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cdna amplicon size (approximately 400 bp) for library construction  (10X Genomics)

 
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    10X Genomics cdna amplicon size (approximately 400 bp) for library construction
    Cdna Amplicon Size (Approximately 400 Bp) For Library Construction, supplied by 10X Genomics, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/constructive+approximation/cdna+amplicon+size/pmc11584403-358-22-24
    Average 90 stars, based on 1 article reviews
    cdna amplicon size (approximately 400 bp) for library construction - by Bioz Stars, 2026-11
    90/100 stars

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    Amplification:

    Article Title: Evolution and structure of clinically relevant gene fusions in multiple myeloma
    Article Snippet: .. Full length cDNA from poly-A mRNA transcripts was enzymatically fragmented and size selected to optimize the cDNA amplicon size (approximately 400 bp) for library construction (10x Genomics). .. The concentration of the 10x single cell library was accurately determined through qPCR (Kapa Biosystems) to produce cluster counts appropriate for the HiSeq 4000 or NovaSeq 6000 platform (Illumina).

    Article Title: Differential chromatin accessibility and transcriptional dynamics define breast cancer subtypes and their lineages
    Article Snippet: .. Full-length cDNA from poly-A mRNA transcripts was enzymatically fragmented and size selected to optimize the cDNA amplicon size (approximately 400 bp) for library construction (10x Genomics). .. The concentration of the 10x single-cell library was accurately determined through qPCR (Kapa Biosystems) to produce cluster counts appropriate for the HiSeq 4000 or NovaSeq-6000 platform (Illumina).



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    Distribution and sequence coverage of Tn5 mutants in the S. praecaptivus genome are depicted as a histogram (A, Upper). Note that mutations affecting Sant_4061 are the most abundant in the library, following growth in LB media. The zoomed in regions of the histogram (A, Lower) reveal the distribution and abundance of Tn5 mutants in the genomic regions encoding phoPQ and Sant_4061 (in red), with the mutants recovered in the screen for polymyxin B sensitivity highlighted in orange, with the number of recovered mutants shown above each column. Growth curves for WT S. praecaptivus and Sant_4061 <t>and</t> <t>phoP</t> mutants grown in either LB or LB + Polymyxin B (PB) for 10 hours (B). Standard errors are shown above and below the average of three replicates at each time point. ΔSant_4061, ΔphoQ, and ΔphoP are unable to grow on LB supplemented with polymyxin B (C).
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    Distribution and sequence coverage of Tn5 mutants in the S. praecaptivus genome are depicted as a histogram (A, Upper). Note that mutations affecting Sant_4061 are the most abundant in the library, following growth in LB media. The zoomed in regions of the histogram (A, Lower) reveal the distribution and abundance of Tn5 mutants in the genomic regions encoding phoPQ and Sant_4061 (in red), with the mutants recovered in the screen for polymyxin B sensitivity highlighted in orange, with the number of recovered mutants shown above each column. Growth curves for WT S. praecaptivus and Sant_4061 and phoP mutants grown in either LB or LB + Polymyxin B (PB) for 10 hours (B). Standard errors are shown above and below the average of three replicates at each time point. ΔSant_4061, ΔphoQ, and ΔphoP are unable to grow on LB supplemented with polymyxin B (C).

    Journal: Molecular microbiology

    Article Title: The Regulation of Antimicrobial Peptide Resistance in the Transition to Insect Symbiosis

    doi: 10.1111/mmi.13598

    Figure Lengend Snippet: Distribution and sequence coverage of Tn5 mutants in the S. praecaptivus genome are depicted as a histogram (A, Upper). Note that mutations affecting Sant_4061 are the most abundant in the library, following growth in LB media. The zoomed in regions of the histogram (A, Lower) reveal the distribution and abundance of Tn5 mutants in the genomic regions encoding phoPQ and Sant_4061 (in red), with the mutants recovered in the screen for polymyxin B sensitivity highlighted in orange, with the number of recovered mutants shown above each column. Growth curves for WT S. praecaptivus and Sant_4061 and phoP mutants grown in either LB or LB + Polymyxin B (PB) for 10 hours (B). Standard errors are shown above and below the average of three replicates at each time point. ΔSant_4061, ΔphoQ, and ΔphoP are unable to grow on LB supplemented with polymyxin B (C).

    Article Snippet: Construction of phoP and phoQ knockouts A plasmid construct consisting of approximately 500 bp of homologous sequence up and downstream of phoP with an internal kanamycin cassette was commercially produced (Genscript).

    Techniques: Sequencing

    Phylogeny of S. praecaptivus and related Sodalis-allied endosymbionts and free-living bacteria based on maximum likelihood analyses of the phoQ coding sequence (1.45 kbp) and 16S rRNA (1.46 kbp). Sequences below strain names on the phoQ phylogeny show the amino acids sequences of the Mg2+-binding site within PhoQ, with acidic residues highlighted in bold. The numbers adjacent to nodes indicate maximum likelihood bootstrap values shown for nodes with bootstrap support > 80%.

    Journal: Molecular microbiology

    Article Title: The Regulation of Antimicrobial Peptide Resistance in the Transition to Insect Symbiosis

    doi: 10.1111/mmi.13598

    Figure Lengend Snippet: Phylogeny of S. praecaptivus and related Sodalis-allied endosymbionts and free-living bacteria based on maximum likelihood analyses of the phoQ coding sequence (1.45 kbp) and 16S rRNA (1.46 kbp). Sequences below strain names on the phoQ phylogeny show the amino acids sequences of the Mg2+-binding site within PhoQ, with acidic residues highlighted in bold. The numbers adjacent to nodes indicate maximum likelihood bootstrap values shown for nodes with bootstrap support > 80%.

    Article Snippet: Construction of phoP and phoQ knockouts A plasmid construct consisting of approximately 500 bp of homologous sequence up and downstream of phoP with an internal kanamycin cassette was commercially produced (Genscript).

    Techniques: Bacteria, Sequencing, Binding Assay

    Bacterial strains used in this study

    Journal: Molecular microbiology

    Article Title: The Regulation of Antimicrobial Peptide Resistance in the Transition to Insect Symbiosis

    doi: 10.1111/mmi.13598

    Figure Lengend Snippet: Bacterial strains used in this study

    Article Snippet: Construction of phoP and phoQ knockouts A plasmid construct consisting of approximately 500 bp of homologous sequence up and downstream of phoP with an internal kanamycin cassette was commercially produced (Genscript).

    Techniques: