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bio rad cfx maestro qpcr software  (Bio-Rad)


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    Structured Review

    Bio-Rad bio rad cfx maestro qpcr software
    Fig. 4. Standard curve as was calculated by the BIO-RAD <t>CFX</t> Maestro <t>qPCR</t> software. (a–k) correspond to standard curve for five DEC virulence genes (aggR; astA; bfpB; escV; invE; lt;pic; sth; stp; stx1; stx2). The x-axis represents the logarithm of varying concentrations of gradient plasmids, while the y-axis denotes the Cq value, also referred to as the threshold cycle number.
    Bio Rad Cfx Maestro Qpcr Software, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 98/100, based on 4019 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/computer+code+data+collection+quantitative+pcr+data/CFX+Maestro+Software/pm40369096-170-7-7
    Average 98 stars, based on 4019 article reviews
    bio rad cfx maestro qpcr software - by Bioz Stars, 2026-11
    98/100 stars

    Images

    1) Product Images from "A TaqMan qPCR for precise detection and quantification of diarrheagenic Escherichia coli."

    Article Title: A TaqMan qPCR for precise detection and quantification of diarrheagenic Escherichia coli.

    Journal: Scientific reports

    doi: 10.1038/s41598-025-96960-1

    Fig. 4. Standard curve as was calculated by the BIO-RAD CFX Maestro qPCR software. (a–k) correspond to standard curve for five DEC virulence genes (aggR; astA; bfpB; escV; invE; lt;pic; sth; stp; stx1; stx2). The x-axis represents the logarithm of varying concentrations of gradient plasmids, while the y-axis denotes the Cq value, also referred to as the threshold cycle number.
    Figure Legend Snippet: Fig. 4. Standard curve as was calculated by the BIO-RAD CFX Maestro qPCR software. (a–k) correspond to standard curve for five DEC virulence genes (aggR; astA; bfpB; escV; invE; lt;pic; sth; stp; stx1; stx2). The x-axis represents the logarithm of varying concentrations of gradient plasmids, while the y-axis denotes the Cq value, also referred to as the threshold cycle number.

    Techniques Used: Software

    Fig. 5. The radar charts presented in (a, b) illustrate the clinical detection results of Taqman qPCR and conventional PCR, respectively. The various colors in the diagram represent different detection results of the virulence genes identified. Based on the graphical representation, it is evident that both detection methods exhibit a high level of consistency in their results.
    Figure Legend Snippet: Fig. 5. The radar charts presented in (a, b) illustrate the clinical detection results of Taqman qPCR and conventional PCR, respectively. The various colors in the diagram represent different detection results of the virulence genes identified. Based on the graphical representation, it is evident that both detection methods exhibit a high level of consistency in their results.

    Techniques Used:

    Related Articles

    Purification:

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    Real-time Polymerase Chain Reaction:

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    Maestro Software:

    Article Title: A fungal clock protein exerts extra-circadian roles in nitrogen metabolism and mitochondrial function.
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    Article Title: Mitochondrial heteroplasmy as a transitional state in the evolution of fungicide resistance.
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    Standard Curve:

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    Generated:

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    Analysis:

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    Software:

    Article Title: The MBW complex regulates floral volatiles in petunia: transcriptional repressor EOBV is a MYB component of the complex.
    Article Snippet: Two-step real-time qPCR was performed on a CFX 1 Opus 384 Real-Time PCR System (Bio-Rad, Hercules, CA, USA) using 2X qPCRBIO SyGreen 2 Blue Mix Hi-ROX (PCR Biosystems, London, UK). .. A standard curve was generated for each gene 3 using dilutions of cDNA samples, and data analysis was performed using Bio-Rad CFX Maestro 4 software. ..

