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fluorescein isothiocyanate fitc  (ProSci Incorporated)


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    Structured Review

    ProSci Incorporated fluorescein isothiocyanate fitc
    Fluorescein Isothiocyanate Fitc, supplied by ProSci Incorporated, used in various techniques. Bioz Stars score: 90/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/curve+fitting+function+in+sigmaplot+software/Fluorescein+Antibody+%5B4-4-20+(enhanced)%5D%2C+Rat+IgG2c/pmc04086451-124-47-35
    Average 90 stars, based on 9 article reviews
    fluorescein isothiocyanate fitc - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    Expressing:

    Article Title: CHRONIC ALLERGEN CHALLENGE INDUCES PULMONARY EXTRAMEDULLARY HEMATOPOIESIS
    Article Snippet: .. Surface expression of CXCR4, the SDF-1 receptor, on enriched HSPCs isolated from pooled BM of chronic allergen-challenged or control mice was determined using a rabbit polyclonal CXCR4 antibody (4 μg/10 6 cells) against murine CXCR4 (ProSci, Poway, CA) in the presence of isotype-matched control antibodies followed by fluorescein isothiocyanate (FITC)-conjugated anti rabbit IgG (Sigma Chemical, St Louis, MO) on a FACSCanto II or FACScan flow cytometer equipped with FlowJo or CellQuest Pro flow cytometry analysis software (BD Biosciences). ..

    Isolation:

    Article Title: CHRONIC ALLERGEN CHALLENGE INDUCES PULMONARY EXTRAMEDULLARY HEMATOPOIESIS
    Article Snippet: .. Surface expression of CXCR4, the SDF-1 receptor, on enriched HSPCs isolated from pooled BM of chronic allergen-challenged or control mice was determined using a rabbit polyclonal CXCR4 antibody (4 μg/10 6 cells) against murine CXCR4 (ProSci, Poway, CA) in the presence of isotype-matched control antibodies followed by fluorescein isothiocyanate (FITC)-conjugated anti rabbit IgG (Sigma Chemical, St Louis, MO) on a FACSCanto II or FACScan flow cytometer equipped with FlowJo or CellQuest Pro flow cytometry analysis software (BD Biosciences). ..

    Control:

    Article Title: CHRONIC ALLERGEN CHALLENGE INDUCES PULMONARY EXTRAMEDULLARY HEMATOPOIESIS
    Article Snippet: .. Surface expression of CXCR4, the SDF-1 receptor, on enriched HSPCs isolated from pooled BM of chronic allergen-challenged or control mice was determined using a rabbit polyclonal CXCR4 antibody (4 μg/10 6 cells) against murine CXCR4 (ProSci, Poway, CA) in the presence of isotype-matched control antibodies followed by fluorescein isothiocyanate (FITC)-conjugated anti rabbit IgG (Sigma Chemical, St Louis, MO) on a FACSCanto II or FACScan flow cytometer equipped with FlowJo or CellQuest Pro flow cytometry analysis software (BD Biosciences). ..

    Flow Cytometry:

    Article Title: CHRONIC ALLERGEN CHALLENGE INDUCES PULMONARY EXTRAMEDULLARY HEMATOPOIESIS
    Article Snippet: .. Surface expression of CXCR4, the SDF-1 receptor, on enriched HSPCs isolated from pooled BM of chronic allergen-challenged or control mice was determined using a rabbit polyclonal CXCR4 antibody (4 μg/10 6 cells) against murine CXCR4 (ProSci, Poway, CA) in the presence of isotype-matched control antibodies followed by fluorescein isothiocyanate (FITC)-conjugated anti rabbit IgG (Sigma Chemical, St Louis, MO) on a FACSCanto II or FACScan flow cytometer equipped with FlowJo or CellQuest Pro flow cytometry analysis software (BD Biosciences). ..

    Software:

    Article Title: CHRONIC ALLERGEN CHALLENGE INDUCES PULMONARY EXTRAMEDULLARY HEMATOPOIESIS
    Article Snippet: .. Surface expression of CXCR4, the SDF-1 receptor, on enriched HSPCs isolated from pooled BM of chronic allergen-challenged or control mice was determined using a rabbit polyclonal CXCR4 antibody (4 μg/10 6 cells) against murine CXCR4 (ProSci, Poway, CA) in the presence of isotype-matched control antibodies followed by fluorescein isothiocyanate (FITC)-conjugated anti rabbit IgG (Sigma Chemical, St Louis, MO) on a FACSCanto II or FACScan flow cytometer equipped with FlowJo or CellQuest Pro flow cytometry analysis software (BD Biosciences). ..

    Incubation:

    Article Title: HIV-1 Gag-Virus-Like Particles Inhibit HIV-1 Replication in Dendritic Cells and T Cells through IFN-α-Dependent Upregulation of APOBEC3G and 3F
    Article Snippet: .. Cells were then incubated for 1 h with rabbit polyclonal anti-A3G (ProSci) or goat polyclonal anti-A3F (Santa Cruz) antibodies (1:500 dilution), followed by FITC-conjugated anti-rabbit IgG or anti-goat IgG (Santa Cruz), respectively. .. Nuclei were stained with DAPI (Sigma).

