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control igg  (Proteintech)


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    Structured Review

    Proteintech control igg
    Control Igg, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 1294 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/controller/IgG+control+Antibody/pmc13019968-295-3-5
    Average 96 stars, based on 1294 article reviews
    control igg - by Bioz Stars, 2026-10
    96/100 stars

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    Related Articles

    Incubation:

    Article Title: PREX1 regulates renal fibrosis through the RPTOR-mTORC1 signaling pathway.
    Article Snippet: For Co-IP assays, HK-2 cells were lysed in NP-40 buffer (Beyotime) containing protease and phosphatase inhibitors. .. Cell lysates containing 1 mg of total protein were incubated overnight at 4 ◦C with 5 μg of antiPREX1 antibody (Proteintech, Cat# 22460–1-AP) or control IgG (Proteintech, Cat# 30000–0-AP), followed by incubation with Protein A/G magnetic beads (MedChemExpress, Cat# HY-K0205) for 2 h at 4 ◦C. ..

    Article Title: Cryopreservation Disrupts TRIM21-Mediated AKAP3 Ubiquitination and Triggers Stress Granule Formation in Human Sperm.
    Article Snippet: .. One was incubated with 10 μg Rabbit IgG control Polyclonal antibody (30000-0-AP, Proteintech), and the other was incubated with 10 μg rabbit antiFlag monoclonal antibody (80801-2-RR, Proteintech) overnight at 4◦C inversion. .. The next day, the prewashed Pierce Protein A/G Magnetic Beads (88802, Thermo Scientific) were added to the mixture according to the manufacturer’s protocol.

    Article Title: The ribonuclease ZC3H12A stabilizes BRCA1 mRNA in DNA damage response.
    Article Snippet: .. The remaining lysate was divided equally; one half was incubated with 5 μg of anti-ZC3H12A antibody (Proteintech) and BeyoMag Protein A + G beads to precipitate ZC3H12A, while the other half was incubated with 5 μg of rabbit IgG (Proteintech) as a negative control. ..

    Article Title: Akkermansia muciniphila protects diabetes-associated cognitive decline by enhancing leucine catabolism and suppressing neuronal excessive mitophagy.
    Article Snippet: .. After centrifugation, the protein extracts were incubated overnight at 4 °C with 1 μg of anti-OPTN antibody (60293-1-Ig, Proteintech) or control IgG under gentle agitation. .. Subsequently, 50 μL of protein A/G agarose beads (P2055, Beyotime Technology, Shanghai, China) was added, and the mixtures were further incubated at 4 °C for 3 h. The samples were then centrifuged at 1000 × g for 3 min, and the supernatants were discarded to collect the immunocomplexes.

    Control:

    Article Title: PREX1 regulates renal fibrosis through the RPTOR-mTORC1 signaling pathway.
    Article Snippet: For Co-IP assays, HK-2 cells were lysed in NP-40 buffer (Beyotime) containing protease and phosphatase inhibitors. .. Cell lysates containing 1 mg of total protein were incubated overnight at 4 ◦C with 5 μg of antiPREX1 antibody (Proteintech, Cat# 22460–1-AP) or control IgG (Proteintech, Cat# 30000–0-AP), followed by incubation with Protein A/G magnetic beads (MedChemExpress, Cat# HY-K0205) for 2 h at 4 ◦C. ..

    Article Title: Cryopreservation Disrupts TRIM21-Mediated AKAP3 Ubiquitination and Triggers Stress Granule Formation in Human Sperm.
    Article Snippet: .. One was incubated with 10 μg Rabbit IgG control Polyclonal antibody (30000-0-AP, Proteintech), and the other was incubated with 10 μg rabbit antiFlag monoclonal antibody (80801-2-RR, Proteintech) overnight at 4◦C inversion. .. The next day, the prewashed Pierce Protein A/G Magnetic Beads (88802, Thermo Scientific) were added to the mixture according to the manufacturer’s protocol.

    Article Title: Magnolol enhances yak oocyte maturation and blastocyst quality by improving lipid metabolism and redox homeostasis through a PPARγ-dependent mechanism.
    Article Snippet: Nuclei were counterstained with 4′,6-Diamidino-2-phenylindole (DAPI, SL7101, Coolaber, China). .. Rabbit IgG Isotype Control (1:100; #98136-1-RR, Proteintech) and Mouse IgG Isotype Control (1:100; #HY-P80757, MCE) were used as negative controls in place of the primary antibody, and no specific fluorescence signal was detected (Supplementary Material 1). .. Images were acquired using a DeltaVision Ultra imaging system (GE Healthcare Bio-Sciences Corp., USA) equipped with a 20 × objective.

