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Dade Behring diffquick dade behring staining
Prevention of HDM-induced eosinophilia by Bacillus subtilis.(A) Timeline for B. subtilis and HDM treatment of 6 week-old C57Bl/6 mice. (B-E) Quantification of cells from bronchial alveolar lavage (BAL) cytospun and stained with <t>DiffQuick</t> on Day 12. Data represent mean ± SEM (n = 7–14) from 3 combined experiments. **** p < 0.0001, *** p < 0.0005 ** p < 0.005, * p < 0.05 by Student t test. o.g. = oral gavage; i.n. = intranasal.
Diffquick Dade Behring Staining, supplied by Dade Behring, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Article Title: Bacillus subtilis exopolysaccharide prevents allergic eosinophilia.

Journal: Allergy

doi: 10.1111/all.13674

Prevention of HDM-induced eosinophilia by Bacillus subtilis.(A) Timeline for B. subtilis and HDM treatment of 6 week-old C57Bl/6 mice. (B-E) Quantification of cells from bronchial alveolar lavage (BAL) cytospun and stained with DiffQuick on Day 12. Data represent mean ± SEM (n = 7–14) from 3 combined experiments. **** p < 0.0001, *** p < 0.0005 ** p < 0.005, * p < 0.05 by Student t test. o.g. = oral gavage; i.n. = intranasal.
Figure Legend Snippet: Prevention of HDM-induced eosinophilia by Bacillus subtilis.(A) Timeline for B. subtilis and HDM treatment of 6 week-old C57Bl/6 mice. (B-E) Quantification of cells from bronchial alveolar lavage (BAL) cytospun and stained with DiffQuick on Day 12. Data represent mean ± SEM (n = 7–14) from 3 combined experiments. **** p < 0.0001, *** p < 0.0005 ** p < 0.005, * p < 0.05 by Student t test. o.g. = oral gavage; i.n. = intranasal.

Techniques Used: Staining

Prevention of eosinophilia by EPS-treated dendritic cells.(A) Timeline for BMDC incubation with exopolysaccharide and HDM prior to i.n. transfer into 7–10 week-old C57Bl/6 mice. BMDC were generated from C57Bl/6 mouse bone marrow in the presence of 20ng/mL granulocyte-macrophage colony-stimulating factor (GM-CSF) for 6 days to >85% CD11c+ purity. Dendritic cell transfer groups included 1) No Treatment (No Txt) with BMDC receiving neither EPS nor HDM 2) HDM group with BMDC receiving HDM alone on D-2 and 3) EPS + HDM group of BMDC receiving EPS on D-2, followed by HDM on D-1. (B-E) Quantification of cells from BAL cytospun and stained with DiffQuick on Day 14. Data represent mean ± SEM (n = 3). *** p < 0.0005, ** p < 0.005, * p < 0.05 by Student t test. i.n.= intranasal. These data are representative of 4 independent BMDC transfer experiments.
Figure Legend Snippet: Prevention of eosinophilia by EPS-treated dendritic cells.(A) Timeline for BMDC incubation with exopolysaccharide and HDM prior to i.n. transfer into 7–10 week-old C57Bl/6 mice. BMDC were generated from C57Bl/6 mouse bone marrow in the presence of 20ng/mL granulocyte-macrophage colony-stimulating factor (GM-CSF) for 6 days to >85% CD11c+ purity. Dendritic cell transfer groups included 1) No Treatment (No Txt) with BMDC receiving neither EPS nor HDM 2) HDM group with BMDC receiving HDM alone on D-2 and 3) EPS + HDM group of BMDC receiving EPS on D-2, followed by HDM on D-1. (B-E) Quantification of cells from BAL cytospun and stained with DiffQuick on Day 14. Data represent mean ± SEM (n = 3). *** p < 0.0005, ** p < 0.005, * p < 0.05 by Student t test. i.n.= intranasal. These data are representative of 4 independent BMDC transfer experiments.

