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    Tocris cppene
    Input to Cheriff-CA1 neurons 3 days after optogenetic STDP. (A) Dodt-contrast image (40x objective) of the CA1 region with overlaid epifluorescence image. Black asterisks: CheRiff-eGFP expressing CA1 pyramidal neurons; white asterisks: NT CA1 pyramidal neurons suitable for recording. Scale bar 25 μm. (B) Yellow light (1 ms, 594 nm) on ChrimsonR-CA3 neurons EPSCs in CA1 neurons of a control (no oSTDP pairing) slice. EPSCs are recorded sequentially from CheRiff-CA1 pyramidal neurons (green, average of 10 gray individual EPSCs) and at least 3 NT CA1 neurons (black average of 10 gray individual EPSCs). (C) Automatically detected EPSC peak (red x) and slope (dashed red line, 20–60% peak) from individual CheRiff-CA1 neurons and the average of NT neurons. (D–F) Red and violet ticks indicate pre- (red) <t>and</t> <t>post-synaptic</t> (violet) light stimulation. (D) Normalized input strength of CheRiff-CA1 neurons recorded from nonpaired control (left: no light stimulation; right: postsynaptic stimulation only) slices and mKate2-CA1 neurons 3 days later. mean ± SEM. n = 12; 7; 10 (left to right). (E) Normalized input strength of CheRiff-CA1 neurons 3 days after 300 pairings of single presynaptic and 3 postsynaptic spikes at 5 Hz. During anticausal pairing the last postsynaptic spike occurred −50 or − 10 ms before the EPSP. During causal pairing the first postsynaptic spike occurred +10 or + 50 ms after the EPSP. n = 10; 24; 25; 10 (left to right). * P < 0.05, *** P < 0.001. (F) Same as (E), but the pairing frequency was reduced to 0.1 Hz (360 pairings in 1 h) or the NMDA receptor antagonist <t>CPPene</t> (1 μM) was in the culture medium (±10 ms pairing 300x at 5 Hz. n = 5; 14; 14; 13, left to right). (G) Mean input strength (data from E) as a function of timing between EPSPs (red) and postsynaptic spike bursts (violet, at mean) at 5 Hz repetition frequency. Two complete cycles are illustrated.
    Cppene, supplied by Tocris, used in various techniques. Bioz Stars score: 92/100, based on 19 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/d-cpp-ene/D-CPP-ene/pmc09758582-40-16-19
    Average 92 stars, based on 19 article reviews
    cppene - by Bioz Stars, 2026-11
    92/100 stars

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    1) Product Images from "Spike-timing-dependent plasticity rewards synchrony rather than causality"

    Article Title: Spike-timing-dependent plasticity rewards synchrony rather than causality

    Journal: Cerebral Cortex (New York, NY)

