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intelligent automation systems custom-built arrayer  (Brooks Automation)

 
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    Structured Review

    Brooks Automation intelligent automation systems custom-built arrayer
    Intelligent Automation Systems Custom Built Arrayer, supplied by Brooks Automation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/custom+arrayer/intelligent+automation+systems+custom+built+arrayer/pmc02556097-303-9-10
    Average 90 stars, based on 1 article reviews
    intelligent automation systems custom-built arrayer - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    other:

    Article Title: Refinement of Light-Responsive Transcript Lists Using Rice Oligonucleotide Arrays: Evaluation of Gene-Redundancy
    Article Snippet: Spotting was performed using an Intelligent Automation Systems custom-built arrayer (Brooks Automation, Chelmsford, MA) with a 48-pin print head.

    Article Title: Identification, Distribution, and Expression of Novel Genes in 10 Clinical Isolates of Nontypeable Haemophilus influenzae
    Article Snippet: Additional sequencing was performed using an ABI 3730 DNA analyzer in which 3-μl reactions were prepared, using a Parallab 350-nanoliter genomic workstation (Brooks Automation, Inc., Chelmsford, MA), consisting of (i) 1.4 μl of plasmid template (approximately 100 ng DNA), (ii) 0.5 μl of primer (10 pmol/μl), and (iii) 1.1 μl of a BigDye Terminator v.3.1 cycle sequencing kit (Applied Biosystems, Inc.) ( 63 ).

    Article Title: Refinement of light-responsive transcript lists using rice oligonucleotide arrays: evaluation of gene-redundancy.
    Article Snippet: Spotting was performed using an Intelligent Automation Systems custom-built arrayer (Brooks Automation, Chelmsford, MA) with a 48-pin print head.

    Article Title: Identification, Distribution, and Expression of Novel Genes in 10 Clinical Isolates of Nontypeable Haemophilus influenzae
    Article Snippet: Additional sequencing was performed using an ABI 3730 DNA analyzer in which 3- l reactions were prepared, using a Parallab 350-nanoliter genomic workstation (Brooks Automation, Inc., Chelmsford, MA), consisting of (i) 1.4 l of plasmid template (approximately 100 ng DNA), (ii) 0.5 l of primer (10 pmol/ l), and (iii) 1.1 l of a BigDye Terminator v.3.1 cycle sequencing kit (Applied Biosystems, Inc.) (63).

    Sequencing:

    Article Title: Extensive Genomic Plasticity in Pseudomonas aeruginosa Revealed by Identification and Distribution Studies of Novel Genes among Clinical Isolates
    Article Snippet: Dideoxy sequencing was performed as described for both LiCor IR 2 Gene ReadIR instruments (LiCor, Inc., Lincoln, Nebr.) and Beckman CEQ 2000 XL automated capillary electrophoresis sequencing instruments (Beckman Coulter, Inc., Fullerton, CA) ( 44 ). .. Confirmatory sequencing was performed using an ABI 3730xl DNA analyzer (Applied Biosystems Inc., Foster City, CA) in which the sequencing reactions were prepared in 3-μl volumes, using the Parallab 350 nanoliter genomic work station (Brooks Automation, Inc., Chelmsford, MA). .. For Parallab-based sequencing, the reaction mixtures consisted of the following: (i) 1.4 μl of plasmid template (approximately 100 ng DNA); (ii) 0.5 μl of 10-pmol/μl primer; and (iii) 1.1 μl from the BigDye Terminator v3.1 cycle sequencing kit (Applied Biosystems).

    Purification:

    Article Title: Characterization, Distribution, and Expression of Novel Genes among Eight Clinical Isolates of Streptococcus pneumoniae
    Article Snippet: Sequencing reaction mixtures were prepared in a 3-μl volume consisting of the following reagents: 1.4 μl of plasmid template (approximately 100 ng DNA), 0.5 μl of 10-pmol/μl primer, and 1.1 μl of BigDye Terminator v3.1 cycle sequencing kit mix (Applied Biosystems Inc., Foster City, CA). .. Reaction aliquots of 0.5 μl were then subjected to thermal cycling and purified using the nano-pipetter of a Parallab 350 nanoliter genomic workstation (Brooks Automation, Inc., Chelmsford, MA). ..

    Article Title: Characterization, Distribution, and Expression of Novel Genes among Eight Clinical Isolates of Streptococcuspneumoniae
    Article Snippet: Sequencing reaction mixtures were prepared in a 3- l volume consisting of the following reagents: 1.4 l of plasmid template (approximately 100 ng DNA), 0.5 l of 10-pmol/ l primer, and 1.1 l of BigDye Terminator v3.1 cycle sequencing kit mix (Applied Biosystems Inc., Foster City, CA). .. Reaction aliquots of 0.5 l were then subjected to thermal cycling and purified using the nano-pipetter of a Parallab 350 nanoliter genomic workstation (Brooks Automation, Inc., Chelmsford, MA). ..

    Amplification:

    Article Title: Amplification and cloning of single DNA molecules using rolling circle amplification
    Article Snippet: The method comprises combining in a reaction chamber, such as a glass capillary tube, preferably in a volume of about 200 nl or less (e.g., a volume of about 64 nl or less), a reaction mixture comprising the DNA template, a DNA polymerase, and a mixture of suitable primers; and incubating the reaction mixture under conditions that are effective for promoting DNA strand displacement, at a substantially isothermal temperature. .. In one embodiment of this method, the amplification reactions are carried out in an automated nanoliter system, such as the Parallab 350 machine manufactured by Brooks Automation Inc., 15 Elizabeth Drive, Chelmsford, Mass., 01824, USA. ..



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