotent stem cells supplemented with suppressors of spontaneous differentiation
    Article Snippet: .. or resource Designation Source or reference Identifiers Additional information Commercial assay or kit FastGene RNA premium kit NIPPON Genetics Cat#FG- 81250 Commercial assay or kit ReverTra Ace qPCR RT kit TOYOBO Cat#FSQ- 101 Commercial assay or kit THUNDERBIRD Probe qPCR Mix TOYOBO Cat#QPS- 101 Commercial assay or kit DNeasy Blood & Tissue Kit QIAGEN Cat#69504 Commercial assay or kit Karyostat Assay arrays Thermo Fisher Scientific Cat#905403 Chemical compound, drug Y- 27632 FUJIFILM Wako Pure Chemical Corporation Cat# HY- 10071 Chemical compound, drug 0.5 M EDTA solution Nacalai Tesque Cat#06894- 14 Chemical compound, drug Annexin V (Alexa Fluor 680) conjugates Thermo Fisher Scientific Cat#A35109 Chemical compound, drug DAPI solution FUJIFILM Wako Pure Chemical Corporation Cat340- 07971 Chemical compound, drug 4% paraformaldehyde in phosphate buffer solution Nacalai Tesque Cat#09154- 85 Chemical compound, drug SB431542 FUJIFILM Wako Pure Chemical Corporation Cat#198- 16543 (10 μM) Chemical compound, drug DMH1 FUJIFILM Wako Pure Chemical Corporation Cat#041- 33881 (10 μM) Continued .. In this study, we used WTC11 (GM25256 from Coriell Institute) (Hayashi et al., 2016), 201B7 (HPS0063 from RIKEN Cell Bank) (Takahashi et al., 2007), 454E2 (HPS0077 from RIKEN Cell Bank) (Okita et al., 2011), 1383D6 (HPS1006 from RIKEN Cell Bank) (Okita et al., 2011), 1231A3 (HPS0381 from RIKEN Cell Bank) (Okita et al., 2011), Ff- I14s04 (CiRA foundation, Kyoto University) (Kitano et al., 2022), and HiPS- NB1RGB (HPS5067 from RIKEN Cell Bank), which were generated from human neonatal skin fibroblast (RCB0222) (Borisova et al., 2022; Shimizu et al., 2022), as healthy donor hiPSC lines.



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    LF-PVA@Pro NPs alleviate CRS-induced neuropathological damage and restore neuronal activity and neurotransmitter-related signaling in the VTA and NAc. ( A ) Representative H&E staining images of the ventral tegmental area and nucleus accumbens from the control, CRS, free-propofol, PVA@Pro NPs and LF-PVA@Pro NPs groups. Boxed regions are shown at higher magnification. ( B ) Quantification of histopathological injury scores in the VTA and NAc. ( C ) Representative Nissl-staining images of the VTA and NAc in the indicated groups, with enlarged views of the selected regions. ( D) Quantification of morphologically normal Nissl-positive neurons in the VTA and NAc. ( E ) Representative immunofluorescence images of c-Fos&GABA and c-Fos&DAT staining in the VTA. Nuclei were <t>counterstained</t> with <t>DAPI.</t> ( F ) Quantification of c-Fos and GABA fluorescence intensities in the VTA. ( G ) Quantification of c-Fos and DAT fluorescence intensities in the VTA. ( H ) Representative immunofluorescence images of c-Fos&GABA and c-Fos&DAT staining in the NAc. ( I ) Quantification of c-Fos and GABA fluorescence intensities in the NAc. ( J ) Quantification of c-Fos and DAT fluorescence intensities in the NAc. CRS exposure caused marked histopathological injury, loss of Nissl-positive neurons, and reductions in c-Fos-, GABA-, and DAT-associated fluorescence signals in the VTA and NAc. LF-PVA@Pro NPs treatment attenuated tissue injury and produced greater recovery of neuronal integrity and functional marker expression than free propofol. Data are presented as the mean ± SD (n=3). *p <0.05, p <0.01, ***p <0.001 , and ****p <0.0001 ; ns, not significant.
    Dapi Staining Solution, supplied by Boster Bio, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    LF-PVA@Pro NPs alleviate CRS-induced neuropathological damage and restore neuronal activity and neurotransmitter-related signaling in the VTA and NAc. ( A ) Representative H&E staining images of the ventral tegmental area and nucleus accumbens from the control, CRS, free-propofol, PVA@Pro NPs and LF-PVA@Pro NPs groups. Boxed regions are shown at higher magnification. ( B ) Quantification of histopathological injury scores in the VTA and NAc. ( C ) Representative Nissl-staining images of the VTA and NAc in the indicated groups, with enlarged views of the selected regions. ( D) Quantification of morphologically normal Nissl-positive neurons in the VTA and NAc. ( E ) Representative immunofluorescence images of c-Fos&GABA and c-Fos&DAT staining in the VTA. Nuclei were <t>counterstained</t> with <t>DAPI.</t> ( F ) Quantification of c-Fos and GABA fluorescence intensities in the VTA. ( G ) Quantification of c-Fos and DAT fluorescence intensities in the VTA. ( H ) Representative immunofluorescence images of c-Fos&GABA and c-Fos&DAT staining in the NAc. ( I ) Quantification of c-Fos and GABA fluorescence intensities in the NAc. ( J ) Quantification of c-Fos and DAT fluorescence intensities in the NAc. CRS exposure caused marked histopathological injury, loss of Nissl-positive neurons, and reductions in c-Fos-, GABA-, and DAT-associated fluorescence signals in the VTA and NAc. LF-PVA@Pro NPs treatment attenuated tissue injury and produced greater recovery of neuronal integrity and functional marker expression than free propofol. Data are presented as the mean ± SD (n=3). *p <0.05, p <0.01, ***p <0.001 , and ****p <0.0001 ; ns, not significant.
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    LF-PVA@Pro NPs alleviate CRS-induced neuropathological damage and restore neuronal activity and neurotransmitter-related signaling in the VTA and NAc. ( A ) Representative H&E staining images of the ventral tegmental area and nucleus accumbens from the control, CRS, free-propofol, PVA@Pro NPs and LF-PVA@Pro NPs groups. Boxed regions are shown at higher magnification. ( B ) Quantification of histopathological injury scores in the VTA and NAc. ( C ) Representative Nissl-staining images of the VTA and NAc in the indicated groups, with enlarged views of the selected regions. ( D) Quantification of morphologically normal Nissl-positive neurons in the VTA and NAc. ( E ) Representative immunofluorescence images of c-Fos&GABA and c-Fos&DAT staining in the VTA. Nuclei were <t>counterstained</t> with <t>DAPI.</t> ( F ) Quantification of c-Fos and GABA fluorescence intensities in the VTA. ( G ) Quantification of c-Fos and DAT fluorescence intensities in the VTA. ( H ) Representative immunofluorescence images of c-Fos&GABA and c-Fos&DAT staining in the NAc. ( I ) Quantification of c-Fos and GABA fluorescence intensities in the NAc. ( J ) Quantification of c-Fos and DAT fluorescence intensities in the NAc. CRS exposure caused marked histopathological injury, loss of Nissl-positive neurons, and reductions in c-Fos-, GABA-, and DAT-associated fluorescence signals in the VTA and NAc. LF-PVA@Pro NPs treatment attenuated tissue injury and produced greater recovery of neuronal integrity and functional marker expression than free propofol. Data are presented as the mean ± SD (n=3). *p <0.05, p <0.01, ***p <0.001 , and ****p <0.0001 ; ns, not significant.
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    Image Search Results


