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complement factor c3  (Cedarlane)


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    Structured Review

    Cedarlane complement factor c3
    Fig. 4. <t>Complement</t> <t>C3</t> deposition in joints. Healthy 5–8-week old female TMwt/wt (A) and TMLeD/LeD (B) mice were killed and knee joints were processed for immunohistochemical staining to detect complement factor C3 deposition. These sections are representative of three mice. No C3 was detectable in the joints of TMwt/wt mice (A). C3 was readily observed on the articular surface (small arrows) and in the bone marrow space of TMLeD/LeD mice (large arrows) (B). Higher power views of the articular surface are shown in the right upper corner of each panel.
    Complement Factor C3, supplied by Cedarlane, used in various techniques. Bioz Stars score: 85/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/complement+c3/Anti-Mouse+Complement+Component+C3%2C+Purified+(Clone+RmC11H9)+(rat+IgG2a)/pm16879225-65-48-53
    Average 85 stars, based on 6 article reviews
    complement factor c3 - by Bioz Stars, 2026-11
    85/100 stars

    Images

    1) Product Images from "The lectin-like domain of thrombomodulin interferes with complement activation and protects against arthritis."

    Article Title: The lectin-like domain of thrombomodulin interferes with complement activation and protects against arthritis.

    Journal: Journal of thrombosis and haemostasis : JTH

    doi: 10.1111/j.1538-7836.2006.02033.x

    Fig. 4. Complement C3 deposition in joints. Healthy 5–8-week old female TMwt/wt (A) and TMLeD/LeD (B) mice were killed and knee joints were processed for immunohistochemical staining to detect complement factor C3 deposition. These sections are representative of three mice. No C3 was detectable in the joints of TMwt/wt mice (A). C3 was readily observed on the articular surface (small arrows) and in the bone marrow space of TMLeD/LeD mice (large arrows) (B). Higher power views of the articular surface are shown in the right upper corner of each panel.
    Figure Legend Snippet: Fig. 4. Complement C3 deposition in joints. Healthy 5–8-week old female TMwt/wt (A) and TMLeD/LeD (B) mice were killed and knee joints were processed for immunohistochemical staining to detect complement factor C3 deposition. These sections are representative of three mice. No C3 was detectable in the joints of TMwt/wt mice (A). C3 was readily observed on the articular surface (small arrows) and in the bone marrow space of TMLeD/LeD mice (large arrows) (B). Higher power views of the articular surface are shown in the right upper corner of each panel.

    Techniques Used: Immunohistochemical staining, Staining

    Related Articles

    Staining:

    Article Title: The receptor BLT1 is essential on neutrophils in a mouse model of mucous membrane pemphigoid
    Article Snippet: .. Briefly, 6 μm cryosections of perilesional skin were washed with 0.01 M PBS (pH 7.2), fixed with acetone, and stained with Alexa Fluor 594 AffiniPure Goat Anti-Rabbit IgG (Jackson ImmunoResearch; 1:500; 112-585-167) or purified rat anti-mouse complement C3 (Cedarlane Labs; 1:200; CL7503AP) for 1 hour at RT. .. Sections to be stained for C3 were washed and incubated with Alexa Fluor 488 AffiniPure Goat anti-rabbit IgG for 1 hour at RT.

    Article Title: The receptor BLT1 is essential on neutrophils in a mouse model of mucous membrane pemphigoid.
    Article Snippet: .. Briefly, 6 μm cryosections of perilesional skin were washed with 0.01 M PBS (pH 7.2), fixed with acetone, and stained with Alexa Fluor 594 AffiniPure Goat Anti-Rabbit IgG (Jackson ImmunoResearch; 1:500; 112-585-167) or purified rat antimouse complement C3 (Cedarlane Labs; 1:200; CL7503AP) for 1 hour at RT. .. Sections to be stained for C3 were washed and incubated with Alexa Fluor 488 AffiniPure Goat anti-rabbit IgG for 1 hour at RT.

    Article Title: Subcapsular encounter and complement-dependent transport of immune complexes by lymph node B cells.
    Article Snippet: .. Tissue sections 10–30 mm in thickness were stained with rat monoclonal anti–mouse C3 (CL7503AP; Cedarlane), anti-CR1 (8C12) or MOMA-1 hybridoma supernatant and were detected with fluorescein isothiocyanate–conjugated anti–rat IgG (heavy plus light) F(ab¢)2 (Jackson Immunosearch). ..

    Purification:

    Article Title: The receptor BLT1 is essential on neutrophils in a mouse model of mucous membrane pemphigoid
    Article Snippet: .. Briefly, 6 μm cryosections of perilesional skin were washed with 0.01 M PBS (pH 7.2), fixed with acetone, and stained with Alexa Fluor 594 AffiniPure Goat Anti-Rabbit IgG (Jackson ImmunoResearch; 1:500; 112-585-167) or purified rat anti-mouse complement C3 (Cedarlane Labs; 1:200; CL7503AP) for 1 hour at RT. .. Sections to be stained for C3 were washed and incubated with Alexa Fluor 488 AffiniPure Goat anti-rabbit IgG for 1 hour at RT.

