Review



pmk1334 cropseq vector  (Addgene inc)


Bioz Verified Symbol Addgene inc is a verified supplier  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
    • 93
      Buy from Supplier

    Structured Review

    Addgene inc pmk1334 cropseq vector
    Pmk1334 Cropseq Vector, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 27 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cropseq+vector/pMK1334+(Plasmid+%23127965)/pm33767446-335-23-30
    Average 93 stars, based on 27 article reviews
    pmk1334 cropseq vector - by Bioz Stars, 2026-10
    93/100 stars

    Images

    Related Articles

    Amplification:

    Article Title: SPLiCR-seq: A CRISPR-Based Screening Platform for RNA splicing Identifies Novel Regulators of IRE1α-XBP1 Signaling Under ER Stress.
    Article Snippet: .. The amplified product was inserted into the pMK1334 vector by replacing the original WPRE cassette between EcoRI and SalI sites using the ClonExpress® Ultra One Step Cloning Kit AR TI CL E IN P RE SS ARTICLE IN PRESS (Vazyme, C115). pMK1334 was a gift from Martin Kampmann (Addgene plasmid # 127965 ; http://n2t.net/addgene:127965 ; RRID:Addgene_127965). sgRNA cloning sgRNAs for CRISPRi were cloned into the SPLiCR-seq vector or pLG15 via BstXI and Bpu1102I sites as previously described[31]. sgRNAs for CRISPR knockout were cloned into the pX459 vector via BsmBI sites. .. The pX459 vector was a gift from Feng Zhang (Addgene plasmid # 62988 ; http://n2t.net/addgene:62988 ; RRID:Addgene_62988).

    Article Title: SPLiCR-seq: A CRISPR-Based Screening Platform for RNA splicing Identifies Novel Regulators of IRE1α-XBP1 Signaling Under ER Stress
    Article Snippet: .. The amplified product was inserted into the pMK1334 vector by replacing the original WPRE cassette between EcoRI and SalI sites using the ClonExpress® Ultra One Step Cloning Kit (Vazyme, C115). pMK1334 was a gift from Martin Kampmann (Addgene plasmid # 127965; http://n2t.net/addgene:127965 ; RRID:Addgene_127965). ..

    Article Title: Multimodal CRISPR screens uncover DDX39B as a global repressor of A-to-I RNA editing.
    Article Snippet: .. The amplified product was instead into a CROP-seq vector pMK1334 (Addgene, 127965) by replacing original WPRE cassette between EcoRI and SalI sites using ClonExpress Ultra One Step Cloning Kit (Vazyme, C115). .. REAGENT or RESOURCE SOURCE IDENTIFIER pcDNA3.1-3×Flag-ADAR1 This paper N/A pcDNA3.1-3×Flag-ADAR2 This paper N/A pcDNA3.1-3×HA-DDX39B This paper N/A PX459 Addgene Cat#62988 HDV dual-fluorescent RNA editing reporter This paper N/A Software and algorithms MAGeCK-iNC Tian et al.33 https://kampmannlab.ucsf.edu/ mageck-inc GraphPad Prism 10 GraphPad https://www.graphpad.com/ Fiji NIH https://fiji.sc/ PyMOL Molecular Graphics System Version 3.0 Schrödinger https://pymol.org/ CellProfiler Cimini Lab https://cellprofiler.org/ STARsolo (STAR v2.7.11a) Dobin et al.87 N/A scanpy package (version 1.10.2) Wolf et al.88 N/A REDIportal Mansi et al.89 http://srv00.recas.ba.infn.it/ atlas/index.html dbSNP (version v156) Sherry et al.90 NA decoupleR (version 1.8.0) Badia et al.91 https://www.bioconductor.org/packages/ release/bioc/html/decoupleR.html PyDESeqE (version 0.4.12) Muzellec et al.92 https://github.com/owkin/PyDESeq2 SnapGene (version 8.0) SnapGene https://www.snapgene.com/ Code for scCREDITS analysis This paper https://doi.org/10.5281/zenodo.15614476 Cell Reports 44, 116009, July 22, 2025 19 Generation of CRISPRi-HEK293T cell line with the stable overexpression of ADAR2 CRISPRi-HEK293T cell line was generated as described previously.93 The cDNA of human ADARB1 was gifted from Dr. Hao Chen (SUSTech) and was subcloned together with a 3×FLAG tag into a pLV2-UBC-mCherry-Hyg-CMV-MCS vector (Miaoling, P36518) between BamHI and AgeI sites.

    Cloning:

    Article Title: SPLiCR-seq: A CRISPR-Based Screening Platform for RNA splicing Identifies Novel Regulators of IRE1α-XBP1 Signaling Under ER Stress.
    Article Snippet: .. The amplified product was inserted into the pMK1334 vector by replacing the original WPRE cassette between EcoRI and SalI sites using the ClonExpress® Ultra One Step Cloning Kit AR TI CL E IN P RE SS ARTICLE IN PRESS (Vazyme, C115). pMK1334 was a gift from Martin Kampmann (Addgene plasmid # 127965 ; http://n2t.net/addgene:127965 ; RRID:Addgene_127965). sgRNA cloning sgRNAs for CRISPRi were cloned into the SPLiCR-seq vector or pLG15 via BstXI and Bpu1102I sites as previously described[31]. sgRNAs for CRISPR knockout were cloned into the pX459 vector via BsmBI sites. .. The pX459 vector was a gift from Feng Zhang (Addgene plasmid # 62988 ; http://n2t.net/addgene:62988 ; RRID:Addgene_62988).

    Article Title: SPLiCR-seq: A CRISPR-Based Screening Platform for RNA splicing Identifies Novel Regulators of IRE1α-XBP1 Signaling Under ER Stress
    Article Snippet: .. The amplified product was inserted into the pMK1334 vector by replacing the original WPRE cassette between EcoRI and SalI sites using the ClonExpress® Ultra One Step Cloning Kit (Vazyme, C115). pMK1334 was a gift from Martin Kampmann (Addgene plasmid # 127965; http://n2t.net/addgene:127965 ; RRID:Addgene_127965). ..

