sigmaplot curve-fitting program (Jandel Engineering)
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Jandel Engineering
sigmaplot curve-fitting program
Sigmaplot Curve Fitting Program, supplied by Jandel Engineering, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/curve-fit+program+sigmaplot/curve+fitting+program+sigmaplot/pm24766192-93-6-9
Average 90 stars, based on 1 article reviews
Sigmaplot Curve Fitting Program, supplied by Jandel Engineering, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/curve-fit+program+sigmaplot/curve+fitting+program+sigmaplot/pm24766192-93-6-9
Average 90 stars, based on 1 article reviews
sigmaplot curve-fitting program - by Bioz Stars,
2026-10
90/100 stars
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Activity Assay:Article Title: Elicitor induced defence responses in medicago sativa. Article Snippet: .. Table 4 Superoxide synthesizing activity of the microsomal membrane fraction from 5-d-old cultures of Medicago Sativa cells, assayed with either NADH or NADPH as the source of reductant Superoxide synthesizing activitya (μmol min−1 mg−1 protein) NADH NADPH Total 0.46 0.82 +KCN (2 mM) 0.41 0.88 +NaN3 (10 mM) 0.39 0.82 IC50 b (μM) +DPI Component A 1.25 1.76 Component B 7.42 11.74 Contribution to total response A : Bb 2 : 3 2 : 3 aSuperoxide synthesizing activity was calculated as the difference between the oxidation of NADH or NADPH in the presence and absence of superoxide dismutase (SOD) (100 units ml−1). bDetermined from analyses of the data of Fig. 9 with a Membrane:Article Title: Elicitor induced defence responses in medicago sativa. Article Snippet: .. Table 4 Superoxide synthesizing activity of the microsomal membrane fraction from 5-d-old cultures of Medicago Sativa cells, assayed with either NADH or NADPH as the source of reductant Superoxide synthesizing activitya (μmol min−1 mg−1 protein) NADH NADPH Total 0.46 0.82 +KCN (2 mM) 0.41 0.88 +NaN3 (10 mM) 0.39 0.82 IC50 b (μM) +DPI Component A 1.25 1.76 Component B 7.42 11.74 Contribution to total response A : Bb 2 : 3 2 : 3 aSuperoxide synthesizing activity was calculated as the difference between the oxidation of NADH or NADPH in the presence and absence of superoxide dismutase (SOD) (100 units ml−1). bDetermined from analyses of the data of Fig. 9 with a Software:Article Title: Synthesis of classical, four-carbon bridged 5-substituted furo[2,3- d ]pyrimidine and 6-substituted pyrrolo[2,3- d ]pyrimidine analogues as antifolates Article Snippet: .. A 4th ml of 0.1 N HCl (which recovers the polyglutamate products) was required to obtain these recoveries of each analogue, however kinetic constants were determined by the hyperbolic Article Title: Dual Inhibitors of Thymidylate Synthase and Dihydrofolate Reductase as Antitumour Agents: Design, Synthesis and Biological Evaluation of Classical and Nonclassical Pyrrolo[2,3- d ]pyrimidine Antifolates Article Snippet: .. Kinetic constants for substrates were determined by the hyperbolic Article Title: Synthesis and evaluation of a classical 2,4-diamino-5-substituted-furo[2,3-d]pyrimidine and a 2-amino-4-oxo-6-substituted-pyrrolo[2,3-d]pyrimidine as antifolates Article Snippet: .. Kinetic constants were determined by the hyperbolic Concentration Assay:Article Title: Synthesis of classical, four-carbon bridged 5-substituted furo[2,3- d ]pyrimidine and 6-substituted pyrrolo[2,3- d ]pyrimidine analogues as antifolates Article Snippet: .. A 4th ml of 0.1 N HCl (which recovers the polyglutamate products) was required to obtain these recoveries of each analogue, however kinetic constants were determined by the hyperbolic Article Title: Dual Inhibitors of Thymidylate Synthase and Dihydrofolate Reductase as Antitumour Agents: Design, Synthesis and Biological Evaluation of Classical and Nonclassical Pyrrolo[2,3- d ]pyrimidine Antifolates Article Snippet: .. Kinetic constants for substrates were determined by the hyperbolic Article Title: Synthesis and evaluation of a