Review



vector control  (Addgene inc)


Bioz Verified Symbol Addgene inc is a verified supplier  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
    • 95
      Buy from Supplier

    Structured Review

    Addgene inc vector control
    Vector Control, supplied by Addgene inc, used in various techniques. Bioz Stars score: 95/100, based on 185 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/control+empty+vector/pcDNA3%2E1-(empty)-TAG+(Plasmid+%23138209)/pm41830491-147-14-18
    Average 95 stars, based on 185 article reviews
    vector control - by Bioz Stars, 2026-11
    95/100 stars

    Images

    Related Articles

    Plasmid Preparation:

    Article Title: Fluorescence Lifetime-Based Line-Scanning FRET-FCCS for Simultaneous Analysis of Protein Binding and Diffusion on Membranes
    Article Snippet: Probing protein dynamics and interactions in cellular or subcellular membranes is vital to understanding life processes and regulatory mechanisms.. However, this remains challenging due to the temporal drift of the dynamic membrane and the inability to measure both accurate protein dynamics and protein−protein interactions.. To address this challenge, we developed line-scanning fluorescence resonance energy transfer and fluorescence cross-correlation spectroscopy (termed ls-FRET-FCCS), enabling the study of biomolecular dynamics and interactions on mitochondrial membranes simultaneously.

    Article Title: Decreased expression of circ-CCDC134 mediated by TNF-α in patients with rheumatoid arthritis affects T cell function via targeting protein phosphatase 2A.
    Article Snippet: .. Transfection study In alignment with our previous study, albeit with certain modifications [14], the plasmid pcDNA3.1(+) CircRNA Mini Vector, which encodes circ-CCDC134, as well as the plasmid pcDNA3.1 that encodes the linear form of CCDC134 (referred to as linear CCDC134) (both procured from the Addgene plasmid repository located in Watertown, Massachusetts, USA), or siRNA targeting junctional sequences of circ-CCDC134 (5 ́-AAACCTCTTGAGCATCCTTCAGCT-3 ́, Integrated DNA Technologies, Coralville, IA, USA) were employed to transfect Jurkat cells via electroporation. .. The control groups encompassed those cells that were ACCEPTED MANUSCRIPT AR TIC LE IN PR ES S ARTICLE IN PRESS transfected with the corresponding empty plasmid or siRNA control (NovoPro Bioscience, Shanghai, China).

    Article Title: Decreased expression of circ-CCDC134 mediated by TNF-α in patients with rheumatoid arthritis affects T cell function via targeting protein phosphatase 2A
    Article Snippet: .. In alignment with our previous study, albeit with certain modifications [ ], the plasmid pcDNA3.1(+) CircRNA Mini Vector, which encodes circ-CCDC134, as well as the plasmid pcDNA3.1 that encodes the linear form of CCDC134 (referred to as linear CCDC134) (both procured from the Addgene plasmid repository located in Watertown, Massachusetts, USA), or siRNA targeting junctional sequences of circ-CCDC134 (5 ́-AAACCTCTTGAGCATCCTTCAGCT-3 ́, Integrated DNA Technologies, Coralville, IA, USA) were employed to transfect Jurkat cells via electroporation. .. The control groups encompassed those cells that were transfected with the corresponding empty plasmid or siRNA control (NovoPro Bioscience, Shanghai, China).

    Article Title: Astrocytic PIEZO activation by heartbeat sound promotes extracellular matrix and maturation in human brain organoids
    Article Snippet: .. For calcium imaging in HEK293-P1KO cell line, cells were plated on imaging dishes (#81156, Ibidi) and co-transfected with 500 ng of pGP-CMV-GCaMP6f (#40755, Addgene, Watertown, USA) and CMV>mPiezo1:IRES:mScarlet3 (#VB241021-1228apt, Vector Builder Inc, Chicago, USA) or empty plasmid pcDNA3.1 (#138209, Addgene) as control. .. Transient transfections were performed with lipofectamine 3000 transfection reagent (#L3000015, Thermo Fisher Scientific, Oberhausen, Germany) following the manufacturer’s instructions.

    Article Title: Mirror-enhanced 4Pi-SMLM with one objective enables isotropic nanoscale imaging.
    Article Snippet: .. For microtubule labelling, oxStayGold-ALFA-Ensconsin was constructed by replacing the mEmerald sequence in mEmerald-Ensconsin with oxStayGold from pcDNA3/er-(n2)oxStayGold(c4) (Addgene, plasmid no. 185822) and inserting an ALFA tag at the N terminus of Ensconsin. ..

