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real time pcr system  (Bio-Rad)


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    Structured Review

    Bio-Rad real time pcr system
    Real Time Pcr System, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 99/100, based on 10065 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/computer+code+data+collection+quantitative+pcr+data/CFX384+Touch+Real-Time+PCR+Detection+System+with+Starter+Package/pmc11671672-235-7-15
    Average 99 stars, based on 10065 article reviews
    real time pcr system - by Bioz Stars, 2026-11
    99/100 stars

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    Related Articles

    Real-time Polymerase Chain Reaction:

    Article Title: Decoding the naked mole-rat genome, telomere to telomere
    Article Snippet: Library yield was measured with the Quant-iT dsDNA High Sensitivity assay (Invitrogen Q33120). .. Libraries were validated by qPCR with the KAPA Library Quantification Kit (KK4824) on a Bio-Rad CFX384 Real-Time System. ..

    Article Title: Foundational characterization of tomato fruit RALF peptides reveals structural and functional specialization within the SlRALF family.
    Article Snippet: For cDNA synthesis, 1 μg of total RNA was used for cDNA synthesis using oligo(dT) primers and the iScript cDNA Synthesis Kit (Bio-Rad) following the manufactureŕs instructions. .. RT-qPCR was performed using SsoFast EvaGreen Supermix (Bio-Rad) on a CFX384 Real-Time PCR Detection System (Bio-Rad). ..

    Article Title: Macrophage-Fibroblast Crosstalk Governs Tumor Mechanics, as Revealed by Spatiotemporal Force Mapping in 3D Spheroids.
    Article Snippet: All real-time 4 of 18 PCR primers (Table 2) were purchased from Sigma-Aldrich. .. Quantitative real time PCR was performed with the 2× Sensimix SYBR and Flurescein Kit (Bioline GmBH, Luckenwalde, Germany) using a BioRad CFX384 real-time PCR detection system (BioRad). ..

    Article Title: Yupingfeng alleviates allergic rhinitis by modulating gut microbiota and succinate to inhibit mTOR/S6K signaling.
    Article Snippet: Ethnopharmacological relevance: Yupingfeng granules (YPF), derived from the classic traditional Chinese medicine prescription Yupingfeng powder, have been recommended in clinical practice guidelines for the management of allergic rhinitis (AR), and their clinical efficacy is widely recognized.. However, the underlying pharmacological mechanisms have not been thoroughly investigated.. Aim of the study: This study aimed to investigate whether YPF protects the nasal epithelial barrier and alleviates AR by modulating the gut microbiota and pathways related to its metabolites.

    Article Title: Cold activates a BH2 metabolic network promoting longevity and proteostasis.
    Article Snippet: We then generated cDNA from isolated RNA using the qScript Flex cDNA synthesis kit (Quantabio). .. Quantitative PCR (qPCR) assays were performed with SYBR Green and a 1:20 dilution of cDNA on the CFX384 Real-Time System (Bio-Rad). ..

    Article Title: Neonatal Delivery of Exon Skipping Therapeutics Maximizes Brain Dystrophin Rescue in the Mdx52 Mouse Model of Duchenne Muscular Dystrophy
    Article Snippet: .. Reactions were run under fast cycling conditions on a Bio-Rad CFX384 Touch Real-Time PCR Detection System, and data were analyzed using the absolute copy number method. .. For each sample, copy numbers of the skipped product (exon 50–53) and unskipped product (exon 50–51) were determined using the standards Ex49-54Delta52 and Ex49-54Delta51+52 (gBlocks gene fragments from Integrated DNA Technologies).

    Article Title: Waterpipe Smoke Condensate Impairs p53 Nuclear Localization in Association with HIF-1α Stabilization.
    Article Snippet: .. RT-qPCR was performed using SYBR Green PCR Master Mix (2X; cat. no. 4309155; Applied Biosystems; Thermo Fisher Scientific, Inc.) on a CFX384 Touch Real-Time PCR Detection System (Bio-Rad Laboratories, Inc., Hercules, CA, USA). ..

    Library Quantification:

    Article Title: Decoding the naked mole-rat genome, telomere to telomere
    Article Snippet: Library yield was measured with the Quant-iT dsDNA High Sensitivity assay (Invitrogen Q33120). .. Libraries were validated by qPCR with the KAPA Library Quantification Kit (KK4824) on a Bio-Rad CFX384 Real-Time System. ..

    Quantitative RT-PCR:

    Article Title: Foundational characterization of tomato fruit RALF peptides reveals structural and functional specialization within the SlRALF family.
    Article Snippet: For cDNA synthesis, 1 μg of total RNA was used for cDNA synthesis using oligo(dT) primers and the iScript cDNA Synthesis Kit (Bio-Rad) following the manufactureŕs instructions. .. RT-qPCR was performed using SsoFast EvaGreen Supermix (Bio-Rad) on a CFX384 Real-Time PCR Detection System (Bio-Rad). ..

