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customarraytm 12k arrays  (CombiMatrix)

 
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    CombiMatrix customarraytm 12k arrays
    Customarraytm 12k Arrays, supplied by CombiMatrix, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/customarraytm+12k+arrays/customarraytm/pm18843300-64-8-11
    Average 90 stars, based on 1 article reviews
    customarraytm 12k arrays - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    other:

    Article Title: Correction: High throughput approaches reveal splicing of primary microRNA transcripts and tissue specific expression of mature microRNAs in Vitis vinifera.
    Article Snippet: The sharp up-regulation of miR395 at veraison suggests a further role for miRNAs in an agronomically Figure 1 Oligonucleotide design strategy for Combimatrix custom oligonucleotide array.

    Microarray:

    Article Title: Response of the goat mammary gland to infection with Staphylococcus aureus revealed by gene expression profiling in milk somatic and white blood cells
    Article Snippet: The purified labelled aRNA was quantified using a NanoDrop spectrophotometer (NanoDrop Technologies, Wilmington, DE, USA). .. Four μg of labelled RNA were fragmented to a uniform size and hybridized to the custom array following the Combimatrix CustomArray 90K Microarray Hybridization and Imaging Protocol Arrays were stripped and re-hybridised using the CustomArray Stripping Kit for 90K (CombiMatrix Cat. No. 610049) following the protocols of the manufacturer. ..

    Hybridization:

    Article Title: Response of the goat mammary gland to infection with Staphylococcus aureus revealed by gene expression profiling in milk somatic and white blood cells
    Article Snippet: The purified labelled aRNA was quantified using a NanoDrop spectrophotometer (NanoDrop Technologies, Wilmington, DE, USA). .. Four μg of labelled RNA were fragmented to a uniform size and hybridized to the custom array following the Combimatrix CustomArray 90K Microarray Hybridization and Imaging Protocol Arrays were stripped and re-hybridised using the CustomArray Stripping Kit for 90K (CombiMatrix Cat. No. 610049) following the protocols of the manufacturer. ..

    Imaging:

    Article Title: Response of the goat mammary gland to infection with Staphylococcus aureus revealed by gene expression profiling in milk somatic and white blood cells
    Article Snippet: The purified labelled aRNA was quantified using a NanoDrop spectrophotometer (NanoDrop Technologies, Wilmington, DE, USA). .. Four μg of labelled RNA were fragmented to a uniform size and hybridized to the custom array following the Combimatrix CustomArray 90K Microarray Hybridization and Imaging Protocol Arrays were stripped and re-hybridised using the CustomArray Stripping Kit for 90K (CombiMatrix Cat. No. 610049) following the protocols of the manufacturer. ..

    Stripping Membranes:

    Article Title: Response of the goat mammary gland to infection with Staphylococcus aureus revealed by gene expression profiling in milk somatic and white blood cells
    Article Snippet: The purified labelled aRNA was quantified using a NanoDrop spectrophotometer (NanoDrop Technologies, Wilmington, DE, USA). .. Four μg of labelled RNA were fragmented to a uniform size and hybridized to the custom array following the Combimatrix CustomArray 90K Microarray Hybridization and Imaging Protocol Arrays were stripped and re-hybridised using the CustomArray Stripping Kit for 90K (CombiMatrix Cat. No. 610049) following the protocols of the manufacturer. ..

    Synthesized:

    Article Title: Electrochemically Generated Acid and Its Containment to 100 Micron Reaction Areas for the Production of DNA Microarrays
    Article Snippet: .. A CombiMatrix custom array chip was synthesized using a pattern, which provided 4 to 8 separate areas of the chip to be used in a sequential manner. ..

    DNA Array:

    Article Title: Electrochemically Generated Acid and Its Containment to 100 Micron Reaction Areas for the Production of DNA Microarrays
    Article Snippet: .. In general, the production of a DNA array is as follows: A CombiMatrix custom array chip was placed in the chamber of a DNA synthesizer programmed to run standard cyanoethyl phosphoramidite DNA synthesis. ..