    Article Title: Small molecule JRMS modulating importin-β1 chaperone activity as a therapeutic strategy reducing TDP-43 pathology
    Article Snippet: At 70–80 % confluency, the culture medium was replaced with Opti-MEM (Gibco) and cells were transfected using a 1:2 ratio of plasmid DNA to EndoFectin Max transfection reagent (Genecopoeia), according to the manufacturer's instructions. .. The pcDNA3.1 constructs for transfection have been described previously, and include: EGFP, EGFP-TDP-25, EGFP-TDP-35, EGFP-TDP-43ΔNLS (K82A, R83A and K84A), EGFP-TDP-C-spl-272, RFP-TDP-25, 3 × FLAG-mTB-TDP-25 [ , ] and KPNB1-EGFP (Addgene plasmid # 106941). ..

    Article Title: Multi-Epitope DNA-Based Feline Immunodeficiency Virus Vaccine Construct Designed by Immunoinformatic and Machine Learning Tools as a Surrogate Model for HIV Vaccine Development
    Article Snippet: .. The plasmid pcDNA3.1+ was obtained from Addgene, and restriction enzymes EcoRI and XhoI were used to insert the peptide using Snapgene. ..

    Transfection:

    Article Title: Decreased expression of circ-CCDC134 mediated by TNF-α in patients with rheumatoid arthritis affects T cell function via targeting protein phosphatase 2A.
    Article Snippet: .. Transfection study In alignment with our previous study, albeit with certain modifications [14], the plasmid pcDNA3.1(+) CircRNA Mini Vector, which encodes circ-CCDC134, as well as the plasmid pcDNA3.1 that encodes the linear form of CCDC134 (referred to as linear CCDC134) (both procured from the Addgene plasmid repository located in Watertown, Massachusetts, USA), or siRNA targeting junctional sequences of circ-CCDC134 (5 ́-AAACCTCTTGAGCATCCTTCAGCT-3 ́, Integrated DNA Technologies, Coralville, IA, USA) were employed to transfect Jurkat cells via electroporation. .. The control groups encompassed those cells that were ACCEPTED MANUSCRIPT AR TIC LE IN PR ES S ARTICLE IN PRESS transfected with the corresponding empty plasmid or siRNA control (NovoPro Bioscience, Shanghai, China).

    Article Title: Small molecule JRMS modulating importin-β1 chaperone activity as a therapeutic strategy reducing TDP-43 pathology
    Article Snippet: At 70–80 % confluency, the culture medium was replaced with Opti-MEM (Gibco) and cells were transfected using a 1:2 ratio of plasmid DNA to EndoFectin Max transfection reagent (Genecopoeia), according to the manufacturer's instructions. .. The pcDNA3.1 constructs for transfection have been described previously, and include: EGFP, EGFP-TDP-25, EGFP-TDP-35, EGFP-TDP-43ΔNLS (K82A, R83A and K84A), EGFP-TDP-C-spl-272, RFP-TDP-25, 3 × FLAG-mTB-TDP-25 [ , ] and KPNB1-EGFP (Addgene plasmid # 106941). ..

    Electroporation:

    Article Title: Decreased expression of circ-CCDC134 mediated by TNF-α in patients with rheumatoid arthritis affects T cell function via targeting protein phosphatase 2A.
    Article Snippet: .. Transfection study In alignment with our previous study, albeit with certain modifications [14], the plasmid pcDNA3.1(+) CircRNA Mini Vector, which encodes circ-CCDC134, as well as the plasmid pcDNA3.1 that encodes the linear form of CCDC134 (referred to as linear CCDC134) (both procured from the Addgene plasmid repository located in Watertown, Massachusetts, USA), or siRNA targeting junctional sequences of circ-CCDC134 (5 ́-AAACCTCTTGAGCATCCTTCAGCT-3 ́, Integrated DNA Technologies, Coralville, IA, USA) were employed to transfect Jurkat cells via electroporation. .. The control groups encompassed those cells that were ACCEPTED MANUSCRIPT AR TIC LE IN PR ES S ARTICLE IN PRESS transfected with the corresponding empty plasmid or siRNA control (NovoPro Bioscience, Shanghai, China).

    Article Title: Decreased expression of circ-CCDC134 mediated by TNF-α in patients with rheumatoid arthritis affects T cell function via targeting protein phosphatase 2A
    Article Snippet: .. In alignment with our previous study, albeit with certain modifications [ ], the plasmid pcDNA3.1(+) CircRNA Mini Vector, which encodes circ-CCDC134, as well as the plasmid pcDNA3.1 that encodes the linear form of CCDC134 (referred to as linear CCDC134) (both procured from the Addgene plasmid repository located in Watertown, Massachusetts, USA), or siRNA targeting junctional sequences of circ-CCDC134 (5 ́-AAACCTCTTGAGCATCCTTCAGCT-3 ́, Integrated DNA Technologies, Coralville, IA, USA) were employed to transfect Jurkat cells via electroporation. .. The control groups encompassed those cells that were transfected with the corresponding empty plasmid or siRNA control (NovoPro Bioscience, Shanghai, China).

