Review



css sorting solution  (TaKaRa)


Bioz Verified Symbol TaKaRa is a verified supplier
Bioz Manufacturer Symbol TaKaRa manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
    • 97
      Buy from Supplier

    Structured Review

    TaKaRa css sorting solution
    Css Sorting Solution, supplied by TaKaRa, used in various techniques. Bioz Stars score: 97/100, based on 3113 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/css+sorting+solution/Lysis+Buffer/pmc13056611-91-10-15
    Average 97 stars, based on 3113 article reviews
    css sorting solution - by Bioz Stars, 2026-10
    97/100 stars

    Images

    Related Articles

    Polymerase Chain Reaction:

    Article Title: Protocol for purifying and sequencing cell subpopulations based on RNA FISH signal in planaria.
    Article Snippet: .. Load desired wells of a 96well skirted PCR plate with an appropriate collection buffer (e.g., SMART-Seq mRNA kit (Takara Bio) lysis buffer). ..

    Article Title: Constitutive interferon epsilon expression shapes antiviral epithelial states in the female reproductive tract and intestine.
    Article Snippet: .. Geno typing of the resulting pups was carried out by extracting genomic DNA from toe biopsies using a lysis buffer containing 50 mM Tris-base (pH 8.0), 50 mM KCl, 2.5 mM EDTA, 0.45% IGEPAL CA-630 (NP40), 0.45% Tween-20, and 20 mg/mL proteinase K, followed by incubation at 94°C for 10 min. PCR amplification was performed with primers flanking the gRNA target sites (forward: TCCCAGAACTGGAGTGGT; reverse: AA GAGCCAACAGGGGATTT) using TITANIUM Taq DNA Polymerase (Takara). ..

    Lysis:

    Article Title: Protocol for purifying and sequencing cell subpopulations based on RNA FISH signal in planaria.
    Article Snippet: .. Load desired wells of a 96well skirted PCR plate with an appropriate collection buffer (e.g., SMART-Seq mRNA kit (Takara Bio) lysis buffer). ..

    Article Title: Constitutive interferon epsilon expression shapes antiviral epithelial states in the female reproductive tract and intestine.
    Article Snippet: .. Geno typing of the resulting pups was carried out by extracting genomic DNA from toe biopsies using a lysis buffer containing 50 mM Tris-base (pH 8.0), 50 mM KCl, 2.5 mM EDTA, 0.45% IGEPAL CA-630 (NP40), 0.45% Tween-20, and 20 mg/mL proteinase K, followed by incubation at 94°C for 10 min. PCR amplification was performed with primers flanking the gRNA target sites (forward: TCCCAGAACTGGAGTGGT; reverse: AA GAGCCAACAGGGGATTT) using TITANIUM Taq DNA Polymerase (Takara). ..

    Article Title: Heterogeneous distribution of avian malaria parasites across mosquito species: disproportionate involvement of Culex restuans in the Midwestern United States.
    Article Snippet: .. Briefly, pooled mosquitoes were homogenized in 960 μL Takara LBP lysis buffer (NucleoSpin 96 DNA RapidLyse Kit, Takara Bio, Japan) supplemented with 40 μL 1 M DTT and 20 μL liquid Proteinase K. Homogenization was performed using a TissueLyser system (Qiagen, Hilden, Germany) at 30 Hz for two 5-min cycles, with the rack orientation changed between cycles, using five stainless steel beads per tube. .. Following centrifugation at 6,000 rpm for 10 min, lysates were incubated at 56°C for 10 min. A total of 200 μL of the clarified supernatant was subjected to automated nucleic acid purification using the MagMAXTM Viral/Pathogen Ultra Nucleic Acid Isolation Kit (Applied Biosystems, Thermo Fisher Scientific, Waltham, MA, USA) on a KingFisherTM Flex Magnetic Particle Processor (Thermo Fisher Scientific, Waltham, MA, USA) according to the manufacturer’s instructions.

    Article Title: Pten Orchestrates Neurogenic Radial Glia Lineage Progression and Tunes Neocortical Astrocyte Production
    Article Snippet: .. For bulk-seq, 500 astrocytes/sample were collected in 4μl cell lysis buffer [0.2% Triton X-100 and RNAse inhibitor (2 U/μl) (CloneTech)]. ..

    Article Title: Oxygen gradients reshape cross-feeding through emergent spatial organization of gut commensal bacteria
    Article Snippet: .. Cell pellets were washed and resuspended in 100 μ l of PBS, and 1 μ l of a 1:20 dilution was dispensed in a 48-well plate pre-filled with 0.26 μ l PBS, 0.26 μ l lysis buffer (Takara), 0.1 U lysozyme (50 U/ μ l; Epicentre), 0.03 μ l RNase inhibitor (100 U/ μ l; Takara), and 1.95 μ l H 2 O. .. The plate was then sealed with an aluminum-based microfilm (Bio-Rad seal F), sonicated for 20 seconds (Sonorex Digitec DT 52, Bendelin), and spun down.

    Article Title: RegVelo: Gene-regulatory-informed dynamics of single cells.
    Article Snippet: To generate full-transcript-length, deep scRNA-Seq data, we prepared the Smart-seq3 libraries following the protocol.99 During FACS, cells were sorted into individual wells containing 3-μl lysis buffer dispensed by the MANTIS liquid handler (FORMULATRIX). .. The lysis buffer consisted of 5% polyethylene glycol 8000 (Sigma), 0.1% Triton X-100 (Sigma), 0.5 U/μl RNAse inhibitor (TaKaRa), 0.5 μM OligoT30VN (IDT), 0.5 mM/each dNTPs (Thermo Fisher) and nuclease-free water (Thermo Fisher). ..