    Article Title: Targeting the DNHD1‑HDAC6 axis inactivates prostate cancer cell aggressiveness in vitro , suggesting potential therapeutic vulnerability.
    Article Snippet: .. Cell lysates (100 μg per reaction) were immunoprecipitated with anti‐E2F6 (2 μg/reaction; cat. no. 31464‐1‐AP; Proteintech Group, Inc.) or control IgG (2 μg/reaction; cat. no. 30000‐0‐AP; Proteintech Group, Inc.) overnight (12‐16 h) at 4 ̊C with magnetic A/G beads. ..

    Article Title: CD38-activated macrophages drive age-related placental senescence by depleting NAD + in decidual stromal cells.
    Article Snippet: .. Chromatin extracts containing DNA fragments with an average size of 500bp were immunoprecipitated overnight at 4 °Cusing an anti-IRF5 antibody (Cat. No. 11312-1-AP, Proteintech), or IgG control (included in the kit). ..

    Article Title: Akkermansia muciniphila protects diabetes-associated cognitive decline by enhancing leucine catabolism and suppressing neuronal excessive mitophagy.
    Article Snippet: .. After centrifugation, the protein extracts were incubated overnight at 4 °C with 1 μg of anti-OPTN antibody (60293-1-Ig, Proteintech) or control IgG under gentle agitation. .. Subsequently, 50 μL of protein A/G agarose beads (P2055, Beyotime Technology, Shanghai, China) was added, and the mixtures were further incubated at 4 °C for 3 h. The samples were then centrifuged at 1000 × g for 3 min, and the supernatants were discarded to collect the immunocomplexes.