    Imaging:

    Article Title: Astrocytic PIEZO activation by heartbeat sound promotes extracellular matrix and maturation in human brain organoids
    Article Snippet: .. For calcium imaging in HEK293-P1KO cell line, cells were plated on imaging dishes (#81156, Ibidi) and co-transfected with 500 ng of pGP-CMV-GCaMP6f (#40755, Addgene, Watertown, USA) and CMV>mPiezo1:IRES:mScarlet3 (#VB241021-1228apt, Vector Builder Inc, Chicago, USA) or empty plasmid pcDNA3.1 (#138209, Addgene) as control. .. Transient transfections were performed with lipofectamine 3000 transfection reagent (#L3000015, Thermo Fisher Scientific, Oberhausen, Germany) following the manufacturer’s instructions.

    Control:

    Article Title: Astrocytic PIEZO activation by heartbeat sound promotes extracellular matrix and maturation in human brain organoids
    Article Snippet: .. For calcium imaging in HEK293-P1KO cell line, cells were plated on imaging dishes (#81156, Ibidi) and co-transfected with 500 ng of pGP-CMV-GCaMP6f (#40755, Addgene, Watertown, USA) and CMV>mPiezo1:IRES:mScarlet3 (#VB241021-1228apt, Vector Builder Inc, Chicago, USA) or empty plasmid pcDNA3.1 (#138209, Addgene) as control. .. Transient transfections were performed with lipofectamine 3000 transfection reagent (#L3000015, Thermo Fisher Scientific, Oberhausen, Germany) following the manufacturer’s instructions.

    Article Title: Fibroblast histone deacetylase-1 promotes kidney interstitial fibrosis following ischemia-reperfusion injury.
    Article Snippet: .. For overexpression of HDAC1, pcDNA3.1 plasmids 280 containing HDAC1-Flag (Addgene, 13820) or the empty vector control (pcDNA3.1 281 (Addgene, 138209) were used with the TransIT-X2 Dynamic Delivery System (Mirus 282 Bio, MIR 6005). .. Plasmid DNA was diluted in Opti-MEM I Reduced-Serum Medium 283 (Gibco, 31985062) to 1 μg/100 μl and combined with 2 μl of TransIT-X2 in an additional 284 100 μl Opti-MEM, followed by a 15-minute incubation at room temperature.

    Construct:

    Article Title: Mirror-enhanced 4Pi-SMLM with one objective enables isotropic nanoscale imaging.
    Article Snippet: .. For microtubule labelling, oxStayGold-ALFA-Ensconsin was constructed by replacing the mEmerald sequence in mEmerald-Ensconsin with oxStayGold from pcDNA3/er-(n2)oxStayGold(c4) (Addgene, plasmid no. 185822) and inserting an ALFA tag at the N terminus of Ensconsin. ..

    Article Title: Small molecule JRMS modulating importin-β1 chaperone activity as a therapeutic strategy reducing TDP-43 pathology
    Article Snippet: At 70–80 % confluency, the culture medium was replaced with Opti-MEM (Gibco) and cells were transfected using a 1:2 ratio of plasmid DNA to EndoFectin Max transfection reagent (Genecopoeia), according to the manufacturer's instructions. .. The pcDNA3.1 constructs for transfection have been described previously, and include: EGFP, EGFP-TDP-25, EGFP-TDP-35, EGFP-TDP-43ΔNLS (K82A, R83A and K84A), EGFP-TDP-C-spl-272, RFP-TDP-25, 3 × FLAG-mTB-TDP-25 [ , ] and KPNB1-EGFP (Addgene plasmid # 106941). ..

    Sequencing:

    Article Title: Mirror-enhanced 4Pi-SMLM with one objective enables isotropic nanoscale imaging.
    Article Snippet: .. For microtubule labelling, oxStayGold-ALFA-Ensconsin was constructed by replacing the mEmerald sequence in mEmerald-Ensconsin with oxStayGold from pcDNA3/er-(n2)oxStayGold(c4) (Addgene, plasmid no. 185822) and inserting an ALFA tag at the N terminus of Ensconsin. ..

    Over Expression:

    Article Title: Fibroblast histone deacetylase-1 promotes kidney interstitial fibrosis following ischemia-reperfusion injury.
    Article Snippet: .. For overexpression of HDAC1, pcDNA3.1 plasmids 280 containing HDAC1-Flag (Addgene, 13820) or the empty vector control (pcDNA3.1 281 (Addgene, 138209) were used with the TransIT-X2 Dynamic Delivery System (Mirus 282 Bio, MIR 6005). .. Plasmid DNA was diluted in Opti-MEM I Reduced-Serum Medium 283 (Gibco, 31985062) to 1 μg/100 μl and combined with 2 μl of TransIT-X2 in an additional 284 100 μl Opti-MEM, followed by a 15-minute incubation at room temperature.