    Article Title: Anti-Severe Acute Respiratory Syndrome Coronavirus Spike Antibodies Trigger Infection of Human Immune Cells via a pH- and Cysteine Protease-Independent Fc?R Pathway
    Article Snippet: .. To assess SARS-CoV infection, both VeroE6 and Raji cells were incubated for 45 min with either a mouse monoclonal antibody specific for the viral nucleoprotein (N) ( 7 ) or rabbit polyclonal antibodies recognizing the viral membrane (M) protein (ProSci), which were revealed by secondary TRITC (tetramethyl rhodamine isothiocyanate)-conjugated goat anti-mouse (Zymed Laboratories) and fluorescein isothiocyanate (FITC)-conjugated goat anti-rabbit antibody (Jackson Immunoresearch), respectively. .. Slides were assembled with DAPI (4′,6′-diamidino-2-phenylindole)-containing mounting reagent (Southern Biotech) and analyzed with an AxioObserver Z1 microscope (Zeiss).

    Article Title: A20 Deficiency in Lung Epithelial Cells Protects against Influenza A Virus Infection
    Article Snippet: Tissue sections were counterstained with Mayer’s haematoxylin, rehydrated and mounted in Pertex mounting medium (Histolab). .. For A20 staining, sections were incubated for 60 min at 4°C in blocking buffer with anti-A20 (ProSci) antibody, and subsequently with HRP-labeled anti-rabbit IgG and FITC-labeled anti-HRP. .. For CCL2/MCP1 staining, sections were incubated for 30 min with anti-CCL2 (Abcam, clone ECE.2) antibody, followed by Cy3-labeled anti-rat antibody.

    Infection:

    Article Title: Anti-Severe Acute Respiratory Syndrome Coronavirus Spike Antibodies Trigger Infection of Human Immune Cells via a pH- and Cysteine Protease-Independent Fc?R Pathway
    Article Snippet: .. To assess SARS-CoV infection, both VeroE6 and Raji cells were incubated for 45 min with either a mouse monoclonal antibody specific for the viral nucleoprotein (N) ( 7 ) or rabbit polyclonal antibodies recognizing the viral membrane (M) protein (ProSci), which were revealed by secondary TRITC (tetramethyl rhodamine isothiocyanate)-conjugated goat anti-mouse (Zymed Laboratories) and fluorescein isothiocyanate (FITC)-conjugated goat anti-rabbit antibody (Jackson Immunoresearch), respectively. .. Slides were assembled with DAPI (4′,6′-diamidino-2-phenylindole)-containing mounting reagent (Southern Biotech) and analyzed with an AxioObserver Z1 microscope (Zeiss).

    Membrane:

    Article Title: Anti-Severe Acute Respiratory Syndrome Coronavirus Spike Antibodies Trigger Infection of Human Immune Cells via a pH- and Cysteine Protease-Independent Fc?R Pathway
    Article Snippet: .. To assess SARS-CoV infection, both VeroE6 and Raji cells were incubated for 45 min with either a mouse monoclonal antibody specific for the viral nucleoprotein (N) ( 7 ) or rabbit polyclonal antibodies recognizing the viral membrane (M) protein (ProSci), which were revealed by secondary TRITC (tetramethyl rhodamine isothiocyanate)-conjugated goat anti-mouse (Zymed Laboratories) and fluorescein isothiocyanate (FITC)-conjugated goat anti-rabbit antibody (Jackson Immunoresearch), respectively. .. Slides were assembled with DAPI (4′,6′-diamidino-2-phenylindole)-containing mounting reagent (Southern Biotech) and analyzed with an AxioObserver Z1 microscope (Zeiss).

    Staining:

    Article Title: A20 Deficiency in Lung Epithelial Cells Protects against Influenza A Virus Infection
    Article Snippet: Tissue sections were counterstained with Mayer’s haematoxylin, rehydrated and mounted in Pertex mounting medium (Histolab). .. For A20 staining, sections were incubated for 60 min at 4°C in blocking buffer with anti-A20 (ProSci) antibody, and subsequently with HRP-labeled anti-rabbit IgG and FITC-labeled anti-HRP. .. For CCL2/MCP1 staining, sections were incubated for 30 min with anti-CCL2 (Abcam, clone ECE.2) antibody, followed by Cy3-labeled anti-rat antibody.

    Blocking Assay:

    Article Title: A20 Deficiency in Lung Epithelial Cells Protects against Influenza A Virus Infection
    Article Snippet: Tissue sections were counterstained with Mayer’s haematoxylin, rehydrated and mounted in Pertex mounting medium (Histolab). .. For A20 staining, sections were incubated for 60 min at 4°C in blocking buffer with anti-A20 (ProSci) antibody, and subsequently with HRP-labeled anti-rabbit IgG and FITC-labeled anti-HRP. .. For CCL2/MCP1 staining, sections were incubated for 30 min with anti-CCL2 (Abcam, clone ECE.2) antibody, followed by Cy3-labeled anti-rat antibody.



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