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control replicate  (Cell Signaling Technology Inc)


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    Structured Review

    Cell Signaling Technology Inc control replicate
    Control Replicate, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 99/100, based on 5349 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/control+replicate/Normal+Rabbit+IgG/ppr0851166-212-33-41
    Average 99 stars, based on 5349 article reviews
    control replicate - by Bioz Stars, 2026-11
    99/100 stars

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    Related Articles

    Incubation:

    Article Title: HBV integration into KMT2B increases malignant phenotype of hepatocellular carcinoma by epigenetic reprogramming.
    Article Snippet: .. Cell lysates were incubated with anti-HA antibody (#3724, CST, 1:50) or normal rabbit IgG (CST) overnight at 4 ◦C with rotation. .. Protein A/G magnetic beads (Thermo Fisher, #88804) were added and incubated for an additional 2 h at 4 ◦C.

    Article Title: ESAM reduces sensitivity to anti-HER2 therapy in HER2-positive breast cancer by activating the mTOR pathway
    Article Snippet: .. Equal amounts of total protein were incubated with an anti-Calnexin antibody (5 μg, HUABIO, Cat. No. ER1803-42) or the corresponding normal rabbit IgG control (5 μg, CST, Cat. No. 2729), followed by incubation with Protein A/G beads. .. The immunoprecipitates were washed and analyzed by immunoblotting using an anti-ubiquitin antibody (1:500, CST, Cat. No. 3936) and an anti-Calnexin antibody (1:1000, CST, Cat. No. 2679).

    Article Title: GTPase GBP7 promotes HSV-1 infection by interacting with and activating eEF1A1 to accelerate the protein translation process.
    Article Snippet: After incubation for 24 h, the cells were lysed with RIPA lysis buffer (Beyotime, Cat# P0013) and subsequently centrifuged at 12,000 rpm and 4°C for 15 min. .. The supernatant was incubated overnight at 4°C with Proteo G Resin (GenScript, Cat# L00209-50), which had been preincubated with either GBP7-Flag or IgG antibodies (CST, Cat# 2729S). ..

    Article Title: Circ_0000128 increases the progression of NSCLC through the miR-654-5p/OTUB1/Wnt pathway axis.
    Article Snippet: .. The lysis buffer was incubated with anti-AGO2 antibody (Cell Signalling Technology 2897) or isogenic IgG (Cell Signalling Technology 2729), after which protein A/G magnetic beads were added. ..

    Control:

    Article Title: ESAM reduces sensitivity to anti-HER2 therapy in HER2-positive breast cancer by activating the mTOR pathway
    Article Snippet: .. Equal amounts of total protein were incubated with an anti-Calnexin antibody (5 μg, HUABIO, Cat. No. ER1803-42) or the corresponding normal rabbit IgG control (5 μg, CST, Cat. No. 2729), followed by incubation with Protein A/G beads. .. The immunoprecipitates were washed and analyzed by immunoblotting using an anti-ubiquitin antibody (1:500, CST, Cat. No. 3936) and an anti-Calnexin antibody (1:1000, CST, Cat. No. 2679).

    Article Title: YY1-NSUN7-m 5 C-HSPA1B axis drives polycystic ovary syndrome pathogenesis via epigenetic stabilization of HSPA1B mRNA.
    Article Snippet: Polycystic Ovary Syndrome (PCOS) is the most common endocrine disorder in reproductive-age women.. Heat shock 70 kDa protein 1B (HSPA1B) is critical for stress response and inflammation, but its role in PCOS remains unknown.. To validate HSPA1B as a key pathogenic gene in PCOS and investigate its regulatory mechanisms.