    Article Title: Shifting IRES versus Cap-initiated translation during homeostatic stem cell differentiation and stress
    Article Snippet: Colony size was measured by ImageJ (NIH) on images taken by a BX41 phase contrast microscope (Olympus) using an Infinity2 microscopy camera (Lumenera). .. To compare the transcriptome of HSPCs based on their relative use of IRES/Cap, 100 SLAM-LKS, GMP, or MEP per well were sorted on the basis of IRES/Cap from the Translator mouse into 2.6 μl of Lysis Buffer (Takara Bio USA Inc.) followed by snap freezing at −80°C in preparation for cDNA synthesis using the SMART-Seq v4 assay. ..

    Article Title: Whole-protein screening and multi-modal profiling of antigen-specific CD4 + T cells at single-cell resolution
    Article Snippet: SCTs were de-glycosylated according to the manufacturer’s protocol (NEB, P0704). .. The cells were single-cell sorted into 96-well plates containing 12 uL of 1x lysis buffer (Takara, 635013) with RNAse inhibitor (Promega, N2515). ..

    Incubation:

    Article Title: Constitutive interferon epsilon expression shapes antiviral epithelial states in the female reproductive tract and intestine.
    Article Snippet: .. Geno typing of the resulting pups was carried out by extracting genomic DNA from toe biopsies using a lysis buffer containing 50 mM Tris-base (pH 8.0), 50 mM KCl, 2.5 mM EDTA, 0.45% IGEPAL CA-630 (NP40), 0.45% Tween-20, and 20 mg/mL proteinase K, followed by incubation at 94°C for 10 min. PCR amplification was performed with primers flanking the gRNA target sites (forward: TCCCAGAACTGGAGTGGT; reverse: AA GAGCCAACAGGGGATTT) using TITANIUM Taq DNA Polymerase (Takara). ..

    Amplification:

    Article Title: Constitutive interferon epsilon expression shapes antiviral epithelial states in the female reproductive tract and intestine.
    Article Snippet: .. Geno typing of the resulting pups was carried out by extracting genomic DNA from toe biopsies using a lysis buffer containing 50 mM Tris-base (pH 8.0), 50 mM KCl, 2.5 mM EDTA, 0.45% IGEPAL CA-630 (NP40), 0.45% Tween-20, and 20 mg/mL proteinase K, followed by incubation at 94°C for 10 min. PCR amplification was performed with primers flanking the gRNA target sites (forward: TCCCAGAACTGGAGTGGT; reverse: AA GAGCCAACAGGGGATTT) using TITANIUM Taq DNA Polymerase (Takara). ..

    Homogenization:

    Article Title: Heterogeneous distribution of avian malaria parasites across mosquito species: disproportionate involvement of Culex restuans in the Midwestern United States.
    Article Snippet: .. Briefly, pooled mosquitoes were homogenized in 960 μL Takara LBP lysis buffer (NucleoSpin 96 DNA RapidLyse Kit, Takara Bio, Japan) supplemented with 40 μL 1 M DTT and 20 μL liquid Proteinase K. Homogenization was performed using a TissueLyser system (Qiagen, Hilden, Germany) at 30 Hz for two 5-min cycles, with the rack orientation changed between cycles, using five stainless steel beads per tube. .. Following centrifugation at 6,000 rpm for 10 min, lysates were incubated at 56°C for 10 min. A total of 200 μL of the clarified supernatant was subjected to automated nucleic acid purification using the MagMAXTM Viral/Pathogen Ultra Nucleic Acid Isolation Kit (Applied Biosystems, Thermo Fisher Scientific, Waltham, MA, USA) on a KingFisherTM Flex Magnetic Particle Processor (Thermo Fisher Scientific, Waltham, MA, USA) according to the manufacturer’s instructions.

    cDNA Synthesis:

    Article Title: Shifting IRES versus Cap-initiated translation during homeostatic stem cell differentiation and stress
    Article Snippet: Colony size was measured by ImageJ (NIH) on images taken by a BX41 phase contrast microscope (Olympus) using an Infinity2 microscopy camera (Lumenera). .. To compare the transcriptome of HSPCs based on their relative use of IRES/Cap, 100 SLAM-LKS, GMP, or MEP per well were sorted on the basis of IRES/Cap from the Translator mouse into 2.6 μl of Lysis Buffer (Takara Bio USA Inc.) followed by snap freezing at −80°C in preparation for cDNA synthesis using the SMART-Seq v4 assay. ..

    Single Cell:

    Article Title: Whole-protein screening and multi-modal profiling of antigen-specific CD4 + T cells at single-cell resolution
    Article Snippet: SCTs were de-glycosylated according to the manufacturer’s protocol (NEB, P0704). .. The cells were single-cell sorted into 96-well plates containing 12 uL of 1x lysis buffer (Takara, 635013) with RNAse inhibitor (Promega, N2515). ..



    Similar Products

    css sorting solution  (TaKaRa)
    97
    TaKaRa css sorting solution
    Css Sorting Solution, supplied by TaKaRa, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/css+sorting+solution/Lysis+Buffer/pmc13056611-91-10-15
    Average 97 stars, based on 1 article reviews
    css sorting solution - by Bioz Stars, 2026-10
    97/100 stars
    		  Buy from Supplier

    Image Search Results