    doi: 10.1093/cercor/bhac050

    Input to Cheriff-CA1 neurons 3 days after optogenetic STDP. (A) Dodt-contrast image (40x objective) of the CA1 region with overlaid epifluorescence image. Black asterisks: CheRiff-eGFP expressing CA1 pyramidal neurons; white asterisks: NT CA1 pyramidal neurons suitable for recording. Scale bar 25 μm. (B) Yellow light (1 ms, 594 nm) on ChrimsonR-CA3 neurons EPSCs in CA1 neurons of a control (no oSTDP pairing) slice. EPSCs are recorded sequentially from CheRiff-CA1 pyramidal neurons (green, average of 10 gray individual EPSCs) and at least 3 NT CA1 neurons (black average of 10 gray individual EPSCs). (C) Automatically detected EPSC peak (red x) and slope (dashed red line, 20–60% peak) from individual CheRiff-CA1 neurons and the average of NT neurons. (D–F) Red and violet ticks indicate pre- (red) and post-synaptic (violet) light stimulation. (D) Normalized input strength of CheRiff-CA1 neurons recorded from nonpaired control (left: no light stimulation; right: postsynaptic stimulation only) slices and mKate2-CA1 neurons 3 days later. mean ± SEM. n = 12; 7; 10 (left to right). (E) Normalized input strength of CheRiff-CA1 neurons 3 days after 300 pairings of single presynaptic and 3 postsynaptic spikes at 5 Hz. During anticausal pairing the last postsynaptic spike occurred −50 or − 10 ms before the EPSP. During causal pairing the first postsynaptic spike occurred +10 or + 50 ms after the EPSP. n = 10; 24; 25; 10 (left to right). * P < 0.05, *** P < 0.001. (F) Same as (E), but the pairing frequency was reduced to 0.1 Hz (360 pairings in 1 h) or the NMDA receptor antagonist CPPene (1 μM) was in the culture medium (±10 ms pairing 300x at 5 Hz. n = 5; 14; 14; 13, left to right). (G) Mean input strength (data from E) as a function of timing between EPSPs (red) and postsynaptic spike bursts (violet, at mean) at 5 Hz repetition frequency. Two complete cycles are illustrated.
    Figure Legend Snippet: Input to Cheriff-CA1 neurons 3 days after optogenetic STDP. (A) Dodt-contrast image (40x objective) of the CA1 region with overlaid epifluorescence image. Black asterisks: CheRiff-eGFP expressing CA1 pyramidal neurons; white asterisks: NT CA1 pyramidal neurons suitable for recording. Scale bar 25 μm. (B) Yellow light (1 ms, 594 nm) on ChrimsonR-CA3 neurons EPSCs in CA1 neurons of a control (no oSTDP pairing) slice. EPSCs are recorded sequentially from CheRiff-CA1 pyramidal neurons (green, average of 10 gray individual EPSCs) and at least 3 NT CA1 neurons (black average of 10 gray individual EPSCs). (C) Automatically detected EPSC peak (red x) and slope (dashed red line, 20–60% peak) from individual CheRiff-CA1 neurons and the average of NT neurons. (D–F) Red and violet ticks indicate pre- (red) and post-synaptic (violet) light stimulation. (D) Normalized input strength of CheRiff-CA1 neurons recorded from nonpaired control (left: no light stimulation; right: postsynaptic stimulation only) slices and mKate2-CA1 neurons 3 days later. mean ± SEM. n = 12; 7; 10 (left to right). (E) Normalized input strength of CheRiff-CA1 neurons 3 days after 300 pairings of single presynaptic and 3 postsynaptic spikes at 5 Hz. During anticausal pairing the last postsynaptic spike occurred −50 or − 10 ms before the EPSP. During causal pairing the first postsynaptic spike occurred +10 or + 50 ms after the EPSP. n = 10; 24; 25; 10 (left to right). * P < 0.05, *** P < 0.001. (F) Same as (E), but the pairing frequency was reduced to 0.1 Hz (360 pairings in 1 h) or the NMDA receptor antagonist CPPene (1 μM) was in the culture medium (±10 ms pairing 300x at 5 Hz. n = 5; 14; 14; 13, left to right). (G) Mean input strength (data from E) as a function of timing between EPSPs (red) and postsynaptic spike bursts (violet, at mean) at 5 Hz repetition frequency. Two complete cycles are illustrated.

    Techniques Used: Expressing, Control

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    Injection:

    Article Title: Transcranial Low-Intensity Focused Ultrasound Stimulation of the Visual Thalamus Produces Long-Term Depression of Thalamocortical Synapses in the Adult Visual Cortex
    Article Snippet: .. For a group of mice, NMDAR antagonist D-4-[(2E)-3-phosphono-2-propenyl]2-piperazinecarboxylic acid (D-CPP; Tocris Biosciences catalog #1265) was injected intraperitoneally (i.p., 10 mg/kg) 10–20 min prior to the LIFU stimulation to test the role of NMDARs. ..

    Transmission Assay:

    Article Title: Spike-timing-dependent plasticity rewards synchrony rather than causality
    Article Snippet: .. Whole cell currents were recorded (see electrophysiology below) with synaptic transmission and action potentials blocked by CPPene (10 μM, Tocris bioscience; 1265), NBQX (10 μM, Tocris bioscience; 1044), picrotoxin (100 μM, Sigma; P1675-1G) and tetrodotoxin (1 μM, HelloBio; HB1035). ..