    Article Title: Diurnal temperature range is a key factor to determine the quality difference between overwinter growing and spring growing tomatoes.
    Article Snippet: Total RNA was extracted from Br+3 samples using a Vazyme RNA extraction kit (Vazyme Biotech Co., Ltd., Nanjing, China). .. One microgram of RNA was reverse-transcribed into cDNA, which was used for qRT-PCR amplification on a Bio-Rad CFX384 system. ..

    Article Title: Waterpipe Smoke Condensate Impairs p53 Nuclear Localization in Association with HIF-1α Stabilization.
    Article Snippet: .. RT-qPCR was performed using SYBR Green PCR Master Mix (2X; cat. no. 4309155; Applied Biosystems; Thermo Fisher Scientific, Inc.) on a CFX384 Touch Real-Time PCR Detection System (Bio-Rad Laboratories, Inc., Hercules, CA, USA). ..

    Reverse Transcription:

    Article Title: Diurnal temperature range is a key factor to determine the quality difference between overwinter growing and spring growing tomatoes.
    Article Snippet: Total RNA was extracted from Br+3 samples using a Vazyme RNA extraction kit (Vazyme Biotech Co., Ltd., Nanjing, China). .. One microgram of RNA was reverse-transcribed into cDNA, which was used for qRT-PCR amplification on a Bio-Rad CFX384 system. ..

    Article Title: Yupingfeng alleviates allergic rhinitis by modulating gut microbiota and succinate to inhibit mTOR/S6K signaling.
    Article Snippet: Ethnopharmacological relevance: Yupingfeng granules (YPF), derived from the classic traditional Chinese medicine prescription Yupingfeng powder, have been recommended in clinical practice guidelines for the management of allergic rhinitis (AR), and their clinical efficacy is widely recognized.. However, the underlying pharmacological mechanisms have not been thoroughly investigated.. Aim of the study: This study aimed to investigate whether YPF protects the nasal epithelial barrier and alleviates AR by modulating the gut microbiota and pathways related to its metabolites.

    Amplification:

    Article Title: Diurnal temperature range is a key factor to determine the quality difference between overwinter growing and spring growing tomatoes.
    Article Snippet: Total RNA was extracted from Br+3 samples using a Vazyme RNA extraction kit (Vazyme Biotech Co., Ltd., Nanjing, China). .. One microgram of RNA was reverse-transcribed into cDNA, which was used for qRT-PCR amplification on a Bio-Rad CFX384 system. ..

    Gene Expression:

    Article Title: Yupingfeng alleviates allergic rhinitis by modulating gut microbiota and succinate to inhibit mTOR/S6K signaling.
    Article Snippet: Ethnopharmacological relevance: Yupingfeng granules (YPF), derived from the classic traditional Chinese medicine prescription Yupingfeng powder, have been recommended in clinical practice guidelines for the management of allergic rhinitis (AR), and their clinical efficacy is widely recognized.. However, the underlying pharmacological mechanisms have not been thoroughly investigated.. Aim of the study: This study aimed to investigate whether YPF protects the nasal epithelial barrier and alleviates AR by modulating the gut microbiota and pathways related to its metabolites.

    SYBR Green Assay:

    Article Title: Cold activates a BH2 metabolic network promoting longevity and proteostasis.
    Article Snippet: We then generated cDNA from isolated RNA using the qScript Flex cDNA synthesis kit (Quantabio). .. Quantitative PCR (qPCR) assays were performed with SYBR Green and a 1:20 dilution of cDNA on the CFX384 Real-Time System (Bio-Rad). ..

    Article Title: Waterpipe Smoke Condensate Impairs p53 Nuclear Localization in Association with HIF-1α Stabilization.
    Article Snippet: .. RT-qPCR was performed using SYBR Green PCR Master Mix (2X; cat. no. 4309155; Applied Biosystems; Thermo Fisher Scientific, Inc.) on a CFX384 Touch Real-Time PCR Detection System (Bio-Rad Laboratories, Inc., Hercules, CA, USA). ..

    Polymerase Chain Reaction:

    Article Title: Waterpipe Smoke Condensate Impairs p53 Nuclear Localization in Association with HIF-1α Stabilization.
    Article Snippet: .. RT-qPCR was performed using SYBR Green PCR Master Mix (2X; cat. no. 4309155; Applied Biosystems; Thermo Fisher Scientific, Inc.) on a CFX384 Touch Real-Time PCR Detection System (Bio-Rad Laboratories, Inc., Hercules, CA, USA). ..



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