Techniques Used: Incubation, Generated, Staining

Related Articles

Cell Culture:

Article Title: Endothelial cell expression of galectin-1 induced by prostate cancer cells inhibits T-cell transendothelial migration.
Article Snippet: The following antibodies were used: biotinylated rabbit antihuman galectin-1 Ig prepared in our laboratory as previously described;11 goat anti-rabbit Ig-horseradish peroxidase (HRP), goat anti-rabbit Ig-FITC from Jackson ImmunoResearch Laboratories Inc. (West Grove, PA, USA); anti-CD43-FITC (clone S7) from BD Biosciences (Bedford, MA, USA). .. Reagents were purchased from the indicated suppliers: annexin V/propidium iodide (PI) from R&D Systems (Minneapolis, MN, USA); DTT from Fisher Scientific (Fairlawn, NJ, USA); PBS, ophenylenediamine dihydrochloride and BSA from Sigma (St Louis, MO, USA); Enhanced ChemiLuminescence (ECL) kit and Ficoll–Paque from Pharmacia LKB Biotechnology (Piscataway, NJ, USA); 5,6- carboxyfluorescein diacetate succinimidyl ester (CFSE) from Molecular Probes (Eugene, OR, USA); Matrigel and BioCoat Matrigel Invasion chamber (8 mm) from BD Biosciences; cell culture inserts (0.4 mm) for galectin-1 secretion assay from Falcon/ Becton Dickinson (Franklin Lakes, NJ, USA); DiffQuick staining solution from Dade Behring (Newark, DE, USA); benzyl-a-N-acetylgalactosamine (benzyla-GalNac) from CalBiochem (La Jolla, CA, USA); Zeocin from Invitrogen (Carlsbad, CA, USA); 96-well enhanced protein-binding enzyme linked immunosorbent assay (ELISA) plates (Immulon 2) from Thermo Labsystem (Franklin, MA, USA). ..

Staining:

Article Title: Endothelial cell expression of galectin-1 induced by prostate cancer cells inhibits T-cell transendothelial migration.
Article Snippet: The following antibodies were used: biotinylated rabbit antihuman galectin-1 Ig prepared in our laboratory as previously described;11 goat anti-rabbit Ig-horseradish peroxidase (HRP), goat anti-rabbit Ig-FITC from Jackson ImmunoResearch Laboratories Inc. (West Grove, PA, USA); anti-CD43-FITC (clone S7) from BD Biosciences (Bedford, MA, USA). .. Reagents were purchased from the indicated suppliers: annexin V/propidium iodide (PI) from R&D Systems (Minneapolis, MN, USA); DTT from Fisher Scientific (Fairlawn, NJ, USA); PBS, ophenylenediamine dihydrochloride and BSA from Sigma (St Louis, MO, USA); Enhanced ChemiLuminescence (ECL) kit and Ficoll–Paque from Pharmacia LKB Biotechnology (Piscataway, NJ, USA); 5,6- carboxyfluorescein diacetate succinimidyl ester (CFSE) from Molecular Probes (Eugene, OR, USA); Matrigel and BioCoat Matrigel Invasion chamber (8 mm) from BD Biosciences; cell culture inserts (0.4 mm) for galectin-1 secretion assay from Falcon/ Becton Dickinson (Franklin Lakes, NJ, USA); DiffQuick staining solution from Dade Behring (Newark, DE, USA); benzyl-a-N-acetylgalactosamine (benzyla-GalNac) from CalBiochem (La Jolla, CA, USA); Zeocin from Invitrogen (Carlsbad, CA, USA); 96-well enhanced protein-binding enzyme linked immunosorbent assay (ELISA) plates (Immulon 2) from Thermo Labsystem (Franklin, MA, USA). ..

Article Title: G-protein expression and intimal hyperplasia after arterial injury: a role for Galpha(i) proteins.
Article Snippet: .. The top portion of the filter was mechanically scraped, and smooth muscle cells that had migrated to the bottom side of the filter were fixed in a 100% solution of methanol, stained with DiffQuick staining solution (Dade International, Miami, Fla), and counted at 400× magnification to quantitate smooth muscle cell migration. ..

Article Title: Sphingosine 1-phosphate stimulates proliferation and migration of satellite cells: role of S1P receptors.
Article Snippet: CHEMOTX system, polycarbonate filters (8 μmpores) andmodified Boyden chambers were obtained from Neuroprobe (Gaithesburg, MD, USA). .. DiffQuick staining solution was purchased from Dade-Behring Holding GmbH (Liederbach, Germany). .. Male C57BL/6 mice, purchased from Charles River Laboratories Italia S.r.l. (Calco, Lecco, Italy), were housed at controlled temperature (21–24 °C) with a 12–12 h light–dark cycle.

Protein Binding:

Article Title: Endothelial cell expression of galectin-1 induced by prostate cancer cells inhibits T-cell transendothelial migration.
Article Snippet: The following antibodies were used: biotinylated rabbit antihuman galectin-1 Ig prepared in our laboratory as previously described;11 goat anti-rabbit Ig-horseradish peroxidase (HRP), goat anti-rabbit Ig-FITC from Jackson ImmunoResearch Laboratories Inc. (West Grove, PA, USA); anti-CD43-FITC (clone S7) from BD Biosciences (Bedford, MA, USA). .. Reagents were purchased from the indicated suppliers: annexin V/propidium iodide (PI) from R&D Systems (Minneapolis, MN, USA); DTT from Fisher Scientific (Fairlawn, NJ, USA); PBS, ophenylenediamine dihydrochloride and BSA from Sigma (St Louis, MO, USA); Enhanced ChemiLuminescence (ECL) kit and Ficoll–Paque from Pharmacia LKB Biotechnology (Piscataway, NJ, USA); 5,6- carboxyfluorescein diacetate succinimidyl ester (CFSE) from Molecular Probes (Eugene, OR, USA); Matrigel and BioCoat Matrigel Invasion chamber (8 mm) from BD Biosciences; cell culture inserts (0.4 mm) for galectin-1 secretion assay from Falcon/ Becton Dickinson (Franklin Lakes, NJ, USA); DiffQuick staining solution from Dade Behring (Newark, DE, USA); benzyl-a-N-acetylgalactosamine (benzyla-GalNac) from CalBiochem (La Jolla, CA, USA); Zeocin from Invitrogen (Carlsbad, CA, USA); 96-well enhanced protein-binding enzyme linked immunosorbent assay (ELISA) plates (Immulon 2) from Thermo Labsystem (Franklin, MA, USA). ..

Enzyme-linked Immunosorbent Assay:

Article Title: Endothelial cell expression of galectin-1 induced by prostate cancer cells inhibits T-cell transendothelial migration.
Article Snippet: The following antibodies were used: biotinylated rabbit antihuman galectin-1 Ig prepared in our laboratory as previously described;11 goat anti-rabbit Ig-horseradish peroxidase (HRP), goat anti-rabbit Ig-FITC from Jackson ImmunoResearch Laboratories Inc. (West Grove, PA, USA); anti-CD43-FITC (clone S7) from BD Biosciences (Bedford, MA, USA). .. Reagents were purchased from the indicated suppliers: annexin V/propidium iodide (PI) from R&D Systems (Minneapolis, MN, USA); DTT from Fisher Scientific (Fairlawn, NJ, USA); PBS, ophenylenediamine dihydrochloride and BSA from Sigma (St Louis, MO, USA); Enhanced ChemiLuminescence (ECL) kit and Ficoll–Paque from Pharmacia LKB Biotechnology (Piscataway, NJ, USA); 5,6- carboxyfluorescein diacetate succinimidyl ester (CFSE) from Molecular Probes (Eugene, OR, USA); Matrigel and BioCoat Matrigel Invasion chamber (8 mm) from BD Biosciences; cell culture inserts (0.4 mm) for galectin-1 secretion assay from Falcon/ Becton Dickinson (Franklin Lakes, NJ, USA); DiffQuick staining solution from Dade Behring (Newark, DE, USA); benzyl-a-N-acetylgalactosamine (benzyla-GalNac) from CalBiochem (La Jolla, CA, USA); Zeocin from Invitrogen (Carlsbad, CA, USA); 96-well enhanced protein-binding enzyme linked immunosorbent assay (ELISA) plates (Immulon 2) from Thermo Labsystem (Franklin, MA, USA). ..