    Immunoprecipitation:

    Article Title: KDM6 Histone Demethylases Tune Pancreatic Cancer Plasticity
    Article Snippet: Nuclei were isolated using lysis buffer [167.5 mM NaCl, 5 mM EDTA 264 pH 7.5, 50 mM Tris pH 7.5, 0.5% NP40, 1% Triton X-100, and protease inhibitors] and chromatin 265 was sonicated in 0.1% SDS sonication buffer using a Bioruptor Pico sonicator (Diagenode, 266 RRID: SCR_028774). .. Sheared chromatin was then immunoprecipitated with antibodies against 267 IgG (Cell Signalling #2729, RRID: AB_1031062), H3K4me3 (Millipore #05-745R, RRID: 268 AB_1587134), or H3K27me3 (Abcam #ab6002, RRID: AB_305237). .. DNA was purified using 269 MinElute PCR Purification Kits (QIAGEN) and quantitative PCR (qPCR) was performed to 270 measure DNA enrichment, using Power SYBR Green Master Mixes on a QuantStudio 7 Flex 271 Real-Time PCR system (Applied Biosystems).

    Article Title: YY1-NSUN7-m 5 C-HSPA1B axis drives polycystic ovary syndrome pathogenesis via epigenetic stabilization of HSPA1B mRNA.
    Article Snippet: Polycystic Ovary Syndrome (PCOS) is the most common endocrine disorder in reproductive-age women.. Heat shock 70 kDa protein 1B (HSPA1B) is critical for stress response and inflammation, but its role in PCOS remains unknown.. To validate HSPA1B as a key pathogenic gene in PCOS and investigate its regulatory mechanisms.

    Article Title: Brown Adipocyte BMAL1 Deficiency Is Associated with PVAT Remodeling and Increased Atherosclerosis.
    Article Snippet: .. Purified chromatin was immunoprecipitated using anti-Flag monoclonal antibody (CST, 14793S) and Normal Rabbit IgG (2729S, Cell Signalling Technology, Danvers, MA, USA). ..

    Lysis:

    Article Title: Circ_0000128 increases the progression of NSCLC through the miR-654-5p/OTUB1/Wnt pathway axis.
    Article Snippet: .. The lysis buffer was incubated with anti-AGO2 antibody (Cell Signalling Technology 2897) or isogenic IgG (Cell Signalling Technology 2729), after which protein A/G magnetic beads were added. ..

    Magnetic Beads:

    Article Title: Circ_0000128 increases the progression of NSCLC through the miR-654-5p/OTUB1/Wnt pathway axis.
    Article Snippet: .. The lysis buffer was incubated with anti-AGO2 antibody (Cell Signalling Technology 2897) or isogenic IgG (Cell Signalling Technology 2729), after which protein A/G magnetic beads were added. ..

    Purification:

    Article Title: Brown Adipocyte BMAL1 Deficiency Is Associated with PVAT Remodeling and Increased Atherosclerosis.
    Article Snippet: .. Purified chromatin was immunoprecipitated using anti-Flag monoclonal antibody (CST, 14793S) and Normal Rabbit IgG (2729S, Cell Signalling Technology, Danvers, MA, USA). ..

    Negative Control:

    Article Title: Apoptotic cell overload drives multinucleated giant cell formation after neoadjuvant chemotherapy in colorectal cancer: molecular mechanisms and tumor-suppressive effects
    Article Snippet: .. Antic-FOS antibody (#2250, CST, MA, USA) was used for immunoprecipitation, and normal rabbit IgG (#2729, CST, MA, USA) was used as the negative control. ..



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    ERMC proteins negatively regulate mitochondrial respiration and viral replication (A–C) Huh7.5 cells were transduced with lentiviruses expressing shRNAs which target the indicated proteins (MOI = 4). Four days later, cells were trypsinized, counted, and processed for measurements of various parameters of the oxygen consumption rate using the Seahorse technology as described in the section. Data were normalized to the mean basal OCR of the non-target shRNA (shNT) control conditions as in B–4E. ∗: p ≤ 0.05; ∗∗: p ≤ 0.01; Kruskal-Wallis test; N = 4–5. (D) Cells were transduced as in (a-c). Two days post-transduction, cells were infected with DENV-R2A reporter viruses which express Renilla reniformis luciferase (Rluc) at an MOI of 0.01. Two days post-infection, the luciferase activity was measured as a readout of viral replication and normalized to the shNT control condition. ∗∗∗: p ≤ 0.001; ∗∗: p ≤ 0.01; Kruskal-Wallis test; N = 6–10. (E–F) Two days post-transduction, cells were infected with DENV 16681s (E) or ZIKV H/PF/2013 (F) at a MOI of 0.1). 48 h later, the infectious titers of extracellular viral particles were determined by plaque assays. All values were normalized to the shNT condition. ∗∗: p ≤ 0.01; ∗: p ≤ 0.05; Kruskal-Wallis test; N = 3–6. (G and H) Primary human monocytes were transduced with lentiviruses encoding shNT, shSYNJ2BP or shRRBP1. 2 days post-transduction cells were infected with either DENV 16681s or ZIKV H/PF/2013 at an MOI of 1 in the presence of the panflaviviral anti-E antibody to enhance the infection. One day post-infection cells were collected and analyzed for their content in (G) RRBP1 and SYNJ2BP mRNAs and (H) viral RNAs using RT-qPCR. As control conditions, monocytes were treated with 10 μM of the NS5 RNA polymerase inhibitor NITD008 to demonstrate a productive replication in these cells. Mean values with standard deviations are shown.

    Journal: iScience

    Article Title: Dengue virus and Zika virus alter endoplasmic reticulum-mitochondria contact sites to regulate respiration and apoptosis

    doi: 10.1016/j.isci.2024.111599

    Figure Lengend Snippet: ERMC proteins negatively regulate mitochondrial respiration and viral replication (A–C) Huh7.5 cells were transduced with lentiviruses expressing shRNAs which target the indicated proteins (MOI = 4). Four days later, cells were trypsinized, counted, and processed for measurements of various parameters of the oxygen consumption rate using the Seahorse technology as described in the section. Data were normalized to the mean basal OCR of the non-target shRNA (shNT) control conditions as in B–4E. ∗: p ≤ 0.05; ∗∗: p ≤ 0.01; Kruskal-Wallis test; N = 4–5. (D) Cells were transduced as in (a-c). Two days post-transduction, cells were infected with DENV-R2A reporter viruses which express Renilla reniformis luciferase (Rluc) at an MOI of 0.01. Two days post-infection, the luciferase activity was measured as a readout of viral replication and normalized to the shNT control condition. ∗∗∗: p ≤ 0.001; ∗∗: p ≤ 0.01; Kruskal-Wallis test; N = 6–10. (E–F) Two days post-transduction, cells were infected with DENV 16681s (E) or ZIKV H/PF/2013 (F) at a MOI of 0.1). 48 h later, the infectious titers of extracellular viral particles were determined by plaque assays. All values were normalized to the shNT condition. ∗∗: p ≤ 0.01; ∗: p ≤ 0.05; Kruskal-Wallis test; N = 3–6. (G and H) Primary human monocytes were transduced with lentiviruses encoding shNT, shSYNJ2BP or shRRBP1. 2 days post-transduction cells were infected with either DENV 16681s or ZIKV H/PF/2013 at an MOI of 1 in the presence of the panflaviviral anti-E antibody to enhance the infection. One day post-infection cells were collected and analyzed for their content in (G) RRBP1 and SYNJ2BP mRNAs and (H) viral RNAs using RT-qPCR. As control conditions, monocytes were treated with 10 μM of the NS5 RNA polymerase inhibitor NITD008 to demonstrate a productive replication in these cells. Mean values with standard deviations are shown.

    Article Snippet: As replication control, NS5 inhibitor NITD008 (Tocris Small Molecules) was added at a final concentration of 10μM.

    Techniques: Transduction, Expressing, shRNA, Control, Infection, Luciferase, Activity Assay, Quantitative RT-PCR