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Becton Dickinson computer program cellquest
Effects of 8-Cl-Ado on cell growth and apoptosis. A549 and H1299 cells were exposed to 2 μM 8-Cl-Ado for indicated hours. ( A ) Cell proliferation was evaluated with MTT assay (see materials and methods). Data represent mean ± SD ( n = 3); ( B ) cells exposed to 8-Cl-Ado for 48 h were stained with propidium iodide whose signal was measured by FACScan. Apoptotic cells (subG1/<2N) were assayed by the computer program <t>CELLQuest.</t> Data are representative of three independent experiments; ( C ) a representative Western blotting for Procaspase-3 activation and PARP-1 cleavage in 8-Cl-Ado-exposed cells. β-Actin as a loading control; ( D ) relative levels of Procaspase-3, Procaspase-3-cleaved fragments (p21 and p17), PARP-1 (p115) and its cleaved product (p85) in Western blotting. The blots were screened/quantified with the software Quantity One (Bio Rad) and normalized against β-Actin level, and the ratio of target protein to Actin from control (0 h exposure) cells was designated as “1” (100%). Data represent mean ± SD ( n = 3). * p < 0.05; ** p < 0.01; *** p < 0.001.
Computer Program Cellquest, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/computer+program/cellquest+computer+program/pmc06032248-153-14-17
Average 90 stars, based on 1 article reviews
computer program cellquest - by Bioz Stars, 2026-09
90/100 stars

Images

1) Product Images from "DNA Damage-Response Pathway Heterogeneity of Human Lung Cancer A549 and H1299 Cells Determines Sensitivity to 8-Chloro-Adenosine"

Article Title: DNA Damage-Response Pathway Heterogeneity of Human Lung Cancer A549 and H1299 Cells Determines Sensitivity to 8-Chloro-Adenosine

Journal: International Journal of Molecular Sciences

doi: 10.3390/ijms19061587

Effects of 8-Cl-Ado on cell growth and apoptosis. A549 and H1299 cells were exposed to 2 μM 8-Cl-Ado for indicated hours. ( A ) Cell proliferation was evaluated with MTT assay (see materials and methods). Data represent mean ± SD ( n = 3); ( B ) cells exposed to 8-Cl-Ado for 48 h were stained with propidium iodide whose signal was measured by FACScan. Apoptotic cells (subG1/<2N) were assayed by the computer program CELLQuest. Data are representative of three independent experiments; ( C ) a representative Western blotting for Procaspase-3 activation and PARP-1 cleavage in 8-Cl-Ado-exposed cells. β-Actin as a loading control; ( D ) relative levels of Procaspase-3, Procaspase-3-cleaved fragments (p21 and p17), PARP-1 (p115) and its cleaved product (p85) in Western blotting. The blots were screened/quantified with the software Quantity One (Bio Rad) and normalized against β-Actin level, and the ratio of target protein to Actin from control (0 h exposure) cells was designated as “1” (100%). Data represent mean ± SD ( n = 3). * p < 0.05; ** p < 0.01; *** p < 0.001.
Figure Legend Snippet: Effects of 8-Cl-Ado on cell growth and apoptosis. A549 and H1299 cells were exposed to 2 μM 8-Cl-Ado for indicated hours. ( A ) Cell proliferation was evaluated with MTT assay (see materials and methods). Data represent mean ± SD ( n = 3); ( B ) cells exposed to 8-Cl-Ado for 48 h were stained with propidium iodide whose signal was measured by FACScan. Apoptotic cells (subG1/<2N) were assayed by the computer program CELLQuest. Data are representative of three independent experiments; ( C ) a representative Western blotting for Procaspase-3 activation and PARP-1 cleavage in 8-Cl-Ado-exposed cells. β-Actin as a loading control; ( D ) relative levels of Procaspase-3, Procaspase-3-cleaved fragments (p21 and p17), PARP-1 (p115) and its cleaved product (p85) in Western blotting. The blots were screened/quantified with the software Quantity One (Bio Rad) and normalized against β-Actin level, and the ratio of target protein to Actin from control (0 h exposure) cells was designated as “1” (100%). Data represent mean ± SD ( n = 3). * p < 0.05; ** p < 0.01; *** p < 0.001.