classical 2,4-diamino-5-substituted-furo[2,3-d]pyrimidine and a 2-amino-4-oxo-6-substituted-pyrrolo[2,3-d]pyrimidine as antifolates Article Snippet: .. Kinetic constants were determined by the hyperbolic Article Title: Use of microproteins as tryptase inhibitors Article Snippet: For calculation of Kiapp-values, vmax values were determined and the curve fitting tool of SigmaPlot (Jandel Scientific) was applied on the basis of the following formula, that describes the case of competitive tight binding inhibitors (Morrison, 1969): v 1 v 0 = 1 - [ E t ] + [ I t ] + K iapp - { ( [ E t ] + [ I t ] + K iapp ) 2 - 4 [ E t ] · [ I t ] } 1 2 2 [ E t ] vi=vmax value of inhibited reaction v0=vmax value of uninhibited reaction [Et]=total enzyme concentration (free and in complex) [It]=total inhibitor concentration (free and in complex) Kiapp=apparent dissociation constant of the enzyme-inhibitor complex. .. For calculation of Kiapp-values, vmax values were determined and the other:Article Title: Daytime restricted feeding modifies the daily variations of liver gluconeogenesis: adaptations in biochemical and endocrine regulators. Article Snippet: Daytime restricted feeding (DRF) promotes circadian adaptations in the metabolic processing of nutrients.. We explored the hepatic gluconeogenic response in DRF rats by the temporal profiles of the following: (1) the activity of glucose 6-phosphatase (G6Pase) and phosphoenolpyruvate carboxykinase (PEPCK), as well as the periportal and pericentral distribution of PEPCK; (2) conversion of alanine to glucose; (3) glycemia and liver glycogen content; (4) presence of glycogen synthase (GYS) and its phosphorylated form (at Ser641, pGYS); (5) circulating levels of corticosterone, glucagon and insulin; (6) glucose-tolerance test; and (7) sirtuin 1 (SIRT1) and peroxisome proliferatoractivated receptor-coactivator 1a (PGC-1a).. The results showed that DRF promoted: (1) a phase shift in G6Pase activity and an increase in PEPCK activity as well as a change of PEPCK from periportal to pericentral hepatocytes, (2) a net conversion of alanine to circulating glucose, (3) a decrease in glycemic values and a phase shift in the liver glycogen content, (4) a phase shift in GYS and an increase of pGYS, (5) an increase in the daily levels of corticosterone and glucagon, but a reduction in the levels of insulin, (6) normal glucose homeostasis in all groups and (7) an enhanced presence of SIRT1 and PGC-1a. Article Title: Restricted feeding entrains rhythms of inflammation-related factors without promoting an acute-phase response. Article Snippet: A restricted schedule of food access promotes numerous metabolic and physiological adaptations to optimize the biochemical handling of nutrients.. The restricted feeding activates responses in hypothalamic and midbrain areas, as well as in peripheral organs involved in energy metabolism.. A restricted feeding schedule (RFS) is associated with marked behavioral arousal coincident with the food anticipatory activity (FAA) and extreme hyperphagia during food access. Article Title: Elicitor induced defence responses in medicago sativa. Article Snippet: The data of Fig. 5a–f were analysed with a Binding Assay:Article Title: Use of microproteins as tryptase inhibitors Article Snippet: For calculation of Kiapp-values, vmax values were determined and the curve fitting tool of SigmaPlot (Jandel Scientific) was applied on the basis of the following formula, that describes the case of competitive tight binding inhibitors (Morrison, 1969): v 1 v 0 = 1 - [ E t ] + [ I t ] + K iapp - { ( [ E t ] + [ I t ] + K iapp ) 2 - 4 [ E t ] · [ I t ] } 1 2 2 [ E t ] vi=vmax value of inhibited reaction v0=vmax value of uninhibited reaction [Et]=total enzyme concentration (free and in complex) [It]=total inhibitor concentration (free and in complex) Kiapp=apparent dissociation constant of the enzyme-inhibitor complex. .. For calculation of Kiapp-values, vmax values were determined and the |