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Sangon Biotech dapi staining solution
Dapi Staining Solution, supplied by Sangon Biotech, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/dapi+solution/dapi+solution+staining/pm42123883-145-2-5
Average 86 stars, based on 1 article reviews
dapi staining solution - by Bioz Stars, 2026-10
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Related Articles

Staining:

Article Title: Enterohemorrhagic Escherichia coli senses colonic oxygen availability to modulate its virulence and colonization
Article Snippet: .. DAPI Stain Solution , Sangon Biotech , Cat#E607303. .. Actin-Tracker Green-488 , Beyotime , Cat#C2221S.

Article Title: An Immune Microenvironment-Integrated Intestinal-on-a-Chip Model for Investigating Immunopathogenesis in Inflammatory Bowel Disease.
Article Snippet: .. Finally, add DAPI staining solution (Sangon Biotech, Shanghai, China) and incubate in the dark for 5 min to stain cell nuclei. ..

Article Title: The calcium ion channel protein TRPML is indispensable to the reproduction of Nilaparvata lugens and the transovarial transmission of its yeast-like symbionts.
Article Snippet: The secondary antibody was a goat anti-rabbit IgG antibody conjugated with Dylight 488 fluorescent dye (Abbkine, Atlanta, GA, USA). .. Nuclei were stained with DAPI staining solution (Sangon Biotech, Shanghai, China). .. Samples were observed using a laser scanning confocal microscope (SP8; Leica, Mannheim, Germany).

Article Title: Harnessing the yeast nucleus for the efficient production of valuable monoterpenes.
Article Snippet: Subcellular engineering emerges as a pivotal strategy to amplify terpene production in yeast cell factories.. The yeast nucleus, which shares acetyl-CoA pool with the cytosol and maintains nuclear membrane integrity during mitosis, offers unique compartmentalization capabilities.. Despite these attributes, there is a notable absence of research harnessing the potential of the yeast nucleus for subcellular engineering.

Article Title: An Immune Microenvironment-Integrated Intestinal-on-a-Chip Model for Investigating Immunopathogenesis in Inflammatory Bowel Disease
Article Snippet: .. Finally, add DAPI staining solution (Sangon Biotech, Shanghai, China) and incubate in the dark for 5 min to stain cell nuclei. ..

Article Title: TRIM47 Regulates Energy Metabolism via Glycolytic Reprogramming to Drive Hepatocellular Carcinoma Progression and Represents an Efficient Therapeutic Target
Article Snippet: Meanwhile, experimental cells were seeded in confocal dishes (Thermo Fisher Scientific, USA) at a density of 20%–30% and cultured overnight in a sugar‐free medium. .. The cells were subsequently incubated in glucose‐free DMEM containing 20 μ m 2‐NBDG (Thermo Fisher Scientific, USA) for 2 h. After being washed with PBS, the cells were fixed with 4% paraformaldehyde, and a DAPI working solution (Sangon Biotech, China) was added to stain the cell nuclei for 5 min. .. The stained sections were visualized via a confocal microscope (Zeiss, Germany).

Article Title: Rapid detection of Streptococcus agalactiae in tilapia using RPA-PfAgo detection systems
Article Snippet: Streptococcus agalactiae (GBS), a leading tilapia pathogen, causes high mortality with frequent asymptomatic infections, complicating early detection.. The study focused on GBS and employed recombinase polymerase amplification (RPA) technology in conjunction with Pyrococcus furiosus Argonaute (PfAgo) to establish real-time fluorescent RPA-PfAgo (RPA-PfAgo-RTF) and lateral flow strip-based RPA-PfAgo assay (RPA-PfAgo-LFD).. The conserved cfb gene was selected for the design and screening of primers, gDNAs, and molecular beacons.

other:

Article Title: Deciphering OCT4A-dose-dependent transcriptional profiles associated with tumorigenic potential in somatic cancer cells.
Article Snippet: Thereafter, cells were incubated with anti-OCT4A (Cell Signaling Technology 2890S) at 4°C overnight.