    DNA Synthesis:

    Article Title: Electrochemically Generated Acid and Its Containment to 100 Micron Reaction Areas for the Production of DNA Microarrays
    Article Snippet: .. In general, the production of a DNA array is as follows: A CombiMatrix custom array chip was placed in the chamber of a DNA synthesizer programmed to run standard cyanoethyl phosphoramidite DNA synthesis. ..

    Expressing:

    Article Title: Overexpression of genes of the fatty acid biosynthetic pathway leads to accumulation of sterols in Saccharomyces cerevisiae.
    Article Snippet: Saccharomyces cerevisiae strains with deregulated sterol and fatty acid biosynthesis pathways were analysed for sterol and fatty acid content and mRNA profiles, with the aim of identifying interactions between lipid biosynthesis pathways.. Acetyl CoA carboxylase ACC1 and fatty acid synthases FAS1/FAS2 were overexpressed in wildtype and squalene-overproducing strains.. ACC1 overexpression led to decreased fatty acid content in the squalene-overproducing strain (factor of 0.7), while sterols and squalene were increased (factor of 1.5).

    Gene Expression:

    Article Title: Overexpression of genes of the fatty acid biosynthetic pathway leads to accumulation of sterols in Saccharomyces cerevisiae.
    Article Snippet: Saccharomyces cerevisiae strains with deregulated sterol and fatty acid biosynthesis pathways were analysed for sterol and fatty acid content and mRNA profiles, with the aim of identifying interactions between lipid biosynthesis pathways.. Acetyl CoA carboxylase ACC1 and fatty acid synthases FAS1/FAS2 were overexpressed in wildtype and squalene-overproducing strains.. ACC1 overexpression led to decreased fatty acid content in the squalene-overproducing strain (factor of 0.7), while sterols and squalene were increased (factor of 1.5).

    Software:

    Article Title: Biosynthesis of Proanthocyanidins in White Clover Flowers: Cross Talk within the Flavonoid Pathway
    Article Snippet: .. Proprietary CombiMatrix (Mukilteo) Custom Array software was used to design single-oligonucleotide probes of 35 to 40 bases in length for each white clover unigene. .. The resulting probe set was then assigned to a custom-made 12K CombiMatrix oligonucleotide array.



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    customarraytm 12k arrays  (CombiMatrix)
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    CombiMatrix customarraytm 12k arrays
    Customarraytm 12k Arrays, supplied by CombiMatrix, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/customarraytm+12k+arrays/customarraytm/pmc02680965-120-4-6
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    custom microarray customarraytm 12k arrays  (CombiMatrix)
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    CombiMatrix custom microarray customarraytm 12k arrays
    Custom microarrays analysis . A) Bar plot of UCEs expressed across 4 mouse developmental stages tested (ES, E12.5, E14.5 and E16.5) based on the analysis of our UCE custom <t>microarray.</t> Blue bars indicate UCEs which show expression on a single strand, green columns indicate UCEs which show expression on both strands B) Venn diagram of UCE transcription results showing the overlap across the 4 stages analyzed. More than half (n = 140, 56%) of the transcribed UCEs are expressed in all the stages analyzed.
    Custom Microarray Customarraytm 12k Arrays, supplied by CombiMatrix, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/customarraytm+12k+arrays/4x2k+custom+array/pmc02847969-26-4-10
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    Image Search Results


    Custom microarrays analysis . A) Bar plot of UCEs expressed across 4 mouse developmental stages tested (ES, E12.5, E14.5 and E16.5) based on the analysis of our UCE custom microarray. Blue bars indicate UCEs which show expression on a single strand, green columns indicate UCEs which show expression on both strands B) Venn diagram of UCE transcription results showing the overlap across the 4 stages analyzed. More than half (n = 140, 56%) of the transcribed UCEs are expressed in all the stages analyzed.

