open porous structured core-shell microtissue (BioMimetic Therapeutics)
90
Structured Review
BioMimetic Therapeutics
open porous structured core-shell microtissue
Open Porous Structured Core Shell Microtissue, supplied by BioMimetic Therapeutics, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/core+shell+structure/open+porous+structured+core+shell+microtissue/pmc06643438-32-7-6
Average 90 stars, based on 1 article reviews
Open Porous Structured Core Shell Microtissue, supplied by BioMimetic Therapeutics, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/core+shell+structure/open+porous+structured+core+shell+microtissue/pmc06643438-32-7-6
Average 90 stars, based on 1 article reviews
open porous structured core-shell microtissue - by Bioz Stars,
2026-11
90/100 stars
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Article Title: Biomimetic open porous structured core-shell microtissue with enhanced mechanical properties for bottom-up bone tissue engineering
Journal: Theranostics
doi: 10.7150/thno.34464
Figure Legend Snippet: Diagram of experimental design. Briefly, 10 mg/mL DBM particles were added into 60 mg/mL gelatin solution. The mixture was then ultrasonically dispersed until DBM particles were uniformly distributed. EDC/NHS were added to crosslink the precursor solution, and the crosslinked precursor solution was then pipetted into micro-stencil array chip, after which the lyophilized core-shell micro-scaffolds in the chip were harvested using an Ejector Pin array. BMSCs were seeded onto core-shell micro-scaffolds to form core-shell microtissue which was composed of DBM core loaded with BMP-2 and a gelatin shell enclosing the core. After the dynamic expansion and osteo-differentiation period in the perfusion bioreactor, the core-shell microtissues assembled together to form TEBGs. The assembled TEBGs were then implanted into rat critical sized cranial defect site to evaluate its ability to repair the defect.
Techniques Used:
Figure Legend Snippet: H&E and immunohistochemical staining of CD31 in the subcutaneous model of different groups after 4 weeks implantation. (A, B) H&E staining found that core-shell microtissue has the most amount of new bone tissue compared to other groups (White arrows indicate remained DBM particles, black arrows indicate new bone tissue). (C) Immunohistostaining of CD31 showed that core-shell microtissues formed the most amount of newly capillaries (Red arrows indicate newly formed vessels). (D) The mean vessel numbers quantified using Image-Pro Plus 6.0 software. (*: p < 0.05, **: p < 0.01, scale bars in A: 500 μm, scale bars in B: 100 μm, scale bars in C: 25 μm)
Techniques Used: Immunohistochemical staining, Staining, Software
Figure Legend Snippet: Micro-CT analysis of TEBGs for rat critical sized bone defect treatment. (A-D) 3D images of defect sites treated by core-shell microtissue/core-shell micro-scaffold/gelatin microtissue/gelatin micro-scaffold based TEBGs were reconstructed at 4 weeks and 12 weeks post treatment, respectively. (The black dotted circles indicate 5 mm critical sized bone defect). (E-G) Quantitative analyses of BV, BV/TV ratio and BMD at week 4 and week 12. (*: p < 0.05, ***: p < 0.001)
Techniques Used: Micro-CT
Figure Legend Snippet: H&E, Masson's Trichrome staining and immuno-histochemical staining of col-1 and OCN of the defect sites after 12 weeks implantation. (A-D) Core-shell microtissue explant had a dense new bone tissue and the most calcium nodules in the defect site (Black arrows indicate the boarders of defect sites). (E, F) Immunohistochemical staining of col-1 and OCN (White arrows indicate the stained type 1 collagen, red arrows indicate OCN-positive cells). (Scale bars in A, C: 500 μm, scale bars in B: 100 μm, scale bars in D: 200 μm, scale bars in E, F: 50 μm)
Techniques Used: Staining, Immunohistochemical staining
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