Incubation:

Article Title: TRIM47 Regulates Energy Metabolism via Glycolytic Reprogramming to Drive Hepatocellular Carcinoma Progression and Represents an Efficient Therapeutic Target
Article Snippet: Meanwhile, experimental cells were seeded in confocal dishes (Thermo Fisher Scientific, USA) at a density of 20%–30% and cultured overnight in a sugar‐free medium. .. The cells were subsequently incubated in glucose‐free DMEM containing 20 μ m 2‐NBDG (Thermo Fisher Scientific, USA) for 2 h. After being washed with PBS, the cells were fixed with 4% paraformaldehyde, and a DAPI working solution (Sangon Biotech, China) was added to stain the cell nuclei for 5 min. .. The stained sections were visualized via a confocal microscope (Zeiss, Germany).

Polymerase Chain Reaction:

Article Title: Rapid detection of Streptococcus agalactiae in tilapia using RPA-PfAgo detection systems
Article Snippet: Streptococcus agalactiae (GBS), a leading tilapia pathogen, causes high mortality with frequent asymptomatic infections, complicating early detection.. The study focused on GBS and employed recombinase polymerase amplification (RPA) technology in conjunction with Pyrococcus furiosus Argonaute (PfAgo) to establish real-time fluorescent RPA-PfAgo (RPA-PfAgo-RTF) and lateral flow strip-based RPA-PfAgo assay (RPA-PfAgo-LFD).. The conserved cfb gene was selected for the design and screening of primers, gDNAs, and molecular beacons.

Plasmid Preparation:

Article Title: Rapid detection of Streptococcus agalactiae in tilapia using RPA-PfAgo detection systems
Article Snippet: Streptococcus agalactiae (GBS), a leading tilapia pathogen, causes high mortality with frequent asymptomatic infections, complicating early detection.. The study focused on GBS and employed recombinase polymerase amplification (RPA) technology in conjunction with Pyrococcus furiosus Argonaute (PfAgo) to establish real-time fluorescent RPA-PfAgo (RPA-PfAgo-RTF) and lateral flow strip-based RPA-PfAgo assay (RPA-PfAgo-LFD).. The conserved cfb gene was selected for the design and screening of primers, gDNAs, and molecular beacons.

DNA Extraction:

Article Title: Rapid detection of Streptococcus agalactiae in tilapia using RPA-PfAgo detection systems
Article Snippet: Streptococcus agalactiae (GBS), a leading tilapia pathogen, causes high mortality with frequent asymptomatic infections, complicating early detection.. The study focused on GBS and employed recombinase polymerase amplification (RPA) technology in conjunction with Pyrococcus furiosus Argonaute (PfAgo) to establish real-time fluorescent RPA-PfAgo (RPA-PfAgo-RTF) and lateral flow strip-based RPA-PfAgo assay (RPA-PfAgo-LFD).. The conserved cfb gene was selected for the design and screening of primers, gDNAs, and molecular beacons.



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Image Search Results


Journal: iScience

Article Title: A multimodal in situ framework for spatial characterization applied on SARS-CoV-2 live-attenuated vaccines in the nasal mucosa

doi: 10.1016/j.isci.2026.117730

Figure Lengend Snippet:

Article Snippet: Finally, sections were counterstained with Diamidin-2-phenylindol (DAPI, ACD Bio-Techne GmbH) for 30 seconds at RT and mounted directly using ProLong Gold antifade mounting medium (Thermo Fisher Scientific, USA).