    Article Title: Spike-timing-dependent plasticity rewards synchrony rather than causality
    Article Snippet: .. Whole cell currents were recorded (see electrophysiology below) with synaptic transmission and action potentials blocked by CPPene (10 μM, Tocris bioscience; 1265), NBQX (10 μM, Tocris bioscience; 1044), picrotoxin (100 μM, Sigma; P1675-1G), and tetrodotoxin (TTX, 1 μM, HelloBio; HB1035). ..

    Electrophysiology:

    Article Title: Spike-timing-dependent plasticity rewards synchrony rather than causality
    Article Snippet: .. Whole cell currents were recorded (see electrophysiology below) with synaptic transmission and action potentials blocked by CPPene (10 μM, Tocris bioscience; 1265), NBQX (10 μM, Tocris bioscience; 1044), picrotoxin (100 μM, Sigma; P1675-1G), and tetrodotoxin (TTX, 1 μM, HelloBio; HB1035). ..

    Blocking Assay:

    Article Title: PACmn for improved optogenetic control of intracellular cAMP
    Article Snippet: .. Recording temperature was 29 ± 1°C, and a mix of picrotoxin (100 μM), NBQX (10 μM), and CPPene (10 μM) (Tocris) was added to the extracellular solution to block synaptic responses. .. Where indicated, forskolin (FSK; 100 μM) and 3-isobutyl-1-methylxanthine (IBMX; 75 μM) (HelloBio) were added from concentrated stock solutions or an equivalent amount of dimethylsulfoxide (DMSO).

    Virus:

    Article Title: Optimized Design and in Vivo Application of Optogenetically Modified Drosophila Dopamine Receptors to Elucidate Endogenous Functions
    Article Snippet: .. ©@ CelPress STAR* METHODS KEY RESOURCES TABLE Neuron REAGENT or RESOURCE SOURCE IDENTIFIER Bacterial and virus strains rAAV2/18&2.CamKlla(0.4).OPN3-mScarlet This paper N/A rAAV2/1&2.CamKlla..eYFP.WPRE This paper N/A rAAV2/1&2.CamKla(0.4).eOPN3-mScarlet This paper www.addgene.org/125712/ rAAV2/1&2.hSyn.SIO-eOPN3-mScarlet This paper www.addgene.org/125713/ Chemicals, peptides, and recombinant proteins (R)-baclofen Tocris Cat#0796 Clozapine-N-Oxide Enzo Life Science Cat#-BML-NS105 CPPene Tocris Cat#1265 Gabazine Tocris Cat#1262 NBQX Tocris Cat#1044 Pertussis toxin Sigma-Aldrich Cat#516560 Picrotoxin Tocris Cat#1128 SCH23390 Tocris Cat#0925 Critical commercial assays GloSensor cAMP Assay Promega Cat#E1171 Experimental models: Cell lines HEK293T Sigma-Aldrich Cat#12022001 RRID:CVCL_0063 Experimental models: Organisms/strains Mouse: C57BL/6JRecHsd Envigo Cat#043 Mouse: C57BL/6NHsd Envigo Cat#044 Mouse: DAT-IRES-Cre The Jackson Laboratory Strain #006660 Rattus norvegicus: Sprague-Dawley Envigo Cat#002 Rattus norvegicus: Wistar Charles River, bred in the Cat#003 animal facility, UKE Hamburg Recombinant DNA pAAV-CaMkKlla(0.4)-OPN3-mScarlet This Paper N/A pAAV-CaMkKlla(0.4)-PufTMT3a-mScarlet This Paper N/A pAAV-CaMKlla(0.4)-OPN3-M4-mScarlet This Paper N/A pAAV-CaMKlla(0.4)-PufTMT3a-M4-mScarlet This Paper N/A pAAV-CamKlla-eYFP. pcDNA3.1-GIRK2-1 pcDNA3.1-mCerulean pAAV-CaMKlla(0.4)-eOPN3-mScarlet pAAV-hSyn-SIO-eOPN3-mScarlet Software and algorithms Fiji MATLAB 2018b Prism 8.2.1 RStudio Desktop Ephus. .. WaveSurfer e1 Neuron 109, 1621-1635.e1-e8, May 19, 2021 Karl Deisseroth Eitan Reuveny Dave Piston; Rizzo et al.,2004 This Paper This Paper Schindelin et al., 2012 Mathworks Graphpad RStudio Suter et al., 2010 Janelia 117 RRID:Addgene_105622; www.addgene.org/105622 GenBank: NM_001025584.2 RRID:Addgene_15214; www.addgene.org/15214/ RRID:Addgene_125712; www.addgene.org/125712/ RRID:Addgene_125713; www.addgene.org/125713/ RRID:SCR_0022885; http://imagej.net/Fiji RRID:SCR_001622; www.mathworks.com RRID:SCR_002798; https://www.graphpad.com RRID:SCR_000432; https://www.rstudio.com https://doi.org/10.3389/fncir.2010.00100 https://wavesurfer.janelia.org (Continued on next page)