    Similar Products

    empty vector control  (OriGene)
    94
    OriGene empty vector control
    Empty Vector Control, supplied by OriGene, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/control+empty+vector/Parp11+(NM_181402)+Mouse+Tagged+ORF+Clone/bio_rxiv__64898__2026__06__01__729331-208-6-12
    Average 94 stars, based on 1 article reviews
    empty vector control - by Bioz Stars, 2026-11
    94/100 stars
    		  Buy from Supplier
    egfp empty vector control  (TaKaRa)
    95
    TaKaRa egfp empty vector control
    a, HCT116 cells stably expressing BRIP1 R162Q were <t>transiently</t> <t>transfected</t> using PEI with empty vector <t>(EV-EGFP)</t> or RNaseH1-WT-eGFP. Transfection efficiency was assessed via EGFP fluorescence. b, immunoblot analysis confirming expression of GFP-tagged constructs in transfected cells. c, Immunofluorescence detection of R-loops using the S9.6 antibody in cells transfected with EGFP or EGFP-RNaseH1. d, Quantification of S9.6 mean fluorescence intensity (MFI) per nucleus. Data represent mean ± SEM of three independent experiments. e, Immunofluorescence detection of G-quadruplex (G4) structures using the BG4 antibody in cells transfected with EGFP or EGFP-RNaseH1. f, Quantification of BG4 fluorescence intensity per nucleus. Data represent mean ± SEM of three independent experiments. g, Representative DNA fiber assay images from HCT116 cells harboring BRIP1 R162Q transfected with empty vector (EV-eGFP) or RNaseH1-WT. DNA replication tracts were sequentially labeled with CldU (red) and IdU (green). h, Quantification of DNA fiber types. RNaseH1-WT expression reduced the proportion of stalled replication forks in cells harboring BRIP1 R162Q. i, While the overall fork speed was not significantly increased upon RNaseH1-WT expression, tracts displayed a more balanced distribution of CldU and IdU incorporation. j, Fork asymmetry analysis. RNaseH1-WT expression reduced fork asymmetry, indicating that left- and right-moving sister forks progressed at more similar speeds. Labeled tracks were visualized by confocal microscopy, and tract lengths were measured on raw LSM images. Lengths (µm) were converted to replication speed (kb/min) using a factor of 2.59 kb/µm. All data represent mean ± SEM from three independent experiments. At least 150 fibers per condition were analyzed in each replicate. Statistical significance was determined using paired two-tailed Student’s t-test (*p < 0.05, **p < 0.01, ***p < 0.001, ns = not significant). c, e, Representative confocal images are shown.
    Egfp Empty Vector Control, supplied by TaKaRa, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/control+empty+vector/pAcGFP1-C1+Vector/bio_rxiv__64898__2026__03__24__714005-201-9-14
    Average 95 stars, based on 1 article reviews
    egfp empty vector control - by Bioz Stars, 2026-11
    95/100 stars
    		  Buy from Supplier
    vector control  (Addgene inc)
    95
    Addgene inc vector control
    a, HCT116 cells stably expressing BRIP1 R162Q were <t>transiently</t> <t>transfected</t> using PEI with empty vector <t>(EV-EGFP)</t> or RNaseH1-WT-eGFP. Transfection efficiency was assessed via EGFP fluorescence. b, immunoblot analysis confirming expression of GFP-tagged constructs in transfected cells. c, Immunofluorescence detection of R-loops using the S9.6 antibody in cells transfected with EGFP or EGFP-RNaseH1. d, Quantification of S9.6 mean fluorescence intensity (MFI) per nucleus. Data represent mean ± SEM of three independent experiments. e, Immunofluorescence detection of G-quadruplex (G4) structures using the BG4 antibody in cells transfected with EGFP or EGFP-RNaseH1. f, Quantification of BG4 fluorescence intensity per nucleus. Data represent mean ± SEM of three independent experiments. g, Representative DNA fiber assay images from HCT116 cells harboring BRIP1 R162Q transfected with empty vector (EV-eGFP) or RNaseH1-WT. DNA replication tracts were sequentially labeled with CldU (red) and IdU (green). h, Quantification of DNA fiber types. RNaseH1-WT expression reduced the proportion of stalled replication forks in cells harboring BRIP1 R162Q. i, While the overall fork speed was not significantly increased upon RNaseH1-WT expression, tracts displayed a more balanced distribution of CldU and IdU incorporation. j, Fork asymmetry analysis. RNaseH1-WT expression reduced fork asymmetry, indicating that left- and right-moving sister forks progressed at more similar speeds. Labeled tracks were visualized by confocal microscopy, and tract lengths were measured on raw LSM images. Lengths (µm) were converted to replication speed (kb/min) using a factor of 2.59 kb/µm. All data represent mean ± SEM from three independent experiments. At least 150 fibers per condition were analyzed in each replicate. Statistical significance was determined using paired two-tailed Student’s t-test (*p < 0.05, **p < 0.01, ***p < 0.001, ns = not significant). c, e, Representative confocal images are shown.