    Article Title: The receptor BLT1 is essential on neutrophils in a mouse model of mucous membrane pemphigoid.
    Article Snippet: .. Briefly, 6 μm cryosections of perilesional skin were washed with 0.01 M PBS (pH 7.2), fixed with acetone, and stained with Alexa Fluor 594 AffiniPure Goat Anti-Rabbit IgG (Jackson ImmunoResearch; 1:500; 112-585-167) or purified rat antimouse complement C3 (Cedarlane Labs; 1:200; CL7503AP) for 1 hour at RT. .. Sections to be stained for C3 were washed and incubated with Alexa Fluor 488 AffiniPure Goat anti-rabbit IgG for 1 hour at RT.

    Western Blot:

    Article Title: Immune evasion of Enterococcus faecalis by an extracellular gelatinase that cleaves C3 and iC3b.
    Article Snippet: .. Furthermore, the proteolytic effect of GelE on C3 in mouse serum was assessed via an immunoblotting assay conducted with an anti-mouse C3 mAb (CL7503AP: Cedarlane Laboratories) (Fig. 6B). ..

    Immunohistochemistry:

    Article Title: The Neuroprotective Effect of Genetic Mannose-binding Lectin Deficiency is not Sustained in the Sub-acute Phase of Stroke
    Article Snippet: Antibodies and Reagents Primary antibodies used for Western blot include rat anti-mouse monoclonal C3a antibody(BD-Pharmingen, San Diego, CA)(1:500 dilution); rabbit polyclonal anti-mouse IgM antibody(Novus Biologicals, Littleton, CO)(1:1000); goat anti-mouse MBL-1 antibody(R&D Systems, Minneapolis, MN)(1:1000); goat anti-mouse MBL-2 antibody(R&D Systems, Minneapolis, MN)(1:1000); rat anti-mouse monoclonal CD68(AbD Serotec, Raleigh, NC)(1:2000); rabbit polyclonal anti-mouse Factor-B antibody(Santa Cruz Biotechnology, Santa Cruz, CA)(1:1000); and goat polyclonal anti-mouse C1q-A antibody(Santa Cruz Biotechnology, Santa Cruz, CA)(1:1000). .. Primary antibodies used for immunohistochemistry include monoclonal anti-MAP-2(Sigma-Aldrich, St. Louis, MO)(1:600); rat anti-mouse monoclonal complement component C3 antibody(Cedarlane, Burlington, NC)(1:200); goat anti-mouse polyclonal IgM(AbD Serotec, Raleigh, NC)(1:200); rat anti-mouse monoclonal CD68(AbD Serotec, Raleigh, NC)(1:200); goat anti-mouse MBL-1 antibody(R&D Systems, Minneapolis, MN)(1:200); goat anti-mouse MBL-2 antibody(R&D Systems, Minneapolis, MN)(1:200); rabbit polyclonal anti-human von Willebrand Factor(DakoCytomation, Ely, Cambridgeshire, UK)(1:100). ..



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    Image Search Results


    Schematic illustration of in vivo tumor immunotherapy enhanced by mRNA/HNPs through intravenous injection. H18 lipid, DOPE, cholesterol, DMG-PEG 2000 and mRNA were mixed to form mRNA/H 18 NPs with the special multilamellar concentric nanostructures. Following intravenous administration, mRNA/H 18 NPs demonstrated preferential adsorption of complement C3 proteins to form a characteristic protein corona, resulting in specific mRNA transfection in the spleen, especially in splenic dendritic cells. When encapsulating tumor antigen-encoding mRNA, the mRNA/H 18 NPs achieved precise transfection of the antigen mRNA in splenic dendritic cells. This targeted delivery stimulated dendritic cell maturation and subsequent antigen presentation, initiating robust T cell priming. The activated antigen-specific cytotoxic T lymphocytes then infiltrated into tumor tissues, ultimately inducing tumor cell elimination.

    Journal: Bioactive Materials

    Article Title: Splenic dendritic cell-targeting mRNA transfection of H-type ionizable lipid-based LNPs for enhancing tumor immunotherapy

    doi: 10.1016/j.bioactmat.2026.02.018

    Figure Lengend Snippet: Schematic illustration of in vivo tumor immunotherapy enhanced by mRNA/HNPs through intravenous injection. H18 lipid, DOPE, cholesterol, DMG-PEG 2000 and mRNA were mixed to form mRNA/H 18 NPs with the special multilamellar concentric nanostructures. Following intravenous administration, mRNA/H 18 NPs demonstrated preferential adsorption of complement C3 proteins to form a characteristic protein corona, resulting in specific mRNA transfection in the spleen, especially in splenic dendritic cells. When encapsulating tumor antigen-encoding mRNA, the mRNA/H 18 NPs achieved precise transfection of the antigen mRNA in splenic dendritic cells. This targeted delivery stimulated dendritic cell maturation and subsequent antigen presentation, initiating robust T cell priming. The activated antigen-specific cytotoxic T lymphocytes then infiltrated into tumor tissues, ultimately inducing tumor cell elimination.

