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Servicebio Inc dapi dye solution
Dapi Dye Solution, supplied by Servicebio Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/dapi+dye+solution/dapi+solution/pmc12897916-74-3-7
Average 86 stars, based on 1 article reviews
dapi dye solution - by Bioz Stars, 2026-09
86/100 stars

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Related Articles

Staining:

Article Title: Mechanism of ferroptosis in progressive injury of skeletal muscle caused by high-voltage electrical burns and the intervention effect of uAMC3203.
Article Snippet: .. Subsequently, DAPI staining solution (Servicebio, Wuhan, China) was applied, and the sections were incubated at room temperature in the dark for 10 min. A spontaneous fluorescence quenching agent (Servicebio, Wuhan, China) was added for 5 min. After rinsing, images were captured using a microscope (Nikon, Tokyo, Japan). ..

Article Title: Recombinant tissue plasminogen activator protects neurons after intracerebral hemorrhage through activating the PI3K/AKT/mTOR pathway
Article Snippet: After washing with PBS, the cells were incubated with Cy3-labeled Goat Anti-Rabbit IgG(H+L) secondary antibody (1:100, Beyotime, Cat# A0516, RRID: AB_2893015) for 1 hour at room temperature. .. Next, 10 μL of DAPI staining solution (Servicebio) was added to the coverslips and cells were incubated for 5 minutes at room temperature. .. After washing with PBS three times, a drop of anti-fluorescent quench sealer (Servicebio) was placed in the center of the coverslips.

Article Title: Evolutionary conservation and divergence of phagocytic and coagulation programs across bilaterian circulating immune cells.
Article Snippet: .. After being washed by the following SSC: 2 × SSC, 37°C 3 for 10 min, 1 × SSC, 37°C for 2 × 5 min, 0.5 × SSC 37°C for 10 min, DAPI staining solution 4 (Servicebio, China) was added to the cell slices in the dark condition for 8 min. ..

Article Title: Exercise Alleviates Osteoporosis and Hyperglycemia in Type 1 Diabetes Mellitus Mice via Piezo1-Mediated Mechanotransduction.
Article Snippet: .. Nuclei were subsequently stained with DAPI solution (Servicebio, Wuhan, China) for 15 min. Fluorescence images were captured using an inverted microscope (Olympus IX73, Tokyo, Japan). ..

Article Title: Therapeutic potential of ADAR1-regulated macrophage exosomes for improving myocardial damage in septic cardiomyopathy
Article Snippet: To determine the mitochondrial membrane potential, a JC-1 mitochondrial membrane potential detection kit (Beyotime Biotechnology, China) was used. .. HL-1 cells were seeded on sterile coverslips, cultured to the logarithmic phase, washed twice with prewarmed 1× PBS (Solarbio, Cat. No. P1010), and incubated with 1 mL of freshly prepared JC-1 staining working solution at 37 °C and 5% CO 2 in the dark for 20 min. After staining, the cells were washed twice with 1× JC-1 staining buffer, incubated with DAPI solution (G1012-100ML; Servicebio, China) at room temperature in the dark for 10 min for nuclear staining, mounted with anti-fluorescence quenching medium, and observed under a fluorescence microscope (red fluorescence for normal mitochondrial membrane potential, green fluorescence for decreased potential). ..

Article Title: Bangia fusco-purpurea polysaccharides attenuate DSS-induced colitis in mice through gut microbiota and its metabolism modulation
Article Snippet: The next day, CY3-labeled goat anti-rabbit IgG (1:300) secondary antibody (Servicebio, Wuhan, China) was added and incubated in the dark at room temperature for 50 min. .. Subsequently, the sections were incubated with DAPI staining solution (Servicebio, Wuhan, China) in the dark at room temperature for 10 min, followed by the addition of autofluorescence quencher (G1221) (Servicebio, Wuhan, China) for 5 min and rinsing. .. Finally, the sections were covered with antifluorescence quencher mounting medium and images were obtained using an inverted fluorescence microscope (Nikon Eclipse C1, Tokyo, Japan).

Incubation:

Article Title: Mechanism of ferroptosis in progressive injury of skeletal muscle caused by high-voltage electrical burns and the intervention effect of uAMC3203.
Article Snippet: .. Subsequently, DAPI staining solution (Servicebio, Wuhan, China) was applied, and the sections were incubated at room temperature in the dark for 10 min. A spontaneous fluorescence quenching agent (Servicebio, Wuhan, China) was added for 5 min. After rinsing, images were captured using a microscope (Nikon, Tokyo, Japan). ..