Migration:

Article Title: G-protein expression and intimal hyperplasia after arterial injury: a role for Galpha(i) proteins.
Article Snippet: .. The top portion of the filter was mechanically scraped, and smooth muscle cells that had migrated to the bottom side of the filter were fixed in a 100% solution of methanol, stained with DiffQuick staining solution (Dade International, Miami, Fla), and counted at 400× magnification to quantitate smooth muscle cell migration. ..

other:

Article Title: Class IV Semaphorins Promote Angiogenesis by Stimulating Rho-Initiated Pathways through Plexin-B
Article Snippet: The semaphorins are a large family of secreted and cell surface proteins that provide attractive and repulsive cues for axon guidance during neuronal development.. Semaphorins share a conserved NH2-terminal Sema domain with their receptors, the plexins, which mediate neuronal cell adhesion, axon guidance, and maintenance of established neuronal pathways in the adult.. Both semaphorins and plexins share structural homology with the extracellular domain of c-Met, a member of the scatter factor family of receptors.

Article Title: Bacillus subtilis exopolysaccharide prevents allergic eosinophilia.
Article Snippet: Following the final HDM treatment, bronchial alveolar lavage (BAL) was collected from euthanized mice, by flushing the lungs with 0.8mL PBS containing 10% FCS, 1mM EDTA) and immune cell infiltration was assessed by DiffQuick (Dade Behring) staining of cytospun cells ( ).

Article Title: Malarial Hemozoin Activates the NLRP3 Inflammasome through Lyn and Syk Kinases
Article Snippet: Cells recovered from the peritoneum were counted and the percentage of neutrophils was determined from an H&E stain (DiffQuick; Dade Behring, Inc.) of a cytospun sample.



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Prevention of HDM-induced eosinophilia by Bacillus subtilis.(A) Timeline for B. subtilis and HDM treatment of 6 week-old C57Bl/6 mice. (B-E) Quantification of cells from bronchial alveolar lavage (BAL) cytospun and stained with <t>DiffQuick</t> on Day 12. Data represent mean ± SEM (n = 7–14) from 3 combined experiments. **** p < 0.0001, *** p < 0.0005 ** p < 0.005, * p < 0.05 by Student t test. o.g. = oral gavage; i.n. = intranasal.
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Prevention of HDM-induced eosinophilia by Bacillus subtilis.(A) Timeline for B. subtilis and HDM treatment of 6 week-old C57Bl/6 mice. (B-E) Quantification of cells from bronchial alveolar lavage (BAL) cytospun and stained with <t>DiffQuick</t> on Day 12. Data represent mean ± SEM (n = 7–14) from 3 combined experiments. **** p < 0.0001, *** p < 0.0005 ** p < 0.005, * p < 0.05 by Student t test. o.g. = oral gavage; i.n. = intranasal.
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Prevention of HDM-induced eosinophilia by Bacillus subtilis.(A) Timeline for B. subtilis and HDM treatment of 6 week-old C57Bl/6 mice. (B-E) Quantification of cells from bronchial alveolar lavage (BAL) cytospun and stained with <t>DiffQuick</t> on Day 12. Data represent mean ± SEM (n = 7–14) from 3 combined experiments. **** p < 0.0001, *** p < 0.0005 ** p < 0.005, * p < 0.05 by Student t test. o.g. = oral gavage; i.n. = intranasal.
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Image Search Results


Prevention of HDM-induced eosinophilia by Bacillus subtilis.(A) Timeline for B. subtilis and HDM treatment of 6 week-old C57Bl/6 mice. (B-E) Quantification of cells from bronchial alveolar lavage (BAL) cytospun and stained with DiffQuick on Day 12. Data represent mean ± SEM (n = 7–14) from 3 combined experiments. **** p < 0.0001, *** p < 0.0005 ** p < 0.005, * p < 0.05 by Student t test. o.g. = oral gavage; i.n. = intranasal.