    LF-PVA@Pro NPs alleviate CRS-induced neuropathological damage and restore neuronal activity and neurotransmitter-related signaling in the VTA and NAc. ( A ) Representative H&E staining images of the ventral tegmental area and nucleus accumbens from the control, CRS, free-propofol, PVA@Pro NPs and LF-PVA@Pro NPs groups. Boxed regions are shown at higher magnification. ( B ) Quantification of histopathological injury scores in the VTA and NAc. ( C ) Representative Nissl-staining images of the VTA and NAc in the indicated groups, with enlarged views of the selected regions. ( D) Quantification of morphologically normal Nissl-positive neurons in the VTA and NAc. ( E ) Representative immunofluorescence images of c-Fos&GABA and c-Fos&DAT staining in the VTA. Nuclei were counterstained with DAPI. ( F ) Quantification of c-Fos and GABA fluorescence intensities in the VTA. ( G ) Quantification of c-Fos and DAT fluorescence intensities in the VTA. ( H ) Representative immunofluorescence images of c-Fos&GABA and c-Fos&DAT staining in the NAc. ( I ) Quantification of c-Fos and GABA fluorescence intensities in the NAc. ( J ) Quantification of c-Fos and DAT fluorescence intensities in the NAc. CRS exposure caused marked histopathological injury, loss of Nissl-positive neurons, and reductions in c-Fos-, GABA-, and DAT-associated fluorescence signals in the VTA and NAc. LF-PVA@Pro NPs treatment attenuated tissue injury and produced greater recovery of neuronal integrity and functional marker expression than free propofol. Data are presented as the mean ± SD (n=3). *p <0.05, p <0.01, ***p <0.001 , and ****p <0.0001 ; ns, not significant.

    Journal: International Journal of Nanomedicine

    Article Title: Lactoferrin-Modified Ultra-Sustained Release Propofol Nanoparticles for Multi-Pathway Therapy of Depression via Intraperitoneal Injection

    doi: 10.2147/IJN.S645818

    Figure Lengend Snippet: LF-PVA@Pro NPs alleviate CRS-induced neuropathological damage and restore neuronal activity and neurotransmitter-related signaling in the VTA and NAc. ( A ) Representative H&E staining images of the ventral tegmental area and nucleus accumbens from the control, CRS, free-propofol, PVA@Pro NPs and LF-PVA@Pro NPs groups. Boxed regions are shown at higher magnification. ( B ) Quantification of histopathological injury scores in the VTA and NAc. ( C ) Representative Nissl-staining images of the VTA and NAc in the indicated groups, with enlarged views of the selected regions. ( D) Quantification of morphologically normal Nissl-positive neurons in the VTA and NAc. ( E ) Representative immunofluorescence images of c-Fos&GABA and c-Fos&DAT staining in the VTA. Nuclei were counterstained with DAPI. ( F ) Quantification of c-Fos and GABA fluorescence intensities in the VTA. ( G ) Quantification of c-Fos and DAT fluorescence intensities in the VTA. ( H ) Representative immunofluorescence images of c-Fos&GABA and c-Fos&DAT staining in the NAc. ( I ) Quantification of c-Fos and GABA fluorescence intensities in the NAc. ( J ) Quantification of c-Fos and DAT fluorescence intensities in the NAc. CRS exposure caused marked histopathological injury, loss of Nissl-positive neurons, and reductions in c-Fos-, GABA-, and DAT-associated fluorescence signals in the VTA and NAc. LF-PVA@Pro NPs treatment attenuated tissue injury and produced greater recovery of neuronal integrity and functional marker expression than free propofol. Data are presented as the mean ± SD (n=3). *p <0.05, p <0.01, ***p <0.001 , and ****p <0.0001 ; ns, not significant.

    Article Snippet: The sections were subsequently counterstained with DAPI staining solution (Boster Biological Technology Co., Ltd.) for 15 min.

    Techniques: Activity Assay, Staining, Control, Immunofluorescence, Fluorescence, Tissue, Produced, Functional Assay, Marker, Expressing