    Vector Control, supplied by Addgene inc, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/control+empty+vector/pcDNA3%2E1-(empty)-TAG+(Plasmid+%23138209)/pm41830491-147-14-18
    Average 95 stars, based on 1 article reviews
    vector control - by Bioz Stars, 2026-11
    95/100 stars
    		  Buy from Supplier
    control empty vectors nc  (Genechem)
    86
    Genechem control empty vectors nc
    Overexpression of SNHG1 or HDAC6 partially reverses the pro-angiogenic effect of EPC-EVs on MAECs. MAECs were transfected with SNHG1 overexpression <t>vectors</t> (oe-SNHG1), HDAC6 overexpression vectors (oe-HDAC6), or <t>empty</t> vectors (oe-NC, <t>control)</t> in HG (30 mM) medium. A : Transfection efficiency of oe-SNHG1 and oe-HDAC6 was verified by qRT-PCR (n = 3). Cells were then co-treated with EPC-EVs-miR in HG medium. B : SNHG1 and HDAC6 expression was detected by qRT-PCR (n = 3). C : Expression of HDAC6, CD31, and VEGFA was measured by Western blot (n = 3). D : Cell viability was assessed by CCK-8 assay (n = 3). E : Angiogenic capacity was evaluated by tube formation assay (n = 3). F : Cell migration was measured by scratch wound healing assay (n = 3). Data are presented as mean ± standard deviation. Data in panel A were analyze using student’s t-test. Data in panels B , D , E , and F were analyzed using one-way ANOVA. Data in panel C were analyzed using two-way ANOVA, followed by Tukey’s multiple comparisons test. * p < 0.05 and ** p < 0.01
    Control Empty Vectors Nc, supplied by Genechem, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/control+empty+vector/control+empty+nc+vectors/pmc12827335-61-13-17
    Average 86 stars, based on 1 article reviews
    control empty vectors nc - by Bioz Stars, 2026-11
    86/100 stars
    		  Buy from Supplier
    pcdh ef1 fhc empty vector control  (Addgene inc)
    94
    Addgene inc pcdh ef1 fhc empty vector control
    Overexpression of SNHG1 or HDAC6 partially reverses the pro-angiogenic effect of EPC-EVs on MAECs. MAECs were transfected with SNHG1 overexpression <t>vectors</t> (oe-SNHG1), HDAC6 overexpression vectors (oe-HDAC6), or <t>empty</t> vectors (oe-NC, <t>control)</t> in HG (30 mM) medium. A : Transfection efficiency of oe-SNHG1 and oe-HDAC6 was verified by qRT-PCR (n = 3). Cells were then co-treated with EPC-EVs-miR in HG medium. B : SNHG1 and HDAC6 expression was detected by qRT-PCR (n = 3). C : Expression of HDAC6, CD31, and VEGFA was measured by Western blot (n = 3). D : Cell viability was assessed by CCK-8 assay (n = 3). E : Angiogenic capacity was evaluated by tube formation assay (n = 3). F : Cell migration was measured by scratch wound healing assay (n = 3). Data are presented as mean ± standard deviation. Data in panel A were analyze using student’s t-test. Data in panels B , D , E , and F were analyzed using one-way ANOVA. Data in panel C were analyzed using two-way ANOVA, followed by Tukey’s multiple comparisons test. * p < 0.05 and ** p < 0.01
    Pcdh Ef1 Fhc Empty Vector Control, supplied by Addgene inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/control+empty+vector/pCDH-EF1-FHC+(Plasmid+%2364874)/pmc12758111-80-8-12
    Average 94 stars, based on 1 article reviews
    pcdh ef1 fhc empty vector control - by Bioz Stars, 2026-11
    94/100 stars
    		  Buy from Supplier
    empty vector control  (New England Biolabs)
    99
    New England Biolabs empty vector control
    Overexpression of SNHG1 or HDAC6 partially reverses the pro-angiogenic effect of EPC-EVs on MAECs. MAECs were transfected with SNHG1 overexpression <t>vectors</t> (oe-SNHG1), HDAC6 overexpression vectors (oe-HDAC6), or <t>empty</t> vectors (oe-NC, <t>control)</t> in HG (30 mM) medium. A : Transfection efficiency of oe-SNHG1 and oe-HDAC6 was verified by qRT-PCR (n = 3). Cells were then co-treated with EPC-EVs-miR in HG medium. B : SNHG1 and HDAC6 expression was detected by qRT-PCR (n = 3). C : Expression of HDAC6, CD31, and VEGFA was measured by Western blot (n = 3). D : Cell viability was assessed by CCK-8 assay (n = 3). E : Angiogenic capacity was evaluated by tube formation assay (n = 3). F : Cell migration was measured by scratch wound healing assay (n = 3). Data are presented as mean ± standard deviation. Data in panel A were analyze using student’s t-test. Data in panels B , D , E , and F were analyzed using one-way ANOVA. Data in panel C were analyzed using two-way ANOVA, followed by Tukey’s multiple comparisons test. * p < 0.05 and ** p < 0.01