    Journal: BMC Genomics

    Article Title: Promiscuity of enhancer, coding and non-coding transcription functions in ultraconserved elements

    doi: 10.1186/1471-2164-11-151

    Figure Lengend Snippet: Custom microarrays analysis . A) Bar plot of UCEs expressed across 4 mouse developmental stages tested (ES, E12.5, E14.5 and E16.5) based on the analysis of our UCE custom microarray. Blue bars indicate UCEs which show expression on a single strand, green columns indicate UCEs which show expression on both strands B) Venn diagram of UCE transcription results showing the overlap across the 4 stages analyzed. More than half (n = 140, 56%) of the transcribed UCEs are expressed in all the stages analyzed.

    Article Snippet: We therefore designed a custom microarray (CustomarrayTM 12K arrays from Combimatrix, Mukilteo, WA) encompassing 3 different probes on each DNA strand of UCEs (of the currently annotated 481 UCEs, probes could be designed for 475), as well as a large number of negative controls (exogenous sequences from bacteria and plants, negative controls used in the Affymetrix platform, rRNAs sequences), which were used to assess the levels of background signal.

    Techniques: Microarray, Expressing

    UCEs transcription and enhancer function overlap . Overlap between the enhancer dataset (Pennacchio et al, 2006) and the mouse microarray dataset in all samples analyzed, divided by stage. The yellow portion of each bar indicates UCEs that are only transcribed, the green portion UCEs that are transcribed and act as enhancers, the blue portion UCEs that are only transcribed.

    Journal: BMC Genomics

    Article Title: Promiscuity of enhancer, coding and non-coding transcription functions in ultraconserved elements

    doi: 10.1186/1471-2164-11-151

    Figure Lengend Snippet: UCEs transcription and enhancer function overlap . Overlap between the enhancer dataset (Pennacchio et al, 2006) and the mouse microarray dataset in all samples analyzed, divided by stage. The yellow portion of each bar indicates UCEs that are only transcribed, the green portion UCEs that are transcribed and act as enhancers, the blue portion UCEs that are only transcribed.

    Article Snippet: We therefore designed a custom microarray (CustomarrayTM 12K arrays from Combimatrix, Mukilteo, WA) encompassing 3 different probes on each DNA strand of UCEs (of the currently annotated 481 UCEs, probes could be designed for 475), as well as a large number of negative controls (exogenous sequences from bacteria and plants, negative controls used in the Affymetrix platform, rRNAs sequences), which were used to assess the levels of background signal.

    Techniques: Microarray

    UCE classification using External datasets . A) Comparison between the mouse enhancer dataset (Pennacchio et al. 2006) (green oval), our mouse development microarray dataset, (red oval), the human UCE expression dataset (Calin et al. 2007) (blue oval) and the mouse ES cell SOLiD expression dataset (Cloonan et al. 2008) (orange oval). B) Comparison of the SOLiD ES cell RNAseq dataset (Cloonan et al. 2008) for UCEs vs. randomly chosen non-transcribed genomic regions (outliers not shown).

    Journal: BMC Genomics

    Article Title: Promiscuity of enhancer, coding and non-coding transcription functions in ultraconserved elements

    doi: 10.1186/1471-2164-11-151

    Figure Lengend Snippet: UCE classification using External datasets . A) Comparison between the mouse enhancer dataset (Pennacchio et al. 2006) (green oval), our mouse development microarray dataset, (red oval), the human UCE expression dataset (Calin et al. 2007) (blue oval) and the mouse ES cell SOLiD expression dataset (Cloonan et al. 2008) (orange oval). B) Comparison of the SOLiD ES cell RNAseq dataset (Cloonan et al. 2008) for UCEs vs. randomly chosen non-transcribed genomic regions (outliers not shown).

    Article Snippet: We therefore designed a custom microarray (CustomarrayTM 12K arrays from Combimatrix, Mukilteo, WA) encompassing 3 different probes on each DNA strand of UCEs (of the currently annotated 481 UCEs, probes could be designed for 475), as well as a large number of negative controls (exogenous sequences from bacteria and plants, negative controls used in the Affymetrix platform, rRNAs sequences), which were used to assess the levels of background signal.

    Techniques: Comparison, Microarray, Expressing