    Recombinant:

    Article Title: Optimized Design and in Vivo Application of Optogenetically Modified Drosophila Dopamine Receptors to Elucidate Endogenous Functions
    Article Snippet: .. ©@ CelPress STAR* METHODS KEY RESOURCES TABLE Neuron REAGENT or RESOURCE SOURCE IDENTIFIER Bacterial and virus strains rAAV2/18&2.CamKlla(0.4).OPN3-mScarlet This paper N/A rAAV2/1&2.CamKlla..eYFP.WPRE This paper N/A rAAV2/1&2.CamKla(0.4).eOPN3-mScarlet This paper www.addgene.org/125712/ rAAV2/1&2.hSyn.SIO-eOPN3-mScarlet This paper www.addgene.org/125713/ Chemicals, peptides, and recombinant proteins (R)-baclofen Tocris Cat#0796 Clozapine-N-Oxide Enzo Life Science Cat#-BML-NS105 CPPene Tocris Cat#1265 Gabazine Tocris Cat#1262 NBQX Tocris Cat#1044 Pertussis toxin Sigma-Aldrich Cat#516560 Picrotoxin Tocris Cat#1128 SCH23390 Tocris Cat#0925 Critical commercial assays GloSensor cAMP Assay Promega Cat#E1171 Experimental models: Cell lines HEK293T Sigma-Aldrich Cat#12022001 RRID:CVCL_0063 Experimental models: Organisms/strains Mouse: C57BL/6JRecHsd Envigo Cat#043 Mouse: C57BL/6NHsd Envigo Cat#044 Mouse: DAT-IRES-Cre The Jackson Laboratory Strain #006660 Rattus norvegicus: Sprague-Dawley Envigo Cat#002 Rattus norvegicus: Wistar Charles River, bred in the Cat#003 animal facility, UKE Hamburg Recombinant DNA pAAV-CaMkKlla(0.4)-OPN3-mScarlet This Paper N/A pAAV-CaMkKlla(0.4)-PufTMT3a-mScarlet This Paper N/A pAAV-CaMKlla(0.4)-OPN3-M4-mScarlet This Paper N/A pAAV-CaMKlla(0.4)-PufTMT3a-M4-mScarlet This Paper N/A pAAV-CamKlla-eYFP. pcDNA3.1-GIRK2-1 pcDNA3.1-mCerulean pAAV-CaMKlla(0.4)-eOPN3-mScarlet pAAV-hSyn-SIO-eOPN3-mScarlet Software and algorithms Fiji MATLAB 2018b Prism 8.2.1 RStudio Desktop Ephus. .. WaveSurfer e1 Neuron 109, 1621-1635.e1-e8, May 19, 2021 Karl Deisseroth Eitan Reuveny Dave Piston; Rizzo et al.,2004 This Paper This Paper Schindelin et al., 2012 Mathworks Graphpad RStudio Suter et al., 2010 Janelia 117 RRID:Addgene_105622; www.addgene.org/105622 GenBank: NM_001025584.2 RRID:Addgene_15214; www.addgene.org/15214/ RRID:Addgene_125712; www.addgene.org/125712/ RRID:Addgene_125713; www.addgene.org/125713/ RRID:SCR_0022885; http://imagej.net/Fiji RRID:SCR_001622; www.mathworks.com RRID:SCR_002798; https://www.graphpad.com RRID:SCR_000432; https://www.rstudio.com https://doi.org/10.3389/fncir.2010.00100 https://wavesurfer.janelia.org (Continued on next page)

    cAMP Assay:

    Article Title: Optimized Design and in Vivo Application of Optogenetically Modified Drosophila Dopamine Receptors to Elucidate Endogenous Functions
    Article Snippet: .. ©@ CelPress STAR* METHODS KEY RESOURCES TABLE Neuron REAGENT or RESOURCE SOURCE IDENTIFIER Bacterial and virus strains rAAV2/18&2.CamKlla(0.4).OPN3-mScarlet This paper N/A rAAV2/1&2.CamKlla..eYFP.WPRE This paper N/A rAAV2/1&2.CamKla(0.4).eOPN3-mScarlet This paper www.addgene.org/125712/ rAAV2/1&2.hSyn.SIO-eOPN3-mScarlet This paper www.addgene.org/125713/ Chemicals, peptides, and recombinant proteins (R)-baclofen Tocris Cat#0796 Clozapine-N-Oxide Enzo Life Science Cat#-BML-NS105 CPPene Tocris Cat#1265 Gabazine Tocris Cat#1262 NBQX Tocris Cat#1044 Pertussis toxin Sigma-Aldrich Cat#516560 Picrotoxin Tocris Cat#1128 SCH23390 Tocris Cat#0925 Critical commercial assays GloSensor cAMP Assay Promega Cat#E1171 Experimental models: Cell lines HEK293T Sigma-Aldrich Cat#12022001 RRID:CVCL_0063 Experimental models: Organisms/strains Mouse: C57BL/6JRecHsd Envigo Cat#043 Mouse: C57BL/6NHsd Envigo Cat#044 Mouse: DAT-IRES-Cre The Jackson Laboratory Strain #006660 Rattus norvegicus: Sprague-Dawley Envigo Cat#002 Rattus norvegicus: Wistar Charles River, bred in the Cat#003 animal facility, UKE Hamburg Recombinant DNA pAAV-CaMkKlla(0.4)-OPN3-mScarlet This Paper N/A pAAV-CaMkKlla(0.4)-PufTMT3a-mScarlet This Paper N/A pAAV-CaMKlla(0.4)-OPN3-M4-mScarlet This Paper N/A pAAV-CaMKlla(0.4)-PufTMT3a-M4-mScarlet This Paper N/A pAAV-CamKlla-eYFP. pcDNA3.1-GIRK2-1 pcDNA3.1-mCerulean pAAV-CaMKlla(0.4)-eOPN3-mScarlet pAAV-hSyn-SIO-eOPN3-mScarlet Software and algorithms Fiji MATLAB 2018b Prism 8.2.1 RStudio Desktop Ephus. .. WaveSurfer e1 Neuron 109, 1621-1635.e1-e8, May 19, 2021 Karl Deisseroth Eitan Reuveny Dave Piston; Rizzo et al.,2004 This Paper This Paper Schindelin et al., 2012 Mathworks Graphpad RStudio Suter et al., 2010 Janelia 117 RRID:Addgene_105622; www.addgene.org/105622 GenBank: NM_001025584.2 RRID:Addgene_15214; www.addgene.org/15214/ RRID:Addgene_125712; www.addgene.org/125712/ RRID:Addgene_125713; www.addgene.org/125713/ RRID:SCR_0022885; http://imagej.net/Fiji RRID:SCR_001622; www.mathworks.com RRID:SCR_002798; https://www.graphpad.com RRID:SCR_000432; https://www.rstudio.com https://doi.org/10.3389/fncir.2010.00100 https://wavesurfer.janelia.org (Continued on next page)

    Software:

    Article Title: Optimized Design and in Vivo Application of Optogenetically Modified Drosophila Dopamine Receptors to Elucidate Endogenous Functions
    Article Snippet: .. ©@ CelPress STAR* METHODS KEY RESOURCES TABLE Neuron REAGENT or RESOURCE SOURCE IDENTIFIER Bacterial and virus strains rAAV2/18&2.CamKlla(0.4).OPN3-mScarlet This paper N/A rAAV2/1&2.CamKlla..eYFP.WPRE This paper N/A rAAV2/1&2.CamKla(0.4).eOPN3-mScarlet This paper www.addgene.org/125712/ rAAV2/1&2.hSyn.SIO-eOPN3-mScarlet This paper www.addgene.org/125713/ Chemicals, peptides, and recombinant proteins (R)-baclofen Tocris Cat#0796 Clozapine-N-Oxide Enzo Life Science Cat#-BML-NS105 CPPene Tocris Cat#1265 Gabazine Tocris Cat#1262 NBQX Tocris Cat#1044 Pertussis toxin Sigma-Aldrich Cat#516560 Picrotoxin Tocris Cat#1128 SCH23390 Tocris Cat#0925 Critical commercial assays GloSensor cAMP Assay Promega Cat#E1171 Experimental models: Cell lines HEK293T Sigma-Aldrich Cat#12022001 RRID:CVCL_0063 Experimental models: Organisms/strains Mouse: C57BL/6JRecHsd Envigo Cat#043 Mouse: C57BL/6NHsd Envigo Cat#044 Mouse: DAT-IRES-Cre The Jackson Laboratory Strain #006660 Rattus norvegicus: Sprague-Dawley Envigo Cat#002 Rattus norvegicus: Wistar Charles River, bred in the Cat#003 animal facility, UKE Hamburg Recombinant DNA pAAV-CaMkKlla(0.4)-OPN3-mScarlet This Paper N/A pAAV-CaMkKlla(0.4)-PufTMT3a-mScarlet This Paper N/A pAAV-CaMKlla(0.4)-OPN3-M4-mScarlet This Paper N/A pAAV-CaMKlla(0.4)-PufTMT3a-M4-mScarlet This Paper N/A pAAV-CamKlla-eYFP. pcDNA3.1-GIRK2-1 pcDNA3.1-mCerulean pAAV-CaMKlla(0.4)-eOPN3-mScarlet pAAV-hSyn-SIO-eOPN3-mScarlet Software and algorithms Fiji MATLAB 2018b Prism 8.2.1 RStudio Desktop Ephus. .. WaveSurfer e1 Neuron 109, 1621-1635.e1-e8, May 19, 2021 Karl Deisseroth Eitan Reuveny Dave Piston; Rizzo et al.,2004 This Paper This Paper Schindelin et al., 2012 Mathworks Graphpad RStudio Suter et al., 2010 Janelia 117 RRID:Addgene_105622; www.addgene.org/105622 GenBank: NM_001025584.2 RRID:Addgene_15214; www.addgene.org/15214/ RRID:Addgene_125712; www.addgene.org/125712/ RRID:Addgene_125713; www.addgene.org/125713/ RRID:SCR_0022885; http://imagej.net/Fiji RRID:SCR_001622; www.mathworks.com RRID:SCR_002798; https://www.graphpad.com RRID:SCR_000432; https://www.rstudio.com https://doi.org/10.3389/fncir.2010.00100 https://wavesurfer.janelia.org (Continued on next page)