Article Title: Recombinant tissue plasminogen activator protects neurons after intracerebral hemorrhage through activating the PI3K/AKT/mTOR pathway
Article Snippet: After washing with PBS, the cells were incubated with Cy3-labeled Goat Anti-Rabbit IgG(H+L) secondary antibody (1:100, Beyotime, Cat# A0516, RRID: AB_2893015) for 1 hour at room temperature. .. Next, 10 μL of DAPI staining solution (Servicebio) was added to the coverslips and cells were incubated for 5 minutes at room temperature. .. After washing with PBS three times, a drop of anti-fluorescent quench sealer (Servicebio) was placed in the center of the coverslips.

Article Title: Therapeutic potential of ADAR1-regulated macrophage exosomes for improving myocardial damage in septic cardiomyopathy
Article Snippet: To determine the mitochondrial membrane potential, a JC-1 mitochondrial membrane potential detection kit (Beyotime Biotechnology, China) was used. .. HL-1 cells were seeded on sterile coverslips, cultured to the logarithmic phase, washed twice with prewarmed 1× PBS (Solarbio, Cat. No. P1010), and incubated with 1 mL of freshly prepared JC-1 staining working solution at 37 °C and 5% CO 2 in the dark for 20 min. After staining, the cells were washed twice with 1× JC-1 staining buffer, incubated with DAPI solution (G1012-100ML; Servicebio, China) at room temperature in the dark for 10 min for nuclear staining, mounted with anti-fluorescence quenching medium, and observed under a fluorescence microscope (red fluorescence for normal mitochondrial membrane potential, green fluorescence for decreased potential). ..

Article Title: Bangia fusco-purpurea polysaccharides attenuate DSS-induced colitis in mice through gut microbiota and its metabolism modulation
Article Snippet: The next day, CY3-labeled goat anti-rabbit IgG (1:300) secondary antibody (Servicebio, Wuhan, China) was added and incubated in the dark at room temperature for 50 min. .. Subsequently, the sections were incubated with DAPI staining solution (Servicebio, Wuhan, China) in the dark at room temperature for 10 min, followed by the addition of autofluorescence quencher (G1221) (Servicebio, Wuhan, China) for 5 min and rinsing. .. Finally, the sections were covered with antifluorescence quencher mounting medium and images were obtained using an inverted fluorescence microscope (Nikon Eclipse C1, Tokyo, Japan).

Fluorescence:

Article Title: Mechanism of ferroptosis in progressive injury of skeletal muscle caused by high-voltage electrical burns and the intervention effect of uAMC3203.
Article Snippet: .. Subsequently, DAPI staining solution (Servicebio, Wuhan, China) was applied, and the sections were incubated at room temperature in the dark for 10 min. A spontaneous fluorescence quenching agent (Servicebio, Wuhan, China) was added for 5 min. After rinsing, images were captured using a microscope (Nikon, Tokyo, Japan). ..

Article Title: Exercise Alleviates Osteoporosis and Hyperglycemia in Type 1 Diabetes Mellitus Mice via Piezo1-Mediated Mechanotransduction.
Article Snippet: .. Nuclei were subsequently stained with DAPI solution (Servicebio, Wuhan, China) for 15 min. Fluorescence images were captured using an inverted microscope (Olympus IX73, Tokyo, Japan). ..

Article Title: Therapeutic potential of ADAR1-regulated macrophage exosomes for improving myocardial damage in septic cardiomyopathy
Article Snippet: To determine the mitochondrial membrane potential, a JC-1 mitochondrial membrane potential detection kit (Beyotime Biotechnology, China) was used. .. HL-1 cells were seeded on sterile coverslips, cultured to the logarithmic phase, washed twice with prewarmed 1× PBS (Solarbio, Cat. No. P1010), and incubated with 1 mL of freshly prepared JC-1 staining working solution at 37 °C and 5% CO 2 in the dark for 20 min. After staining, the cells were washed twice with 1× JC-1 staining buffer, incubated with DAPI solution (G1012-100ML; Servicebio, China) at room temperature in the dark for 10 min for nuclear staining, mounted with anti-fluorescence quenching medium, and observed under a fluorescence microscope (red fluorescence for normal mitochondrial membrane potential, green fluorescence for decreased potential). ..

Microscopy:

Article Title: Mechanism of ferroptosis in progressive injury of skeletal muscle caused by high-voltage electrical burns and the intervention effect of uAMC3203.
Article Snippet: .. Subsequently, DAPI staining solution (Servicebio, Wuhan, China) was applied, and the sections were incubated at room temperature in the dark for 10 min. A spontaneous fluorescence quenching agent (Servicebio, Wuhan, China) was added for 5 min. After rinsing, images were captured using a microscope (Nikon, Tokyo, Japan). ..