    Empty Vector Control, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/control+empty+vector/Q5+Site-Directed+Mutagenesis+Kit/bio_rxiv__2025__11__13__688230-150-8-19
    Average 99 stars, based on 1 article reviews
    empty vector control - by Bioz Stars, 2026-11
    99/100 stars
    		  Buy from Supplier
    empty vector control  (Addgene inc)
    93
    Addgene inc empty vector control
    Overexpression of SNHG1 or HDAC6 partially reverses the pro-angiogenic effect of EPC-EVs on MAECs. MAECs were transfected with SNHG1 overexpression <t>vectors</t> (oe-SNHG1), HDAC6 overexpression vectors (oe-HDAC6), or <t>empty</t> vectors (oe-NC, <t>control)</t> in HG (30 mM) medium. A : Transfection efficiency of oe-SNHG1 and oe-HDAC6 was verified by qRT-PCR (n = 3). Cells were then co-treated with EPC-EVs-miR in HG medium. B : SNHG1 and HDAC6 expression was detected by qRT-PCR (n = 3). C : Expression of HDAC6, CD31, and VEGFA was measured by Western blot (n = 3). D : Cell viability was assessed by CCK-8 assay (n = 3). E : Angiogenic capacity was evaluated by tube formation assay (n = 3). F : Cell migration was measured by scratch wound healing assay (n = 3). Data are presented as mean ± standard deviation. Data in panel A were analyze using student’s t-test. Data in panels B , D , E , and F were analyzed using one-way ANOVA. Data in panel C were analyzed using two-way ANOVA, followed by Tukey’s multiple comparisons test. * p < 0.05 and ** p < 0.01
    Empty Vector Control, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/control+empty+vector/pcDNA3%2E2+GW+delCMV+(Plasmid+%2329496)/bio_rxiv__2025__10__29__685456-55-18-21
    Average 93 stars, based on 1 article reviews
    empty vector control - by Bioz Stars, 2026-11
    93/100 stars
    		  Buy from Supplier
    control empty vector plvx ires zsgreen1  (TaKaRa)
    96
    TaKaRa control empty vector plvx ires zsgreen1
    Overexpression of SNHG1 or HDAC6 partially reverses the pro-angiogenic effect of EPC-EVs on MAECs. MAECs were transfected with SNHG1 overexpression <t>vectors</t> (oe-SNHG1), HDAC6 overexpression vectors (oe-HDAC6), or <t>empty</t> vectors (oe-NC, <t>control)</t> in HG (30 mM) medium. A : Transfection efficiency of oe-SNHG1 and oe-HDAC6 was verified by qRT-PCR (n = 3). Cells were then co-treated with EPC-EVs-miR in HG medium. B : SNHG1 and HDAC6 expression was detected by qRT-PCR (n = 3). C : Expression of HDAC6, CD31, and VEGFA was measured by Western blot (n = 3). D : Cell viability was assessed by CCK-8 assay (n = 3). E : Angiogenic capacity was evaluated by tube formation assay (n = 3). F : Cell migration was measured by scratch wound healing assay (n = 3). Data are presented as mean ± standard deviation. Data in panel A were analyze using student’s t-test. Data in panels B , D , E , and F were analyzed using one-way ANOVA. Data in panel C were analyzed using two-way ANOVA, followed by Tukey’s multiple comparisons test. * p < 0.05 and ** p < 0.01
    Control Empty Vector Plvx Ires Zsgreen1, supplied by TaKaRa, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/control+empty+vector/pLVX-IRES-ZsGreen1+Vector/pmc12403285__40364_2025_815_MOESM1_ESM-80-26-30
    Average 96 stars, based on 1 article reviews
    control empty vector plvx ires zsgreen1 - by Bioz Stars, 2026-11
    96/100 stars
    		  Buy from Supplier
    control empty vector  (Addgene inc)
    93
    Addgene inc control empty vector
    Overexpression of SNHG1 or HDAC6 partially reverses the pro-angiogenic effect of EPC-EVs on MAECs. MAECs were transfected with SNHG1 overexpression <t>vectors</t> (oe-SNHG1), HDAC6 overexpression vectors (oe-HDAC6), or <t>empty</t> vectors (oe-NC, <t>control)</t> in HG (30 mM) medium. A : Transfection efficiency of oe-SNHG1 and oe-HDAC6 was verified by qRT-PCR (n = 3). Cells were then co-treated with EPC-EVs-miR in HG medium. B : SNHG1 and HDAC6 expression was detected by qRT-PCR (n = 3). C : Expression of HDAC6, CD31, and VEGFA was measured by Western blot (n = 3). D : Cell viability was assessed by CCK-8 assay (n = 3). E : Angiogenic capacity was evaluated by tube formation assay (n = 3). F : Cell migration was measured by scratch wound healing assay (n = 3). Data are presented as mean ± standard deviation. Data in panel A were analyze using student’s t-test. Data in panels B , D , E , and F were analyzed using one-way ANOVA. Data in panel C were analyzed using two-way ANOVA, followed by Tukey’s multiple comparisons test. * p < 0.05 and ** p < 0.01
    Control Empty Vector, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/control+empty+vector/plenti-ATF4+(Plasmid+%23125238)/pmc12281011-54-1-4
    Average 93 stars, based on 1 article reviews
    control empty vector - by Bioz Stars, 2026-11
    93/100 stars
    		  Buy from Supplier