    Article Title: Cellular self-organization orchestrates ferroptosis propagation.
    Article Snippet: 30632780 Experimental models: Cell lines Human: hTERT-RPE1 cells ATCC CRL-4000; RRID: CVCL_4388 (Continued on next page) ll OPEN ACCESS e1 Cell 189, 1–17.e1–e12, December 24, 2026 Article .. REAGENT or RESOURCE SOURCE IDENTIFIER Human: HEK293T cells ATCC CRL-3216; RRID: CVCL_0063 Oligonucleotides Control siRNA Dharmacon Cat# D-001810-01 ON-TARGETplus SMARTpool siRNAs targeting SLC7A11/xCT Dharmacon Cat# L2-007612-01 ON-TARGETplus SMARTpool siRNAs targeting GPX4 Dharmacon Cat# L2-011676-01 Primer: SLC7A11/xCT Forward: 5′-ATGCAGTGGCAGTGACCTTT-3′ Genomics, Taiwan This paper Primer: SLC7A11/xCT Reverse: 5′-GGCAACAAAGATCGGAACTG-3′ Genomics, Taiwan This paper Primer: GPX4 Forward: 5′-GTGAGGCAAGACCGAAGTAAA-3′ Genomics, Taiwan This paper Primer: GPX4 Reverse: 5′-GAACTGGTTACACGGGAAGG-3′ Genomics, Taiwan This paper Primer: β-actin Forward: 5′-CACCATTGGCAATGAGCGGTTC-3′ Genomics, Taiwan This paper Primer: β-actin Reverse: 5′-AGGTCTTTGCGGATGTCCACGT-3′ Genomics, Taiwan This paper Recombinant DNA Plasmid: pCMV-VSV-G Addgene RRID: Addgene_8454 Plasmid: psPAX2 Addgene RRID: Addgene_12260 Plasmid: pLVX-mCherry-C1 Takara Bio Cat# 632561 Plasmid: pRRL-hCMV-SoNar This paper Zhao et al.45 Software and algorithms CFX Maestro software 2.3 v5.3.022.1030 Bio-Rad https://www.bio-rad.com/en-tw/product/cfx- maestro-software-for-cfx-real-time-pcrinstruments MATLAB v2024b MathWorks, Inc. https://www.mathworks.com/ R v4.4.3 R Project https://www.r-project.org/ Python v3.9.12 Python Software Foundation https://www.python.org ImageJ v1.54p Schindelin et al.46 https://imagej.net/software/fiji/ Focus LP v1.0.x Glünder https://www.gluender.de/Miscellanea/MiscTexts/ MiscExcerpts/ReadMe_FL.html Ilastik v.1.4.0 Berg et al.47 https://www.ilastik.org/ Ridge Detection v1.4.1 Wagner et al.48 https://imagej.net/plugins/ridge-detection Image Stitching v3.1.10 Preibisch et al.49 https://imagej.net/plugins/image-stitching BaSIC Peng et al.50 https://sites.imagej.net/BaSiC/ BioVoxxel v.2.6.3 Brocher51 https://imagej.net/plugins/biovoxxel-toolbox Differential Entropy v1.0.2 Ariel and Louzoun52 https://www.mathworks.com/matlabcentral/ fileexchange/105945-differential-continuousentropy-estimator StarDist Schmidt et al.53 https://imagej.net/plugins/stardist Cellpose3 v3 Stringer and Pachitariu54 https://github.com/mouseland/cellpose Cell Ranger v4.0.0 Zheng et al.55 https://www.10xgenomics.com/support/software/ cell-ranger/latest Seurat v4.3.0 Hao et al.56 https://satijalab.org/seurat/ DoubletFinder v2.0 McGinnis et al.57 https://github.com/chris-mcginnis-ucsf/ DoubletFinder SCENIC v1.1.2 Aibar et al.27 and Van de Sande et al.28 https://scenic.aertslab.org/ (Continued on next page) ll OPEN ACCESS Cell 189, 1–17.e1–e12, December 24, 2026 e2 Article .. RPE-1 cells were cultured in RPMI 1640 medium (Gibco, #11875) supplemented with 5% fetal bovine serum (FBS; VWR, VW-97068085).

    Gene Expression:

    Article Title: RNA polymerase I inhibitor BMH-21 promotes mutant p53 reactivation through nuclear zinc mobilization.
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    Article Title: Radiosensitizing Effect of Gold Nanoparticles Stabilized with Sodium Citrate and Thioglycolic Acid on MDA-MB-231 and MCF7 Breast Cancer Cells.
    Article Snippet: .. Data analysis was performed using the Delta Ct Method (2-ddCT) in (Bio-rad CFX Maestro Software v. 3.1), and gene expression levels were calculated relative to the reference gene. ..

    Expressing:

    Article Title: RNA polymerase I inhibitor BMH-21 promotes mutant p53 reactivation through nuclear zinc mobilization.
    Article Snippet: .. Relative gene expression was calculated using the normalized expression (ΔΔCq) analysis in CFX Maestro software (Bio-Rad), with ACTB used as the reference gene and the indicated control condition used as the calibrator. .. Cell viability assays were performed in tissue culture-treated 96-well plates (CellTreat).

