custom arraystar mirna target prediction software (Arraystar inc)
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Custom Arraystar Mirna Target Prediction Software, supplied by Arraystar inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
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1) Product Images from "Circular RNA hsa_circ_0000326 acts as a miR-338-3p sponge to facilitate lung adenocarcinoma progression"
Article Title: Circular RNA hsa_circ_0000326 acts as a miR-338-3p sponge to facilitate lung adenocarcinoma progression
Journal: Journal of Experimental & Clinical Cancer Research : CR
doi: 10.1186/s13046-020-01556-4
Figure Legend Snippet: Hsa_circ_0000326 acted as a sponge of miR-338-3p. ( a ) Putative targets of hsa_circ_0000326 were predicted using custom Arraystar software. ( b ) Effects of five predicted miRNAs and a negative control on the luciferase activity of hsa_circ_0000326, as determined by luciferase reporter assay. ( c ) The results of the luciferase reporter assay validated the interaction between hsa_circ_0000326 and miR-338-3p. ( d ) Colocalization between hsa_circ_0000326 and miR-338-3p was observed (arrowheads) using RNA-FISH in A549 cells. The nuclei were stained with DAPI. Scale bar = 40 μm. ( e ) RNA pulldown assay in A549 cells showed hsa_circ_0000326 could interact with miR-338-3p. The bars with error bars represent the mean ± SD from three independent experiments. * P < 0.05
Techniques Used: Software, Negative Control, Luciferase, Activity Assay, Reporter Assay, Staining
Figure Legend Snippet: Schematic of the biological roles of hsa_circ_0000326 in lung adenocarcinoma carcinogenesis. Hsa_circ_0000326 could bind to miR-338-3p as a miRNA sponge, exerting its function via regulation of the downstream target RAB14. Knockdown of hsa_circ_0000326 could inhibit lung adenocarcinoma cell proliferation and migration and promote apoptosis in vitro and inhibit tumor formation in vivo
Techniques Used: Knockdown, Migration, In Vitro, In Vivo
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