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Trevigen cometchip protocol
Cometchip Protocol, supplied by Trevigen, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cometchip+protocol/cometchip/pmc10030420-110-1-9
Average 90 stars, based on 1 article reviews
cometchip protocol - by Bioz Stars, 2026-10
90/100 stars

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Related Articles

Alkaline Single Cell Gel Electrophoresis:

Article Title: Substituting meat for mycoprotein reduces genotoxicity and increases the abundance of beneficial microbes in the gut: Mycomeat, a randomised crossover control trial
Article Snippet: The supernatants, representing the faecal water fraction, were aliquoted into sterile 1.5 ml Eppendorf tubes and stored at − 80 °C for analysis of faecal water genotoxicity and metabolites. .. The human colon adenocarcinoma cell line Caco-2 was used to test faecal water genotoxicity using a CometChip assay (Trevigen), a high-throughput version of the standard alkaline comet assay [ ]. ..

Lysis:

Article Title: Trabectedin derails transcription-coupled nucleotide excision repair to induce DNA breaks in highly transcribed genes
Article Snippet: .. 30 μm CometChip ® (catalog no. 4250-096-01), low melting agarose (catalog no. 4250-500-02), and lysis solution (catalog no. 4250-500-01) were from Trevigen. .. 1X SYBR Gold (Invitrogen) was used for staining and comets were imaged with 4X magnification on a fluorescence microscope (BX53, Olympus).

Suspension:

Article Title: Comparative genotoxicity assessment of ortho-phthalaldehyde using human in vitro organotypic airway epithelial cultures and standard in vitro genotoxicity assays.
Article Snippet: ortho-Phthalaldehyde (OPA) is a high-level chemical disinfectant used for sterilizing heat-sensitive medical equipment.. OPA has been reported to be an airway irritant in animal studies, in human case reports, and in a human in vitro organotypic air–liquid-interface (ALI) airway culture model; there are also data indicating that it may be a direct-acting bacterial mutagen.. In this study, we investigated the genotoxic potential of OPA using human ALI airway cultures, human lymphoblastoid TK6 cells, and the Ames test.

other:

Article Title: In vitro Safety Assessment of Extracts and Compounds From Plants as Sunscreen Ingredients.
Article Snippet: This work investigated the safety of extracts obtained from plants growing in Colombia, which have previously shown UV-filter/ antigenotoxic properties.. The compounds in plant extracts obtained by the supercritical fluid (CO2) extraction method were identified using gas chromatography coupled to mass spectrometry (GC/MS) analysis.. Cytotoxicity measured as cytotoxic concentration 50% (CC50) and genotoxicity of the plant extracts and some compounds were studied in human fibroblasts using the trypan blue exclusion assay and the Comet assay, respectively.

Article Title: Targeting the COP9 signalosome overcomes platinum resistance in ovarian cancer through two distinct genome stability mechanisms
Article Snippet: Fluorescent images were captured on a Leica DMi8 Thunder imager, and ImageJ was used for analysis.

Article Title: DNA Repair Capacity and Clinicopathological Characteristics in Puerto Rican Hispanic/Latino Patients with Metastatic Castration-Resistant Prostate Cancer
Article Snippet: These were thawed in batches of five samples to perform DRC measurements using CometChip (R&D Systems, Minneapolis, MN, USA).


Single Cell Gel Electrophoresis:

Article Title: Alkylation of nucleobases by 2-chloro- N,N -diethylethanamine hydrochloride (CDEAH) sensitizes PARP1 -deficient tumors
Article Snippet: Apoptotic cell death was quantified using an Annexin V Alexa FluorTM 488 conjugate (A13201, Thermo Fisher Scientific) and a BD FACSVerse instrument with FlowJo software (version 10) according to the manufacturer’s instructions. .. The comet assay was performed using a CometChip ® (Trevigen) according to the manufacturer’s instructions. ..



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Trevigen cometchip protocol
Cometchip Protocol, supplied by Trevigen, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cometchip+protocol/cometchip/pmc10030420-110-1-9
Average 90 stars, based on 1 article reviews
cometchip protocol - by Bioz Stars, 2026-10
90/100 stars
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