fcs2 live cell chamber (Bioptechs inc)

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Bioptechs inc fcs2 live cell chamber
Fcs2 Live Cell Chamber, supplied by Bioptechs inc, used in various techniques. Bioz Stars score: 96/100, based on 445 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/coverslip/Cooled+FCS2/pm09166399-154-7-11
Average 96 stars, based on 445 article reviews

fcs2 live cell chamber - by Bioz Stars, 2026-10

96/100 stars

Images

Microscopy:

Article Title: CD49d governs immune synapse formation through actin rearrangements and synchronizes BCR signaling in CLL.
Article Snippet: .. 179 Real-time microscopy on planar lipid bilayers 180 Artificial planar lipid bilayers were assembled in FCS2 chambers (Bioptechs) as 181 previously described.16 TCL1-tg and CD49dΔB TCL1-tg leukemic cells (4x106) were 182 purified from total splenocytes by negative selection with EasySepTM Mouse Pan-B 183 cells Isolation kit (StemCell Technologies), and co-injected (4x106) into the warmed 184 chamber (37oC) for imaging. ..

Purification:

Selection:

Isolation:

Imaging:

Article Title: HaloTag-based approach to quantify subcellular localization of TRPV3 channels.
Article Snippet: All analysis was carried out using Igor Pro 9 (Wavemetrics) or Microsoft Excel. .. Recordings were performed using a temperature-controlled Delta T imaging chamber (Bioptechs). ..

Fluorescence:

Article Title: Molecular mechanism of p-Coumaric acid in the regulation of AQP2 function through the activation of calcium-sensing receptor signaling.
Article Snippet: .. In fluorescence measurements, the coverslips with dye-loaded cells were mounted in a perfusion chamber (FCS2 Closed Chamber System, Bioptechs, Butler, U.S.A.). .. Measurements were carried out using an inverted TE2000-S microscope (Nikon Eclipse Microscope, Tokyo, Japan).

Inverted Microscopy:

Article Title: Motility Functions as an Essential Defense in the Bacterial Persister Lifecycle
Article Snippet: .. To capture the natural swimming behavior of cells in their original living environment, we directly placed 8 μL of bacterial culture onto the Bioptechs FCS2 chamber (0.2 mm plastic pad between slide and coverslip) and used a Nikon ECLIPSE Ti inverted microscope to record bright-field videos at approximately 22 frames per second (FPS). ..

Staining:

Article Title: Linker compounds
Article Snippet: Readout probes are DNA oligonucleotides with a sequence complementary to the oligonucleotides conjugated to each antibody, modified at the 5′ end with a fluorescent group (Atto 565 or Cy5) conjugated via a disulphide cleavable linker (obtained from Biomers GmBh, Germany) ProFISH Imaging Cyclic imaging was done on a modified Leica Dmi8 microscope equipped with a high-power light engine (Omicron BriXXHub) with lasers lines at 488 nm, 561 nm and 647 nm. .. A custom fluidic system was used to automate the readout staining cycles used to detect each antibody, based on a Bioptechs FCS2 chamber connected to a Gilson Minipuls 3 pump and to a series of valves (Hamilton MVP) controlled by a custom python script. .. Fixed samples were embedded in 4% oxydised agarose and re-embedded in a proprietary monomer solution produced by Tissuevision Inc (Newton, MA, USA), before sectioning and imaging on a Tissuecyte 1000 Serial two-photon tomography system.

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