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ERITHACUS SOFTWARE LIMITED computer program grafit
Computer Program Grafit, supplied by ERITHACUS SOFTWARE LIMITED, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/computer+software+program/computer+program+grafit/us07183093-829-1-7
Average 90 stars, based on 1 article reviews
computer program grafit - by Bioz Stars, 2026-09
90/100 stars

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Article Title: A novel glycoside hydrolase family 97 enzyme: Bifunctional β-l-arabinopyranosidase/α-galactosidase from Bacteroides thetaiotaomicron.
Article Snippet: The kinetic constants were calculated by fitting the 215 Michaelis-Menten equation by nonlinear regression using the computer program GraFit 7 216 (Erithacus Software Ltd., Surrey, UK).

Article Title: The Interaction of Schistosoma Japonicum Glutathione Transferase with Cibacron Blue 3GA and its Fragments.
Article Snippet: Laboratory of Enzyme Technology, Department of Biotechnology, School of Applied Biology and Biotechnology, Agricultural University of Athens, 75 Iera Odos Street, GR-11855-Athens, Greece; Laboratory of Genetics, Department of Biotechnology, School of Applied Biology and Biotechnology, Agricultural University of Athens, 75 Iera Odos Street, GR-11855-Athens, Greece; Department of Pharmacognosy, College of Pharmacy, Prince Sattam Bin Abdulaziz University, 11942 Al Kharj, Saudi Arabia; Department of Pharmaceutics, College of Pharmacy, Prince Sattam Bin Abdulaziz University, 11942, Alkharj, Saudi Arabia; Department of Pharmacognosy, College of Pharmacy, Prince Sattam Bin Abdulaziz University, 11942, Alkharj, Saudi Arabia; Turku Bioscience Centre, University of Turku and Åbo Akademi University, Turku 20521, Finland

Article Title: Directed evolution of glutathione transferases towards a selective glutathione-binding site and improved oxidative stability.
Article Snippet: Article history: Received 28 June 2016 Received in revised form 28 August 2016 Accepted 4 September 2016 Available online xxxx Background:Glutathione transferases (GSTs) are a family of detoxification enzymes that catalyze the conjugation of glutathione (GSH) to electrophilic compounds.. Methods: A library of alpha class GSTswas constructed by DNA shuffling using the DNA encoding the human glutathione transferase A1-1 (hGSTA1-1) and the rat glutathione transferase A1-1 (rGSTA1-1).. Results: Activity screening of the library allowed the selection of a chimeric enzyme variant (GSTD4) that displayedhigh affinity towards GSHandGSH-Sepharose affinity adsorbent, higher kcat/Km and improved thermal stability, compared to the parent enzymes.

Article Title: Structures of PspAG97A α-glucoside hydrolase reveal a novel mechanism for chloride induced activation.
Article Snippet: Accepted Manuscript Structures of PspAG97A α-Glucoside Hydrolase Reveal a Novel Mechanism for Chloride Induced Activation Chao He, Jing Li, Wei Li, Yi Xue, Zeming Fang, Wei Fang, Xuecheng Zhang, Xiaotang Wang, Yazhong Xiao PII: S1047-8477(16)30198-8 DOI: http://dx.doi.org/10.1016/j.jsb.2016.09.009 Reference: YJSBI 6984 To appear in: Journal of Structural Biology Received Date: 2 June 2016 Revised Date: 4 September 2016 Accepted Date: 15 September 2016 Please cite this article as: He, C., Li, J., Li, W., Xue, Y., Fang, Z., Fang, W., Zhang, X., Wang, X., Xiao, Y., Structures of PspAG97A α-Glucoside Hydrolase Reveal a Novel Mechanism for Chloride Induced Activation, Journal of Structural Biology (2016), doi: http://dx.doi.org/10.1016/j.jsb.2016.09.009 This is a PDF file of an unedited manuscript that has been accepted for publication.. As a service to our customers we are providing this early version of the manuscript.. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final form.

Article Title: Inducer exclusion in Firmicutes: insights into the regulation of a carbohydrate ATP binding cassette transporter from Lactobacillus casei BL23 by the signal transducing protein P-Ser46-HPr.
Article Snippet: To determine the Hill coefficient the computer program GraFit (Erithacus Software Limited, 2012) was used.

Article Title: Purification of β-galactosidase from recombinant Pichia pastoris using aqueous two-phase separation technique
Article Snippet: In the current investigation, aqueous two-phase methodology with polyethylene glycol (PEG) 6000/phosphate salt has been used for single-step purification of β-galactosidase from recombinant Pichia pastoris.. Optimized parameters with 12% (w/w) salt concentration, 25% (w/w) polymer concentration, 0.6 (mg/ml) protein load and 0.1 M ionic concentration resulted in a maximum of 4.7 purification fold with a 97% yield.. The enzyme kinetic study of purified protein revealed Vm and Km of 97.087 (U/mg), 0.027(U/mg), 0.07 (U/mg) and 0.7 (mM), 11.13 (mM), 10.73 (mM) with O-nitrophenyl-β-D-galactopyranoside, lactose and milk substrate, respectively.

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Article Title: Adenosine deaminase - A target for new piperazine derivatives.
Article Snippet: The level of adenosine deaminase (ADA) activity increases in pathological effusions.. Therefore, the concentration of its substrate, anti-inflammatory adenosine, decreases, thereby aggravating inflammation.. Hence, the quest for ADA inhibiting compounds is an actual problem in medicine and pharmacology.



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