    Article Title: Multimodal CRISPR screens uncover DDX39B as a global repressor of A-to-I RNA editing.
    Article Snippet: .. The amplified product was instead into a CROP-seq vector pMK1334 (Addgene, 127965) by replacing original WPRE cassette between EcoRI and SalI sites using ClonExpress Ultra One Step Cloning Kit (Vazyme, C115). .. REAGENT or RESOURCE SOURCE IDENTIFIER pcDNA3.1-3×Flag-ADAR1 This paper N/A pcDNA3.1-3×Flag-ADAR2 This paper N/A pcDNA3.1-3×HA-DDX39B This paper N/A PX459 Addgene Cat#62988 HDV dual-fluorescent RNA editing reporter This paper N/A Software and algorithms MAGeCK-iNC Tian et al.33 https://kampmannlab.ucsf.edu/ mageck-inc GraphPad Prism 10 GraphPad https://www.graphpad.com/ Fiji NIH https://fiji.sc/ PyMOL Molecular Graphics System Version 3.0 Schrödinger https://pymol.org/ CellProfiler Cimini Lab https://cellprofiler.org/ STARsolo (STAR v2.7.11a) Dobin et al.87 N/A scanpy package (version 1.10.2) Wolf et al.88 N/A REDIportal Mansi et al.89 http://srv00.recas.ba.infn.it/ atlas/index.html dbSNP (version v156) Sherry et al.90 NA decoupleR (version 1.8.0) Badia et al.91 https://www.bioconductor.org/packages/ release/bioc/html/decoupleR.html PyDESeqE (version 0.4.12) Muzellec et al.92 https://github.com/owkin/PyDESeq2 SnapGene (version 8.0) SnapGene https://www.snapgene.com/ Code for scCREDITS analysis This paper https://doi.org/10.5281/zenodo.15614476 Cell Reports 44, 116009, July 22, 2025 19 Generation of CRISPRi-HEK293T cell line with the stable overexpression of ADAR2 CRISPRi-HEK293T cell line was generated as described previously.93 The cDNA of human ADARB1 was gifted from Dr. Hao Chen (SUSTech) and was subcloned together with a 3×FLAG tag into a pLV2-UBC-mCherry-Hyg-CMV-MCS vector (Miaoling, P36518) between BamHI and AgeI sites.

    Plasmid Preparation:

    Article Title: SPLiCR-seq: A CRISPR-Based Screening Platform for RNA splicing Identifies Novel Regulators of IRE1α-XBP1 Signaling Under ER Stress.
    Article Snippet: .. The amplified product was inserted into the pMK1334 vector by replacing the original WPRE cassette between EcoRI and SalI sites using the ClonExpress® Ultra One Step Cloning Kit AR TI CL E IN P RE SS ARTICLE IN PRESS (Vazyme, C115). pMK1334 was a gift from Martin Kampmann (Addgene plasmid # 127965 ; http://n2t.net/addgene:127965 ; RRID:Addgene_127965). sgRNA cloning sgRNAs for CRISPRi were cloned into the SPLiCR-seq vector or pLG15 via BstXI and Bpu1102I sites as previously described[31]. sgRNAs for CRISPR knockout were cloned into the pX459 vector via BsmBI sites. .. The pX459 vector was a gift from Feng Zhang (Addgene plasmid # 62988 ; http://n2t.net/addgene:62988 ; RRID:Addgene_62988).

    Article Title: SPLiCR-seq: A CRISPR-Based Screening Platform for RNA splicing Identifies Novel Regulators of IRE1α-XBP1 Signaling Under ER Stress
    Article Snippet: .. The amplified product was inserted into the pMK1334 vector by replacing the original WPRE cassette between EcoRI and SalI sites using the ClonExpress® Ultra One Step Cloning Kit (Vazyme, C115). pMK1334 was a gift from Martin Kampmann (Addgene plasmid # 127965; http://n2t.net/addgene:127965 ; RRID:Addgene_127965). ..

    Article Title: Multimodal CRISPR screens uncover DDX39B as a global repressor of A-to-I RNA editing.
    Article Snippet: .. The amplified product was instead into a CROP-seq vector pMK1334 (Addgene, 127965) by replacing original WPRE cassette between EcoRI and SalI sites using ClonExpress Ultra One Step Cloning Kit (Vazyme, C115). .. REAGENT or RESOURCE SOURCE IDENTIFIER pcDNA3.1-3×Flag-ADAR1 This paper N/A pcDNA3.1-3×Flag-ADAR2 This paper N/A pcDNA3.1-3×HA-DDX39B This paper N/A PX459 Addgene Cat#62988 HDV dual-fluorescent RNA editing reporter This paper N/A Software and algorithms MAGeCK-iNC Tian et al.33 https://kampmannlab.ucsf.edu/ mageck-inc GraphPad Prism 10 GraphPad https://www.graphpad.com/ Fiji NIH https://fiji.sc/ PyMOL Molecular Graphics System Version 3.0 Schrödinger https://pymol.org/ CellProfiler Cimini Lab https://cellprofiler.org/ STARsolo (STAR v2.7.11a) Dobin et al.87 N/A scanpy package (version 1.10.2) Wolf et al.88 N/A REDIportal Mansi et al.89 http://srv00.recas.ba.infn.it/ atlas/index.html dbSNP (version v156) Sherry et al.90 NA decoupleR (version 1.8.0) Badia et al.91 https://www.bioconductor.org/packages/ release/bioc/html/decoupleR.html PyDESeqE (version 0.4.12) Muzellec et al.92 https://github.com/owkin/PyDESeq2 SnapGene (version 8.0) SnapGene https://www.snapgene.com/ Code for scCREDITS analysis This paper https://doi.org/10.5281/zenodo.15614476 Cell Reports 44, 116009, July 22, 2025 19 Generation of CRISPRi-HEK293T cell line with the stable overexpression of ADAR2 CRISPRi-HEK293T cell line was generated as described previously.93 The cDNA of human ADARB1 was gifted from Dr. Hao Chen (SUSTech) and was subcloned together with a 3×FLAG tag into a pLV2-UBC-mCherry-Hyg-CMV-MCS vector (Miaoling, P36518) between BamHI and AgeI sites.

    Clone Assay:

    Article Title: SPLiCR-seq: A CRISPR-Based Screening Platform for RNA splicing Identifies Novel Regulators of IRE1α-XBP1 Signaling Under ER Stress.
    Article Snippet: .. The amplified product was inserted into the pMK1334 vector by replacing the original WPRE cassette between EcoRI and SalI sites using the ClonExpress® Ultra One Step Cloning Kit AR TI CL E IN P RE SS ARTICLE IN PRESS (Vazyme, C115). pMK1334 was a gift from Martin Kampmann (Addgene plasmid # 127965 ; http://n2t.net/addgene:127965 ; RRID:Addgene_127965). sgRNA cloning sgRNAs for CRISPRi were cloned into the SPLiCR-seq vector or pLG15 via BstXI and Bpu1102I sites as previously described[31]. sgRNAs for CRISPR knockout were cloned into the pX459 vector via BsmBI sites. .. The pX459 vector was a gift from Feng Zhang (Addgene plasmid # 62988 ; http://n2t.net/addgene:62988 ; RRID:Addgene_62988).

    Article Title: Regulators of Interferon-Responsive Microglia Uncovered by Genome-wide CRISPRi Screening
    Article Snippet: .. For performing the genome-wide screen, seven sublibraries (CRISPRi_v2) were cloned into the pMK1334 background (Addgene, 127965) as described previously , . ..