Techniques: Plasmid Preparation, Virus, Indirect ELISA, Recombinant, RNAscope, Multiplex Assay, Software, Negative Control, Confocal, Laser-Scanning Microscopy

LF-PVA@Pro NPs alleviate CRS-induced neuropathological damage and restore neuronal activity and neurotransmitter-related signaling in the VTA and NAc. ( A ) Representative H&E staining images of the ventral tegmental area and nucleus accumbens from the control, CRS, free-propofol, PVA@Pro NPs and LF-PVA@Pro NPs groups. Boxed regions are shown at higher magnification. ( B ) Quantification of histopathological injury scores in the VTA and NAc. ( C ) Representative Nissl-staining images of the VTA and NAc in the indicated groups, with enlarged views of the selected regions. ( D) Quantification of morphologically normal Nissl-positive neurons in the VTA and NAc. ( E ) Representative immunofluorescence images of c-Fos&GABA and c-Fos&DAT staining in the VTA. Nuclei were counterstained with DAPI. ( F ) Quantification of c-Fos and GABA fluorescence intensities in the VTA. ( G ) Quantification of c-Fos and DAT fluorescence intensities in the VTA. ( H ) Representative immunofluorescence images of c-Fos&GABA and c-Fos&DAT staining in the NAc. ( I ) Quantification of c-Fos and GABA fluorescence intensities in the NAc. ( J ) Quantification of c-Fos and DAT fluorescence intensities in the NAc. CRS exposure caused marked histopathological injury, loss of Nissl-positive neurons, and reductions in c-Fos-, GABA-, and DAT-associated fluorescence signals in the VTA and NAc. LF-PVA@Pro NPs treatment attenuated tissue injury and produced greater recovery of neuronal integrity and functional marker expression than free propofol. Data are presented as the mean ± SD (n=3). *p <0.05, p <0.01, ***p <0.001 , and ****p <0.0001 ; ns, not significant.

Journal: International Journal of Nanomedicine

Article Title: Lactoferrin-Modified Ultra-Sustained Release Propofol Nanoparticles for Multi-Pathway Therapy of Depression via Intraperitoneal Injection

doi: 10.2147/IJN.S645818

Figure Lengend Snippet: LF-PVA@Pro NPs alleviate CRS-induced neuropathological damage and restore neuronal activity and neurotransmitter-related signaling in the VTA and NAc. ( A ) Representative H&E staining images of the ventral tegmental area and nucleus accumbens from the control, CRS, free-propofol, PVA@Pro NPs and LF-PVA@Pro NPs groups. Boxed regions are shown at higher magnification. ( B ) Quantification of histopathological injury scores in the VTA and NAc. ( C ) Representative Nissl-staining images of the VTA and NAc in the indicated groups, with enlarged views of the selected regions. ( D) Quantification of morphologically normal Nissl-positive neurons in the VTA and NAc. ( E ) Representative immunofluorescence images of c-Fos&GABA and c-Fos&DAT staining in the VTA. Nuclei were counterstained with DAPI. ( F ) Quantification of c-Fos and GABA fluorescence intensities in the VTA. ( G ) Quantification of c-Fos and DAT fluorescence intensities in the VTA. ( H ) Representative immunofluorescence images of c-Fos&GABA and c-Fos&DAT staining in the NAc. ( I ) Quantification of c-Fos and GABA fluorescence intensities in the NAc. ( J ) Quantification of c-Fos and DAT fluorescence intensities in the NAc. CRS exposure caused marked histopathological injury, loss of Nissl-positive neurons, and reductions in c-Fos-, GABA-, and DAT-associated fluorescence signals in the VTA and NAc. LF-PVA@Pro NPs treatment attenuated tissue injury and produced greater recovery of neuronal integrity and functional marker expression than free propofol. Data are presented as the mean ± SD (n=3). *p <0.05, p <0.01, ***p <0.001 , and ****p <0.0001 ; ns, not significant.

Article Snippet: The sections were subsequently counterstained with DAPI staining solution (Boster Biological Technology Co., Ltd.) for 15 min.

Techniques: Activity Assay, Staining, Control, Immunofluorescence, Fluorescence, Tissue, Produced, Functional Assay, Marker, Expressing