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Shanghai GenePharma lipofectamine complex solution
Lipofectamine Complex Solution, supplied by Shanghai GenePharma, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Article Title: Glutathione Peroxidase 3 induced mitochondria-mediated apoptosis via AMPK /ERK1/2 pathway and resisted autophagy-related ferroptosis via AMPK/mTOR pathway in hyperplastic prostate.
Article Snippet: Following the instructions of Genepharma Ltd. in Suzhou, China, the transfection media were prepared and incubated for 10 min. .. Subsequently, we added 200 μL of lipofectamine (Genepharma Ltd. in Suzhou, China) complex solution to each well and then incubated for 6 h. After that the medium was replaced with fresh medium and incubated for 48–72 h. The sense sequences of si-GPX3 are listed in Additional file 4: Table S4. ..



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UV absorption spectra of PHL solution (A) and zein-PHL mixture solution (B).

Journal: RSC Advances

Article Title: Fabrication of a zein-phloretin complex and nanoparticles: interaction mechanism and characterization

doi: 10.1039/d5ra08898d

Figure Lengend Snippet: UV absorption spectra of PHL solution (A) and zein-PHL mixture solution (B).

Article Snippet: The CD spectra of the zein-PHL complex solutions were measured using a CD spectrometer (J1500, Jasco, Tokyo, Japan), with the zein concentration maintained at 10 μM and the instrument set to scan from 190 to 260 nm.

Techniques:

Effect of PHL on the fluorescence intensity of zein at different temperatures: 298 K (A), 308 K (B), 318 K (C). Stern–Volmer plot of F 0 / F as a function of PHL concentration at different temperatures (D) and plot of log[( F 0 − F )/ F ] as a function of log of PHL concentration (E) in the zein-PHL mixtures at different temperatures. F 0 and F represent the fluorescence intensity in the absence and presence of PHL, respectively.

Journal: RSC Advances

Article Title: Fabrication of a zein-phloretin complex and nanoparticles: interaction mechanism and characterization

doi: 10.1039/d5ra08898d

Figure Lengend Snippet: Effect of PHL on the fluorescence intensity of zein at different temperatures: 298 K (A), 308 K (B), 318 K (C). Stern–Volmer plot of F 0 / F as a function of PHL concentration at different temperatures (D) and plot of log[( F 0 − F )/ F ] as a function of log of PHL concentration (E) in the zein-PHL mixtures at different temperatures. F 0 and F represent the fluorescence intensity in the absence and presence of PHL, respectively.

Article Snippet: The CD spectra of the zein-PHL complex solutions were measured using a CD spectrometer (J1500, Jasco, Tokyo, Japan), with the zein concentration maintained at 10 μM and the instrument set to scan from 190 to 260 nm.

Techniques: Fluorescence, Concentration Assay

CD spectra (A) and secondary structure content (B) of zein-PHL. The PHL concentrations are 0, 10, and 25 µmol L −1 .

Journal: RSC Advances

Article Title: Fabrication of a zein-phloretin complex and nanoparticles: interaction mechanism and characterization

doi: 10.1039/d5ra08898d

Figure Lengend Snippet: CD spectra (A) and secondary structure content (B) of zein-PHL. The PHL concentrations are 0, 10, and 25 µmol L −1 .

Article Snippet: The CD spectra of the zein-PHL complex solutions were measured using a CD spectrometer (J1500, Jasco, Tokyo, Japan), with the zein concentration maintained at 10 μM and the instrument set to scan from 190 to 260 nm.

Techniques: Circular Dichroism

Three-dimensional structure mode of zein-PHL complexes (A); surface structure of PHL in the hydrophobic pocket inside zein (B); binding site of PHL and zein (C).

Journal: RSC Advances

Article Title: Fabrication of a zein-phloretin complex and nanoparticles: interaction mechanism and characterization

doi: 10.1039/d5ra08898d

Figure Lengend Snippet: Three-dimensional structure mode of zein-PHL complexes (A); surface structure of PHL in the hydrophobic pocket inside zein (B); binding site of PHL and zein (C).

Article Snippet: The CD spectra of the zein-PHL complex solutions were measured using a CD spectrometer (J1500, Jasco, Tokyo, Japan), with the zein concentration maintained at 10 μM and the instrument set to scan from 190 to 260 nm.

Techniques: Binding Assay