tango pcdna3 1 library (Addgene inc)

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Addgene inc tango pcdna3 1 library
Tango Pcdna3 1 Library, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 18 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/codon+optimized+dor+tango+construct/pCDNA3%2E1+(-)+%2B+codon+optimized+Brn3a+(Plasmid+%2386829)/pm38898705-91-10-17
Average 93 stars, based on 18 article reviews

tango pcdna3 1 library - by Bioz Stars, 2026-11

93/100 stars

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Plasmid Preparation:

Article Title: Innovative design and comprehensive characterization of a valine-enhanced lipid nanoparticle-coated mRNA vaccine targeting the SARS-CoV-2 S-RBD antigen.
Article Snippet: .. Firstly, codon optimized plasmid (1–10 ng/μl, Addgene) cloned in DH5α bacteria and plasmid DNA isolation was performed using plasmid DNA isolation kit (NucleoSpin® Plasmid Mini Kit, Macherey–Nagel). .. The designed forward primers (Primer 1 and Primer 3) and 3′UTR sequence was gently mixed with T7 ATP (1 μM, Thermo Fisher Scientific), T4 polynucleotide kinase buffer (1X, NEB), T4 polynucleotide kinase (0.2 U/ μl, NEB) to be phosphorylated.

Article Title: Innovative design and comprehensive characterization of a valine-enhanced lipid nanoparticle-coated mRNA vaccine targeting the SARS-CoV-2 S-RBD antigen
Article Snippet: .. Firstly, codon optimized plasmid (1–10 ng/μl, Addgene) cloned in DH5α bacteria and plasmid DNA isolation was performed using plasmid DNA isolation kit (NucleoSpin® Plasmid Mini Kit, Macherey–Nagel). .. The designed forward primers (Primer 1 and Primer 3) and 3′UTR sequence was gently mixed with T7 ATP (1 μM, Thermo Fisher Scientific), T4 polynucleotide kinase buffer (1X, NEB), T4 polynucleotide kinase (0.2 U/μl, NEB) to be phosphorylated.

Clone Assay:

Article Title: Innovative design and comprehensive characterization of a valine-enhanced lipid nanoparticle-coated mRNA vaccine targeting the SARS-CoV-2 S-RBD antigen
Article Snippet: .. Firstly, codon optimized plasmid (1–10 ng/μl, Addgene) cloned in DH5α bacteria and plasmid DNA isolation was performed using plasmid DNA isolation kit (NucleoSpin® Plasmid Mini Kit, Macherey–Nagel). .. The designed forward primers (Primer 1 and Primer 3) and 3′UTR sequence was gently mixed with T7 ATP (1 μM, Thermo Fisher Scientific), T4 polynucleotide kinase buffer (1X, NEB), T4 polynucleotide kinase (0.2 U/μl, NEB) to be phosphorylated.

Bacteria:

Article Title: Innovative design and comprehensive characterization of a valine-enhanced lipid nanoparticle-coated mRNA vaccine targeting the SARS-CoV-2 S-RBD antigen
Article Snippet: .. Firstly, codon optimized plasmid (1–10 ng/μl, Addgene) cloned in DH5α bacteria and plasmid DNA isolation was performed using plasmid DNA isolation kit (NucleoSpin® Plasmid Mini Kit, Macherey–Nagel). .. The designed forward primers (Primer 1 and Primer 3) and 3′UTR sequence was gently mixed with T7 ATP (1 μM, Thermo Fisher Scientific), T4 polynucleotide kinase buffer (1X, NEB), T4 polynucleotide kinase (0.2 U/μl, NEB) to be phosphorylated.

DNA Extraction:

Article Title: Innovative design and comprehensive characterization of a valine-enhanced lipid nanoparticle-coated mRNA vaccine targeting the SARS-CoV-2 S-RBD antigen
Article Snippet: .. Firstly, codon optimized plasmid (1–10 ng/μl, Addgene) cloned in DH5α bacteria and plasmid DNA isolation was performed using plasmid DNA isolation kit (NucleoSpin® Plasmid Mini Kit, Macherey–Nagel). .. The designed forward primers (Primer 1 and Primer 3) and 3′UTR sequence was gently mixed with T7 ATP (1 μM, Thermo Fisher Scientific), T4 polynucleotide kinase buffer (1X, NEB), T4 polynucleotide kinase (0.2 U/μl, NEB) to be phosphorylated.

Construct:

Article Title: Pharmacological Mechanism of the Non-Hallucinogenic 5-HT2A agonist Ariadne and Analogs
Article Snippet: Stably-expressing 5-HT 2A/2B/2C receptor Flp-In 293 T-Rex Tetracycline inducible system (Invitrogen, mycoplasma-free) were used for calcium flux assays. .. Constructs used for these assays were derived from the codon-optimized Tango pcDNA3.1 library (Addgene) with V2tail/TEV/tTA encoding regions deleted to yield “de-Tango” constructs, and then shuttled into pcDNA5/FRT/TO using Gibson Assembly. .. Cell lines were maintained in high-glucose DMEM (VWR) containing 10% FBS (Life Technologies), 10 μg/mL Blasticidin (GoldBio), and 100 μg/mL Hygromycin B (GoldBio).

Article Title: Pharmacological Mechanism of the Non-Hallucinogenic 5-HT2A agonist Ariadne and Analogs
Article Snippet: All BRET assays were conducted using BRET2 in HEK293T cells (ATCC CRL-11268; mycoplasma-free), which were subcultured in high-glucose DMEM (VWR) supplemented with 10% FBS (Life Technologies). .. Constructs in G protein-dissociation BRET assays were derived from the codon-optimized Tango pcDNA3.1 library (Addgene) with V2tail/TEV/tTA encoding regions deleted to yield “de-Tango” constructs. ..

Derivative Assay:

Bioluminescence Resonance Energy Transfer:

Expressing:

Article Title: A single amino acid in the Salmonella effector SarA/SteE triggers supraphysiological activation of STAT3 for anti-inflammatory target gene expression
Article Snippet: .. The pcDNA3.1-FLAG -sarA , pcDNA3.1-FLAG -sarA:gp130 chimera, pcDNA3.1-FLAG- gp130dimer , pcDNA3.1-FLAG -gp130:sarA chimera codon-optimized for mammalian expression were previously described in and available from Addgene. .. Site-directed mutagenesis was carried out using Quikchange Lightning Site-Directed Mutagenesis Kit (Agilent, # 210518) (primers in Table S2).

other:

Article Title: Bioisosteric analogs of MDMA: Improving the pharmacological profile?
Article Snippet: Stably- expressing 5- HT2A/2B/2C receptor Flp- In 293 T- Rex Tetracycline inducible system (Invitrogen, mycoplasma- free) were used for calcium flux assays (Lewis et al., 2023).

Review