Journal: Allergy

Article Title: Bacillus subtilis exopolysaccharide prevents allergic eosinophilia.

doi: 10.1111/all.13674

Figure Lengend Snippet: Prevention of HDM-induced eosinophilia by Bacillus subtilis.(A) Timeline for B. subtilis and HDM treatment of 6 week-old C57Bl/6 mice. (B-E) Quantification of cells from bronchial alveolar lavage (BAL) cytospun and stained with DiffQuick on Day 12. Data represent mean ± SEM (n = 7–14) from 3 combined experiments. **** p < 0.0001, *** p < 0.0005 ** p < 0.005, * p < 0.05 by Student t test. o.g. = oral gavage; i.n. = intranasal.

Article Snippet: Following the final HDM treatment, bronchial alveolar lavage (BAL) was collected from euthanized mice, by flushing the lungs with 0.8mL PBS containing 10% FCS, 1mM EDTA) and immune cell infiltration was assessed by DiffQuick (Dade Behring) staining of cytospun cells ( ).

Techniques: Staining

Prevention of eosinophilia by EPS-treated dendritic cells.(A) Timeline for BMDC incubation with exopolysaccharide and HDM prior to i.n. transfer into 7–10 week-old C57Bl/6 mice. BMDC were generated from C57Bl/6 mouse bone marrow in the presence of 20ng/mL granulocyte-macrophage colony-stimulating factor (GM-CSF) for 6 days to >85% CD11c+ purity. Dendritic cell transfer groups included 1) No Treatment (No Txt) with BMDC receiving neither EPS nor HDM 2) HDM group with BMDC receiving HDM alone on D-2 and 3) EPS + HDM group of BMDC receiving EPS on D-2, followed by HDM on D-1. (B-E) Quantification of cells from BAL cytospun and stained with DiffQuick on Day 14. Data represent mean ± SEM (n = 3). *** p < 0.0005, ** p < 0.005, * p < 0.05 by Student t test. i.n.= intranasal. These data are representative of 4 independent BMDC transfer experiments.

Journal: Allergy

Article Title: Bacillus subtilis exopolysaccharide prevents allergic eosinophilia.

doi: 10.1111/all.13674

Figure Lengend Snippet: Prevention of eosinophilia by EPS-treated dendritic cells.(A) Timeline for BMDC incubation with exopolysaccharide and HDM prior to i.n. transfer into 7–10 week-old C57Bl/6 mice. BMDC were generated from C57Bl/6 mouse bone marrow in the presence of 20ng/mL granulocyte-macrophage colony-stimulating factor (GM-CSF) for 6 days to >85% CD11c+ purity. Dendritic cell transfer groups included 1) No Treatment (No Txt) with BMDC receiving neither EPS nor HDM 2) HDM group with BMDC receiving HDM alone on D-2 and 3) EPS + HDM group of BMDC receiving EPS on D-2, followed by HDM on D-1. (B-E) Quantification of cells from BAL cytospun and stained with DiffQuick on Day 14. Data represent mean ± SEM (n = 3). *** p < 0.0005, ** p < 0.005, * p < 0.05 by Student t test. i.n.= intranasal. These data are representative of 4 independent BMDC transfer experiments.

Article Snippet: Following the final HDM treatment, bronchial alveolar lavage (BAL) was collected from euthanized mice, by flushing the lungs with 0.8mL PBS containing 10% FCS, 1mM EDTA) and immune cell infiltration was assessed by DiffQuick (Dade Behring) staining of cytospun cells ( ).

Techniques: Incubation, Generated, Staining