    CRISPR:

    Article Title: SPLiCR-seq: A CRISPR-Based Screening Platform for RNA splicing Identifies Novel Regulators of IRE1α-XBP1 Signaling Under ER Stress.
    Article Snippet: .. The amplified product was inserted into the pMK1334 vector by replacing the original WPRE cassette between EcoRI and SalI sites using the ClonExpress® Ultra One Step Cloning Kit AR TI CL E IN P RE SS ARTICLE IN PRESS (Vazyme, C115). pMK1334 was a gift from Martin Kampmann (Addgene plasmid # 127965 ; http://n2t.net/addgene:127965 ; RRID:Addgene_127965). sgRNA cloning sgRNAs for CRISPRi were cloned into the SPLiCR-seq vector or pLG15 via BstXI and Bpu1102I sites as previously described[31]. sgRNAs for CRISPR knockout were cloned into the pX459 vector via BsmBI sites. .. The pX459 vector was a gift from Feng Zhang (Addgene plasmid # 62988 ; http://n2t.net/addgene:62988 ; RRID:Addgene_62988).

    Article Title: CRISPR screens in iPSC-derived neurons reveal principles of tau proteostasis.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit anti-Actin Cell Signaling Cat#4970 Mouse anti-Actin Cell Signaling Cat#3700 Rabbit anti-GFP Chromotek Cat#pabg1 Mouse anti-GFP Santa Cruz Biotech Cat#sc-9996 Mouse anti-GAPDH Santa Cruz Biotech Cat#sc-32233 Rabbit anti-tau oligomer (T22) Rakez Kayed, The University of Texas Medical Branch at Galveston Gift Rabbit anti-tau oligomer (M204) David Eisenberg, University of California, Los Angeles N/A Mouse anti-tau oligomer (TOC1) Nicholas Kanaan, Michigan State University N/A Rabbit anti-tau oligomer (T22-I) EMD Millipore Cat#ABN454-I Rabbit anti-tau oligomer (TTC18) Li Gan, Weill Cornell Medical School Gift Mouse anti-tau (Tau13) Santa Cruz Biotech Cat#21796 Mouse anti-tau (HT7) Thermo Fisher Scientific Cat#MN1000 Rabbit anti-tau DAKO Cat#05-803 Biotin anti-tau, 6-18 antibody (Tau12) Biolegend Cat#806506 Pierce Streptavidin Poly-HRP Thermo Fisher Scientific Cat#21140 Mouse anti-NeuN Biolegend Cat#SIG-39860 Mouse anti-Rbx2 (RNF7) Santa Cruz Biotech Cat#166554 Rabbit anti-ELOB ProteinTech Cat#A0024 Rabbit anti-Histone H3 Cell Signaling Cat#4499 Rabbit anti-PSD95 Cell Signaling Cat#3450 Goat anti-rabbit 680 LICOR Cat#926-32210 Goat anti-mouse 800 LICOR Cat#926-68071 Anti-mouse IgG, HRP-linked Cell Signaling Cat#7076 Anti-rabbit IgG, HRP-linked Cell Signaling Cat#7074 Rabbit anti-FLAG Cell Signaling Cat#14793 Mouse anti-HA Cell Signaling Cat#2367 Mouse anti-PSMA2 Thermo Fisher Scientific Cat#MA5-26149 Mouse anti PSMA1-3,5-7 Abcam Cat#MCP231 Rabbit anti PSMD11 Cell Signaling Cat#14303 Rabbit anti-CUL5 Atlas Antibodies Cat#HPA002185 Bacterial or virus strains DH5alpha Thermo Fisher Scientific Cat#18265017 BL21(DE3) Thermo Fisher Scientific Cat#EC0114 MegaX DH10B T1 R Thermo Fisher Scientific Cat#C640003 Chemicals, peptides, and recombinant proteins StemFlex Media Thermo Fisher Scientific Cat#A3349401 Y-27632 Stem Cell Technologies Cat#72308 Matrigel hESC-Qualified Matrix Corning Cat#354277 Doxycycline hyclate (Dox) Sigma-Aldrich Cat#D9891 Brain-derived neurotrophic factor (BDNF) PeproTech Cat#450-02 Neurotrophin-3 (NT-3) PeproTech Cat#450-03 (Continued on next page) ll OPEN ACCESS Cell 189, 1–18.e1–e10, March 5, 2026 e1 Please cite this article in press as: Samelson et al., CRISPR screens in iPSC-derived neurons reveal principles of tau proteostasis, Cell (2026), https://doi.org/10.1016/j.cell.2025.12.038 Article .. REAGENT or RESOURCE SOURCE IDENTIFIER Zymo Gigaprep kit Zymo Cat#D4204 Poly-D-Lysine Culture Dishes Corning Cat#354550 Universal Mycoplasma Detection Kit ATCC Cat#30-1012K Deposited data Raw and processed data (bulk CRISPR-screen data) This paper N/A Experimental models: Cell lines HEK293T ATCC Cat#CRL-11268 Human WTC11 iPSC with Ngn2 transgene and CRISPRi integration Tian et al. 20 N/A Human WTC11 iPSC with Ngn2 transgene and CRISPRa integration Tian et al. 63 N/A Human WTC11 iPSC with Ngn2 transgene and CRISPRi integration WT/V337M This paper N/A Human WTC11 iPSC with Ngn2 transgene integration V337M/V337M Gilbert et al. 77 N/A Oligonucleotides Primers for sequencing; see Table S1 This paper N/A Primers for qPCR; see Table S2 This paper N/A Recombinant DNA pMD2.G Didier Trono Addgene #12259 pMDLg/pRRE Didier Trono Addgene #12251 pRSV-REV Didier Trono Addgene #12253 pC13N-CLYBL-CAG-dCas9-BFP-KRAB Martin Kampmann Addgene #127968 pMK1334 Martin Kampmann Addgene #127965 pAJS1197 Cul5-3xFlag-T2A-mApple This paper N/A pAJS1203 GFP-3xFlag-T2A-mApple This paper N/A pAJS1205 GFP-Tau-1-50-T2A-mApple This paper N/A pAJS1206 GFP-Tau-40-90-T2A-mApple This paper N/A pAJS1207 GFP-Tau-80-130-T2A-mApple This paper N/A pAJS1208 GFP-Tau-120-170-T2A-mApple This paper N/A pAJS1209 GFP-Tau-160-210-T2A-mApple This paper N/A pAJS1210 GFP-Tau-200-250-T2A-mApple This paper N/A pAJS1211 GFP-Tau-240-290-T2A-mApple This paper N/A pAJS1212 GFP-Tau-280-352-T2A-mApple This paper N/A pAJS1172 GFP-Tau-Metless This paper N/A pAJS1184 GFP-Tau-T2A-mApple This paper N/A pAJS1154 GFP This paper N/A pAJS1149 GFP-Tau This paper N/A pZTC-13-R1 Jizhong Zou Addgene #62196 pZT-C13-L1 Jizhong Zou Addgene #62197 pAJS1224 pET-Duet EloBC Gift of John Gross, University of California, San Francisco Gift pAJS1228 His-Sumo-Tau-1-172 This paper N/A pAJS1229 His-Sumo-Tau-1-176 This paper N/A pIVM_26 Alessio Ciulli Addgene #204501 pAJS1200 SOCS4-3xFlag-T2A-mApple This paper N/A pAJS1216 GFP-Tau-80-130-IRES-mApple This paper N/A (Continued on next page) ll OPEN ACCESS Cell 189, 1–18.e1–e10, March 5, 2026 e3 Please cite this article in press as: Samelson et al., CRISPR screens in iPSC-derived neurons reveal principles of tau proteostasis, Cell (2026), https://doi.org/10.1016/j.cell.2025.12.038 Article .. Human iPSCs (in the male WTC11 background 79 ) were cultured in StemFlex Medium on BioLite Cell Culture Treated Dishes (Thermo Fisher Scientific; assorted Cat. No.) coated with Growth Factor Reduced, Phenol Red-Free, LDEV-Free Ma-trigel Basement Membrane Matrix (Corning; Cat. No. 356231) diluted 1:100 in Knockout DMEM (GIBCO/Thermo Fisher Scientific; Cat. No. 10829-018).