    Article Title: piRNA-mediated silencing of exogenous retrovirus in mouse spermatogonial stem cells.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Mouse anti-ACTB SIGMA Cat# A5441; RRID: AB_476744 Rabbit anti-DNMT3A(E9P2F) Cell Signal Cat# 49768; RRID: AB_2799365 Mouse anti-Dnmt3b(G-9) Santa Cruz Cat# sc-376043; RRID: AB_10988201 GFP Polyclonal Antibody invitrogen Cat# A-11122; RRID: AB_221569 Mouse anti-GLP/EHMT1 R&D SYSTEMS Cat# PP-B0422-00; RRID: AB_2097494 Rabbit anti-G9a/EHMT2 Cell Signal Cat# 3306S; RRID: AB_2097647 Goat anti-GFRA1 R&D SYSTEMS Cat# AF560; RRID: AB_2110307 Mouse anti-trimethyl-Histone H3 (Lys9) Millipore Cat# 07-442; RRID: AB_310620 Mouse anti-dimethyl-Histone H3 (Lys9) Millipore Cat# 07-441; RRID: AB_11212297 Mouse anti-MAGE-A(6C1) Santa Cruz Cat# sc-20034; RRID: AB_627907 Rabbit anti-MPP8 (MPHOSPH8) proteintech Cat# 16796-1-AP; RRID: AB_2266644 Rabbit anti-PIWIL2 abcam Cat# ab36764; RRID: AB_777284 Rabbit anti-PIWIL1-C Kuramochi-Miyagawa et al.26 N/A Rabbit anti-SETDB1 proteintech Cat# 11231-1-AP; RRID: AB_2186069 Rabbit anti-SYCP3 Novus Bio Cat# NB300-231; RRID: AB_10002746 Rabbit anti-TRP53 Novus Bio Cat# NBP2-62555; RRID: AB_3352998 Rabbit IgG control proteintech Cat# 30000-0-AP; RRID: AB_2819035 Normal mouse IgG Santa Cruz Cat# sc-2025; RRID: AB_737182 Alexa Fluor 488 donkey anti-rabbit IgG (H + L) Molecular Probes Cat# A21206; RRID: AB_2535792 Alexa Fluor 555 donkey anti-rabbit IgG (H + L) Molecular Probes Cat# A31572; RRID: AB_162543 Alexa Fluor 555 anti-goat IgG Molecular Probes Cat# A21432; RRID: AB_141788 Anti-rabbit IgG, HRP-linked antibody Cell signal Cat# 7074; RRID: AB_2099233 Anti-mouse IgG, HRP-linked antibody Cell signal Cat# 7076; RRID: AB_330924 Donkey anti-Goat IgG H&L (HRP) abcam Cat# ab97110; RRID: AB_10679463 Mouse TrueBlot ULTRA Anti-Mouse Ig HRP ROCLAND Cat# 18-8817-30; RRID: AB_2610849 Bacterial and virus strains shControl Addgene #1864 shPiwil2 Horizon Discovery TRCN0000009646 TRCN0000009647 TRCN0000009648 TRCN0000011812 TRCN0000011811 shPiwil1 Horizon Discovery TRCN0000009642 TRCN0000009643 TRCN0000009644 TRCN0000009645 shTrp53 Horizon Discovery TRCN0000012358 TRCN0000012359 TRCN0000012360 TRCN0000012361 TRCN0000012362 shMagea Horizon Discovery TRCN0000112760 TRCN0000112761 TRCN0000112762 TRCN0000112763 TRCN0000112764 (Continued on next page) Cell Reports 45, 117992, October 27, 2026 17 .. REAGENT or RESOURCE SOURCE IDENTIFIER shTrim28 Horizon Discovery TRCN0000071363 TRCN0000071364 TRCN0000071365 TRCN0000071366 TRCN0000071367 shMphosph8 Horizon Discovery TRCN0000085438 TRCN0000085439 TRCN0000085440 TRCN0000085441 TRCN0000085442 shSetdb1 Horizon Discovery TRCN0000092973 TRCN0000092974 TRCN0000092975 TRCN0000092976 TRCN0000092977 shEhmt1 Horizon Discovery TRCN0000086068 TRCN0000086070 TRCN0000086071 TRCN0000086072 shEhmt2 Horizon Discovery TRCN0000054543 TRCN0000054544 TRCN0000054546 TRCN0000054547 pLV-CMV-Egfp In(WT) Dr. M. Ikawa (Osaka University) N/A pLV-CMV-Egfp In(D64V) Dr. M. Ikawa (Osaka University), Certo et al.36 N/A CSII-Piwil2-WT This study N/A CSII-Piwil2-D813A This study N/A CSII-Trp53 This study N/A CSII-Magea5 This study N/A CSII-Dnmt3a1 This study N/A CSII-Dnmt3a2 This study N/A pLKO-Control sponge This study N/A pLKO-miR-224 sponge This study N/A pLKO-miR-543 sponge This study N/A pLKO-miR-224 mimic This study N/A pLKO-miR-543 mimic This study N/A Deposited data Small RNA-seq This study GSE299335 RNA-seq This study GSE242728 Experimental models: Cell lines WT GSC Kanatsu-Shinohara et al.34 N/A Trp53-KO GSC Kanatsu-Shinohara et al.69 N/A Piwil2-KO GSC Shiromoto et al.39 AES0197 Piwil4-KO GSC Kuramochi-Miyagawa et al.40 N/A Dnmt3a/3b floxed GSC Takashima et al.46 N/A Experimental models: Organisms/strains DBA/2 mouse (M. musculus) Japan SLC DBA/2CrSlc WBB6F1-W/Wv (W) mice (M. musculus) Japan SLC WBB6F1/Kit-KitW/KitW-v/Slc Piwil2-KO mouse (M. musculus) Dr. T. Nakano (Osaka University, Osaka, Japan) RBRC01361 Trp53-KO mouse (M. musculus) RIKEN BRC – (Continued on next page) 18 Cell Reports 45, 117992, October 27, 2026

    Magnetic Beads:

    Article Title: PREX1 regulates renal fibrosis through the RPTOR-mTORC1 signaling pathway.
    Article Snippet: For Co-IP assays, HK-2 cells were lysed in NP-40 buffer (Beyotime) containing protease and phosphatase inhibitors. .. Cell lysates containing 1 mg of total protein were incubated overnight at 4 ◦C with 5 μg of antiPREX1 antibody (Proteintech, Cat# 22460–1-AP) or control IgG (Proteintech, Cat# 30000–0-AP), followed by incubation with Protein A/G magnetic beads (MedChemExpress, Cat# HY-K0205) for 2 h at 4 ◦C. ..

    Fluorescence:

    Article Title: Magnolol enhances yak oocyte maturation and blastocyst quality by improving lipid metabolism and redox homeostasis through a PPARγ-dependent mechanism.
    Article Snippet: Nuclei were counterstained with 4′,6-Diamidino-2-phenylindole (DAPI, SL7101, Coolaber, China). .. Rabbit IgG Isotype Control (1:100; #98136-1-RR, Proteintech) and Mouse IgG Isotype Control (1:100; #HY-P80757, MCE) were used as negative controls in place of the primary antibody, and no specific fluorescence signal was detected (Supplementary Material 1). .. Images were acquired using a DeltaVision Ultra imaging system (GE Healthcare Bio-Sciences Corp., USA) equipped with a 20 × objective.