    Control:

    Article Title: RNA polymerase I inhibitor BMH-21 promotes mutant p53 reactivation through nuclear zinc mobilization.
    Article Snippet: .. Relative gene expression was calculated using the normalized expression (ΔΔCq) analysis in CFX Maestro software (Bio-Rad), with ACTB used as the reference gene and the indicated control condition used as the calibrator. .. Cell viability assays were performed in tissue culture-treated 96-well plates (CellTreat).

    Article Title: Cellular self-organization orchestrates ferroptosis propagation.
    Article Snippet: 30632780 Experimental models: Cell lines Human: hTERT-RPE1 cells ATCC CRL-4000; RRID: CVCL_4388 (Continued on next page) ll OPEN ACCESS e1 Cell 189, 1–17.e1–e12, December 24, 2026 Article .. REAGENT or RESOURCE SOURCE IDENTIFIER Human: HEK293T cells ATCC CRL-3216; RRID: CVCL_0063 Oligonucleotides Control siRNA Dharmacon Cat# D-001810-01 ON-TARGETplus SMARTpool siRNAs targeting SLC7A11/xCT Dharmacon Cat# L2-007612-01 ON-TARGETplus SMARTpool siRNAs targeting GPX4 Dharmacon Cat# L2-011676-01 Primer: SLC7A11/xCT Forward: 5′-ATGCAGTGGCAGTGACCTTT-3′ Genomics, Taiwan This paper Primer: SLC7A11/xCT Reverse: 5′-GGCAACAAAGATCGGAACTG-3′ Genomics, Taiwan This paper Primer: GPX4 Forward: 5′-GTGAGGCAAGACCGAAGTAAA-3′ Genomics, Taiwan This paper Primer: GPX4 Reverse: 5′-GAACTGGTTACACGGGAAGG-3′ Genomics, Taiwan This paper Primer: β-actin Forward: 5′-CACCATTGGCAATGAGCGGTTC-3′ Genomics, Taiwan This paper Primer: β-actin Reverse: 5′-AGGTCTTTGCGGATGTCCACGT-3′ Genomics, Taiwan This paper Recombinant DNA Plasmid: pCMV-VSV-G Addgene RRID: Addgene_8454 Plasmid: psPAX2 Addgene RRID: Addgene_12260 Plasmid: pLVX-mCherry-C1 Takara Bio Cat# 632561 Plasmid: pRRL-hCMV-SoNar This paper Zhao et al.45 Software and algorithms CFX Maestro software 2.3 v5.3.022.1030 Bio-Rad https://www.bio-rad.com/en-tw/product/cfx- maestro-software-for-cfx-real-time-pcrinstruments MATLAB v2024b MathWorks, Inc. https://www.mathworks.com/ R v4.4.3 R Project https://www.r-project.org/ Python v3.9.12 Python Software Foundation https://www.python.org ImageJ v1.54p Schindelin et al.46 https://imagej.net/software/fiji/ Focus LP v1.0.x Glünder https://www.gluender.de/Miscellanea/MiscTexts/ MiscExcerpts/ReadMe_FL.html Ilastik v.1.4.0 Berg et al.47 https://www.ilastik.org/ Ridge Detection v1.4.1 Wagner et al.48 https://imagej.net/plugins/ridge-detection Image Stitching v3.1.10 Preibisch et al.49 https://imagej.net/plugins/image-stitching BaSIC Peng et al.50 https://sites.imagej.net/BaSiC/ BioVoxxel v.2.6.3 Brocher51 https://imagej.net/plugins/biovoxxel-toolbox Differential Entropy v1.0.2 Ariel and Louzoun52 https://www.mathworks.com/matlabcentral/ fileexchange/105945-differential-continuousentropy-estimator StarDist Schmidt et al.53 https://imagej.net/plugins/stardist Cellpose3 v3 Stringer and Pachitariu54 https://github.com/mouseland/cellpose Cell Ranger v4.0.0 Zheng et al.55 https://www.10xgenomics.com/support/software/ cell-ranger/latest Seurat v4.3.0 Hao et al.56 https://satijalab.org/seurat/ DoubletFinder v2.0 McGinnis et al.57 https://github.com/chris-mcginnis-ucsf/ DoubletFinder SCENIC v1.1.2 Aibar et al.27 and Van de Sande et al.28 https://scenic.aertslab.org/ (Continued on next page) ll OPEN ACCESS Cell 189, 1–17.e1–e12, December 24, 2026 e2 Article .. RPE-1 cells were cultured in RPMI 1640 medium (Gibco, #11875) supplemented with 5% fetal bovine serum (FBS; VWR, VW-97068085).