Techniques Used: MTT Assay, Staining, Western Blot, Activation Assay, Control, Software

Related Articles

MTT Assay:

Article Title: A ginseng metabolite, compound K, induces autophagy and apoptosis via generation of reactive oxygen species and activation of JNK in human colon cancer cells
Article Snippet: The sub-G 1 hypodiploid cells were assessed based on histograms generated by the CellQuest and Mod-Fit computer programs (Becton Dickinson).

Article Title: Hwang-Heuk-San induces apoptosis in HCT116 human colorectal cancer cells through the ROS-mediated activation of caspases and the inactivation of the PI3K/Akt signaling pathway.
Article Snippet: The sub-G1 hypodiploid cells were assessed based on histograms generated by the CellQuest and ModFit computer programs (Becton-Dickinson).

Staining:

Article Title: A ginseng metabolite, compound K, induces autophagy and apoptosis via generation of reactive oxygen species and activation of JNK in human colon cancer cells
Article Snippet: The sub-G 1 hypodiploid cells were assessed based on histograms generated by the CellQuest and Mod-Fit computer programs (Becton Dickinson).

Article Title: Hwang-Heuk-San induces apoptosis in HCT116 human colorectal cancer cells through the ROS-mediated activation of caspases and the inactivation of the PI3K/Akt signaling pathway.
Article Snippet: The sub-G1 hypodiploid cells were assessed based on histograms generated by the CellQuest and ModFit computer programs (Becton-Dickinson).

Western Blot:

Article Title: A ginseng metabolite, compound K, induces autophagy and apoptosis via generation of reactive oxygen species and activation of JNK in human colon cancer cells
Article Snippet: The sub-G 1 hypodiploid cells were assessed based on histograms generated by the CellQuest and Mod-Fit computer programs (Becton Dickinson).

Article Title: Hwang-Heuk-San induces apoptosis in HCT116 human colorectal cancer cells through the ROS-mediated activation of caspases and the inactivation of the PI3K/Akt signaling pathway.
Article Snippet: The sub-G1 hypodiploid cells were assessed based on histograms generated by the CellQuest and ModFit computer programs (Becton-Dickinson).

Activation Assay:

Article Title: A ginseng metabolite, compound K, induces autophagy and apoptosis via generation of reactive oxygen species and activation of JNK in human colon cancer cells
Article Snippet: The sub-G 1 hypodiploid cells were assessed based on histograms generated by the CellQuest and Mod-Fit computer programs (Becton Dickinson).

Article Title: Hwang-Heuk-San induces apoptosis in HCT116 human colorectal cancer cells through the ROS-mediated activation of caspases and the inactivation of the PI3K/Akt signaling pathway.
Article Snippet: The sub-G1 hypodiploid cells were assessed based on histograms generated by the CellQuest and ModFit computer programs (Becton-Dickinson).

Control:

Article Title: A ginseng metabolite, compound K, induces autophagy and apoptosis via generation of reactive oxygen species and activation of JNK in human colon cancer cells
Article Snippet: The sub-G 1 hypodiploid cells were assessed based on histograms generated by the CellQuest and Mod-Fit computer programs (Becton Dickinson).

Article Title: Hwang-Heuk-San induces apoptosis in HCT116 human colorectal cancer cells through the ROS-mediated activation of caspases and the inactivation of the PI3K/Akt signaling pathway.
Article Snippet: The sub-G1 hypodiploid cells were assessed based on histograms generated by the CellQuest and ModFit computer programs (Becton-Dickinson).

Software:

Article Title: A ginseng metabolite, compound K, induces autophagy and apoptosis via generation of reactive oxygen species and activation of JNK in human colon cancer cells
Article Snippet: The sub-G 1 hypodiploid cells were assessed based on histograms generated by the CellQuest and Mod-Fit computer programs (Becton Dickinson).

Article Title: Hwang-Heuk-San induces apoptosis in HCT116 human colorectal cancer cells through the ROS-mediated activation of caspases and the inactivation of the PI3K/Akt signaling pathway.
Article Snippet: The sub-G1 hypodiploid cells were assessed based on histograms generated by the CellQuest and ModFit computer programs (Becton-Dickinson).



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