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    Input to Cheriff-CA1 neurons 3 days after optogenetic STDP. (A) Dodt-contrast image (40x objective) of the CA1 region with overlaid epifluorescence image. Black asterisks: CheRiff-eGFP expressing CA1 pyramidal neurons; white asterisks: NT CA1 pyramidal neurons suitable for recording. Scale bar 25 μm. (B) Yellow light (1 ms, 594 nm) on ChrimsonR-CA3 neurons EPSCs in CA1 neurons of a control (no oSTDP pairing) slice. EPSCs are recorded sequentially from CheRiff-CA1 pyramidal neurons (green, average of 10 gray individual EPSCs) and at least 3 NT CA1 neurons (black average of 10 gray individual EPSCs). (C) Automatically detected EPSC peak (red x) and slope (dashed red line, 20–60% peak) from individual CheRiff-CA1 neurons and the average of NT neurons. (D–F) Red and violet ticks indicate pre- (red) <t>and</t> <t>post-synaptic</t> (violet) light stimulation. (D) Normalized input strength of CheRiff-CA1 neurons recorded from nonpaired control (left: no light stimulation; right: postsynaptic stimulation only) slices and mKate2-CA1 neurons 3 days later. mean ± SEM. n = 12; 7; 10 (left to right). (E) Normalized input strength of CheRiff-CA1 neurons 3 days after 300 pairings of single presynaptic and 3 postsynaptic spikes at 5 Hz. During anticausal pairing the last postsynaptic spike occurred −50 or − 10 ms before the EPSP. During causal pairing the first postsynaptic spike occurred +10 or + 50 ms after the EPSP. n = 10; 24; 25; 10 (left to right). * P < 0.05, *** P < 0.001. (F) Same as (E), but the pairing frequency was reduced to 0.1 Hz (360 pairings in 1 h) or the NMDA receptor antagonist <t>CPPene</t> (1 μM) was in the culture medium (±10 ms pairing 300x at 5 Hz. n = 5; 14; 14; 13, left to right). (G) Mean input strength (data from E) as a function of timing between EPSPs (red) and postsynaptic spike bursts (violet, at mean) at 5 Hz repetition frequency. Two complete cycles are illustrated.
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    Input to Cheriff-CA1 neurons 3 days after optogenetic STDP. (A) Dodt-contrast image (40x objective) of the CA1 region with overlaid epifluorescence image. Black asterisks: CheRiff-eGFP expressing CA1 pyramidal neurons; white asterisks: NT CA1 pyramidal neurons suitable for recording. Scale bar 25 μm. (B) Yellow light (1 ms, 594 nm) on ChrimsonR-CA3 neurons EPSCs in CA1 neurons of a control (no oSTDP pairing) slice. EPSCs are recorded sequentially from CheRiff-CA1 pyramidal neurons (green, average of 10 gray individual EPSCs) and at least 3 NT CA1 neurons (black average of 10 gray individual EPSCs). (C) Automatically detected EPSC peak (red x) and slope (dashed red line, 20–60% peak) from individual CheRiff-CA1 neurons and the average of NT neurons. (D–F) Red and violet ticks indicate pre- (red) <t>and</t> <t>post-synaptic</t> (violet) light stimulation. (D) Normalized input strength of CheRiff-CA1 neurons recorded from nonpaired control (left: no light stimulation; right: postsynaptic stimulation only) slices and mKate2-CA1 neurons 3 days later. mean ± SEM. n = 12; 7; 10 (left to right). (E) Normalized input strength of CheRiff-CA1 neurons 3 days after 300 pairings of single presynaptic and 3 postsynaptic spikes at 5 Hz. During anticausal pairing the last postsynaptic spike occurred −50 or − 10 ms before the EPSP. During causal pairing the first postsynaptic spike occurred +10 or + 50 ms after the EPSP. n = 10; 24; 25; 10 (left to right). * P < 0.05, *** P < 0.001. (F) Same as (E), but the pairing frequency was reduced to 0.1 Hz (360 pairings in 1 h) or the NMDA receptor antagonist <t>CPPene</t> (1 μM) was in the culture medium (±10 ms pairing 300x at 5 Hz. n = 5; 14; 14; 13, left to right). (G) Mean input strength (data from E) as a function of timing between EPSPs (red) and postsynaptic spike bursts (violet, at mean) at 5 Hz repetition frequency. Two complete cycles are illustrated.
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    Input to Cheriff-CA1 neurons 3 days after optogenetic STDP. (A) Dodt-contrast image (40x objective) of the CA1 region with overlaid epifluorescence image. Black asterisks: CheRiff-eGFP expressing CA1 pyramidal neurons; white asterisks: NT CA1 pyramidal neurons suitable for recording. Scale bar 25 μm. (B) Yellow light (1 ms, 594 nm) on ChrimsonR-CA3 neurons EPSCs in CA1 neurons of a control (no oSTDP pairing) slice. EPSCs are recorded sequentially from CheRiff-CA1 pyramidal neurons (green, average of 10 gray individual EPSCs) and at least 3 NT CA1 neurons (black average of 10 gray individual EPSCs). (C) Automatically detected EPSC peak (red x) and slope (dashed red line, 20–60% peak) from individual CheRiff-CA1 neurons and the average of NT neurons. (D–F) Red and violet ticks indicate pre- (red) and post-synaptic (violet) light stimulation. (D) Normalized input strength of CheRiff-CA1 neurons recorded from nonpaired control (left: no light stimulation; right: postsynaptic stimulation only) slices and mKate2-CA1 neurons 3 days later. mean ± SEM. n = 12; 7; 10 (left to right). (E) Normalized input strength of CheRiff-CA1 neurons 3 days after 300 pairings of single presynaptic and 3 postsynaptic spikes at 5 Hz. During anticausal pairing the last postsynaptic spike occurred −50 or − 10 ms before the EPSP. During causal pairing the first postsynaptic spike occurred +10 or + 50 ms after the EPSP. n = 10; 24; 25; 10 (left to right). * P < 0.05, *** P < 0.001. (F) Same as (E), but the pairing frequency was reduced to 0.1 Hz (360 pairings in 1 h) or the NMDA receptor antagonist CPPene (1 μM) was in the culture medium (±10 ms pairing 300x at 5 Hz. n = 5; 14; 14; 13, left to right). (G) Mean input strength (data from E) as a function of timing between EPSPs (red) and postsynaptic spike bursts (violet, at mean) at 5 Hz repetition frequency. Two complete cycles are illustrated.