    Knock-Out:

    Article Title: SPLiCR-seq: A CRISPR-Based Screening Platform for RNA splicing Identifies Novel Regulators of IRE1α-XBP1 Signaling Under ER Stress.
    Article Snippet: .. The amplified product was inserted into the pMK1334 vector by replacing the original WPRE cassette between EcoRI and SalI sites using the ClonExpress® Ultra One Step Cloning Kit AR TI CL E IN P RE SS ARTICLE IN PRESS (Vazyme, C115). pMK1334 was a gift from Martin Kampmann (Addgene plasmid # 127965 ; http://n2t.net/addgene:127965 ; RRID:Addgene_127965). sgRNA cloning sgRNAs for CRISPRi were cloned into the SPLiCR-seq vector or pLG15 via BstXI and Bpu1102I sites as previously described[31]. sgRNAs for CRISPR knockout were cloned into the pX459 vector via BsmBI sites. .. The pX459 vector was a gift from Feng Zhang (Addgene plasmid # 62988 ; http://n2t.net/addgene:62988 ; RRID:Addgene_62988).

    Genome Wide:

    Article Title: Regulators of Interferon-Responsive Microglia Uncovered by Genome-wide CRISPRi Screening
    Article Snippet: .. For performing the genome-wide screen, seven sublibraries (CRISPRi_v2) were cloned into the pMK1334 background (Addgene, 127965) as described previously , . ..

    Sequencing:

    Article Title: CRISPR screens in iPSC-derived neurons reveal principles of tau proteostasis.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit anti-Actin Cell Signaling Cat#4970 Mouse anti-Actin Cell Signaling Cat#3700 Rabbit anti-GFP Chromotek Cat#pabg1 Mouse anti-GFP Santa Cruz Biotech Cat#sc-9996 Mouse anti-GAPDH Santa Cruz Biotech Cat#sc-32233 Rabbit anti-tau oligomer (T22) Rakez Kayed, The University of Texas Medical Branch at Galveston Gift Rabbit anti-tau oligomer (M204) David Eisenberg, University of California, Los Angeles N/A Mouse anti-tau oligomer (TOC1) Nicholas Kanaan, Michigan State University N/A Rabbit anti-tau oligomer (T22-I) EMD Millipore Cat#ABN454-I Rabbit anti-tau oligomer (TTC18) Li Gan, Weill Cornell Medical School Gift Mouse anti-tau (Tau13) Santa Cruz Biotech Cat#21796 Mouse anti-tau (HT7) Thermo Fisher Scientific Cat#MN1000 Rabbit anti-tau DAKO Cat#05-803 Biotin anti-tau, 6-18 antibody (Tau12) Biolegend Cat#806506 Pierce Streptavidin Poly-HRP Thermo Fisher Scientific Cat#21140 Mouse anti-NeuN Biolegend Cat#SIG-39860 Mouse anti-Rbx2 (RNF7) Santa Cruz Biotech Cat#166554 Rabbit anti-ELOB ProteinTech Cat#A0024 Rabbit anti-Histone H3 Cell Signaling Cat#4499 Rabbit anti-PSD95 Cell Signaling Cat#3450 Goat anti-rabbit 680 LICOR Cat#926-32210 Goat anti-mouse 800 LICOR Cat#926-68071 Anti-mouse IgG, HRP-linked Cell Signaling Cat#7076 Anti-rabbit IgG, HRP-linked Cell Signaling Cat#7074 Rabbit anti-FLAG Cell Signaling Cat#14793 Mouse anti-HA Cell Signaling Cat#2367 Mouse anti-PSMA2 Thermo Fisher Scientific Cat#MA5-26149 Mouse anti PSMA1-3,5-7 Abcam Cat#MCP231 Rabbit anti PSMD11 Cell Signaling Cat#14303 Rabbit anti-CUL5 Atlas Antibodies Cat#HPA002185 Bacterial or virus strains DH5alpha Thermo Fisher Scientific Cat#18265017 BL21(DE3) Thermo Fisher Scientific Cat#EC0114 MegaX DH10B T1 R Thermo Fisher Scientific Cat#C640003 Chemicals, peptides, and recombinant proteins StemFlex Media Thermo Fisher Scientific Cat#A3349401 Y-27632 Stem Cell Technologies Cat#72308 Matrigel hESC-Qualified Matrix Corning Cat#354277 Doxycycline hyclate (Dox) Sigma-Aldrich Cat#D9891 Brain-derived neurotrophic factor (BDNF) PeproTech Cat#450-02 Neurotrophin-3 (NT-3) PeproTech Cat#450-03 (Continued on next page) ll OPEN ACCESS Cell 189, 1–18.e1–e10, March 5, 2026 e1 Please cite this article in press as: Samelson et al., CRISPR screens in iPSC-derived neurons reveal principles of tau proteostasis, Cell (2026), https://doi.org/10.1016/j.cell.2025.12.038 Article .. REAGENT or RESOURCE SOURCE IDENTIFIER Zymo Gigaprep kit Zymo Cat#D4204 Poly-D-Lysine Culture Dishes Corning Cat#354550 Universal Mycoplasma Detection Kit ATCC Cat#30-1012K Deposited data Raw and processed data (bulk CRISPR-screen data) This paper N/A Experimental models: Cell lines HEK293T ATCC Cat#CRL-11268 Human WTC11 iPSC with Ngn2 transgene and CRISPRi integration Tian et al. 20 N/A Human WTC11 iPSC with Ngn2 transgene and CRISPRa integration Tian et al. 63 N/A Human WTC11 iPSC with Ngn2 transgene and CRISPRi integration WT/V337M This paper N/A Human WTC11 iPSC with Ngn2 transgene integration V337M/V337M Gilbert et al. 77 N/A Oligonucleotides Primers for sequencing; see Table S1 This paper N/A Primers for qPCR; see Table S2 This paper N/A Recombinant DNA pMD2.G Didier Trono Addgene #12259 pMDLg/pRRE Didier Trono Addgene #12251 pRSV-REV Didier Trono Addgene #12253 pC13N-CLYBL-CAG-dCas9-BFP-KRAB Martin Kampmann Addgene #127968 pMK1334 Martin Kampmann Addgene #127965 pAJS1197 Cul5-3xFlag-T2A-mApple This paper N/A pAJS1203 GFP-3xFlag-T2A-mApple This paper N/A pAJS1205 GFP-Tau-1-50-T2A-mApple This paper N/A pAJS1206 GFP-Tau-40-90-T2A-mApple This paper N/A pAJS1207 GFP-Tau-80-130-T2A-mApple This paper N/A pAJS1208 GFP-Tau-120-170-T2A-mApple This paper N/A pAJS1209 GFP-Tau-160-210-T2A-mApple This paper N/A pAJS1210 GFP-Tau-200-250-T2A-mApple This paper N/A pAJS1211 GFP-Tau-240-290-T2A-mApple This paper N/A pAJS1212 GFP-Tau-280-352-T2A-mApple This paper N/A pAJS1172 GFP-Tau-Metless This paper N/A pAJS1184 GFP-Tau-T2A-mApple This paper N/A pAJS1154 GFP This paper N/A pAJS1149 GFP-Tau This paper N/A pZTC-13-R1 Jizhong Zou Addgene #62196 pZT-C13-L1 Jizhong Zou Addgene #62197 pAJS1224 pET-Duet EloBC Gift of John Gross, University of California, San Francisco Gift pAJS1228 His-Sumo-Tau-1-172 This paper N/A pAJS1229 His-Sumo-Tau-1-176 This paper N/A pIVM_26 Alessio Ciulli Addgene #204501 pAJS1200 SOCS4-3xFlag-T2A-mApple This paper N/A pAJS1216 GFP-Tau-80-130-IRES-mApple This paper N/A (Continued on next page) ll OPEN ACCESS Cell 189, 1–18.e1–e10, March 5, 2026 e3 Please cite this article in press as: Samelson et al., CRISPR screens in iPSC-derived neurons reveal principles of tau proteostasis, Cell (2026), https://doi.org/10.1016/j.cell.2025.12.038 Article .. Human iPSCs (in the male WTC11 background 79 ) were cultured in StemFlex Medium on BioLite Cell Culture Treated Dishes (Thermo Fisher Scientific; assorted Cat. No.) coated with Growth Factor Reduced, Phenol Red-Free, LDEV-Free Ma-trigel Basement Membrane Matrix (Corning; Cat. No. 356231) diluted 1:100 in Knockout DMEM (GIBCO/Thermo Fisher Scientific; Cat. No. 10829-018).