    Image Search Results


    a, HCT116 cells stably expressing BRIP1 R162Q were transiently transfected using PEI with empty vector (EV-EGFP) or RNaseH1-WT-eGFP. Transfection efficiency was assessed via EGFP fluorescence. b, immunoblot analysis confirming expression of GFP-tagged constructs in transfected cells. c, Immunofluorescence detection of R-loops using the S9.6 antibody in cells transfected with EGFP or EGFP-RNaseH1. d, Quantification of S9.6 mean fluorescence intensity (MFI) per nucleus. Data represent mean ± SEM of three independent experiments. e, Immunofluorescence detection of G-quadruplex (G4) structures using the BG4 antibody in cells transfected with EGFP or EGFP-RNaseH1. f, Quantification of BG4 fluorescence intensity per nucleus. Data represent mean ± SEM of three independent experiments. g, Representative DNA fiber assay images from HCT116 cells harboring BRIP1 R162Q transfected with empty vector (EV-eGFP) or RNaseH1-WT. DNA replication tracts were sequentially labeled with CldU (red) and IdU (green). h, Quantification of DNA fiber types. RNaseH1-WT expression reduced the proportion of stalled replication forks in cells harboring BRIP1 R162Q. i, While the overall fork speed was not significantly increased upon RNaseH1-WT expression, tracts displayed a more balanced distribution of CldU and IdU incorporation. j, Fork asymmetry analysis. RNaseH1-WT expression reduced fork asymmetry, indicating that left- and right-moving sister forks progressed at more similar speeds. Labeled tracks were visualized by confocal microscopy, and tract lengths were measured on raw LSM images. Lengths (µm) were converted to replication speed (kb/min) using a factor of 2.59 kb/µm. All data represent mean ± SEM from three independent experiments. At least 150 fibers per condition were analyzed in each replicate. Statistical significance was determined using paired two-tailed Student’s t-test (*p < 0.05, **p < 0.01, ***p < 0.001, ns = not significant). c, e, Representative confocal images are shown.

    Journal: bioRxiv

    Article Title: Role of a childhood cancer-linked BRIP1/FANCJ germline variant in genomic instability and cancer cell vulnerability