    Immunoprecipitation:

    Article Title: Targeting the DNHD1‑HDAC6 axis inactivates prostate cancer cell aggressiveness in vitro , suggesting potential therapeutic vulnerability.
    Article Snippet: .. Cell lysates (100 μg per reaction) were immunoprecipitated with anti‐E2F6 (2 μg/reaction; cat. no. 31464‐1‐AP; Proteintech Group, Inc.) or control IgG (2 μg/reaction; cat. no. 30000‐0‐AP; Proteintech Group, Inc.) overnight (12‐16 h) at 4 ̊C with magnetic A/G beads. ..

    Article Title: CD38-activated macrophages drive age-related placental senescence by depleting NAD + in decidual stromal cells.
    Article Snippet: .. Chromatin extracts containing DNA fragments with an average size of 500bp were immunoprecipitated overnight at 4 °Cusing an anti-IRF5 antibody (Cat. No. 11312-1-AP, Proteintech), or IgG control (included in the kit). ..

    Negative Control:

    Article Title: The ribonuclease ZC3H12A stabilizes BRCA1 mRNA in DNA damage response.
    Article Snippet: .. The remaining lysate was divided equally; one half was incubated with 5 μg of anti-ZC3H12A antibody (Proteintech) and BeyoMag Protein A + G beads to precipitate ZC3H12A, while the other half was incubated with 5 μg of rabbit IgG (Proteintech) as a negative control. ..

    Centrifugation:

    Article Title: Akkermansia muciniphila protects diabetes-associated cognitive decline by enhancing leucine catabolism and suppressing neuronal excessive mitophagy.
    Article Snippet: .. After centrifugation, the protein extracts were incubated overnight at 4 °C with 1 μg of anti-OPTN antibody (60293-1-Ig, Proteintech) or control IgG under gentle agitation. .. Subsequently, 50 μL of protein A/G agarose beads (P2055, Beyotime Technology, Shanghai, China) was added, and the mixtures were further incubated at 4 °C for 3 h. The samples were then centrifuged at 1000 × g for 3 min, and the supernatants were discarded to collect the immunocomplexes.

    Gentle:

    Article Title: Akkermansia muciniphila protects diabetes-associated cognitive decline by enhancing leucine catabolism and suppressing neuronal excessive mitophagy.
    Article Snippet: .. After centrifugation, the protein extracts were incubated overnight at 4 °C with 1 μg of anti-OPTN antibody (60293-1-Ig, Proteintech) or control IgG under gentle agitation. .. Subsequently, 50 μL of protein A/G agarose beads (P2055, Beyotime Technology, Shanghai, China) was added, and the mixtures were further incubated at 4 °C for 3 h. The samples were then centrifuged at 1000 × g for 3 min, and the supernatants were discarded to collect the immunocomplexes.

    Virus:

    Article Title: piRNA-mediated silencing of exogenous retrovirus in mouse spermatogonial stem cells.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Mouse anti-ACTB SIGMA Cat# A5441; RRID: AB_476744 Rabbit anti-DNMT3A(E9P2F) Cell Signal Cat# 49768; RRID: AB_2799365 Mouse anti-Dnmt3b(G-9) Santa Cruz Cat# sc-376043; RRID: AB_10988201 GFP Polyclonal Antibody invitrogen Cat# A-11122; RRID: AB_221569 Mouse anti-GLP/EHMT1 R&D SYSTEMS Cat# PP-B0422-00; RRID: AB_2097494 Rabbit anti-G9a/EHMT2 Cell Signal Cat# 3306S; RRID: AB_2097647 Goat anti-GFRA1 R&D SYSTEMS Cat# AF560; RRID: AB_2110307 Mouse anti-trimethyl-Histone H3 (Lys9) Millipore Cat# 07-442; RRID: AB_310620 Mouse anti-dimethyl-Histone H3 (Lys9) Millipore Cat# 07-441; RRID: AB_11212297 Mouse anti-MAGE-A(6C1) Santa Cruz Cat# sc-20034; RRID: AB_627907 Rabbit anti-MPP8 (MPHOSPH8) proteintech Cat# 16796-1-AP; RRID: AB_2266644 Rabbit anti-PIWIL2 abcam Cat# ab36764; RRID: AB_777284 Rabbit anti-PIWIL1-C Kuramochi-Miyagawa et al.26 N/A Rabbit anti-SETDB1 proteintech Cat# 11231-1-AP; RRID: AB_2186069 Rabbit anti-SYCP3 Novus Bio Cat# NB300-231; RRID: AB_10002746 Rabbit anti-TRP53 Novus Bio Cat# NBP2-62555; RRID: AB_3352998 Rabbit IgG control proteintech Cat# 30000-0-AP; RRID: AB_2819035 Normal mouse IgG Santa Cruz Cat# sc-2025; RRID: AB_737182 Alexa Fluor 488 donkey anti-rabbit IgG (H + L) Molecular Probes Cat# A21206; RRID: AB_2535792 Alexa Fluor 555 donkey anti-rabbit IgG (H + L) Molecular Probes Cat# A31572; RRID: AB_162543 Alexa Fluor 555 anti-goat IgG Molecular Probes Cat# A21432; RRID: AB_141788 Anti-rabbit IgG, HRP-linked antibody Cell signal Cat# 7074; RRID: AB_2099233 Anti-mouse IgG, HRP-linked antibody Cell signal Cat# 7076; RRID: AB_330924 Donkey anti-Goat IgG H&L (HRP) abcam Cat# ab97110; RRID: AB_10679463 Mouse TrueBlot ULTRA Anti-Mouse Ig HRP ROCLAND Cat# 18-8817-30; RRID: AB_2610849 Bacterial and virus strains shControl Addgene #1864 shPiwil2 Horizon Discovery TRCN0000009646 TRCN0000009647 TRCN0000009648 TRCN0000011812 TRCN0000011811 shPiwil1 Horizon Discovery TRCN0000009642 TRCN0000009643 TRCN0000009644 TRCN0000009645 shTrp53 Horizon Discovery TRCN0000012358 TRCN0000012359 TRCN0000012360 TRCN0000012361 TRCN0000012362 shMagea Horizon Discovery TRCN0000112760 TRCN0000112761 TRCN0000112762 TRCN0000112763 TRCN0000112764 (Continued on next page) Cell Reports 45, 117992, October 27, 2026 17 .. REAGENT or RESOURCE SOURCE IDENTIFIER shTrim28 Horizon Discovery TRCN0000071363 TRCN0000071364 TRCN0000071365 TRCN0000071366 TRCN0000071367 shMphosph8 Horizon Discovery TRCN0000085438 TRCN0000085439 TRCN0000085440 TRCN0000085441 TRCN0000085442 shSetdb1 Horizon Discovery TRCN0000092973 TRCN0000092974 TRCN0000092975 TRCN0000092976 TRCN0000092977 shEhmt1 Horizon Discovery TRCN0000086068 TRCN0000086070 TRCN0000086071 TRCN0000086072 shEhmt2 Horizon Discovery TRCN0000054543 TRCN0000054544 TRCN0000054546 TRCN0000054547 pLV-CMV-Egfp In(WT) Dr. M. Ikawa (Osaka University) N/A pLV-CMV-Egfp In(D64V) Dr. M. Ikawa (Osaka University), Certo et al.36 N/A CSII-Piwil2-WT This study N/A CSII-Piwil2-D813A This study N/A CSII-Trp53 This study N/A CSII-Magea5 This study N/A CSII-Dnmt3a1 This study N/A CSII-Dnmt3a2 This study N/A pLKO-Control sponge This study N/A pLKO-miR-224 sponge This study N/A pLKO-miR-543 sponge This study N/A pLKO-miR-224 mimic This study N/A pLKO-miR-543 mimic This study N/A Deposited data Small RNA-seq This study GSE299335 RNA-seq This study GSE242728 Experimental models: Cell lines WT GSC Kanatsu-Shinohara et al.34 N/A Trp53-KO GSC Kanatsu-Shinohara et al.69 N/A Piwil2-KO GSC Shiromoto et al.39 AES0197 Piwil4-KO GSC Kuramochi-Miyagawa et al.40 N/A Dnmt3a/3b floxed GSC Takashima et al.46 N/A Experimental models: Organisms/strains DBA/2 mouse (M. musculus) Japan SLC DBA/2CrSlc WBB6F1-W/Wv (W) mice (M. musculus) Japan SLC WBB6F1/Kit-KitW/KitW-v/Slc Piwil2-KO mouse (M. musculus) Dr. T. Nakano (Osaka University, Osaka, Japan) RBRC01361 Trp53-KO mouse (M. musculus) RIKEN BRC – (Continued on next page) 18 Cell Reports 45, 117992, October 27, 2026



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