hiscript ⅲrt supermix for qpcr (Vazyme Biotech Co)
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hiscript ⅲrt supermix for qpcr
Hiscript ⅲrt Supermix For Qpcr, supplied by Vazyme Biotech Co, used in various techniques. Bioz Stars score: 99/100, based on 10722 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/computer+code+data+collection+quantitative+pcr+data/HiScript+III+RT+SuperMix+for+qPCR+%2BgDNA+wiper/pmc12031439-112-7-20
Average 99 stars, based on 10722 article reviews
Hiscript ⅲrt Supermix For Qpcr, supplied by Vazyme Biotech Co, used in various techniques. Bioz Stars score: 99/100, based on 10722 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/computer+code+data+collection+quantitative+pcr+data/HiScript+III+RT+SuperMix+for+qPCR+%2BgDNA+wiper/pmc12031439-112-7-20
Average 99 stars, based on 10722 article reviews
hiscript ⅲrt supermix for qpcr - by Bioz Stars,
2026-11
99/100 stars
Images
1) Product Images from "Cinnamaldehyde Inhibits the Replication of Porcine Reproductive and Respiratory Syndrome Virus Type 2 In Vitro"
Article Title: Cinnamaldehyde Inhibits the Replication of Porcine Reproductive and Respiratory Syndrome Virus Type 2 In Vitro
Journal: Viruses
doi: 10.3390/v17040506
Figure Legend Snippet: Cinnamaldehyde suppresses PRRSV infection in Marc-145 cells. ( A , B ) The effect of different concentrations (40, 80, 160 μM) of CA on PRRSV (MOI = 1) replication in Marc-145 cells was investigated by RT-qPCR ( A ) and a viral titer assay ( B ). ( C – F ) Western blotting ( C ) and an immunofluorescence assay ( E ) were used to detect the expression of PRRSV N protein at 24 hpi in the presence of CA (40, 80, 160 μM). Statistical analysis of Western blotting ( D ) and the IFA ( F ). Bar = 100 µm. The data are the results of three independent experiments (means ± SD). Significant differences are denoted by * p < 0.05 and ** p < 0.01.
Techniques Used: Infection, Quantitative RT-PCR, Titer Assay, Western Blot, Immunofluorescence, Expressing
Figure Legend Snippet: Cinnamaldehyde suppresses PRRSV infection in PAM cells. ( A ) The cytotoxicity of CA on PAM cells was evaluated using the CCK-8 assay. The non-toxic concentration of CA was determined to be 80 μM. ( B , C ) The effect of different concentrations (40, 80 μM) of CA on PRRSV (MOI = 1) replication in PAM cells was investigated by RT-qPCR ( B ) and a viral titer assay ( C ). ( D , E ) An immunofluorescence assay ( D ) was used to detect the expression of PRRSV N protein at 24 hpi in the presence of CA (40, 80 μM). Statistical analysis of the IFA ( E ). Bar = 100 µm. The data are the results of three independent experiments (means ± SD). Significant differences are denoted by * p < 0.05 and ** p < 0.01.
Techniques Used: Infection, CCK-8 Assay, Concentration Assay, Quantitative RT-PCR, Titer Assay, Immunofluorescence, Expressing
Figure Legend Snippet: Cinnamaldehyde exerts anti-PRRSV ability in different treatment modes. ( A ) The diagram shows three different administration treatment modes. Pre-treatment: Marc-145 cells were pre-treated with CA at 160 μM at different times (2, 4, 6, 8 h) and then infected with PRRSV for another 2 h. Co-treatment: Marc-145 cells were infected with PRRSV and incubated with CA (160 μM) for 2 h. Post-treatment: Marc-145 cells were infected with PRRSV for 2 h, then treated with CA at 160 μM at different times (2, 4, 6, 8 h). Samples were harvested at 24 hpi. ( B ) RT-qPCR was used to determine viral ORF7 expression. ( C ) The expression of PRRSV N protein was detected by an IFA. ( D ) Statistical analysis of the IFA. ( E ) Cell supernatants were collected for the TCID 50 assay. Bar = 100 µm. The data are the results of three independent experiments (means ± SD). Significant differences are denoted by * p < 0.05 and ** p < 0.01.
Techniques Used: Infection, Incubation, Quantitative RT-PCR, Expressing
Figure Legend Snippet: Cinnamaldehyde blocks the binding, entry, replication, and release of PRRSV. ( A ) A schematic diagram of PRRSV binding, entry, replication, and release. ( B ) A viral binding assay was performed. PRRSV was inoculated in the presence or absence of CA at 4 °C for 2 h. RT-qPCR was used to detect viral ORF7 expression. ( C ) A viral entry assay was performed. After virus binding, cells were transferred to 37 °C for another 2 h. RT-qPCR was used to analyze viral ORF7 expression. ( D ) Viral replication assays were performed. Cells were infected with PRRSV for 4 h (including binding and entry) and then treated with CA for another 6, 12, and 24 h. Cells were collected for RT-qPCR analysis. ( E ) A viral release assay was performed. Marc-145 cells were infected with PRRSV for 24 h. Then, cells were treated with CA for another 8 h. Cell supernatants were collected for the TCID 50 assay. The data are the results of three independent experiments (means ± SD). Significant differences are denoted by * p < 0.05 and ** p < 0.01.
Techniques Used: Binding Assay, Quantitative RT-PCR, Expressing, Virus, Infection, Release Assay
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