    Recombinant:

    Article Title: Cellular self-organization orchestrates ferroptosis propagation.
    Article Snippet: 30632780 Experimental models: Cell lines Human: hTERT-RPE1 cells ATCC CRL-4000; RRID: CVCL_4388 (Continued on next page) ll OPEN ACCESS e1 Cell 189, 1–17.e1–e12, December 24, 2026 Article .. REAGENT or RESOURCE SOURCE IDENTIFIER Human: HEK293T cells ATCC CRL-3216; RRID: CVCL_0063 Oligonucleotides Control siRNA Dharmacon Cat# D-001810-01 ON-TARGETplus SMARTpool siRNAs targeting SLC7A11/xCT Dharmacon Cat# L2-007612-01 ON-TARGETplus SMARTpool siRNAs targeting GPX4 Dharmacon Cat# L2-011676-01 Primer: SLC7A11/xCT Forward: 5′-ATGCAGTGGCAGTGACCTTT-3′ Genomics, Taiwan This paper Primer: SLC7A11/xCT Reverse: 5′-GGCAACAAAGATCGGAACTG-3′ Genomics, Taiwan This paper Primer: GPX4 Forward: 5′-GTGAGGCAAGACCGAAGTAAA-3′ Genomics, Taiwan This paper Primer: GPX4 Reverse: 5′-GAACTGGTTACACGGGAAGG-3′ Genomics, Taiwan This paper Primer: β-actin Forward: 5′-CACCATTGGCAATGAGCGGTTC-3′ Genomics, Taiwan This paper Primer: β-actin Reverse: 5′-AGGTCTTTGCGGATGTCCACGT-3′ Genomics, Taiwan This paper Recombinant DNA Plasmid: pCMV-VSV-G Addgene RRID: Addgene_8454 Plasmid: psPAX2 Addgene RRID: Addgene_12260 Plasmid: pLVX-mCherry-C1 Takara Bio Cat# 632561 Plasmid: pRRL-hCMV-SoNar This paper Zhao et al.45 Software and algorithms CFX Maestro software 2.3 v5.3.022.1030 Bio-Rad https://www.bio-rad.com/en-tw/product/cfx- maestro-software-for-cfx-real-time-pcrinstruments MATLAB v2024b MathWorks, Inc. https://www.mathworks.com/ R v4.4.3 R Project https://www.r-project.org/ Python v3.9.12 Python Software Foundation https://www.python.org ImageJ v1.54p Schindelin et al.46 https://imagej.net/software/fiji/ Focus LP v1.0.x Glünder https://www.gluender.de/Miscellanea/MiscTexts/ MiscExcerpts/ReadMe_FL.html Ilastik v.1.4.0 Berg et al.47 https://www.ilastik.org/ Ridge Detection v1.4.1 Wagner et al.48 https://imagej.net/plugins/ridge-detection Image Stitching v3.1.10 Preibisch et al.49 https://imagej.net/plugins/image-stitching BaSIC Peng et al.50 https://sites.imagej.net/BaSiC/ BioVoxxel v.2.6.3 Brocher51 https://imagej.net/plugins/biovoxxel-toolbox Differential Entropy v1.0.2 Ariel and Louzoun52 https://www.mathworks.com/matlabcentral/ fileexchange/105945-differential-continuousentropy-estimator StarDist Schmidt et al.53 https://imagej.net/plugins/stardist Cellpose3 v3 Stringer and Pachitariu54 https://github.com/mouseland/cellpose Cell Ranger v4.0.0 Zheng et al.55 https://www.10xgenomics.com/support/software/ cell-ranger/latest Seurat v4.3.0 Hao et al.56 https://satijalab.org/seurat/ DoubletFinder v2.0 McGinnis et al.57 https://github.com/chris-mcginnis-ucsf/ DoubletFinder SCENIC v1.1.2 Aibar et al.27 and Van de Sande et al.28 https://scenic.aertslab.org/ (Continued on next page) ll OPEN ACCESS Cell 189, 1–17.e1–e12, December 24, 2026 e2 Article .. RPE-1 cells were cultured in RPMI 1640 medium (Gibco, #11875) supplemented with 5% fetal bovine serum (FBS; VWR, VW-97068085).

    Plasmid Preparation:

    Article Title: Cellular self-organization orchestrates ferroptosis propagation.
    Article Snippet: 30632780 Experimental models: Cell lines Human: hTERT-RPE1 cells ATCC CRL-4000; RRID: CVCL_4388 (Continued on next page) ll OPEN ACCESS e1 Cell 189, 1–17.e1–e12, December 24, 2026 Article .. REAGENT or RESOURCE SOURCE IDENTIFIER Human: HEK293T cells ATCC CRL-3216; RRID: CVCL_0063 Oligonucleotides Control siRNA Dharmacon Cat# D-001810-01 ON-TARGETplus SMARTpool siRNAs targeting SLC7A11/xCT Dharmacon Cat# L2-007612-01 ON-TARGETplus SMARTpool siRNAs targeting GPX4 Dharmacon Cat# L2-011676-01 Primer: SLC7A11/xCT Forward: 5′-ATGCAGTGGCAGTGACCTTT-3′ Genomics, Taiwan This paper Primer: SLC7A11/xCT Reverse: 5′-GGCAACAAAGATCGGAACTG-3′ Genomics, Taiwan This paper Primer: GPX4 Forward: 5′-GTGAGGCAAGACCGAAGTAAA-3′ Genomics, Taiwan This paper Primer: GPX4 Reverse: 5′-GAACTGGTTACACGGGAAGG-3′ Genomics, Taiwan This paper Primer: β-actin Forward: 5′-CACCATTGGCAATGAGCGGTTC-3′ Genomics, Taiwan This paper Primer: β-actin Reverse: 5′-AGGTCTTTGCGGATGTCCACGT-3′ Genomics, Taiwan This paper Recombinant DNA Plasmid: pCMV-VSV-G Addgene RRID: Addgene_8454 Plasmid: psPAX2 Addgene RRID: Addgene_12260 Plasmid: pLVX-mCherry-C1 Takara Bio Cat# 632561 Plasmid: pRRL-hCMV-SoNar This paper Zhao et al.45 Software and algorithms CFX Maestro software 2.3 v5.3.022.1030 Bio-Rad https://www.bio-rad.com/en-tw/product/cfx- maestro-software-for-cfx-real-time-pcrinstruments MATLAB v2024b MathWorks, Inc. https://www.mathworks.com/ R v4.4.3 R Project https://www.r-project.org/ Python v3.9.12 Python Software Foundation https://www.python.org ImageJ v1.54p Schindelin et al.46 https://imagej.net/software/fiji/ Focus LP v1.0.x Glünder https://www.gluender.de/Miscellanea/MiscTexts/ MiscExcerpts/ReadMe_FL.html Ilastik v.1.4.0 Berg et al.47 https://www.ilastik.org/ Ridge Detection v1.4.1 Wagner et al.48 https://imagej.net/plugins/ridge-detection Image Stitching v3.1.10 Preibisch et al.49 https://imagej.net/plugins/image-stitching BaSIC Peng et al.50 https://sites.imagej.net/BaSiC/ BioVoxxel v.2.6.3 Brocher51 https://imagej.net/plugins/biovoxxel-toolbox Differential Entropy v1.0.2 Ariel and Louzoun52 https://www.mathworks.com/matlabcentral/ fileexchange/105945-differential-continuousentropy-estimator StarDist Schmidt et al.53 https://imagej.net/plugins/stardist Cellpose3 v3 Stringer and Pachitariu54 https://github.com/mouseland/cellpose Cell Ranger v4.0.0 Zheng et al.55 https://www.10xgenomics.com/support/software/ cell-ranger/latest Seurat v4.3.0 Hao et al.56 https://satijalab.org/seurat/ DoubletFinder v2.0 McGinnis et al.57 https://github.com/chris-mcginnis-ucsf/ DoubletFinder SCENIC v1.1.2 Aibar et al.27 and Van de Sande et al.28 https://scenic.aertslab.org/ (Continued on next page) ll OPEN ACCESS Cell 189, 1–17.e1–e12, December 24, 2026 e2 Article .. RPE-1 cells were cultured in RPMI 1640 medium (Gibco, #11875) supplemented with 5% fetal bovine serum (FBS; VWR, VW-97068085).

    Amplification:

    Article Title: Respiratory Viral Infections in Febrile Children With Lymphoreticular Malignancies: A Single-Centre Descriptive Study.
    Article Snippet: .. Amplification and data analysis were performed on the CFX96 Real‐Time PCR Detection System with CFX Maestro software (Bio‐Rad Laboratories, Inc., Hercules, CA, USA). .. Data were compiled in Microsoft Excel and analyzed using IBM SPSS v20.0.



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