    doi: 10.64898/2026.03.24.714005

    Figure Lengend Snippet: a, HCT116 cells stably expressing BRIP1 R162Q were transiently transfected using PEI with empty vector (EV-EGFP) or RNaseH1-WT-eGFP. Transfection efficiency was assessed via EGFP fluorescence. b, immunoblot analysis confirming expression of GFP-tagged constructs in transfected cells. c, Immunofluorescence detection of R-loops using the S9.6 antibody in cells transfected with EGFP or EGFP-RNaseH1. d, Quantification of S9.6 mean fluorescence intensity (MFI) per nucleus. Data represent mean ± SEM of three independent experiments. e, Immunofluorescence detection of G-quadruplex (G4) structures using the BG4 antibody in cells transfected with EGFP or EGFP-RNaseH1. f, Quantification of BG4 fluorescence intensity per nucleus. Data represent mean ± SEM of three independent experiments. g, Representative DNA fiber assay images from HCT116 cells harboring BRIP1 R162Q transfected with empty vector (EV-eGFP) or RNaseH1-WT. DNA replication tracts were sequentially labeled with CldU (red) and IdU (green). h, Quantification of DNA fiber types. RNaseH1-WT expression reduced the proportion of stalled replication forks in cells harboring BRIP1 R162Q. i, While the overall fork speed was not significantly increased upon RNaseH1-WT expression, tracts displayed a more balanced distribution of CldU and IdU incorporation. j, Fork asymmetry analysis. RNaseH1-WT expression reduced fork asymmetry, indicating that left- and right-moving sister forks progressed at more similar speeds. Labeled tracks were visualized by confocal microscopy, and tract lengths were measured on raw LSM images. Lengths (µm) were converted to replication speed (kb/min) using a factor of 2.59 kb/µm. All data represent mean ± SEM from three independent experiments. At least 150 fibers per condition were analyzed in each replicate. Statistical significance was determined using paired two-tailed Student’s t-test (*p < 0.05, **p < 0.01, ***p < 0.001, ns = not significant). c, e, Representative confocal images are shown.

    Article Snippet: For R-loop removal, cells were transiently transfected with an EGFP empty vector control (pEGFP-C1, Takara Bio, #632470), EGFP-tagged RNaseH1 wild-type (WT), or the catalytically inactive RNaseH1 D145N mutant as indicated.

    Techniques: Stable Transfection, Expressing, Transfection, Plasmid Preparation, Fluorescence, Western Blot, Construct, Immunofluorescence, Labeling, Confocal Microscopy, Two Tailed Test

    Overexpression of SNHG1 or HDAC6 partially reverses the pro-angiogenic effect of EPC-EVs on MAECs. MAECs were transfected with SNHG1 overexpression vectors (oe-SNHG1), HDAC6 overexpression vectors (oe-HDAC6), or empty vectors (oe-NC, control) in HG (30 mM) medium. A : Transfection efficiency of oe-SNHG1 and oe-HDAC6 was verified by qRT-PCR (n = 3). Cells were then co-treated with EPC-EVs-miR in HG medium. B : SNHG1 and HDAC6 expression was detected by qRT-PCR (n = 3). C : Expression of HDAC6, CD31, and VEGFA was measured by Western blot (n = 3). D : Cell viability was assessed by CCK-8 assay (n = 3). E : Angiogenic capacity was evaluated by tube formation assay (n = 3). F : Cell migration was measured by scratch wound healing assay (n = 3). Data are presented as mean ± standard deviation. Data in panel A were analyze using student’s t-test. Data in panels B , D , E , and F were analyzed using one-way ANOVA. Data in panel C were analyzed using two-way ANOVA, followed by Tukey’s multiple comparisons test. * p < 0.05 and ** p < 0.01

    Journal: Cell Biology and Toxicology

    Article Title: Endothelial progenitor cell derived extracellular vesicles promotes wound healing in diabetic mice via activating mobilization and neovascularization

    doi: 10.1007/s10565-025-10134-3

    Figure Lengend Snippet: Overexpression of SNHG1 or HDAC6 partially reverses the pro-angiogenic effect of EPC-EVs on MAECs. MAECs were transfected with SNHG1 overexpression vectors (oe-SNHG1), HDAC6 overexpression vectors (oe-HDAC6), or empty vectors (oe-NC, control) in HG (30 mM) medium. A : Transfection efficiency of oe-SNHG1 and oe-HDAC6 was verified by qRT-PCR (n = 3). Cells were then co-treated with EPC-EVs-miR in HG medium. B : SNHG1 and HDAC6 expression was detected by qRT-PCR (n = 3). C : Expression of HDAC6, CD31, and VEGFA was measured by Western blot (n = 3). D : Cell viability was assessed by CCK-8 assay (n = 3). E : Angiogenic capacity was evaluated by tube formation assay (n = 3). F : Cell migration was measured by scratch wound healing assay (n = 3). Data are presented as mean ± standard deviation. Data in panel A were analyze using student’s t-test. Data in panels B , D , E , and F were analyzed using one-way ANOVA. Data in panel C were analyzed using two-way ANOVA, followed by Tukey’s multiple comparisons test. * p < 0.05 and ** p < 0.01

    Article Snippet: For lentiviral infection, GFP-labeled miR-204-5p lentiviral overexpression vectors, SNHG1 lentiviral overexpression vectors, or control empty vectors (NC) (Genechem, Shanghai, China) were applied to transfection with 293 T cells (CRL-1573; ATCC, Manassas, VA, USA).

    Techniques: Over Expression, Transfection, Control, Quantitative RT-PCR, Expressing, Western Blot, CCK-8 Assay, Tube Formation Assay, Migration, Wound Healing Assay, Standard Deviation