    Journal: Cerebral Cortex (New York, NY)

    Article Title: Spike-timing-dependent plasticity rewards synchrony rather than causality

    doi: 10.1093/cercor/bhac050

    Figure Lengend Snippet: Input to Cheriff-CA1 neurons 3 days after optogenetic STDP. (A) Dodt-contrast image (40x objective) of the CA1 region with overlaid epifluorescence image. Black asterisks: CheRiff-eGFP expressing CA1 pyramidal neurons; white asterisks: NT CA1 pyramidal neurons suitable for recording. Scale bar 25 μm. (B) Yellow light (1 ms, 594 nm) on ChrimsonR-CA3 neurons EPSCs in CA1 neurons of a control (no oSTDP pairing) slice. EPSCs are recorded sequentially from CheRiff-CA1 pyramidal neurons (green, average of 10 gray individual EPSCs) and at least 3 NT CA1 neurons (black average of 10 gray individual EPSCs). (C) Automatically detected EPSC peak (red x) and slope (dashed red line, 20–60% peak) from individual CheRiff-CA1 neurons and the average of NT neurons. (D–F) Red and violet ticks indicate pre- (red) and post-synaptic (violet) light stimulation. (D) Normalized input strength of CheRiff-CA1 neurons recorded from nonpaired control (left: no light stimulation; right: postsynaptic stimulation only) slices and mKate2-CA1 neurons 3 days later. mean ± SEM. n = 12; 7; 10 (left to right). (E) Normalized input strength of CheRiff-CA1 neurons 3 days after 300 pairings of single presynaptic and 3 postsynaptic spikes at 5 Hz. During anticausal pairing the last postsynaptic spike occurred −50 or − 10 ms before the EPSP. During causal pairing the first postsynaptic spike occurred +10 or + 50 ms after the EPSP. n = 10; 24; 25; 10 (left to right). * P < 0.05, *** P < 0.001. (F) Same as (E), but the pairing frequency was reduced to 0.1 Hz (360 pairings in 1 h) or the NMDA receptor antagonist CPPene (1 μM) was in the culture medium (±10 ms pairing 300x at 5 Hz. n = 5; 14; 14; 13, left to right). (G) Mean input strength (data from E) as a function of timing between EPSPs (red) and postsynaptic spike bursts (violet, at mean) at 5 Hz repetition frequency. Two complete cycles are illustrated.

    Article Snippet: Whole cell currents were recorded (see electrophysiology below) with synaptic transmission and action potentials blocked by CPPene (10 μM, Tocris bioscience; 1265), NBQX (10 μM, Tocris bioscience; 1044), picrotoxin (100 μM, Sigma; P1675-1G), and tetrodotoxin (TTX, 1 μM, HelloBio; HB1035).

    Techniques: Expressing, Control