    Real-time Polymerase Chain Reaction:

    Article Title: CRISPR screens in iPSC-derived neurons reveal principles of tau proteostasis.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit anti-Actin Cell Signaling Cat#4970 Mouse anti-Actin Cell Signaling Cat#3700 Rabbit anti-GFP Chromotek Cat#pabg1 Mouse anti-GFP Santa Cruz Biotech Cat#sc-9996 Mouse anti-GAPDH Santa Cruz Biotech Cat#sc-32233 Rabbit anti-tau oligomer (T22) Rakez Kayed, The University of Texas Medical Branch at Galveston Gift Rabbit anti-tau oligomer (M204) David Eisenberg, University of California, Los Angeles N/A Mouse anti-tau oligomer (TOC1) Nicholas Kanaan, Michigan State University N/A Rabbit anti-tau oligomer (T22-I) EMD Millipore Cat#ABN454-I Rabbit anti-tau oligomer (TTC18) Li Gan, Weill Cornell Medical School Gift Mouse anti-tau (Tau13) Santa Cruz Biotech Cat#21796 Mouse anti-tau (HT7) Thermo Fisher Scientific Cat#MN1000 Rabbit anti-tau DAKO Cat#05-803 Biotin anti-tau, 6-18 antibody (Tau12) Biolegend Cat#806506 Pierce Streptavidin Poly-HRP Thermo Fisher Scientific Cat#21140 Mouse anti-NeuN Biolegend Cat#SIG-39860 Mouse anti-Rbx2 (RNF7) Santa Cruz Biotech Cat#166554 Rabbit anti-ELOB ProteinTech Cat#A0024 Rabbit anti-Histone H3 Cell Signaling Cat#4499 Rabbit anti-PSD95 Cell Signaling Cat#3450 Goat anti-rabbit 680 LICOR Cat#926-32210 Goat anti-mouse 800 LICOR Cat#926-68071 Anti-mouse IgG, HRP-linked Cell Signaling Cat#7076 Anti-rabbit IgG, HRP-linked Cell Signaling Cat#7074 Rabbit anti-FLAG Cell Signaling Cat#14793 Mouse anti-HA Cell Signaling Cat#2367 Mouse anti-PSMA2 Thermo Fisher Scientific Cat#MA5-26149 Mouse anti PSMA1-3,5-7 Abcam Cat#MCP231 Rabbit anti PSMD11 Cell Signaling Cat#14303 Rabbit anti-CUL5 Atlas Antibodies Cat#HPA002185 Bacterial or virus strains DH5alpha Thermo Fisher Scientific Cat#18265017 BL21(DE3) Thermo Fisher Scientific Cat#EC0114 MegaX DH10B T1 R Thermo Fisher Scientific Cat#C640003 Chemicals, peptides, and recombinant proteins StemFlex Media Thermo Fisher Scientific Cat#A3349401 Y-27632 Stem Cell Technologies Cat#72308 Matrigel hESC-Qualified Matrix Corning Cat#354277 Doxycycline hyclate (Dox) Sigma-Aldrich Cat#D9891 Brain-derived neurotrophic factor (BDNF) PeproTech Cat#450-02 Neurotrophin-3 (NT-3) PeproTech Cat#450-03 (Continued on next page) ll OPEN ACCESS Cell 189, 1–18.e1–e10, March 5, 2026 e1 Please cite this article in press as: Samelson et al., CRISPR screens in iPSC-derived neurons reveal principles of tau proteostasis, Cell (2026), https://doi.org/10.1016/j.cell.2025.12.038 Article .. REAGENT or RESOURCE SOURCE IDENTIFIER Zymo Gigaprep kit Zymo Cat#D4204 Poly-D-Lysine Culture Dishes Corning Cat#354550 Universal Mycoplasma Detection Kit ATCC Cat#30-1012K Deposited data Raw and processed data (bulk CRISPR-screen data) This paper N/A Experimental models: Cell lines HEK293T ATCC Cat#CRL-11268 Human WTC11 iPSC with Ngn2 transgene and CRISPRi integration Tian et al. 20 N/A Human WTC11 iPSC with Ngn2 transgene and CRISPRa integration Tian et al. 63 N/A Human WTC11 iPSC with Ngn2 transgene and CRISPRi integration WT/V337M This paper N/A Human WTC11 iPSC with Ngn2 transgene integration V337M/V337M Gilbert et al. 77 N/A Oligonucleotides Primers for sequencing; see Table S1 This paper N/A Primers for qPCR; see Table S2 This paper N/A Recombinant DNA pMD2.G Didier Trono Addgene #12259 pMDLg/pRRE Didier Trono Addgene #12251 pRSV-REV Didier Trono Addgene #12253 pC13N-CLYBL-CAG-dCas9-BFP-KRAB Martin Kampmann Addgene #127968 pMK1334 Martin Kampmann Addgene #127965 pAJS1197 Cul5-3xFlag-T2A-mApple This paper N/A pAJS1203 GFP-3xFlag-T2A-mApple This paper N/A pAJS1205 GFP-Tau-1-50-T2A-mApple This paper N/A pAJS1206 GFP-Tau-40-90-T2A-mApple This paper N/A pAJS1207 GFP-Tau-80-130-T2A-mApple This paper N/A pAJS1208 GFP-Tau-120-170-T2A-mApple This paper N/A pAJS1209 GFP-Tau-160-210-T2A-mApple This paper N/A pAJS1210 GFP-Tau-200-250-T2A-mApple This paper N/A pAJS1211 GFP-Tau-240-290-T2A-mApple This paper N/A pAJS1212 GFP-Tau-280-352-T2A-mApple This paper N/A pAJS1172 GFP-Tau-Metless This paper N/A pAJS1184 GFP-Tau-T2A-mApple This paper N/A pAJS1154 GFP This paper N/A pAJS1149 GFP-Tau This paper N/A pZTC-13-R1 Jizhong Zou Addgene #62196 pZT-C13-L1 Jizhong Zou Addgene #62197 pAJS1224 pET-Duet EloBC Gift of John Gross, University of California, San Francisco Gift pAJS1228 His-Sumo-Tau-1-172 This paper N/A pAJS1229 His-Sumo-Tau-1-176 This paper N/A pIVM_26 Alessio Ciulli Addgene #204501 pAJS1200 SOCS4-3xFlag-T2A-mApple This paper N/A pAJS1216 GFP-Tau-80-130-IRES-mApple This paper N/A (Continued on next page) ll OPEN ACCESS Cell 189, 1–18.e1–e10, March 5, 2026 e3 Please cite this article in press as: Samelson et al., CRISPR screens in iPSC-derived neurons reveal principles of tau proteostasis, Cell (2026), https://doi.org/10.1016/j.cell.2025.12.038 Article .. Human iPSCs (in the male WTC11 background 79 ) were cultured in StemFlex Medium on BioLite Cell Culture Treated Dishes (Thermo Fisher Scientific; assorted Cat. No.) coated with Growth Factor Reduced, Phenol Red-Free, LDEV-Free Ma-trigel Basement Membrane Matrix (Corning; Cat. No. 356231) diluted 1:100 in Knockout DMEM (GIBCO/Thermo Fisher Scientific; Cat. No. 10829-018).