Article Title: Therapeutic potential of ADAR1-regulated macrophage exosomes for improving myocardial damage in septic cardiomyopathy
Article Snippet: To determine the mitochondrial membrane potential, a JC-1 mitochondrial membrane potential detection kit (Beyotime Biotechnology, China) was used. .. HL-1 cells were seeded on sterile coverslips, cultured to the logarithmic phase, washed twice with prewarmed 1× PBS (Solarbio, Cat. No. P1010), and incubated with 1 mL of freshly prepared JC-1 staining working solution at 37 °C and 5% CO 2 in the dark for 20 min. After staining, the cells were washed twice with 1× JC-1 staining buffer, incubated with DAPI solution (G1012-100ML; Servicebio, China) at room temperature in the dark for 10 min for nuclear staining, mounted with anti-fluorescence quenching medium, and observed under a fluorescence microscope (red fluorescence for normal mitochondrial membrane potential, green fluorescence for decreased potential). ..

Inverted Microscopy:

Article Title: Exercise Alleviates Osteoporosis and Hyperglycemia in Type 1 Diabetes Mellitus Mice via Piezo1-Mediated Mechanotransduction.
Article Snippet: .. Nuclei were subsequently stained with DAPI solution (Servicebio, Wuhan, China) for 15 min. Fluorescence images were captured using an inverted microscope (Olympus IX73, Tokyo, Japan). ..

Sterility:

Article Title: Therapeutic potential of ADAR1-regulated macrophage exosomes for improving myocardial damage in septic cardiomyopathy
Article Snippet: To determine the mitochondrial membrane potential, a JC-1 mitochondrial membrane potential detection kit (Beyotime Biotechnology, China) was used. .. HL-1 cells were seeded on sterile coverslips, cultured to the logarithmic phase, washed twice with prewarmed 1× PBS (Solarbio, Cat. No. P1010), and incubated with 1 mL of freshly prepared JC-1 staining working solution at 37 °C and 5% CO 2 in the dark for 20 min. After staining, the cells were washed twice with 1× JC-1 staining buffer, incubated with DAPI solution (G1012-100ML; Servicebio, China) at room temperature in the dark for 10 min for nuclear staining, mounted with anti-fluorescence quenching medium, and observed under a fluorescence microscope (red fluorescence for normal mitochondrial membrane potential, green fluorescence for decreased potential). ..

Cell Culture:

Article Title: Therapeutic potential of ADAR1-regulated macrophage exosomes for improving myocardial damage in septic cardiomyopathy
Article Snippet: To determine the mitochondrial membrane potential, a JC-1 mitochondrial membrane potential detection kit (Beyotime Biotechnology, China) was used. .. HL-1 cells were seeded on sterile coverslips, cultured to the logarithmic phase, washed twice with prewarmed 1× PBS (Solarbio, Cat. No. P1010), and incubated with 1 mL of freshly prepared JC-1 staining working solution at 37 °C and 5% CO 2 in the dark for 20 min. After staining, the cells were washed twice with 1× JC-1 staining buffer, incubated with DAPI solution (G1012-100ML; Servicebio, China) at room temperature in the dark for 10 min for nuclear staining, mounted with anti-fluorescence quenching medium, and observed under a fluorescence microscope (red fluorescence for normal mitochondrial membrane potential, green fluorescence for decreased potential). ..

Membrane:

Article Title: Therapeutic potential of ADAR1-regulated macrophage exosomes for improving myocardial damage in septic cardiomyopathy
Article Snippet: To determine the mitochondrial membrane potential, a JC-1 mitochondrial membrane potential detection kit (Beyotime Biotechnology, China) was used. .. HL-1 cells were seeded on sterile coverslips, cultured to the logarithmic phase, washed twice with prewarmed 1× PBS (Solarbio, Cat. No. P1010), and incubated with 1 mL of freshly prepared JC-1 staining working solution at 37 °C and 5% CO 2 in the dark for 20 min. After staining, the cells were washed twice with 1× JC-1 staining buffer, incubated with DAPI solution (G1012-100ML; Servicebio, China) at room temperature in the dark for 10 min for nuclear staining, mounted with anti-fluorescence quenching medium, and observed under a fluorescence microscope (red fluorescence for normal mitochondrial membrane potential, green fluorescence for decreased potential). ..



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