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computerized morphometry analysis  (Definiens Inc)

 
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    Definiens Inc computerized morphometry analysis
    Computerized Morphometry Analysis, supplied by Definiens Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/computerized+morphometry+analysis+system/computerized+morphometry+analysis/pmc04411181-100-12-17
    Average 90 stars, based on 1 article reviews
    computerized morphometry analysis - by Bioz Stars, 2026-10
    90/100 stars

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    Staining:

    Article Title: Remodeling of Stellate Ganglion Neurons following Spatially Targeted Myocardial Infarction: Neuropeptide and Morphologic Changes
    Article Snippet: Stellate ganglia were removed prior to sacrifice, rinsed, and immediately transferred to cold 10% phosphate-buffered formalin (Fisher Scientific, Pittsburgh, PA) for 5-7days, and then to cold 70% ethanol (Sigma-Aldrich, St Louis, MO) until embedding (< 1 week). .. Neuronal size was determined from Thionin staining (Fisher Scientific, Pittsburgh, PA) using computerized morphometry analysis (Tissue Studio, Definiens Inc., Parsippany, NJ) as previously published 9 . .. Adrenergic phenotype was quantified using anti-tyrosine hydroxylase (-TH) antibody (1:200 dilution, Abcam, Cat# ab112, Cambridge, MA, USA) and cholinergic phenotype by anti-choline acetyltransferease antibody (1:100 dilution, Abcam, Cat# ab18736, Cambridge, MA, USA), both detected by diaminobenzidine (DAB, Life Technologies, Green Island, NY) per manufacturer's recommended protocol.

    Article Title: Remodeling of Stellate Ganglion Neurons following Spatially Targeted Myocardial Infarction: Neuropeptide and Morphologic Changes
    Article Snippet: Tissue Handling Stellate ganglia were removed prior to sacrifice, rinsed, and immediately transferred to cold 10% phosphate-buffered formalin (Fisher Scientific, Pittsburgh, PA) for 5-7days, and then to cold 70% ethanol (Sigma-Aldrich, St Louis, MO) until embedding (< 1 week). .. Histologic Stains Neuronal size was determined from Thionin staining (Fisher Scientific, Pittsburgh, PA) using computerized morphometry analysis (Tissue Studio, Definiens Inc., Parsippany, NJ) as previously published 9 . .. Immunohistochemical Stains Adrenergic phenotype was quantified using anti-tyrosine hydroxylase (-TH) antibody (1:200 dilution, Abcam, Cat# ab112, Cambridge, MA, USA) and cholinergic phenotype by anti-choline acetyltransferease antibody (1:100 dilution, Abcam, Cat# ab18736, Cambridge, MA, USA), both detected by diaminobenzidine (DAB, Life Technologies, Green Island, NY) per manufacturer's recommended protocol.



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