    Recombinant:

    Article Title: CRISPR screens in iPSC-derived neurons reveal principles of tau proteostasis.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit anti-Actin Cell Signaling Cat#4970 Mouse anti-Actin Cell Signaling Cat#3700 Rabbit anti-GFP Chromotek Cat#pabg1 Mouse anti-GFP Santa Cruz Biotech Cat#sc-9996 Mouse anti-GAPDH Santa Cruz Biotech Cat#sc-32233 Rabbit anti-tau oligomer (T22) Rakez Kayed, The University of Texas Medical Branch at Galveston Gift Rabbit anti-tau oligomer (M204) David Eisenberg, University of California, Los Angeles N/A Mouse anti-tau oligomer (TOC1) Nicholas Kanaan, Michigan State University N/A Rabbit anti-tau oligomer (T22-I) EMD Millipore Cat#ABN454-I Rabbit anti-tau oligomer (TTC18) Li Gan, Weill Cornell Medical School Gift Mouse anti-tau (Tau13) Santa Cruz Biotech Cat#21796 Mouse anti-tau (HT7) Thermo Fisher Scientific Cat#MN1000 Rabbit anti-tau DAKO Cat#05-803 Biotin anti-tau, 6-18 antibody (Tau12) Biolegend Cat#806506 Pierce Streptavidin Poly-HRP Thermo Fisher Scientific Cat#21140 Mouse anti-NeuN Biolegend Cat#SIG-39860 Mouse anti-Rbx2 (RNF7) Santa Cruz Biotech Cat#166554 Rabbit anti-ELOB ProteinTech Cat#A0024 Rabbit anti-Histone H3 Cell Signaling Cat#4499 Rabbit anti-PSD95 Cell Signaling Cat#3450 Goat anti-rabbit 680 LICOR Cat#926-32210 Goat anti-mouse 800 LICOR Cat#926-68071 Anti-mouse IgG, HRP-linked Cell Signaling Cat#7076 Anti-rabbit IgG, HRP-linked Cell Signaling Cat#7074 Rabbit anti-FLAG Cell Signaling Cat#14793 Mouse anti-HA Cell Signaling Cat#2367 Mouse anti-PSMA2 Thermo Fisher Scientific Cat#MA5-26149 Mouse anti PSMA1-3,5-7 Abcam Cat#MCP231 Rabbit anti PSMD11 Cell Signaling Cat#14303 Rabbit anti-CUL5 Atlas Antibodies Cat#HPA002185 Bacterial or virus strains DH5alpha Thermo Fisher Scientific Cat#18265017 BL21(DE3) Thermo Fisher Scientific Cat#EC0114 MegaX DH10B T1 R Thermo Fisher Scientific Cat#C640003 Chemicals, peptides, and recombinant proteins StemFlex Media Thermo Fisher Scientific Cat#A3349401 Y-27632 Stem Cell Technologies Cat#72308 Matrigel hESC-Qualified Matrix Corning Cat#354277 Doxycycline hyclate (Dox) Sigma-Aldrich Cat#D9891 Brain-derived neurotrophic factor (BDNF) PeproTech Cat#450-02 Neurotrophin-3 (NT-3) PeproTech Cat#450-03 (Continued on next page) ll OPEN ACCESS Cell 189, 1–18.e1–e10, March 5, 2026 e1 Please cite this article in press as: Samelson et al., CRISPR screens in iPSC-derived neurons reveal principles of tau proteostasis, Cell (2026), https://doi.org/10.1016/j.cell.2025.12.038 Article .. REAGENT or RESOURCE SOURCE IDENTIFIER Zymo Gigaprep kit Zymo Cat#D4204 Poly-D-Lysine Culture Dishes Corning Cat#354550 Universal Mycoplasma Detection Kit ATCC Cat#30-1012K Deposited data Raw and processed data (bulk CRISPR-screen data) This paper N/A Experimental models: Cell lines HEK293T ATCC Cat#CRL-11268 Human WTC11 iPSC with Ngn2 transgene and CRISPRi integration Tian et al. 20 N/A Human WTC11 iPSC with Ngn2 transgene and CRISPRa integration Tian et al. 63 N/A Human WTC11 iPSC with Ngn2 transgene and CRISPRi integration WT/V337M This paper N/A Human WTC11 iPSC with Ngn2 transgene integration V337M/V337M Gilbert et al. 77 N/A Oligonucleotides Primers for sequencing; see Table S1 This paper N/A Primers for qPCR; see Table S2 This paper N/A Recombinant DNA pMD2.G Didier Trono Addgene #12259 pMDLg/pRRE Didier Trono Addgene #12251 pRSV-REV Didier Trono Addgene #12253 pC13N-CLYBL-CAG-dCas9-BFP-KRAB Martin Kampmann Addgene #127968 pMK1334 Martin Kampmann Addgene #127965 pAJS1197 Cul5-3xFlag-T2A-mApple This paper N/A pAJS1203 GFP-3xFlag-T2A-mApple This paper N/A pAJS1205 GFP-Tau-1-50-T2A-mApple This paper N/A pAJS1206 GFP-Tau-40-90-T2A-mApple This paper N/A pAJS1207 GFP-Tau-80-130-T2A-mApple This paper N/A pAJS1208 GFP-Tau-120-170-T2A-mApple This paper N/A pAJS1209 GFP-Tau-160-210-T2A-mApple This paper N/A pAJS1210 GFP-Tau-200-250-T2A-mApple This paper N/A pAJS1211 GFP-Tau-240-290-T2A-mApple This paper N/A pAJS1212 GFP-Tau-280-352-T2A-mApple This paper N/A pAJS1172 GFP-Tau-Metless This paper N/A pAJS1184 GFP-Tau-T2A-mApple This paper N/A pAJS1154 GFP This paper N/A pAJS1149 GFP-Tau This paper N/A pZTC-13-R1 Jizhong Zou Addgene #62196 pZT-C13-L1 Jizhong Zou Addgene #62197 pAJS1224 pET-Duet EloBC Gift of John Gross, University of California, San Francisco Gift pAJS1228 His-Sumo-Tau-1-172 This paper N/A pAJS1229 His-Sumo-Tau-1-176 This paper N/A pIVM_26 Alessio Ciulli Addgene #204501 pAJS1200 SOCS4-3xFlag-T2A-mApple This paper N/A pAJS1216 GFP-Tau-80-130-IRES-mApple This paper N/A (Continued on next page) ll OPEN ACCESS Cell 189, 1–18.e1–e10, March 5, 2026 e3 Please cite this article in press as: Samelson et al., CRISPR screens in iPSC-derived neurons reveal principles of tau proteostasis, Cell (2026), https://doi.org/10.1016/j.cell.2025.12.038 Article .. Human iPSCs (in the male WTC11 background 79 ) were cultured in StemFlex Medium on BioLite Cell Culture Treated Dishes (Thermo Fisher Scientific; assorted Cat. No.) coated with Growth Factor Reduced, Phenol Red-Free, LDEV-Free Ma-trigel Basement Membrane Matrix (Corning; Cat. No. 356231) diluted 1:100 in Knockout DMEM (GIBCO/Thermo Fisher Scientific; Cat. No. 10829-018).

    Article Title: Multimodal CRISPR screens uncover DDX39B as a global repressor of A-to-I RNA editing.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit monoclonal anti-ADAR1 Cell Signaling Technology Cat#81284S; RRID:AB_3068597 Mouse monoclonal anti-DYKDDDDK (HRP) Absin Bioscience Cat#abs830014; RRID:AB_3683650 Rabbit monoclonal anti-HA Cell Signaling Technology Cat#3724; RRID:AB_1549585 Rabbit monoclonal anti-UAP56 (DDX39B) Abcam Cat#ab181061; RRID:AB_3683651 Mouse monoclonal anti-ADAR2 Santa Cruz Biotechnology Cat#sc-73409; RRID:AB_2289194 Mouse monoclonal anti-SC35 Abcam Cat#ab11826; RRID:AB_298608 Mouse monoclonal anti-dsRNA, clone rJ2 Millipore Cat#MABE1134; RRID:AB_2819101 Mouse monoclonal anti-dsRNA SCICONS Cat#10010200; RRID:AB_2651015 Mouse monoclonal anti-HDAg Kerafast Cat#EHD001; RRID:AB_3696045 Bacterial and virus strains HDV infectious clone ZZ173 This paper N/A Stbl3 chemically competent E. coli ALPALIFEBIO Cat#KTSMCC1200 Chemicals, peptides, and recombinant proteins DMEM Thermo Fisher Scientific Cat#C11995500BT DMEM/F12 Thermo Fisher Scientific Cat#C11330500BT Opti-MEM I Reduced Serum Medium GIBCO Cat#31985070 Polyethylenimine Linear (PEI) MW40000 Yeasen Cat#40816ES03 Lipofectamine RNAiMax Reagent Invitrogen Cat#13778150 Puromycin solution Yeasen Cat#60209ES50 RNase A Yeasen Cat#10406ES03 DAPI Beyotime Cat#C1006 Critical commercial assays MolPure® Cell RNA Kit Yeasen Cat#19231ES50 Chip A Single Cell Kit v2.1 MobiDrop Cat#S050100301 High Throughput Single-Cell 3′ Transcriptome Kit v2.1 MobiDrop Cat# S050200301 3′ Dual Index Kit MobiDrop Cat# S050300301 Easy II Protein Quantitative Kit TransGen Cat# DQ111 Deposited data Raw bulk RNA-seq and scRNA-seq data This paper GSA-Human: HRA011402 Processed data This paper Zenodo: https://doi.org/10.5281/zenodo. .. 15295990 Experimental models: cell lines HEK293T ATCC Cat#CRL-3216 CRISPRi- HEK293T This paper N/A CRISPRi- HEK293T-ADAR2 This paper N/A HepG2-NTCP This paper N/A Oligonucleotides See Tables S2 and S3 NA N/A Recombinant DNA CREDITS vector This paper N/A pLV2-UBC-mCherry-Hyg-CMV-ADAR2 This paper N/A pMK1334 Addgene Cat127965 (Continued on next page) 18 Cell Reports 44, 116009, July 22, 2025 .. HEK293T, HuH7, and SH-SY5Y cells were purchased from ATCC and cultured in Dulbecco’s Modified Eagle’s Medium and DMEM/ F12 (Gibco) supplemented with 10% fetal bovine serum (TransGen) and 1% penicillin and streptomycin (Aladdin). iPSC was cultured and differentiated into neurons as described previously.33,43 HepG2-NTCP cells were cultured in DMEM supplemented with 10% fetal bovine serum (Sigma) and 1% penicillin and streptomycin (Sigma) and 2.5 μg/mL puromycin (MedChemExpress).