    Article Snippet: Mouse Complement C3 was purchased from MedChemExpress.

    Techniques: In Vivo, Injection, Adsorption, Transfection, Immunopeptidomics

    In vivo splenic DC-specific transfection of mRNA/H 18 NPs and in vitro protein corona analysis of mRNA/H 18 NPs. (A) EGFP protein expression in splenic cell subsets of C57BL/6J mice 24 h post intravenous injection of different formulations. (B) The top 5 most abundant plasma proteins adsorbed on mRNA/H 18 NPs (C3: Complement C3; Ighm: Immunoglobulin heavy constant mu; Hbat1: Alpha-globin; Itih4: Inter alpha-trypsin inhibitor, heavy chain 4; Cnn2: Calponin). (C) Heatmap plot of major proteins in the protein corona adsorbed on mRNA/MC3-LNPs and mRNA/H 18 NPs. PBS group was used as a negative control. (D) Quantification of major adsorbed protein categories of different formulations. (E) Complement C3 abundance in protein corona adsorbed on mRNA/MC3-LNPs and mRNA/H 18 NPs. (F) Bioluminescence images of major organs and (G) Quantification of total bioluminescence flux in the spleen from C57BL/6J mice 6 h after intravenous injection of mLuc/H 18 NPs (mLuc dose of 0.25 mg kg −1 ). Mice were pre-treated with cobra venom factor (CVF) or PBS. (H) Fluorescence quantification of Cy5 mRNA delivered by uncoated or complement C3-coated Cy5-mRNA/H 18 NPs in BMDCs. BMDCs were pre-incubated with anti-CD11b (CR3) or anti-IgG blocking antibody. (I) Bioluminescence intensity of luciferase protein translated from mRNA delivered by uncoated or complement C3-coated mLuc/H 18 NPs in BMDCs. BMDCs were pre-incubated with anti-CD11b (CR3) or anti-IgG blocking antibody. Data were shown as mean ± SD (n = 3).

    Journal: Bioactive Materials

    Article Title: Splenic dendritic cell-targeting mRNA transfection of H-type ionizable lipid-based LNPs for enhancing tumor immunotherapy

    doi: 10.1016/j.bioactmat.2026.02.018

    Figure Lengend Snippet: In vivo splenic DC-specific transfection of mRNA/H 18 NPs and in vitro protein corona analysis of mRNA/H 18 NPs. (A) EGFP protein expression in splenic cell subsets of C57BL/6J mice 24 h post intravenous injection of different formulations. (B) The top 5 most abundant plasma proteins adsorbed on mRNA/H 18 NPs (C3: Complement C3; Ighm: Immunoglobulin heavy constant mu; Hbat1: Alpha-globin; Itih4: Inter alpha-trypsin inhibitor, heavy chain 4; Cnn2: Calponin). (C) Heatmap plot of major proteins in the protein corona adsorbed on mRNA/MC3-LNPs and mRNA/H 18 NPs. PBS group was used as a negative control. (D) Quantification of major adsorbed protein categories of different formulations. (E) Complement C3 abundance in protein corona adsorbed on mRNA/MC3-LNPs and mRNA/H 18 NPs. (F) Bioluminescence images of major organs and (G) Quantification of total bioluminescence flux in the spleen from C57BL/6J mice 6 h after intravenous injection of mLuc/H 18 NPs (mLuc dose of 0.25 mg kg −1 ). Mice were pre-treated with cobra venom factor (CVF) or PBS. (H) Fluorescence quantification of Cy5 mRNA delivered by uncoated or complement C3-coated Cy5-mRNA/H 18 NPs in BMDCs. BMDCs were pre-incubated with anti-CD11b (CR3) or anti-IgG blocking antibody. (I) Bioluminescence intensity of luciferase protein translated from mRNA delivered by uncoated or complement C3-coated mLuc/H 18 NPs in BMDCs. BMDCs were pre-incubated with anti-CD11b (CR3) or anti-IgG blocking antibody. Data were shown as mean ± SD (n = 3).

    Article Snippet: Mouse Complement C3 was purchased from MedChemExpress.

    Techniques: In Vivo, Transfection, In Vitro, Expressing, Injection, Clinical Proteomics, Negative Control, Combined Bisulfite Restriction Analysis Assay, Fluorescence, Incubation, Blocking Assay, Luciferase