    Similar Products

    cropseq guide puro vector  (Addgene inc)
    96
    Addgene inc cropseq guide puro vector
    Cropseq Guide Puro Vector, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cropseq+vector/CROPseq-Guide-Puro+(Plasmid+%2386708)/pmc12680425-86-7-9
    Average 96 stars, based on 1 article reviews
    cropseq guide puro vector - by Bioz Stars, 2026-10
    96/100 stars
    		  Buy from Supplier
    cropseq guide puro egfp vector  (Addgene inc)
    96
    Addgene inc cropseq guide puro egfp vector
    Cropseq Guide Puro Egfp Vector, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cropseq+vector/CROPseq-Guide-Puro+(Plasmid+%2386708)/bio_rxiv__2025__11__07__687183-172-6-10
    Average 96 stars, based on 1 article reviews
    cropseq guide puro egfp vector - by Bioz Stars, 2026-10
    96/100 stars
    		  Buy from Supplier
    crop seq car vectors  (Addgene inc)
    96
    Addgene inc crop seq car vectors
    Crop Seq Car Vectors, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cropseq+vector/CROPseq-Guide-Puro+(Plasmid+%2386708)/pmc12545207-236-1-9
    Average 96 stars, based on 1 article reviews
    crop seq car vectors - by Bioz Stars, 2026-10
    96/100 stars
    		  Buy from Supplier
    grna screening vector  (Addgene inc)
    96
    Addgene inc grna screening vector
    a , Overview of <t>gRNA</t> assignment for CRISPRi screen. b , Coverage for each gRNA in CRISPRi screen. Data points represent means ± SEM. n = individual gRNAs from 1 SDR-seq experiment. c , Relative distance of gRNA binding site to transcription start site (TSS). Positive values are after TSS (within transcript), negative values before transcript. Size indicates P -value calculated using MAST with Benjamini-Hochberg correction for multiple testing. Significant hits ( P -value < 0.05) are colored. d , Outline of testing for PE iPSCs. Editing can be measured by repairing a <t>non-functional</t> <t>EGFP</t> that was integrated via a lentivirus. e , Flow cytometry indicating editing in PE iPSCs.
    Grna Screening Vector, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cropseq+vector/CROPseq-Guide-Puro+(Plasmid+%2386708)/pmc12510883-351-1-9
    Average 96 stars, based on 1 article reviews
    grna screening vector - by Bioz Stars, 2026-10
    96/100 stars
    		  Buy from Supplier
    crop seq v2 vector  (Addgene inc)
    93
    Addgene inc crop seq v2 vector
    a , Overview of <t>gRNA</t> assignment for CRISPRi screen. b , Coverage for each gRNA in CRISPRi screen. Data points represent means ± SEM. n = individual gRNAs from 1 SDR-seq experiment. c , Relative distance of gRNA binding site to transcription start site (TSS). Positive values are after TSS (within transcript), negative values before transcript. Size indicates P -value calculated using MAST with Benjamini-Hochberg correction for multiple testing. Significant hits ( P -value < 0.05) are colored. d , Outline of testing for PE iPSCs. Editing can be measured by repairing a <t>non-functional</t> <t>EGFP</t> that was integrated via a lentivirus. e , Flow cytometry indicating editing in PE iPSCs.
    Crop Seq V2 Vector, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cropseq+vector/CROPseq-puro-v2+(Plasmid+%23127458)/bio_rxiv__2025__03__17__643814-235-23-26
    Average 93 stars, based on 1 article reviews
    crop seq v2 vector - by Bioz Stars, 2026-10
    93/100 stars
    		  Buy from Supplier
    crop seq guide puro vector  (Addgene inc)
    96
    Addgene inc crop seq guide puro vector
    a , Overview of <t>gRNA</t> assignment for CRISPRi screen. b , Coverage for each gRNA in CRISPRi screen. Data points represent means ± SEM. n = individual gRNAs from 1 SDR-seq experiment. c , Relative distance of gRNA binding site to transcription start site (TSS). Positive values are after TSS (within transcript), negative values before transcript. Size indicates P -value calculated using MAST with Benjamini-Hochberg correction for multiple testing. Significant hits ( P -value < 0.05) are colored. d , Outline of testing for PE iPSCs. Editing can be measured by repairing a <t>non-functional</t> <t>EGFP</t> that was integrated via a lentivirus. e , Flow cytometry indicating editing in PE iPSCs.
    Crop Seq Guide Puro Vector, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cropseq+vector/CROPseq-Guide-Puro+(Plasmid+%2386708)/bio_rxiv__2025__03__17__643322-241-7-9
    Average 96 stars, based on 1 article reviews
    crop seq guide puro vector - by Bioz Stars, 2026-10
    96/100 stars
    		  Buy from Supplier

    Image Search Results


    a , Overview of gRNA assignment for CRISPRi screen. b , Coverage for each gRNA in CRISPRi screen. Data points represent means ± SEM. n = individual gRNAs from 1 SDR-seq experiment. c , Relative distance of gRNA binding site to transcription start site (TSS). Positive values are after TSS (within transcript), negative values before transcript. Size indicates P -value calculated using MAST with Benjamini-Hochberg correction for multiple testing. Significant hits ( P -value < 0.05) are colored. d , Outline of testing for PE iPSCs. Editing can be measured by repairing a non-functional EGFP that was integrated via a lentivirus. e , Flow cytometry indicating editing in PE iPSCs.

    Journal: Nature Methods

    Article Title: Functional phenotyping of genomic variants using joint multiomic single-cell DNA–RNA sequencing

    doi: 10.1038/s41592-025-02805-0

    Figure Lengend Snippet: a , Overview of gRNA assignment for CRISPRi screen. b , Coverage for each gRNA in CRISPRi screen. Data points represent means ± SEM. n = individual gRNAs from 1 SDR-seq experiment. c , Relative distance of gRNA binding site to transcription start site (TSS). Positive values are after TSS (within transcript), negative values before transcript. Size indicates P -value calculated using MAST with Benjamini-Hochberg correction for multiple testing. Significant hits ( P -value < 0.05) are colored. d , Outline of testing for PE iPSCs. Editing can be measured by repairing a non-functional EGFP that was integrated via a lentivirus. e , Flow cytometry indicating editing in PE iPSCs.

    Article Snippet: The gRNA screening vector was a modified CROP-seq vector (Addgene, 86708) to also express eGFP and include a distinct gRNA CS in the scaffold of the gRNA .

    Techniques: Binding Assay, Functional Assay, Flow Cytometry